BACKGROUND Bacillus Calmette-Guérin (BCG) is taken into account a promising dwell bacterial supply system. Nonetheless, a number of proposals for rBCG vaccines haven’t progressed, primarily because of the limitations of the accessible expression techniques. OBJECTIVES To acquire a set of mycobacterial vectors utilizing a spread of promoters with completely different strengths based mostly on a typical spine, beforehand proven to be steady. METHODS Mycobacterial expression vectors based mostly on the pLA71 vector as spine, have been obtained inserting completely different promoters (PAN, PαAg, PHsp60, PBlaF* and PL5) and the inexperienced fluorescence protein (GFP) as reporter gene, to judge options equivalent to their relative strengths, and the in vitro (inside macrophages) and in vivo stability.
FINDINGS The relative fluorescence noticed with the completely different vectors confirmed growing energy of the promoters: PAN was the weakest in each Mycobacterium smegmatis and BCG and PBlaF* was larger than PHsp60 in BCG. The relative fluorescence noticed in a macrophage cell line confirmed that PBlaF* and PHsp60 have been comparable. It was not doable to acquire strains reworked with the extrachromosomal expression vector containing the PL5 in both species. MAIN CONCLUSION We have now obtained a set of doubtless steady mycobacterial vectors with a prepare of expression ranges, for use within the growth of rBCG vaccines.
Building of E. coli-Mycobacterium shuttle vectors with a wide range of expression techniques and polypeptide tags for gene expression in mycobacteria.
PDGFB-expressing mesenchymal stem cells enhance human hematopoietic stem cell engraftment in immunodeficient mice.
Filter Paper Simple 110mm - PK100 |
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FIL0122 | Scientific Laboratory Supplies | PK100 | EUR 8.1 |
Filter Paper Simple 125mm - PK100 |
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FIL0124 | Scientific Laboratory Supplies | PK100 | EUR 9.45 |
Filter Paper Simple 150mm - PK100 |
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FIL0126 | Scientific Laboratory Supplies | PK100 | EUR 12.15 |
Blunt Simple Cloning Kit |
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20-abx098060 | Abbexa |
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Blunt Simple Cloning Kit |
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abx098060-100l | Abbexa | 100 µl | EUR 737.5 |
Blunt Simple Cloning Kit |
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abx098060-1ml | Abbexa | 1 ml | Ask for price |
Blunt Simple Cloning Kit |
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abx098060-200l | Abbexa | 200 µl | EUR 950 |
pUC57- Simple- gRNA backbone |
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PVT11371 | Lifescience Market | 2 ug | EUR 361.2 |
pUC57 Simple-PVT1 Plasmid |
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PVTB00511 | Lifescience Market | 2 ug | EUR 427.2 |
pUC57 Simple-Pdcd4 Plasmid |
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PVTB70003 | Lifescience Market | 2 ug | EUR 427.2 |
Simple add on polarizer - EACH |
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MIC5830 | Scientific Laboratory Supplies | EACH | EUR 62.28 |
Filter Paper Simple 90mm - PK100 |
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FIL0120 | Scientific Laboratory Supplies | PK100 | EUR 6.75 |
Fast, Simple & Efficient Cloning Kit without competent cell |
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GWB-PS10F8 | GenWay Biotech | 20reactions | Ask for price |
Fast, Simple & Efficient Cloning Kit without competent cell |
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GWB-PSEBB3 | GenWay Biotech | 40reactions | Ask for price |
Fast, Simple & Efficient Cloning Kit without competent cell |
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GWB-6F5A1D | GenWay Biotech | 100reactions | Ask for price |
Fast, Simple & Efficient Cloning Kit without competent cell |
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GWB-768936 | GenWay Biotech | 10reactions | Ask for price |
SiMPLe Protein Labeling Kit (Sortase Mediated Protein Ligation) |
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79392 | BPS Bioscience | 3 units of Sortase | EUR 560 |
