
BACKGROUND Bacillus Calmette-Guérin (BCG) is taken into account a promising dwell bacterial supply system. Nonetheless, a number of proposals for rBCG vaccines haven’t progressed, primarily because of the limitations of the accessible expression techniques. OBJECTIVES To acquire a set of mycobacterial vectors utilizing a spread of promoters with completely different strengths based mostly on a typical spine, beforehand proven to be steady. METHODS Mycobacterial expression vectors based mostly on the pLA71 vector as spine, have been obtained inserting completely different promoters (PAN, PαAg, PHsp60, PBlaF* and PL5) and the inexperienced fluorescence protein (GFP) as reporter gene, to judge options equivalent to their relative strengths, and the in vitro (inside macrophages) and in vivo stability.
FINDINGS The relative fluorescence noticed with the completely different vectors confirmed growing energy of the promoters: PAN was the weakest in each Mycobacterium smegmatis and BCG and PBlaF* was larger than PHsp60 in BCG. The relative fluorescence noticed in a macrophage cell line confirmed that PBlaF* and PHsp60 have been comparable. It was not doable to acquire strains reworked with the extrachromosomal expression vector containing the PL5 in both species. MAIN CONCLUSION We have now obtained a set of doubtless steady mycobacterial vectors with a prepare of expression ranges, for use within the growth of rBCG vaccines.
Building of E. coli-Mycobacterium shuttle vectors with a wide range of expression techniques and polypeptide tags for gene expression in mycobacteria.

Characterisation of Alternative Expression Vectors for Recombinant Bacillus Calmette-Guérin as Live Bacterial Delivery Systems
PDGFB-expressing mesenchymal stem cells enhance human hematopoietic stem cell engraftment in immunodeficient mice.
Simple add on polarizer |
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MIC5830 | Scientific Laboratory Supplies | EACH | EUR 55.36 |
Blunt Simple Cloning Kit |
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20-abx098060 | Abbexa |
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Filter Paper Simple 90mm |
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FIL0120 | Scientific Laboratory Supplies | PK100 | EUR 6 |
pUC57- Simple- gRNA backbone |
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PVT11371 | Lifescience Market | 2 ug | EUR 361.2 |
pUC57 Simple-PVT1 Plasmid |
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PVTB00511 | Lifescience Market | 2 ug | EUR 427.2 |
pUC57 Simple-Pdcd4 Plasmid |
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PVTB70003 | Lifescience Market | 2 ug | EUR 427.2 |
SiMPLe Protein Labeling Kit (Sortase Mediated Protein Ligation) |
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79392 | BPS Bioscience | 3 units of Sortase | EUR 560 |
Description: Sortases catalize a transpeptidation reaction, where the enzyme cleaves the amide bond between the threonine and glycine of the sortase recognition sequence (LPXTG for S. aureus Sortase A), generating a thioacyl intermediate. Subsequently, this intermediate is resolved by the N-terminus of an oligoglycine nucleophile, creating a new peptide bond that links the substrate to the incoming nucleophile. The Sortagging Kit takes advantage of this reaction to label virtually any recombinant antibody or protein containing the sortase recognition sequence. Labeling efficiency of greater than 90% is achieved by taking advantage of the highly active Sortase 5Δ Pentamutant. Simply combine your protein of interest with Sortase 5Δ and a polyglycine label, and incubate for 1 hour at 37°C. Purification columns are included to remove Sortase 5Δ and excess label. The kit also includes Ubiquitin-LPETGH6 and GGG-Clover as positive controls._x000D_ |
Simple Cyanide Standard for Ion Chromatography and Calibration of Ion Selective Electrodes |
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RSCN9-2X | Scientific Laboratory Supplies | 500ML | EUR 150 |
Simple Cyanide Standard for Ion Chromatography and Calibration of Ion Selective Electrodes |
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RSCN9-2Y | Scientific Laboratory Supplies | 125ML | EUR 96 |
Blood Genomic DNA Extraction Mini Kit (50prep), (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK-001 | Favorgen | 50 preps | EUR 180 |
Blood Genomic DNA Extraction Mini Kit (100prep), (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK-001-1 | Favorgen | 100 preps | EUR 232.8 |
Blood Genomic DNA Extraction Mini Kit (300prep), (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK-001-2 | Favorgen | 300 preps | EUR 406.8 |
Blood Genomic DNA Extraction MAXI Kit (10prep) (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK-003 | Favorgen | 10 preps | EUR 242.4 |
