Translating the combination of gene therapy and tissue engineering for treating recessive dystrophic epidermolysis bullosa.

Translating the combination of gene therapy and tissue engineering for treating recessive dystrophic epidermolysis bullosa.

The mix of gene remedy and tissue engineering is among the most promising methods for the therapy of recessive dystrophic epidermolysis bullosa (RDEB). RDEB is a uncommon genetic illness characterised by mutations within the COL7A1 gene, encoding sort VII collagen (COLVII), which kinds anchoring fibrils on the dermal-epidermal junction of the pores and skin. This illness causes extreme blistering and solely palliative remedies are supplied.

On this examine, the bottom of a technique combining gene remedy and a tissue-engineered pores and skin substitute (TES), which might be appropriate for the everlasting closure of pores and skin wounds, was set-up. As a excessive transduction effectivity into fibroblasts and/or keratinocytes appears to be a prerequisite for a sturdy and sustained correction of RDEB, completely different envelope pseudotyped retroviral vectors and the transduction enhancer EF-C have been examined. When inexperienced fluorescent protein (GFP) was used as a reporter gene to judge the retroviral-mediated gene switch, the fibroblast an infection effectivity was 30 % increased with the Ampho pseudotyped vector as in contrast with the opposite pseudotypes. A minimum of a 3.1-fold and a 1.3-fold elevated transduction have been obtained in fibroblasts and keratinocytes, respectively, with EF-C as in contrast with polybrene.

A steady and intense deposit of haemagglutinin (HA)-COLVII was noticed on the dermal-epidermal junction of self-assembled TESs fabricated from cells transduced with a HA-tagged COL7A1 vector. Moreover, HA-tagged basal epidermal cells expressing keratin 19 have been noticed in TESs, suggesting stem cell transduction. This method could possibly be a priceless therapeutic choice to additional develop, so as to enhance the long-term life high quality of RDEB sufferers.

ASSESSMENT OF AAV SEROTYPE TROPISM IN HUMAN RETINAL EXPLANTS.

Advances within the discovery of the causes of monogenic retinal issues, mixed with applied sciences for the supply of DNA to the retina, provide huge alternatives for the therapy of beforehand untreatable blinding illnesses. Nevertheless, for gene augmentation to be best, vectors which have the right cell-type specificity are wanted. Whereas animal fashions are very helpful, they typically exhibit variations in retinal cell floor receptors in comparison with the human retina. After 7 days in tradition, it was discovered that AAV2/four and AAV2/5 have been significantly environment friendly at transducing photoreceptor cells and that AAV2/5 was extremely particular to the outer nuclear layer, whereas AAV2/eight displayed persistently low transduction of photoreceptors. To validate the authenticity of the organotypic tradition system, the transduction of the identical set of AAVs was additionally in contrast in a pig mannequin, during which sub-retinal injections in vivo have been in comparison with cultured and transduced organotypic cultures ex vivo.
This examine exhibits how completely different AAV serotypes behave within the human retina and supplies perception for additional investigation of every of those serotypes for gene augmentation-based therapy of inherited retinal degeneration.  To know the operate of membrane proteins, it’s crucial to know their topology. For such research, a cut up inexperienced fluorescent protein (GFP) methodology is helpful. GFP is barrel-shaped, consisting of 11 β-sheets. When the primary ten β-sheets protein. Nevertheless, this may solely happen when the 2 domains co-localize in the identical mobile compartment. We now have developed an easy-to-use Gateway vector set for figuring out on which facet of the membrane the N- and C-termini are positioned.
Two vectors have been designed for making N- and C-terminal fusions between the membrane proteins-of-interest and vector set for the transmembrane area,of the ER membrane protein, calnexin, after transient expression in Nicotiana benthamiana leaves. We noticed GFP sign from the ER after we reciprocally co-expressed with cytosolic sign emission.
 Translating the combination of gene therapy and tissue engineering for treating recessive dystrophic epidermolysis bullosa.

Translating the combination of gene therapy and tissue engineering for treating recessive dystrophic epidermolysis bullosa.

Regulatory elements and transcriptional control of chicken vasa homologue (CVH) promoter in chicken primordial germ cells.