Description: Sortases catalize a transpeptidation reaction, where the enzyme cleaves the amide bond between the threonine and glycine of the sortase recognition sequence (LPXTG for S. aureus Sortase A), generating a thioacyl intermediate. Subsequently, this intermediate is resolved by the N-terminus of an oligoglycine nucleophile, creating a new peptide bond that links the substrate to the incoming nucleophile. The Sortagging Kit takes advantage of this reaction to label virtually any recombinant antibody or protein containing the sortase recognition sequence. Labeling efficiency of greater than 90% is achieved by taking advantage of the highly active Sortase 5Δ Pentamutant. Simply combine your protein of interest with Sortase 5Δ and a polyglycine label, and incubate for 1 hour at 37°C. Purification columns are included to remove Sortase 5Δ and excess label. The kit also includes Ubiquitin-LPETGH6 and GGG-Clover as positive controls._x000D_ |
OASA05978-1ML - Herpes Simple Virus 1/2 Antibody - HRP Conjugated |
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OASA05978-1ML | Aviva Systems Biology | 1ml | EUR 499 |
Simple Cyanide Standard for Ion Chromatography and Calibration of Ion Selective Electrodes - 500ML |
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RSCN9-2X | Scientific Laboratory Supplies | 500ML | EUR 168.75 |
Simple Cyanide Standard for Ion Chromatography and Calibration of Ion Selective Electrodes - 125ML |
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RSCN9-2Y | Scientific Laboratory Supplies | 125ML | EUR 108 |
Blood Genomic DNA Extraction Mini Kit (50prep), (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK-001 | Favorgen | 50 preps | EUR 180 |
Blood Genomic DNA Extraction Mini Kit (100prep), (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK-001-1 | Favorgen | 100 preps | EUR 232.8 |
Blood Genomic DNA Extraction Mini Kit (300prep), (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK-001-2 | Favorgen | 300 preps | EUR 406.8 |
Blood Genomic DNA Extraction MAXI Kit (10prep) (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK-003 | Favorgen | 10 preps | EUR 242.4 |
Blood Genomic DNA Extraction MAXI Kit (24prep) (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK-003-1 | Favorgen | 24 preps | EUR 379.2 |
Blood Genomic DNA Extraction Mini Kit (50prep), (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK_001 | Favorgen | 1 kit | EUR 39.5 |
Blood Genomic DNA Extraction Mini Kit (100prep), (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK_001-1 | Favorgen | 1 kit | EUR 73 |
Blood Genomic DNA Extraction Mini Kit (300prep), (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK_001-2 | Favorgen | 1 kit | EUR 269 |
Blood Genomic DNA Extraction MAXI Kit (10prep) (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK_003 | Favorgen | 1 kit | EUR 80 |
Blood Genomic DNA Extraction MAXI Kit (24prep) (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK_003-1 | Favorgen | 1 kit | EUR 170 |
Blood / Cultured Cells Genomic DNA Extraction Midi Kit (25prep) (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK-002 | Favorgen | 25 preps | EUR 241.2 |
Blood / Cultured Cells Genomic DNA Extraction Midi Kit (25prep) (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK_002 | Favorgen | 1 kit | EUR 78.5 |
Herpes simplex type 1 |
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PCR-H426-PCRH42648D | Bioingentech | PCR-H426-48D | EUR 230 |
Herpes simplex type 1 |
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PCR-H426-PCRH42696D | Bioingentech | PCR-H426-96D | EUR 312 |
Herpes simplex type 2 |
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PCR-H427-PCRH42748D | Bioingentech | PCR-H427-48D | EUR 230 |
Herpes simplex type 2 |
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PCR-H427-PCRH42796D | Bioingentech | PCR-H427-96D | EUR 312 |
Herpes simplex type 1 |
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Oneq-H426-OneqH426100D | Bioingentech | Oneq-H426-100D | EUR 515 |
Herpes simplex type 1 |
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Oneq-H426-OneqH426150D | Bioingentech | Oneq-H426-150D | EUR 594 |
Herpes simplex type 1 |
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Oneq-H426-OneqH42650D | Bioingentech | Oneq-H426-50D | EUR 413 |
Herpes simplex type 2 |
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Oneq-H427-OneqH427100D | Bioingentech | Oneq-H427-100D | EUR 515 |
Herpes simplex type 2 |
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Oneq-H427-OneqH427150D | Bioingentech | Oneq-H427-150D | EUR 594 |
Herpes simplex type 2 |
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Oneq-H427-OneqH42750D | Bioingentech | Oneq-H427-50D | EUR 413 |
Herpes Simplex Virus 2 (HSV 2) |
