Translating the combination of gene therapy and tissue engineering for treating recessive dystrophic epidermolysis bullosa.

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Translating the combination of gene therapy and tissue engineering for treating recessive dystrophic epidermolysis bullosa.

The mix of gene remedy and tissue engineering is among the most promising methods for the therapy of recessive dystrophic epidermolysis bullosa (RDEB). RDEB is a uncommon genetic illness characterised by mutations within the COL7A1 gene, encoding sort VII collagen (COLVII), which kinds anchoring fibrils on the dermal-epidermal junction of the pores and skin. This illness causes extreme blistering and solely palliative remedies are supplied.

On this examine, the bottom of a technique combining gene remedy and a tissue-engineered pores and skin substitute (TES), which might be appropriate for the everlasting closure of pores and skin wounds, was set-up. As a excessive transduction effectivity into fibroblasts and/or keratinocytes appears to be a prerequisite for a sturdy and sustained correction of RDEB, completely different envelope pseudotyped retroviral vectors and the transduction enhancer EF-C have been examined. When inexperienced fluorescent protein (GFP) was used as a reporter gene to judge the retroviral-mediated gene switch, the fibroblast an infection effectivity was 30 % increased with the Ampho pseudotyped vector as in contrast with the opposite pseudotypes. A minimum of a 3.1-fold and a 1.3-fold elevated transduction have been obtained in fibroblasts and keratinocytes, respectively, with EF-C as in contrast with polybrene.

A steady and intense deposit of haemagglutinin (HA)-COLVII was noticed on the dermal-epidermal junction of self-assembled TESs fabricated from cells transduced with a HA-tagged COL7A1 vector. Moreover, HA-tagged basal epidermal cells expressing keratin 19 have been noticed in TESs, suggesting stem cell transduction. This method could possibly be a priceless therapeutic choice to additional develop, so as to enhance the long-term life high quality of RDEB sufferers.

ASSESSMENT OF AAV SEROTYPE TROPISM IN HUMAN RETINAL EXPLANTS.

Advances within the discovery of the causes of monogenic retinal issues, mixed with applied sciences for the supply of DNA to the retina, provide huge alternatives for the therapy of beforehand untreatable blinding illnesses. Nevertheless, for gene augmentation to be best, vectors which have the right cell-type specificity are wanted. Whereas animal fashions are very helpful, they typically exhibit variations in retinal cell floor receptors in comparison with the human retina. After 7 days in tradition, it was discovered that AAV2/four and AAV2/5 have been significantly environment friendly at transducing photoreceptor cells and that AAV2/5 was extremely particular to the outer nuclear layer, whereas AAV2/eight displayed persistently low transduction of photoreceptors. To validate the authenticity of the organotypic tradition system, the transduction of the identical set of AAVs was additionally in contrast in a pig mannequin, during which sub-retinal injections in vivo have been in comparison with cultured and transduced organotypic cultures ex vivo.
This examine exhibits how completely different AAV serotypes behave within the human retina and supplies perception for additional investigation of every of those serotypes for gene augmentation-based therapy of inherited retinal degeneration.  To know the operate of membrane proteins, it’s crucial to know their topology. For such research, a cut up inexperienced fluorescent protein (GFP) methodology is helpful. GFP is barrel-shaped, consisting of 11 β-sheets. When the primary ten β-sheets protein. Nevertheless, this may solely happen when the 2 domains co-localize in the identical mobile compartment. We now have developed an easy-to-use Gateway vector set for figuring out on which facet of the membrane the N- and C-termini are positioned.
Two vectors have been designed for making N- and C-terminal fusions between the membrane proteins-of-interest and vector set for the transmembrane area,of the ER membrane protein, calnexin, after transient expression in Nicotiana benthamiana leaves. We noticed GFP sign from the ER after we reciprocally co-expressed with cytosolic sign emission.
Translating the combination of gene therapy and tissue engineering for treating recessive dystrophic epidermolysis bullosa.

Translating the combination of gene therapy and tissue engineering for treating recessive dystrophic epidermolysis bullosa.

Regulatory elements and transcriptional control of chicken vasa homologue (CVH) promoter in chicken primordial germ cells.