Blood Genomic DNA Extraction MAXI Kit (24prep) (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK-003-1 | Favorgen | 24 preps | EUR 379.2 |
Blood Genomic DNA Extraction Mini Kit (50prep), (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK_001 | Favorgen | 1 kit | EUR 39.5 |
Blood Genomic DNA Extraction Mini Kit (100prep), (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK_001-1 | Favorgen | 1 kit | EUR 73 |
Blood Genomic DNA Extraction Mini Kit (300prep), (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK_001-2 | Favorgen | 1 kit | EUR 269 |
Blood Genomic DNA Extraction MAXI Kit (10prep) (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK_003 | Favorgen | 1 kit | EUR 80 |
Blood Genomic DNA Extraction MAXI Kit (24prep) (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK_003-1 | Favorgen | 1 kit | EUR 170 |
Blood / Cultured Cells Genomic DNA Extraction Midi Kit (25prep) (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK-002 | Favorgen | 25 preps | EUR 241.2 |
Blood / Cultured Cells Genomic DNA Extraction Midi Kit (25prep) (With Proteinase K Powder, for wide applications and simple procedure) |
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FABGK_002 | Favorgen | 1 kit | EUR 78.5 |
Simplettes - 250mm |
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SEW7114 | Scientific Laboratory Supplies | PK1000 | EUR 135.6 |
Herpes simplex type 1 |
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PCR-H426-PCRH42648D | Bioingentech | PCR-H426-48D | EUR 230 |
Herpes simplex type 1 |
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PCR-H426-PCRH42696D | Bioingentech | PCR-H426-96D | EUR 312 |
Herpes simplex type 2 |
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PCR-H427-PCRH42748D | Bioingentech | PCR-H427-48D | EUR 230 |
Herpes simplex type 2 |
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PCR-H427-PCRH42796D | Bioingentech | PCR-H427-96D | EUR 312 |
Herpes simplex type 1 |
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Oneq-H426-OneqH426100D | Bioingentech | Oneq-H426-100D | EUR 515 |
Herpes simplex type 1 |
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Oneq-H426-OneqH426150D | Bioingentech | Oneq-H426-150D | EUR 594 |
Herpes simplex type 1 |
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Oneq-H426-OneqH42650D | Bioingentech | Oneq-H426-50D | EUR 413 |
Herpes simplex type 2 |
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Oneq-H427-OneqH427100D | Bioingentech | Oneq-H427-100D | EUR 515 |
Herpes simplex type 2 |
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Oneq-H427-OneqH427150D | Bioingentech | Oneq-H427-150D | EUR 594 |
Herpes simplex type 2 |
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Oneq-H427-OneqH42750D | Bioingentech | Oneq-H427-50D | EUR 413 |
Herpes Simplex Virus-1 gD |
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PR27127 | Neuromics | 500 ug | EUR 999.6 |
Herpes Simplex Virus-1 gG |
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PR27128 | Neuromics | 500 ug | EUR 999.6 |
Herpes Simplex Virus-2 gD |
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PR27129 | Neuromics | 500 ug | EUR 999.6 |
Herpes simplex type 1 PCR kit |
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PCR-H426-48D | Bioingentech | 50T | EUR 543.6 |
Herpes simplex type 1 PCR kit |
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PCR-H426-96D | Bioingentech | 100T | EUR 686.4 |
Herpes simplex type 2 PCR kit |
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PCR-H427-48D | Bioingentech | 50T | EUR 543.6 |
Herpes simplex type 2 PCR kit |
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PCR-H427-96D | Bioingentech | 100T | EUR 686.4 |
Herpes simplex type 1 RT PCR kit |
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RTq-H426-100D | Bioingentech | 100T | EUR 860.4 |
Herpes simplex type 1 RT PCR kit |
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RTq-H426-150D | Bioingentech | 150T | EUR 969.6 |
Herpes simplex type 1 RT PCR kit |
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RTq-H426-50D | Bioingentech | 50T | EUR 717.6 |
Herpes simplex type 2 RT PCR kit |
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RTq-H427-100D | Bioingentech | 100T | EUR 860.4 |
Herpes simplex type 2 RT PCR kit |
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RTq-H427-150D | Bioingentech | 150T | EUR 969.6 |
Herpes simplex type 2 RT PCR kit |
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RTq-H427-50D | Bioingentech | 50T | EUR 717.6 |
Herpes Simplex 1 IgA ELISA kit |
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55R-IB79241 | Fitzgerald | 96 wells | EUR 382.8 |
Description: ELISA kit for the detection of Herpes Simplex 1 IgA in the research laboratory |
Herpes Simplex 1 IgG ELISA kit |
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55R-IB79242 | Fitzgerald | 96 wells | EUR 379.2 |