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GWB-814A13 | GenWay Biotech | 1 ml | Ask for price |
Herpes Simplex Virus 2 (HSV 2) gG 95% |
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GWB-99F0D2 | GenWay Biotech | 0.1 mg | Ask for price |
Herpes Simplex Virus 1 (HSV 1) |
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GWB-7E1F5B | GenWay Biotech | 1 ml | Ask for price |
Herpes Simplex Virus 1 gD (HSV 1 gD) |
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GWB-F99012 | GenWay Biotech | 1 mg | Ask for price |
Herpes Simplex Virus 2 (HSV 2) |
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GWB-FA122B | GenWay Biotech | 1 ml | Ask for price |
Herpes Simplex Virus 1 (HSV 1) |
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GWB-D60086 | GenWay Biotech | 1 ml | Ask for price |
Herpes simplex type 1 PCR kit |
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PCR-H426-48D | Bioingentech | 50T | EUR 543.6 |
Herpes simplex type 1 PCR kit |
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PCR-H426-96D | Bioingentech | 100T | EUR 686.4 |
Herpes simplex type 2 PCR kit |
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PCR-H427-48D | Bioingentech | 50T | EUR 543.6 |
Herpes simplex type 2 PCR kit |
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PCR-H427-96D | Bioingentech | 100T | EUR 686.4 |
Herpes Simplex Virus-1 gD |
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PR27127 | Neuromics | 500 ug | EUR 999.6 |
Herpes Simplex Virus-1 gG |
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PR27128 | Neuromics | 500 ug | EUR 999.6 |
Herpes Simplex Virus-2 gD |
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PR27129 | Neuromics | 500 ug | EUR 999.6 |
Herpes simplex type 1 RT PCR kit |
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RTq-H426-100D | Bioingentech | 100T | EUR 860.4 |
Herpes simplex type 1 RT PCR kit |
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RTq-H426-150D | Bioingentech | 150T | EUR 969.6 |
Herpes simplex type 1 RT PCR kit |
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RTq-H426-50D | Bioingentech | 50T | EUR 717.6 |
Herpes simplex type 2 RT PCR kit |
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RTq-H427-100D | Bioingentech | 100T | EUR 860.4 |
Herpes simplex type 2 RT PCR kit |
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RTq-H427-150D | Bioingentech | 150T | EUR 969.6 |
Herpes simplex type 2 RT PCR kit |
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RTq-H427-50D | Bioingentech | 50T | EUR 717.6 |
Herpes Simplex Virus-1 gD |
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PR27127-B | Neuromics | each | EUR 540 |
Herpes Simplex Virus-1 gG |
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PR27128-B | Neuromics | each | EUR 540 |
Herpes Simplex Virus-2 gD |
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PR27129-B | Neuromics | each | EUR 540 |
Herpes Simplex Virus-2 gG |
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rAP-5322 | Angio Proteomie | Inquiry | Ask for price |
Herpes simplex Virus 1 (HSV 1) IgG |
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DEHSV1G0500 | Demeditec Diagnostics | 96 | EUR 92 |
Herpes simplex Virus 1 (HSV 1) IgM |
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DEHSV1M0500 | Demeditec Diagnostics | 96 | EUR 92 |
Herpes simplex Virus 2 (HSV 2) IgG |
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DEHSV2G0540 | Demeditec Diagnostics | 96 | EUR 92 |
Herpes simplex Virus 2 (HSV 2) IgM |
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DEHSV2M0540 | Demeditec Diagnostics | 96 | EUR 92 |
Herpes simplex Virus 1+2 (HSV) IgG |
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DEHSVG0250 | Demeditec Diagnostics | 96 | EUR 92 |
Herpes simplex Virus 1+2 (HSV) IgM |
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DEHSVM0250 | Demeditec Diagnostics | 96 | EUR 92 |
Herpes Simplex 1 IgA ELISA kit |
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55R-IB79241 | Fitzgerald | 96 wells | EUR 382.8 |
Description: ELISA kit for the detection of Herpes Simplex 1 IgA in the research laboratory |
Herpes Simplex 1 IgG ELISA kit |
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55R-IB79242 | Fitzgerald | 96 wells | EUR 379.2 |
Description: ELISA kit for the detection of Herpes Simplex 1 IgG in the research laboratory |
Herpes Simplex 1 IgM ELISA kit |
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55R-IB79243 | Fitzgerald | 96 wells | EUR 379.2 |