BACKGROUND
Primordial germ cells (PGCs), the precursors of purposeful gametes, have distinct traits and exhibit a number of distinctive molecular mechanisms to take care of pluripotency and germness compared to somatic cells. They specific germ cell-specific RNA binding proteins (RBPs) by modulating tissue-specific cis- and trans-regulatory parts. Research on gene buildings of rooster vasa homologue (CVH), a rooster RNA binding protein, concerned in temporal and spatial regulation are thus essential not just for understanding the molecular mechanisms that regulate germ cell destiny, but in addition for sensible functions of primordial germ cells. Nevertheless, very restricted research can be found on regulatory parts that management germ cell-specific expression in rooster. Subsequently, we investigated the intricate regulatory mechanism(s) that governs transcriptional management of CVH.
RESULTS
We constructed inexperienced fluorescence protein (GFP) or luciferase reporter vectors containing the varied 5′ flanking areas of CVH gene. From the 5′ deletion and fragmented assays in rooster PGCs, we’ve recognized a CVH promoter that locates at -316 to +275 base pair fragment with the very best luciferase exercise. Moreover, we confirmed for the primary time that the 5′ untranslated area (UTR) containing intron 1 is required for promoter exercise of the CVH gene in rooster PGCs. Moreover, utilizing a transcription issue binding prediction, transcriptome evaluation and siRNA-mediated knockdown, we’ve recognized {that a} set of transcription components play a job within the PGC-specific CVH gene expression.

Ligation-Free Cloning System
E36E002 100 Reactions
EUR 414.29

Hybridoma Cloning Factor(Protein Free)
E28M-2002 2.5mg(For 500ml medium)
EUR 400

Pro Ligation-Free Cloning System
E36E086 20 Reactions
EUR 135.71

Pro Ligation-Free Cloning System
E36E087 50 Reactions
EUR 250

Mouse 5- formyltetrahydrofolate cyclo- ligase, Mthfs ELISA KIT
ELI-46511m 96 Tests
EUR 1038

Human 5- formyltetrahydrofolate cyclo- ligase, MTHFS ELISA KIT
ELI-15967h 96 Tests
EUR 988.8

Rabbit 5- formyltetrahydrofolate cyclo- ligase, MTHFS ELISA KIT
ELI-23229Ra 96 Tests
EUR 1113.6

T4 DNA Ligase (RNase-free)
K1100-100000 100KU
EUR 43
Description: T4 DNA ligase, fragments ligation for cohesive or blunt end, nicks repair

T4 DNA Ligase (RNase-free)
K1100-200000 200KU
EUR 60
Description: T4 DNA ligase, fragments ligation for cohesive or blunt end, nicks repair

T4 DNA Ligase (RNase-free)
K1100-40000 40KU
EUR 25
Description: T4 DNA ligase, fragments ligation for cohesive or blunt end, nicks repair

T1 Cloning Kit
20-abx098055
  • EUR 594.00
  • EUR 794.40
  • 20 rxns
  • 60 rxns

T3 Cloning Kit
20-abx098057
  • EUR 594.00
  • EUR 794.40
  • 20 rxns
  • 60 rxns

T4 RNA Ligase 1 (ssRNA Ligase)
M1218-100 each
EUR 307.2

T4 RNA Ligase 2 (dsRNA Ligase)
M1219-100 each
EUR 346.8

Blunt Cloning Kit
20-abx098059
  • EUR 594.00
  • EUR 794.40
  • 20 rxns
  • 60 rxns

Blunt3 Cloning Kit
20-abx098061
  • EUR 594.00
  • EUR 794.40
  • 20 rxns
  • 60 rxns

Human RNA 2',3'-Cyclic Phosphate And 5'-OH Ligase (RtcB) ELISA Kit
abx382984-96tests 96 tests
EUR 1093.2

DNA Ligase I (DNA Ligase I) Antibody
abx232431-100ug 100 ug
EUR 577.2

LIG1 cloning plasmid
CSB-CL012930HU-10ug 10ug
EUR 939.6
Description: A cloning plasmid for the LIG1 gene.