BACKGROUND
Primordial germ cells (PGCs), the precursors of purposeful gametes, have distinct traits and exhibit a number of distinctive molecular mechanisms to take care of pluripotency and germness compared to somatic cells. They specific germ cell-specific RNA binding proteins (RBPs) by modulating tissue-specific cis- and trans-regulatory parts. Research on gene buildings of rooster vasa homologue (CVH), a rooster RNA binding protein, concerned in temporal and spatial regulation are thus essential not just for understanding the molecular mechanisms that regulate germ cell destiny, but in addition for sensible functions of primordial germ cells. Nevertheless, very restricted research can be found on regulatory parts that management germ cell-specific expression in rooster. Subsequently, we investigated the intricate regulatory mechanism(s) that governs transcriptional management of CVH.
RESULTS
We constructed inexperienced fluorescence protein (GFP) or luciferase reporter vectors containing the varied 5′ flanking areas of CVH gene. From the 5′ deletion and fragmented assays in rooster PGCs, we’ve recognized a CVH promoter that locates at -316 to +275 base pair fragment with the very best luciferase exercise. Moreover, we confirmed for the primary time that the 5′ untranslated area (UTR) containing intron 1 is required for promoter exercise of the CVH gene in rooster PGCs. Moreover, utilizing a transcription issue binding prediction, transcriptome evaluation and siRNA-mediated knockdown, we’ve recognized {that a} set of transcription components play a job within the PGC-specific CVH gene expression.

Human Free PSA (f-PSA) ELISA Kit

PRB-5049-FREE-5 5 x 96 assays
EUR 2739.6

PrecisionX Multiplex gRNA Cloning Kit

CAS9-GRNA-KIT 10 rxn
EUR 534

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 183.6

Frit Kit

FRIT-KIT 1each
EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit

PACK-KIT 1pack
EUR 1242
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 319.2

AXYPET STARTER KIT 1 AP-20, AP-200 & AP-1000 WITH ADDITIONAL FREE RACKS OF AXYGEN PIPETTE TIPS

AP-STR-KIT-1 1/pk
EUR 426
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET STARTER KIT 2 AP-10, AP-100 & AP-1000 WITH ADDITIONAL FREE RACKS OF AXYGEN PIPETTE TIPS

AP-STR-KIT-2 1/pk
EUR 440.4
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1332

Pesticide Kit Containing All 10 Multi-Compound Mixes

SPXPR-KIT 1ML
EUR 1923.18

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1358.4

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1358.4

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1358.4

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 966

Free-T3/ Rat Free- T3 ELISA Kit

ELA-E0450r 96 Tests
EUR 1063.2

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
EUR 1358.4

Claritas PPT Grade Minerals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXMT-KIT 30ML
EUR 237.12

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 5929.2

Claritas PPT Grade Heavy Metals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXHM-KIT 30ML
EUR 245.1

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 5929.2

Human Free Tri-iodothyronine, Free-T3 ELISA Kit

CSB-E05075h-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human Free Tri-iodothyronine, Free-T3 in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Free Tri-iodothyronine, Free-T3 ELISA Kit

1-CSB-E05075h
  • EUR 600.00
  • EUR 4082.40
  • EUR 2184.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Free Tri-iodothyronine, Free-T3 in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Free Tri-iodothyronine, Free-T3 ELISA Kit

CSB-E05076r-24T 1 plate of 24 wells
EUR 198
Description: Quantitative competitive ELISA kit for measuring Rat Free Tri-iodothyronine, Free-T3 in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Free Tri-iodothyronine, Free-T3 ELISA Kit

1-CSB-E05076r
  • EUR 600.00
  • EUR 4082.40
  • EUR 2184.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitative competitive ELISA kit for measuring Rat Free Tri-iodothyronine, Free-T3 in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse Free Tri-iodothyronine, Free-T3 ELISA Kit

CSB-E05077m-24T 1 plate of 24 wells
EUR 198
Description: Quantitative competitive ELISA kit for measuring Mouse Free Tri-iodothyronine, Free-T3 in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse Free Tri-iodothyronine, Free-T3 ELISA Kit

1-CSB-E05077m
  • EUR 600.00
  • EUR 4082.40
  • EUR 2184.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitative competitive ELISA kit for measuring Mouse Free Tri-iodothyronine, Free-T3 in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Canine Free Tri-iodothyronine(Free-T3) ELISA Kit