Description: ELISA kit for the detection of Herpes Simplex 1 IgM in the research laboratory |
Herpes Simplex 1/2 IgA ELISA kit |
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55R-IB79244 | Fitzgerald | 96 wells | EUR 379.2 |
Description: ELISA kit for the detection of Herpes Simplex 1/2 IgA in the research laboratory |
Herpes Simplex 1/2 IgG ELISA kit |
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55R-IB79245 | Fitzgerald | 96 wells | EUR 379.2 |
Description: ELISA kit for the detection of Herpes Simplex 1/2 IgG in the research laboratory |
Herpes Simplex 1/2 IgM ELISA kit |
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55R-IB79246 | Fitzgerald | 96 wells | EUR 379.2 |
Description: ELISA kit for the detection of Herpes Simplex 1/2 IgM in the research laboratory |
Herpes Simplex 2 IgA ELISA kit |
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55R-IB79247 | Fitzgerald | 96 wells | EUR 379.2 |
Description: ELISA kit for the detection of Herpes Simplex 2 IgA in the research laboratory |
Herpes Simplex 2 IgG ELISA kit |
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55R-IB79248 | Fitzgerald | 96 wells | EUR 379.2 |
Description: ELISA kit for the detection of Herpes Simplex 2 IgG in the research laboratory |
Herpes Simplex 2 IgM ELISA kit |
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55R-IB79249 | Fitzgerald | 96 wells | EUR 379.2 |
Description: ELISA kit for the detection of Herpes Simplex 2 IgM in the research laboratory |
Herpes Simplex Virus 2 (HSV 2) gG2 |
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GWB-A5E360 | GenWay Biotech | 1 mg | Ask for price |
Simplettes - 250mm - PK1000 |
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SEW7114 | Scientific Laboratory Supplies | PK1000 | EUR 152.55 |
Herpes simplex virus, type 1 |
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9010 | Virostat | each | EUR 440 |
Description: glycoprotein gD |
Herpes simplex virus, type 2 |
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9011 | Virostat | each | EUR 440 |
Description: gycoprotein gD |
Herpes simplex virus, type 1 |
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9014 | Virostat | each | EUR 370 |
Description: glycoprotein G |
Herpes Simplex Virus 1 (HSV 1) ecto gD 95% |
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GWB-DCF544 | GenWay Biotech | 0.1 mg | Ask for price |
NATIVE HERPES SIMPLEX VIRUS 2 |
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GWB-614440 | GenWay Biotech | 1 ml | Ask for price |
NATIVE HERPES SIMPLEX VIRUS 1 |
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GWB-8E3AF9 | GenWay Biotech | 1 ml | Ask for price |
HSV1 (Herpes Simplex Virus Type I)(N/A), 0.2mg/mL |
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BNUB1800-100 | Biotium | 1uL | EUR 225 |
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application |
HSV1 (Herpes Simplex Virus Type I)(N/A), 0.2mg/mL |
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BNUB1800-500 | Biotium | 1uL | EUR 485 |
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application |
HSV1 (Herpes Simplex Virus Type I)(N/A), 1mg/mL |
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BNUM1800-50 | Biotium | 1uL | EUR 396 |
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application |
Herpes Simplex Virus 1 Antigen |
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HS105-1000 | The Native Antigen Company | 1.0 | EUR 1178.79 |
Description: HSV-1 particles from a cell lysate are purified by sucrose gradient ultracentrifugation. |
Herpes Simplex Virus 1 Antigen |
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HS105-200 | The Native Antigen Company | 0.2 | EUR 294.7 |
Description: HSV-1 particles from a cell lysate are purified by sucrose gradient ultracentrifugation. |
Herpes Simplex Virus 2 Antigen |
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HS205-1000 | The Native Antigen Company | 1.0 | EUR 1178.79 |
Description: HSV-2 particles from a cell lysate are purified by sucrose gradient ultracentrifugation. |
Herpes Simplex Virus 2 Antigen |
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HS205-200 | The Native Antigen Company | 0.2 | EUR 294.7 |
Description: HSV-2 particles from a cell lysate are purified by sucrose gradient ultracentrifugation. |
HERPES SIMPLEX VIRUS 1 Protein |
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GWB-Q00415 | GenWay Biotech | 1 ml | Ask for price |
HERPES SIMPLEX VIRUS 2 Protein |