LIG3 cloning plasmid
CSB-CL012931HU1-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the LIG3 gene.

LIG3 cloning plasmid
CSB-CL012931HU2-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the LIG3 gene.

LIG4 cloning plasmid
CSB-CL012932HU-10ug 10ug
EUR 669.6
Description: A cloning plasmid for the LIG4 gene.

T5 Zero Cloning Kit
20-abx098058
  • EUR 627.60
  • EUR 878.40
  • 20 rxns
  • 60 rxns

T1 Simple Cloning Kit
20-abx098056
  • EUR 594.00
  • EUR 794.40
  • 20 rxns
  • 60 rxns

Cyclopamine, Free Base
27013 10 mg
EUR 130
Description: Cyclopamine inhibits activation of the Hedgehog response pathway by binding to and inhibiting the proto-oncogene Smoothened (SMO). The effects of oncogenic mutations that either activate SMO or inactivate the tumor suppressor Patched (Ptch) can be reversed by cyclopamine treatment. Cyclopamine has also been shown to induce apoptosis in colorectal adenoma cell lines, carcinoma-derived cell lines, and skin basal cell carcinomas and is being investigated as an anti-cancer drug.

Cyclopamine, Free Base
ADC-P-079 unit Ask for price

Blunt Zero Cloning Kit
20-abx098062
  • EUR 627.60
  • EUR 878.40
  • 20 rxns
  • 60 rxns

T4 Ligase
C301-01 40,000 U
EUR 136.8

Blunt Simple Cloning Kit
20-abx098060
  • EUR 594.00
  • EUR 794.40
  • 20 rxns
  • 60 rxns

CYCS cloning plasmid
CSB-CL006328HU1-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the CYCS gene.

CYCS cloning plasmid
CSB-CL006328HU2-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the CYCS gene.

Hyper Assembly Cloning Kit
K1054-100 100 rxns
EUR 250
Description: used for fast, directional cloning of one or more fragments of DNA into any vector.

Hyper Assembly Cloning Kit
K1054-25 25 rxns
EUR 80
Description: used for fast, directional cloning of one or more fragments of DNA into any vector.

Hyper Assembly Cloning Kit
K1054-50 50 rxns
EUR 150
Description: used for fast, directional cloning of one or more fragments of DNA into any vector.

T4 DNA Ligase (40000U)
9K-005-0002 40000U
EUR 351.28

E. coli NAD+-dependent DNA ligase assay kit Plus (E. coli ligase included)
NLA100KE 100 assays
EUR 444.91
Description: This product includes 800 ul of 10 x buffer, 44 ul of 100x DNA substrate, 44 ul of 100 x NAD+, 21 ml of Reagent T, 10 ul of 500 x E. coli ligase and 220 ul of 10 x fluorescence dye for 100 assays.

RNA 2',3'-Cyclic Phosphate And 5'-OH Ligase (RTCB) Antibody
20-abx317901
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

RNA 2',3'-Cyclic Phosphate And 5'-OH Ligase (RTCB) Antibody
20-abx126502
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Cloning
S201 - Ask for price

T4 DNA Ligase
abx098136-10kU 10 kU
EUR 385.2

T4 DNA Ligase
abx098136-20Ku 20 Ku
EUR 477.6

T4 DNA ligase
402-002 400 WEISS UNITS
EUR 75.6

T4 DNA ligase
402-010 2000 WEISS UNITS
EUR 184.8

DNA Ligase IV
E8ET1705-77 100ul
EUR 275
Description: Available in various conjugation types.

DNA Ligase I
E8ET7107-14 100ul
EUR 275
Description: Available in various conjugation types.