Description: ELISA kit for the detection of Herpes Simplex 1 IgG in the research laboratory |
Herpes Simplex 1 IgM ELISA kit |
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55R-IB79243 | Fitzgerald | 96 wells | EUR 379.2 |
Description: ELISA kit for the detection of Herpes Simplex 1 IgM in the research laboratory |
Herpes Simplex 1/2 IgA ELISA kit |
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55R-IB79244 | Fitzgerald | 96 wells | EUR 379.2 |
Description: ELISA kit for the detection of Herpes Simplex 1/2 IgA in the research laboratory |
Herpes Simplex 1/2 IgG ELISA kit |
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55R-IB79245 | Fitzgerald | 96 wells | EUR 379.2 |
Description: ELISA kit for the detection of Herpes Simplex 1/2 IgG in the research laboratory |
Herpes Simplex 1/2 IgM ELISA kit |
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55R-IB79246 | Fitzgerald | 96 wells | EUR 379.2 |
Description: ELISA kit for the detection of Herpes Simplex 1/2 IgM in the research laboratory |
Herpes Simplex 2 IgA ELISA kit |
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55R-IB79247 | Fitzgerald | 96 wells | EUR 379.2 |
Description: ELISA kit for the detection of Herpes Simplex 2 IgA in the research laboratory |
Herpes Simplex 2 IgG ELISA kit |
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55R-IB79248 | Fitzgerald | 96 wells | EUR 379.2 |
Description: ELISA kit for the detection of Herpes Simplex 2 IgG in the research laboratory |
Herpes Simplex 2 IgM ELISA kit |
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55R-IB79249 | Fitzgerald | 96 wells | EUR 379.2 |
Description: ELISA kit for the detection of Herpes Simplex 2 IgM in the research laboratory |
Simplette Straws 177mm 3.6-3.7mm |
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SOC3112 | Scientific Laboratory Supplies | PK2000 | EUR 100.8 |
HSV1 (Herpes Simplex Virus Type I)(N/A), 0.2mg/mL |
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BNUB1800-100 | Biotium | 1uL | EUR 225 |
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application |
HSV1 (Herpes Simplex Virus Type I)(N/A), 0.2mg/mL |
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BNUB1800-500 | Biotium | 1uL | EUR 485 |
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application |
HSV1 (Herpes Simplex Virus Type I)(N/A), 1mg/mL |
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BNUM1800-50 | Biotium | 1uL | EUR 396 |
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application |
Herpes Simplex Virus-2 gG Protein |
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20-abx260324 | Abbexa |
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Herpes Simplex Virus-1 gD Protein |
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20-abx260325 | Abbexa |
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Herpes Simplex Virus-2 gD Protein |
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20-abx260326 | Abbexa |
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Herpes Simplex Virus-2 gG Protein |
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20-abx260327 | Abbexa |
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Herpes Simplex Virus-1 gG Protein |
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20-abx260328 | Abbexa |
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Herpes Simplex Virus-2 gB Protein |
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20-abx260330 | Abbexa |
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Herpes simplex type 1 One-Step PCR kit |
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Oneq-H426-100D | Bioingentech | 100T | EUR 1039.2 |
Herpes simplex type 1 One-Step PCR kit |
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Oneq-H426-150D | Bioingentech | 150T | EUR 1177.2 |
Herpes simplex type 1 One-Step PCR kit |
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Oneq-H426-50D | Bioingentech | 50T | EUR 861.6 |
Herpes simplex type 2 One-Step PCR kit |
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Oneq-H427-100D | Bioingentech | 100T | EUR 1039.2 |
Herpes simplex type 2 One-Step PCR kit |
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Oneq-H427-150D | Bioingentech | 150T | EUR 1177.2 |
Herpes simplex type 2 One-Step PCR kit |
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Oneq-H427-50D | Bioingentech | 50T | EUR 861.6 |
Human Simplex Virus 1 (HSV-1) IgM ELISA Kit |
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IHUHSV1IGMKT | Innovative research | each | EUR 528 |
Description: Human Simplex Virus 1 (HSV-1) IgM ELISA Kit |
Human Herpes simplex virus 2 ELISA Kit |
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ELA-E0792h | Lifescience Market | 96 Tests | EUR 988.8 |
HSV1 (Herpes Simplex Virus Type I)(10A3), 0.2mg/mL |