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GWB-Q00417 | GenWay Biotech | 1 ml | Ask for price |
HERPES SIMPLEX VIRUS 2 Protein |
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GWB-Q00418 | GenWay Biotech | 0.2 ml | Ask for price |
Mouse Anti Herpes Simplex Virus gD (0195) |
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MAB12274-100 | The Native Antigen Company | 0.1 | EUR 275.56 |
Description: Mouse monoclonal antibody specific for Herpes Simplex virus gD (0192) |
Mouse Anti Herpes Simplex Virus gD (0197) |
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MAB12275-100 | The Native Antigen Company | 0.1 | EUR 275.56 |
Description: Mouse monoclonal antibody specific for Herpes Simplex virus gD (0197) |
Mouse Anti Herpes Simplex Virus 2 gD (0192) |
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MAB12276-100 | The Native Antigen Company | 0.1 | EUR 275.56 |
Description: Mouse monoclonal antibody specific for Herpes Simplex virus gD (0192) |
Herpes simplex Virus 1 Antigen |
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BA1051VS | Genovis AB | 1mg | EUR 877 |
Herpes simplex Virus 2 Antigen |
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BA1052VS | Genovis AB | 1mg | EUR 877 |
Herpes simplex Virus 1 IgG ELISA Kit |
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ESR1051G | Genovis AB | 96 Tests | EUR 362 |
Herpes simplex Virus 1 IgM ELISA Kit |
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ESR1051M | Genovis AB | 96 Tests | EUR 362 |
Herpes simplex Virus 2 IgG ELISA Kit |
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ESR1052G | Genovis AB | 96 Tests | EUR 362 |
Herpes simplex Virus 2 IgM ELISA Kit |
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ESR1052M | Genovis AB | 96 Tests | EUR 362 |
Herpes Simplex Virus-2 gG Protein |
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20-abx260324 | Abbexa |
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Herpes Simplex Virus-1 gD Protein |
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20-abx260325 | Abbexa |
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Herpes Simplex Virus-2 gD Protein |
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20-abx260326 | Abbexa |
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Herpes Simplex Virus-2 gG Protein |
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20-abx260327 | Abbexa |
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Herpes Simplex Virus-1 gG Protein |
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20-abx260328 | Abbexa |
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Herpes Simplex Virus-2 gB Protein |
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20-abx260330 | Abbexa |
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Herpes Simplex Virus 2 Antibody (gG) |
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GWB-374257 | GenWay Biotech | 1 mg | Ask for price |
Herpex Simplex Virus 1/2 Antibody |
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GWB-3E9759 | GenWay Biotech | 1 ml | Ask for price |
Herpes simplex Type-2 Antibody |
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GWB-43A7C8 | GenWay Biotech | 1 ml | Ask for price |
Herpes simplex Type-2 Antibody |
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GWB-5A8586 | GenWay Biotech | 1 ml | Ask for price |
Herpes simplex Type-1 Antibody |
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GWB-5EB3C0 | GenWay Biotech | 1 ml | Ask for price |
Herpes Simplex Virus gG2 Protein |
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HSV-gG2-100 | The Native Antigen Company | 0.1 | EUR 473.31 |
Description: Native HSV2 gG2 protein purified from HSV2 infected Baby Hamster Kidney (BHK) cells. |
Herpes Simplex Virus gG2 Protein |
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HSV-gG2-1000 | The Native Antigen Company | 1.0 | EUR 1422.47 |
Description: Native HSV2 gG2 protein purified from HSV2 infected Baby Hamster Kidney (BHK) cells. |
Herpes Simplex Virus Antibody |
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GWB-244DEA | GenWay Biotech | 1 ml | Ask for price |
Herpes simplex Type-2 Antibody |
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GWB-194C60 | GenWay Biotech | 1 ml | Ask for price |
Human Simplex Virus 1 (HSV-1) IgM ELISA Kit |
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IHUHSV1IGMKT | Innovative research | each | EUR 528 |
Description: Human Simplex Virus 1 (HSV-1) IgM ELISA Kit |
Herpes simplex Type-1 Antibody |
|||
GWB-8EEBAC | GenWay Biotech | 1 ml | Ask for price |