T4 DNA Ligase
RK21501 80000U
EUR 10

DNA Ligase I ELISA KIT|Human
EF009143 96 Tests
EUR 826.8

Taq DNA Ligase
RK20502 2000U
EUR 105

RNA 2',3'-Cyclic Phosphate And 5'-OH Ligase (RTCB) Antibody (HRP)
20-abx314719
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

E.coli DNA Ligase
RK20501 1000U
EUR 20.5

T&A Cloning Kit + ECOS 101DH5a
FYC005-20P 1 vial Ask for price

RNA 2',3'-Cyclic Phosphate And 5'-OH Ligase (RTCB) Antibody (FITC)
20-abx314720
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Ultra-Universal TOPO Cloning Kit
C603-01 20 rxns (10 μll/rxn)
EUR 78.4

Ultra-Universal TOPO Cloning Kit
C603-02 40 rxns (10 μll/rxn)
EUR 139.2

ig-Fusion Cloning Kit - 100 Reactions
4117 1/EA
EUR 1600.8

5 min TOPO-Blunt Cloning Kit
C602-01 20 rxns (2 µl/rxn)
EUR 65.41

5 min TOPO-Blunt Cloning Kit
C602-02 40 rxns (2 µl/rxn)
EUR 116

ClonExpress II One Step Cloning Kit
C112-01 25 rxn
EUR 202.8

ClonExpress II One Step Cloning Kit
C112-02 50 rxn
EUR 259.2

Rat DNA Ligase 1 (LIG1) ELISA Kit
abx256672-96tests 96 tests
EUR 886.8

Rat Lig1(DNA ligase 1) ELISA Kit
ER0697 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 46.9pg/ml

Rat Lig1/ DNA ligase 1 ELISA Kit
E0564Ra 1 Kit
EUR 775.2

5min TA/Blunt-Zero Cloning Kit
C601-01 25 rxn
EUR 253.2

5min TA/Blunt-Zero Cloning Kit
C601-02 50 rxn
EUR 360

ELISA kit for Rat DNA ligase 1
EK4909 96 tests
EUR 804
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat DNA ligase 1 in samples from serum, plasma, tissue homogenates and other biological fluids.

RNA 2',3'-Cyclic Phosphate And 5'-OH Ligase (RTCB) Antibody (Biotin)
20-abx314721
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

PrecisionX Multiplex gRNA Cloning Kit
CAS9-GRNA-KIT 10 rxn
EUR 387

DNA ligase Antibody
20-abx110554
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

E3 ubiquitin ligase
E8ER1907-88 100ul
EUR 275
Description: Available in various conjugation types.

Human DNA Ligase 4 (LIG4) ELISA Kit
abx388272-96tests 96 tests
EUR 1093.2

Human DNA Ligase 1 (LIG1) ELISA Kit
abx520993-96tests 96 tests
EUR 886.8

Mouse DNA Ligase 1 (LIG1) ELISA Kit
abx520994-96tests 96 tests
EUR 886.8

Mouse Lig1/ DNA ligase 1 ELISA Kit
E0869Mo 1 Kit
EUR 758.4

Mouse Lig3/ DNA ligase 3 ELISA Kit
E0870Mo 1 Kit
EUR 758.4

Human DNA ligase 4, LIG4 ELISA KIT
ELI-47684h 96 Tests
EUR 988.8

Human LIG3(DNA ligase 3) ELISA Kit
EH1715 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