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BNUB1793-100 | Biotium | 1uL | EUR 225 |
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application |
HSV1 (Herpes Simplex Virus Type I)(10A3), 0.2mg/mL |
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BNUB1793-500 | Biotium | 1uL | EUR 485 |
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application |
HSV1 (Herpes Simplex Virus Type I)(HSVI/2095), 0.2mg/mL |
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BNUB2095-100 | Biotium | 1uL | EUR 225 |
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application |
HSV1 (Herpes Simplex Virus Type I)(HSVI/2095), 0.2mg/mL |
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BNUB2095-500 | Biotium | 1uL | EUR 485 |
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application |
HSV1 (Herpes Simplex Virus Type I)(10A3), 1mg/mL |
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BNUM1793-50 | Biotium | 1uL | EUR 396 |
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application |
HSV1 (Herpes Simplex Virus Type I)(HSV1/1934), 1mg/mL |
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BNUM1934-50 | Biotium | 1uL | EUR 396 |
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application |
HSV1 (Herpes Simplex Virus Type I)(HSVI/2095), 1mg/mL |
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BNUM2095-50 | Biotium | 1uL | EUR 396 |
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application |
Herpes Simplex Virus-2 VP13 / 14 Protein |
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20-abx260331 | Abbexa |
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Herpes Simplex Virus-2 VP22 Protein |
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20-abx260332 | Abbexa |
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Herpes Simplex Virus 2 (HSV-2) Antibody |
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abx021858-1mg | Abbexa | 1 mg | EUR 718.8 |
Herpes Simplex Virus 2 (HSV-2) Antibody |
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abx023038-1ml | Abbexa | 1 ml | EUR 644.4 |
Pipettor 500ul for Simplette Straws |
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PIP1520 | Scientific Laboratory Supplies | EACH | EUR 249.37 |
Pipettor 1000ul for Simplette Straws |
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PIP1530 | Scientific Laboratory Supplies | EACH | EUR 242.4 |
Herpes Simplex Virus 2 (HSV-2) Antibody (HRP) |
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abx023040-1ml | Abbexa | 1 ml | EUR 811.2 |
Human Herpes Simplex Virus 2 (HSV-2) ELISA Kit |
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abx053156-96tests | Abbexa | 96 tests | EUR 801.6 |
HSV1 (Herpes Simplex Virus Type I) ICP8(HSVI/2045), 0.2mg/mL |
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BNUB2045-100 | Biotium | 1uL | EUR 225 |
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application |
HSV1 (Herpes Simplex Virus Type I) ICP8(HSVI/2045), 0.2mg/mL |
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BNUB2045-500 | Biotium | 1uL | EUR 485 |
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application |
HSV1 (Herpes Simplex Virus Type I) ICP8(HSVI/2045), 1mg/mL |
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BNUM2045-50 | Biotium | 1uL | EUR 396 |
Description: Primary and secondary antibodies for multiple methodologyimmunostaining detection application |
Human Herpes simplex virus 1 (HSV1) ELISA Kit |
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SL0871Hu_1 | Sunlong | 96 Tests | Ask for price |
Herpes Simplex Virus-8 Mosaic Protein |
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20-abx260329 | Abbexa |
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Mouse antibody for Herpes simplex virus |
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129 | Virostat | 100 ug | EUR 386.26 |
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, type 2 gG for WB, ELISA. |
Mouse antibody for Herpes simplex virus |
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125 | Virostat | 100 ug | EUR 386.26 |
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, type 2 gG for WB, ELISA. |
Mouse antibody for Herpes simplex virus |
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126 | Virostat | 100 ug | EUR 386.26 |
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, type 2 gG for WB, ELISA. |
Mouse antibody for Herpes simplex virus |
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128 | Virostat | 100 ug | EUR 386.26 |
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, type 2 gG for WB, ELISA. |
Mouse antibody for Herpes simplex virus |
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112 | Virostat | 100 ug | EUR 386.26 |
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, type 1 gE for WB, ELISA. |
Mouse antibody for Herpes simplex virus |