CSB-E13195c-24T 1 plate of 24 wells
EUR 198
Description: Quantitative competitive ELISA kit for measuring Canine Free Tri-iodothyronine (Free-T3) in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Canine Free Tri-iodothyronine(Free-T3) ELISA Kit

1-CSB-E13195c
  • EUR 600.00
  • EUR 4082.40
  • EUR 2184.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitative competitive ELISA kit for measuring Canine Free Tri-iodothyronine(Free-T3) in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
EUR 2558.4

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT 1 kit
EUR 2558.4

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
EUR 2558.4

Estriol free ELISA kit

55R-IB79123 96 wells
EUR 596.4
Description: ELISA kit for the detection of Estriol free in the research laboratory

hCG free ELISA Kit

55R-IB79127 96 wells
EUR 912
Description: ELISA kit for the detection of hCG in the research laboratory

Free Glycerol Assay Kit

55R-1472 100 assays
EUR 867.6
Description: Assay Kit for detection of Free Glycerol in the research laboratory

Free Estriol ELISA Kit

abx257154-96tests 96 tests
EUR 764.4

MQ ORGANIC FREE KIT

CF0F01205 EACH
EUR 1106.94

MQ ION FREE KIT

CFIF01205 EACH
EUR 1210.68

IPTG, Animal-Free (Dioxane-Free)

1649-1G
EUR 138

IPTG, Animal-Free (Dioxane-Free)

1649-50G
EUR 1182

IPTG, Animal-Free (Dioxane-Free)

1649-5G
EUR 261.6

vWF Acty. Kit

ABP-ACT-KIT 12 x 8 microwells
EUR 513.6

vWF Ant. Kit

ABP-TOT-KIT 12 x 8 microwells
EUR 472.8

Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit

CN-02860M1 96T
EUR 571.2

Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit

CN-02860M2 48T
EUR 391.2

Human Free Tri-iodothyronine Indes,Free-T3 ELISA Kit

CN-03942H1 96T
EUR 586.8

Human Free Tri-iodothyronine Indes,Free-T3 ELISA Kit

CN-03942H2 48T
EUR 405.6

Rat Free Tri-iodothyronine Indes,Free-T3 ELISA Kit

CN-01991R1 96T
EUR 577.2

Rat Free Tri-iodothyronine Indes,Free-T4 ELISA Kit

CN-01991R2 48T
EUR 398.4

Human Free Tri-iodothyronine Indes(Free-T3)ELISA Kit

GA-E0060HM-48T 48T
EUR 346.8

Human Free Tri-iodothyronine Indes(Free-T3)ELISA Kit

GA-E0060HM-96T 96T
EUR 559.2

Mouse Free Tri-iodothyronine Indes(Free-T3)ELISA Kit

GA-E0078MS-48T 48T
EUR 403.2

Mouse Free Tri-iodothyronine Indes(Free-T3)ELISA Kit

GA-E0078MS-96T 96T
EUR 640.8

Human Free Tri-iodothyronine Indes,Free-T3 ELISA Kit

201-12-0013 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Rat Free Tri-iodothyronine Indes(Free-T3)ELISA Kit

GA-E0745RT-48T 48T
EUR 380.4

Rat Free Tri-iodothyronine Indes(Free-T3)ELISA Kit

GA-E0745RT-96T 96T
EUR 595.2

Rat Free Tri-iodothyronine Indes(Free-T3)ELISA Kit

QY-E10907 96T
EUR 433.2

Mouse Free Tri-iodothyronine Indes(Free-T3)ELISA Kit

QY-E20615 96T
EUR 433.2

Human Free Tri-iodothyronine Indes(Free-T3)ELISA Kit

QY-E00403 96T
EUR 433.2

T4 RNA Ligase 1 (ssRNA Ligase)

M1218-100
EUR 307.2

T4 RNA Ligase 2 (dsRNA Ligase)

M1219-100
EUR 346.8

T4 Ligase

C301-01 40,000 U
EUR 136.8

hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9)

CAS620A-KIT 1 kit
EUR 2582.4
Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression)

PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN300A-KIT 1 Kit
EUR 3357.6

PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN400A-KIT 1 Kit
EUR 3357.6

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN410A-KIT 1 Kit
EUR 5202

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN412A-KIT 1 Kit
EUR 5202

Filtration System 47mm Complete Assembly

5809/KIT EACH
EUR 427.69

Block-Free ELISA Reagent (Protein-Free)

M4101-250
EUR 548.4

Block-Free ELISA Reagent (Protein-Free)

M4101-50
EUR 183.6

ELISA kit for Canine Free Tri-iodothyronine Indes (Free-T3)

EK0028 96 tests
EUR 723.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Canine Free Tri-iodothyronine Indes (Free-T3) in samples from serum, plasma, tissue homogenates and other biological fluids.