Mouse DNA ligase 3, Lig3 ELISA KIT
ELI-09214m 96 Tests
EUR 1038

Human DNA ligase 1, LIG1 ELISA KIT
ELI-09438h 96 Tests
EUR 988.8

Human DNA ligase 3, LIG3 ELISA KIT
ELI-09439h 96 Tests
EUR 988.8

Human LIG1/ DNA ligase 1 ELISA Kit
E1464Hu 1 Kit
EUR 726

Human LIG3/ DNA ligase 3 ELISA Kit
E1465Hu 1 Kit
EUR 726

Mouse DNA ligase 4, Lig4 ELISA KIT
ELI-32012m 96 Tests
EUR 1038

Mouse DNA ligase 1, Lig1 ELISA KIT
ELI-32823m 96 Tests
EUR 1038

Mouse DNA ligase 1 (LIG1) ELISA Kit
RK13017 96T
EUR 280

Human DNA ligase 3 (LIG3) ELISA Kit
RK10591 96T
EUR 280

Human DNA ligase 1 (LIG1) ELISA Kit
RK10630 96T
EUR 280

pUCM-T Cloning Vector Kit
BS435 20Preps
EUR 112.2

pUCM-T Cloning Vector Kit
BS436 100preps
EUR 268.8

T&A Cloning Kits
FYC001-20P 1 vial Ask for price

ClonExpress Ultra One Step Cloning Kit
C115-01 25 rxn
EUR 231.6

ClonExpress Ultra One Step Cloning Kit
C115-02 50 rxn
EUR 301.2

Quick-Stick Ligase
BIO-27027 50 Reactions Ask for price

Quick-Stick Ligase
BIO-27028 100 Reactions Ask for price

ClonExpress MultiS One Step Cloning Kit
C113-01 10 rxn
EUR 192

ClonExpress MultiS One Step Cloning Kit
C113-02 25 rxn
EUR 268.8

E3 ligase Ligand 8
HY-43961 100mg
EUR 817.2

ELISA kit for Human DNA ligase 3
EK3567 96 tests
EUR 804
Description: Enzyme-linked immunosorbent assay kit for quantification of Human DNA ligase 3 in samples from serum, plasma, tissue homogenates and other biological fluids.

Taq DNA Ligase - 10,000 units
3219 1/EA
EUR 303.6

Chicken DNA ligase 4, LIG4 ELISA KIT
ELI-47798c 96 Tests
EUR 1113.6

ig-Fusion Cloning Kit - 10 Reactions
4111 1/EA
EUR 315.6

T&A Cloning Kit + ECOS 9-5JM109
FYC006-20P 1 vial Ask for price

T4 RNA Ligase, 10-20u/ul
BEL0021 1KU
EUR 202.68

Express Cloning Checker Kit I
K5011200 200 reactions
EUR 96

Express Cloning Checker Kit II
K5012100 100 reactions
EUR 96

E. coli DNA Ligase
M1217-100 each
EUR 385.2

gRNA- Cloning
PVT10957 2 ug
EUR 361.2

Seamless cloning Master Mix (Kit)
B632219 40RXN, 40prep
EUR 319.96

Express Cloning Checker Kit III
K5013200 200 reactions
EUR 134

Lig1 ELISA Kit| Rat DNA ligase 1 ELISA Kit
EF017510 96 Tests
EUR 826.8

T4 DNA Ligase (Rapid)
N103-01 600,000 U
EUR 675.6

pUC57- T4 DNA Ligase
PVT10516 2 ug
EUR 319.2

T4 DNA Ligase Buffer
9102-1 each
EUR 105.6

anti-DNA Ligase I
YF-PA24084 50 ul
EUR 400.8
Description: Mouse polyclonal to DNA Ligase I

anti-DNA Ligase I
YF-PA12953 50 ul
EUR 435.6
Description: Mouse polyclonal to DNA Ligase I

Lig1 ELISA Kit| Mouse DNA ligase 1 ELISA Kit
EF015382 96 Tests
EUR 826.8

Lig3 ELISA Kit| Mouse DNA ligase 3 ELISA Kit
EF015383 96 Tests
EUR 826.8

T4 DNA Ligase protein
30R-2898 250 units
EUR 268.8
Description: Purified recombinant Human T4 DNA Ligase protein

T4 DNA Ligase Plasmid
PVT7008 2 ug
EUR 614.4

anti-DNA Ligase III
YF-PA24085 50 ul
EUR 400.8
Description: Mouse polyclonal to DNA Ligase III

Cyclopamine, Free Base [CAS# 4449-51-8]
C-8700 10 mg
EUR 55

DNA Ligase 1 Antibody
20-abx141113
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

DNA Ligase 3 Antibody
20-abx141114
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

DNA ligase 3 antibody
22508-100ul 100ul
EUR 468

DNA ligase 3 antibody
22940-100ul 100ul
EUR 468

DNA ligase 3 antibody
70R-12710 100 ul
EUR 548.4
Description: Affinity purified Rabbit polyclonal DNA ligase 3 antibody

DNA ligase 3 antibody
70R-12712 100 ul
EUR 548.4
Description: Affinity purified Rabbit polyclonal DNA ligase 3 antibody

DNA Ligase 4 antibody
70R-50002 100 ul
EUR 292.8
Description: Purified Polyclonal DNA Ligase 4 antibody