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116 | Virostat | 100 ug | EUR 386.26 |
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, type 2 gG for WB, ELISA. |
Mouse antibody for Herpes simplex virus |
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117 | Virostat | 100 ug | EUR 425.4 |
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, 1&2 Thymidine kinase for WB, ELISA. |
Mouse antibody for Herpes simplex virus |
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115 | Virostat | 100 ug | EUR 386.26 |
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, type 2 nuclear for WB, ELISA. |
Mouse antibody for Herpes simplex virus |
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119 | Virostat | 100 ug | EUR 425.4 |
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, 1&2 nuclear for WB, ELISA. |
Human Herpes simplexvirus,HSV ELISA Kit |
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201-12-1986 | SunredBio | 96 tests | EUR 528 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Mouse antibody for Herpes simplex virus |
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190 | Virostat | 100 ug | EUR 386.26 |
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, 1&2 gD for WB, ELISA. |
Mouse antibody for Herpes simplex virus |
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192 | Virostat | 100 ug | EUR 386.26 |
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, 1&2 gD for WB, ELISA. |
Mouse antibody for Herpes simplex virus |
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194 | Virostat | 100 ug | EUR 386.26 |
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, 1&2 gD for WB, ELISA. |
Mouse antibody for Herpes simplex virus |
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195 | Virostat | 100 ug | EUR 425.4 |
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, 1&2 gD for WB, ELISA. |
Mouse antibody for Herpes simplex virus 1&2 |
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196 | Virostat | 100 ug | EUR 431.93 |
Description: This is Mouse monoclonal FITC conjugated antibody against Herpes simplex virus, 1&2 gD for WB, ELISA. |
Mouse antibody for Herpes simplex virus |
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197 | Virostat | 100 ug | EUR 425.4 |
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, 1&2 gD for WB, ELISA. |
Mouse antibody for Herpes simplex virus |
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198 | Virostat | 100 ug | EUR 386.26 |
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, 1&2 gD for WB, ELISA. |
Mouse antibody for Herpes simplex virus |
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199 | Virostat | 100 ug | EUR 386.26 |
Description: This is purified Mouse monoclonal antibody against Herpes simplex virus, 1&2 gD for WB, ELISA. |
Herpes Simplex Virus 2 (HSV-2) Antibody (FITC) |
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abx023037-1ml | Abbexa | 1 ml | EUR 678 |
Virus Herpes Simplex Virus 2 (HSV-2) Protein |
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abx670261-1ml | Abbexa | 1 ml | EUR 360 |
Recombinant Herpes Simplex Virus-1 gD |
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7-07126 | CHI Scientific | 100µg | Ask for price |
Recombinant Herpes Simplex Virus-1 gD |
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7-07127 | CHI Scientific | 500µg | Ask for price |
Recombinant Herpes Simplex Virus-1 gD |
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7-07128 | CHI Scientific | 1000µg | Ask for price |
Recombinant Herpes Simplex Virus-1 gG |
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7-07129 | CHI Scientific | 100µg | Ask for price |
Recombinant Herpes Simplex Virus-1 gG |
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7-07130 | CHI Scientific | 500µg | Ask for price |
Recombinant Herpes Simplex Virus-1 gG |
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7-07131 | CHI Scientific | 1000µg | Ask for price |
Recombinant Herpes Simplex Virus-2 gD |
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7-07132 | CHI Scientific | 100µg | Ask for price |
Recombinant Herpes Simplex Virus-2 gD |
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7-07133 | CHI Scientific | 500µg | Ask for price |
Recombinant Herpes Simplex Virus-2 gD |
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7-07134 | CHI Scientific | 1000µg | Ask for price |
Recombinant Herpes Simplex Virus-2 gG |
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7-07135 | CHI Scientific | 100µg | Ask for price |
Recombinant Herpes Simplex Virus-2 gG |
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7-07136 | CHI Scientific | 500µg | Ask for price |
Recombinant Herpes Simplex Virus-2 gG |
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7-07137 | CHI Scientific | 1000µg | Ask for price |