ELISA kit for Rat Free Tri-iodothyronine Indes,Free-T3

EK0414 96 tests
EUR 723.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Free Tri-iodothyronine Indes,Free-T3 in samples from serum, plasma, tissue homogenates and other biological fluids.

mRNAExpress mRNA Synthesis kit (5 reactions)

MR-KIT-1 5 reactions
EUR 1382.4

42 Component Terpene Kit in Methanol

CAN-TERP-KIT 1ML
EUR 287.28

DNA Ligase I (DNA Ligase I) Antibody

abx232431-100ug 100 ug
EUR 577.2

Canadian Pesticide Kit Containing All 6 Mixes

CAN-CAN-KIT 1ML
EUR 758.1

AxioCam Erc5s rev 2, w/Accessory Kit

ZV-Erc5sAC-Kit 1 KIT
EUR 1611
Description: AxioCam Erc5s rev 2, w/Accessory Kit- connection cable Y-USB 2.0.- HDMI cable to DVI-D- SD card- IR remote control

Free Triiodothyronine (fT3) ELISA Kit

DLR-fT3-Ge-48T 48T
EUR 562.8
Description: A competitive inhibition quantitative ELISA assay kit for detection of Free Triiodothyronine (fT3) in samples from serum, plasma or other biological fluids.

Free Triiodothyronine (fT3) ELISA Kit

DLR-fT3-Ge-96T 96T
EUR 729.6
Description: A competitive inhibition quantitative ELISA assay kit for detection of Free Triiodothyronine (fT3) in samples from serum, plasma or other biological fluids.

Free Thyroxine (fT4) ELISA Kit

DLR-fT4-Ge-48T 48T
EUR 562.8
Description: A competitive inhibition quantitative ELISA assay kit for detection of Free Thyroxine (fT4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Free Thyroxine (fT4) ELISA Kit

DLR-fT4-Ge-96T 96T
EUR 729.6
Description: A competitive inhibition quantitative ELISA assay kit for detection of Free Thyroxine (fT4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Glucose,Free ELISA kit

E01G0030-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Glucose,Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Glucose,Free ELISA kit

E01G0030-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Glucose,Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Glucose,Free ELISA kit

E01G0030-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Glucose,Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Free cholesterol ELISA kit

E01F0212-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Free cholesterol ELISA kit

E01F0212-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Free cholesterol ELISA kit

E01F0212-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Free haemoglobin ELISA kit

E01F0223-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human Free haemoglobin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Free haemoglobin ELISA kit

E01F0223-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Human Free haemoglobin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Free haemoglobin ELISA kit

E01F0223-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Human Free haemoglobin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Free calcium ELISA kit

E01F0272-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Free calcium in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Free calcium ELISA kit

E01F0272-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Free calcium in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Free calcium ELISA kit

E01F0272-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Free calcium in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Testosterone, Free ELISA kit

E01T0561-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Testosterone, Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Testosterone, Free ELISA kit

E01T0561-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Testosterone, Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Testosterone, Free ELISA kit

E01T0561-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Testosterone, Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Salivary Free Estriol ELISA Kit

DEIA-LL101 96T
EUR 850.8
Description: Competitive immunoenzymatic colorimetric method for quantitative measurement of Estriol concentration in saliva.

Human Free Testosterone ELISA Kit

DEIA1723 96T
EUR 842.4
Description: Competitive immunoenzymatic colorimetric method for measurement of Free Testosterone concentration in human serum or plasma.

Free Thyroxine (fT4) ELISA Kit

DEIA1892 192T
EUR 847.2
Description: For Quantitative Determination of Free Thyroxine Concentration in Human Serum by a Microplate Enzyme Immunoassay.

Human Free Estriol ELISA Kit

DEIA2254 96T
EUR 445.2
Description: The Human Free Estriol ELISA Kit is an enzyme immunoassay for the quantitative measurement of free estriol (unconjugated estriol) in serum during the second half of pregnancy.