DNA Ligase 4 antibody
70R-35788 100 ug
EUR 392.4
Description: Rabbit polyclonal DNA Ligase 4 antibody

DNA ligase IV Antibody
35335-100ul 100ul
EUR 468

DNA Ligase IV Antibody
49667-100ul 100ul
EUR 399.6

DNA Ligase IV Antibody
49667-50ul 50ul
EUR 286.8

DNA Ligase I Antibody
49797-100ul 100ul
EUR 399.6

DNA Ligase I Antibody
49797-50ul 50ul
EUR 286.8

DNA Ligase IV Antibody
DF6588 100ul
EUR 280
Description: Human,Mouse,Rat

DNA Ligase IV Antibody
DF3613-100ul 100ul
EUR 280

DNA Ligase IV Antibody
DF3613-200ul 200ul
EUR 350

DNA Ligase IV Antibody
DF6588-100ul 100ul
EUR 280

DNA Ligase IV Antibody
DF6588-200ul 200ul
EUR 350

DNA Ligase IV Antibody
DF3613 100ul
EUR 280
Description: Human

KITLG cloning plasmid
CSB-CL012376HU-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the KITLG gene.

T4 DNA Ligase, 5u/ul
B1122 1KU
EUR 190.5

T4 DNA Ligase, 5u/ul
B1125 200U
EUR 93.41

Rat Biotin protein ligase(HLCS) ELISA kit
E02B0920-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Biotin protein ligase(HLCS) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Biotin protein ligase(HLCS) ELISA kit
E02B0920-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Biotin protein ligase(HLCS) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Biotin protein ligase(HLCS) ELISA kit
E02B0920-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Biotin protein ligase(HLCS) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Biotin protein ligase(HLCS) ELISA kit
E08B0920-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine Biotin protein ligase(HLCS) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Biotin protein ligase(HLCS) ELISA kit
E08B0920-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine Biotin protein ligase(HLCS) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Biotin protein ligase(HLCS) ELISA kit
E08B0920-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine Biotin protein ligase(HLCS) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Biotin protein ligase(HLCS) ELISA kit
E07B0920-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine Biotin protein ligase(HLCS) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Biotin protein ligase(HLCS) ELISA kit
E07B0920-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine Biotin protein ligase(HLCS) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Biotin protein ligase(HLCS) ELISA kit
E07B0920-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine Biotin protein ligase(HLCS) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

DNA ligase Antibody (HRP)
20-abx109115
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

DNA Ligase III Antibody
DF6677 100ul
EUR 280
Description: Human,Mouse,Rat

DNA Ligase III Antibody
DF6677-100ul 100ul
EUR 280

DNA Ligase III Antibody
DF6677-200ul 200ul
EUR 350

Rat E3/ubiquitin protein ligase ELISA kit
E02E0118-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat E3/ubiquitin protein ligase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat E3/ubiquitin protein ligase ELISA kit
E02E0118-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat E3/ubiquitin protein ligase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat E3/ubiquitin protein ligase ELISA kit
E02E0118-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat E3/ubiquitin protein ligase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog E3/ubiquitin protein ligase ELISA kit
E08E0118-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine E3/ubiquitin protein ligase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog E3/ubiquitin protein ligase ELISA kit
E08E0118-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine E3/ubiquitin protein ligase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog E3/ubiquitin protein ligase ELISA kit
E08E0118-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine E3/ubiquitin protein ligase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig E3/ubiquitin protein ligase ELISA kit
E07E0118-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine E3/ubiquitin protein ligase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig E3/ubiquitin protein ligase ELISA kit
E07E0118-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine E3/ubiquitin protein ligase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig E3/ubiquitin protein ligase ELISA kit
E07E0118-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine E3/ubiquitin protein ligase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
CONCLUSIONS
These outcomes show that cis-elements and transcription components localizing within the 5′ flanking area together with the 5′ UTR and an intron are essential for transcriptional regulation of the CVH gene in rooster PGCs. Lastly, this data will contribute to analysis research in areas of reproductive biology, establishing of germ cell-specific artificial promoter for tracing primordial germ cells in addition to understanding the transcriptional regulation for sustaining germness in PGCs.

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