Rabbit Testosterone, Free ELISA kit

E04T0561-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Testosterone, Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Testosterone, Free ELISA kit

E04T0561-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Testosterone, Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Testosterone, Free ELISA kit

E04T0561-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Testosterone, Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Free cholesterol ELISA kit

E06F0203-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Free cholesterol ELISA kit

E06F0203-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Free cholesterol ELISA kit

E06F0203-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Free haemoglobin ELISA kit

E06F0222-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat Free haemoglobin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Free haemoglobin ELISA kit

E06F0222-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat Free haemoglobin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Free haemoglobin ELISA kit

E06F0222-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat Free haemoglobin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Free calcium ELISA kit

E06F0272-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Free calcium in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Free calcium ELISA kit

E06F0272-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Free calcium in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Free calcium ELISA kit

E06F0272-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Free calcium in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Glucose,Free ELISA kit

E06G0030-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Glucose,Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Glucose,Free ELISA kit

E06G0030-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Glucose,Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Glucose,Free ELISA kit

E06G0030-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Glucose,Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Free cholesterol ELISA kit

E02F0203-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Free cholesterol ELISA kit

E02F0203-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Free cholesterol ELISA kit

E02F0203-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Free haemoglobin ELISA kit

E02F0222-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Free haemoglobin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Free haemoglobin ELISA kit

E02F0222-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Free haemoglobin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Free haemoglobin ELISA kit

E02F0222-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Free haemoglobin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Free calcium ELISA kit

E02F0272-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Free calcium in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Free calcium ELISA kit

E02F0272-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Free calcium in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Free calcium ELISA kit

E02F0272-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Free calcium in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Glucose,Free ELISA kit

E02G0030-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Glucose,Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Glucose,Free ELISA kit

E02G0030-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Glucose,Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Glucose,Free ELISA kit

E02G0030-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Glucose,Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Free cholesterol ELISA kit

E04F0203-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Free cholesterol ELISA kit

E04F0203-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Free cholesterol ELISA kit

E04F0203-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Free haemoglobin ELISA kit

E04F0222-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rabbit Free haemoglobin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Free haemoglobin ELISA kit

E04F0222-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rabbit Free haemoglobin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Free haemoglobin ELISA kit

E04F0222-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rabbit Free haemoglobin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Free calcium ELISA kit

E04F0272-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Free calcium in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Free calcium ELISA kit

E04F0272-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Free calcium in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Free calcium ELISA kit

E04F0272-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Free calcium in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Glucose,Free ELISA kit

E04G0030-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Glucose,Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Glucose,Free ELISA kit

E04G0030-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Glucose,Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Glucose,Free ELISA kit

E04G0030-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Glucose,Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Testosterone, Free ELISA kit

E06T0561-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Testosterone, Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Testosterone, Free ELISA kit

E06T0561-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Testosterone, Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Testosterone, Free ELISA kit

E06T0561-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Testosterone, Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Free cholesterol ELISA kit

E08F0212-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Free cholesterol ELISA kit

E08F0212-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Free cholesterol ELISA kit

E08F0212-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Free cholesterol in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Free haemoglobin ELISA kit

E08F0223-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine Free haemoglobin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Free haemoglobin ELISA kit

E08F0223-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine Free haemoglobin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Free haemoglobin ELISA kit

E08F0223-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine Free haemoglobin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Free calcium ELISA kit

E08F0272-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Free calcium in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Free calcium ELISA kit

E08F0272-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Free calcium in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Free calcium ELISA kit

E08F0272-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Free calcium in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Glucose,Free ELISA kit

E08G0030-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Glucose,Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Glucose,Free ELISA kit

E08G0030-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Glucose,Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Glucose,Free ELISA kit

E08G0030-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Glucose,Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Testosterone, Free ELISA kit

E08T0561-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Testosterone, Free in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
CONCLUSIONS
These outcomes show that cis-elements and transcription components localizing within the 5′ flanking area together with the 5′ UTR and an intron are essential for transcriptional regulation of the CVH gene in rooster PGCs. Lastly, this data will contribute to analysis research in areas of reproductive biology, establishing of germ cell-specific artificial promoter for tracing primordial germ cells in addition to understanding the transcriptional regulation for sustaining germness in PGCs.

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