The elevated utilization of high-throughput applied sciences in most cancers analysis, together with genetic and drug screens, generates massive units of candidate targets that should be functionally validated for his or her roles in tumor growth. Thus, dependable and strong in vivo mannequin methods are wanted to carry out reverse genetic experiments. Ideally, these fashions ought to enable for a conditional silencing of the goal and an unambiguous identification of engineered most cancers cells. Right here, we current a platform consisting of: (i) t(8;21) and t(15;17) pushed acute myeloid leukemia (AML) transgenic mice with constitutive expression of inexperienced fluorescent protein (GFP) and inducible expression of Cre recombinase, and (ii) REX, a modified pSico lentiviral vector for inducible shRNA expression and crimson fluorescent protein (RFP) as a range marker.
On this system, leukemic cells from transgenic mice are transduced with REX, circulate sorted, and transplanted into syngeneic hosts. Gene interference is induced in established tumors by tamoxifen remedy. Twin-color cell fluorescence guides the in vivo identification of shRNA interfered AML cells, monitoring engraftment and illness development. We examined the platform by inducing knockdown of Zeb2, a gene upregulated by AML1-ETO and PML-RARα oncogenes in pre-leukemic hematopoietic stem cell compartment, and noticed a big delay in leukemia onset. This proves the ability and utility of the platform and confirms Zeb2 contribution to the pathogenesis of AML. Transgenes are susceptible to progressive silencing as a result of their construction, copy quantity, and genomic location. In C. elegans, repressive mechanisms are significantly robust within the germline with nearly totally penetrant transgene silencing in easy extrachromosomal arrays and frequent silencing of single-copy transgene insertions.
A category of non-coding DNA, Periodic An/Tn Clusters (PATCs) can forestall transgene-silencing in repressive chromatin or from small interfering RNAs (piRNAs). Right here, we describe design guidelines (codon-optimization, intron and PATC inclusion, elevated temperature (25 °C), and vector spine elimination) for environment friendly germline expression from arrays in wildtype animals.
BDNF Overexpression Will increase Striatal D3 Receptor Degree at Striatal Neurons and Exacerbates D1-Receptor Agonist-Induced Dyskinesia
Agrobacterium-Mediated Genetic Transformation of Wild Oryza Species Utilizing Immature Embryos
Express Cloning Checker Kit I: Yellow Solution |
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K5011200-2 | Biochain | 1 ml | EUR 75 |
Checker Plus pH Tester - EACH |
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HI98100 | Scientific Laboratory Supplies | EACH | EUR 81 |
SLS Lab Basics pH Checker - EACH |
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PHM9500 | Scientific Laboratory Supplies | EACH | EUR 74.91 |
Hanna Checker pH Tester HI 98103 with 0.1 pH Resolution - EACH |
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PHM4000 | Scientific Laboratory Supplies | EACH | EUR 87.75 |
Mut Express II Fast Mutagenesis Kit V2 |
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C214-01 | Vazyme | 10 rxn | EUR 40.58 |
Mut Express II Fast Mutagenesis Kit V2 |
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C214-02 | Vazyme | 25 rxn | EUR 85.5 |
pCDF1-MCS1 cDNA Cloning and Expression Vector |
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CD100A-1 | SBI | 10 ug | EUR 483 |
pCDH-CMV-MCS cDNA Cloning and Expression Vector |
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CD500B-1 | SBI | 10 ug | EUR 483 |
pCDH-EF1-MCS cDNA Cloning and Expression Vector |
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CD502A-1 | SBI | 10 ug | EUR 483 |
pCDH-CMV-MCS2 cDNA Cloning and Expression Vector |
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CD501A-1 | SBI | 10 ug | EUR 483 |
pSIH1-H1-Puro shRNA Cloning and Expression Vector |
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SI500A-1 | SBI | 10 ug | EUR 507 |
pSIH1-H1-H2Kk shRNA Cloning and Expression Vector |
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SI502A-1 | SBI | 10 ug | EUR 507 |
pAAVK-EF1α -MCS AAVanced Cloning and Expression Vector |
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AAV502A-1 | SBI | 10 ug | EUR 614 |
pSIH1-H1-copGFP shRNA Cloning and Expression Vector |
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SI501A-1 | SBI | 10 ug | EUR 507 |
pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector |
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CD811A-1 | SBI | 10 ug | EUR 579 |
Cumate-Inducible (CuO) EEV cloning and expression vector |
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EEV610A-1 | SBI | 10 ug | EUR 614 |
pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector |
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CD810A-1 | SBI | 10 ug | EUR 579 |
PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector |
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PB511B-1 | SBI | 10 ug | EUR 638 |
PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector |
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PB512B-1 | SBI | 10 ug | EUR 638 |
Constitutive (CAGs promoter) EEV cloning and expression vector |
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EEV600A-1 | SBI | 10 ug | EUR 614 |
PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector |
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PB510B-1 | SBI | 10 ug | EUR 638 |
PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector |
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PB530A-2 | SBI | 10 ug | EUR 638 |
PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
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PB531A-2 | SBI | 10 ug | EUR 638 |
PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector |
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PB533A-2 | SBI | 10 ug | EUR 638 |
pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector |
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CD111B-1 | SBI | 10 ug | EUR 572 |
pCDH-MCS-T2A-copGFP-MSCV Cloning and Expression Vector |
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CD523A-1 | SBI | 10 ug | EUR 590 |
pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector |
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CD512B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector |
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CD514B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
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CD510B-1 | SBI | 10 ug | EUR 572 |
pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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CD530A-2 | SBI | 10 ug | EUR 590 |
pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
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CD531A-2 | SBI | 10 ug | EUR 590 |
pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector |
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CD533A-2 | SBI | 10 ug | EUR 590 |
pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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CD630A-1 | SBI | 10 ug | EUR 590 |
pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector |
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CD711B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
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CD515B-1 | SBI | 10 ug | EUR 572 |
pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector |
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CD522A-1 | SBI | 10 ug | EUR 590 |
pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector |
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CD532A-2 | SBI | 10 ug | EUR 590 |
pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
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CD615B-1 | SBI | 10 ug | EUR 572 |
pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
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CD710B-1 | SBI | 10 ug | EUR 572 |
pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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CD731B-1 | SBI | 10 ug | EUR 590 |
PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector |
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PB514B-2 | SBI | 10 ug | EUR 638 |
pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector |
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CD110B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector |
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CD511B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector |
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CD524A-1 | SBI | 10 ug | EUR 590 |
PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector |
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PB513B-1 | SBI | 10 ug | EUR 638 |
PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector |
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PBSI505A-1 | SBI | 10 ug | EUR 638 |
PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector |
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PBSI506A-1 | SBI | 10 ug | EUR 638 |
pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector |
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CD516B-2 | SBI | 10 ug | EUR 572 |
pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 µg |
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CD527A-1 | SBI | 10 ug | EUR 590 |
pAAVK-EF1α -MCS-T2A-EGFP AAVanced Cloning and Expression Vector |
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AAV526A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α -MCS-T2A-Puro AAVanced Cloning and Expression Vector |
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AAV527A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α –MCS-T2A-mRFP AAVanced Cloning and Expression Vector |
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AAV528A-1 | SBI | 10 ug | EUR 614 |
PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
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PB713B-1 | SBI | 10 ug | EUR 638 |
pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector |
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CD713B-1 | SBI | 10 ug | EUR 572 |
pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector |
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CD813A-1 | SBI | 10 ug | EUR 579 |
pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector |
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CD822A-1 | SBI | 10 ug | EUR 579 |
pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector |
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CD823A-1 | SBI | 10 ug | EUR 579 |
pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
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CD513B-1 | SBI | 10 ug | EUR 572 |
pAAVK-EF1α-MCS1-CMV-MCS2 AAVanced Cloning and Expression Vector |
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AAV503A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α-MCS1-CMV-EGFP AAVanced Cloning and Expression Vector |
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AAV536A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α-MCS1-CMV-Puro AAVanced Cloning and Expression Vector |
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AAV537A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α-MCS1-CMV-mRFP AAVanced Cloning and Expression Vector |
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AAV538A-1 | SBI | 10 ug | EUR 614 |
Jade Express? Mix |
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M1109-500 | Biovision | each | EUR 529.2 |
Express CRISPR sgRNA Synthesis Kit |
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K1253-25 | Biovision | each | EUR 639.6 |
pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector |
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CD550A-1 | SBI | 10 ug | EUR 563 |
XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1-Puro |
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XSTP710PA-1 | SBI | 10 ug | EUR 730 |
PB-CuO-CMV-MCS-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector |
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PBQM800A-1 | SBI | 10 µg | EUR 921 |
Express Plasmid Midiprep Kit (25 min) |
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K1323-10 | Biovision | each | EUR 411.6 |
Express Plasmid Midiprep Kit (25 min) |
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K1323-2 | Biovision | each | EUR 314.4 |
Express Plasmid Midiprep Kit (25 min) |
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K1323-25 | Biovision | each | EUR 561.6 |
Express Plasmid Maxiprep Kit (25 min) |
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K1324-10 | Biovision | each | EUR 510 |
Express Plasmid Maxiprep Kit (25 min) |
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K1324-2 | Biovision | each | EUR 339.6 |
Express Plasmid Maxiprep Kit (25 min) |
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K1324-25 | Biovision | each | EUR 732 |
PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector |
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PBQM812A-1 | SBI | 10 ug | EUR 1023 |
ToxOut? Express Endofree Plasmid Midi Kit |
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K1335-10 | Biovision | each | EUR 444 |
ToxOut? Express Endofree Plasmid Midi Kit |
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K1335-2 | Biovision | each | EUR 280.8 |
ToxOut? Express Endofree Plasmid Midi Kit |
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K1335-25 | Biovision | each | EUR 836.4 |
ToxOut? Express Endofree Plasmid Maxi Kit |
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K1336-10 | Biovision | each | EUR 574.8 |
ToxOut? Express Endofree Plasmid Maxi Kit |
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K1336-2 | Biovision | each | EUR 314.4 |
ToxOut? Express Endofree Plasmid Maxi Kit |
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K1336-25 | Biovision | each | EUR 1032 |
Jade Express? Mix-ROX |
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M1112-500 | Biovision | each | EUR 529.2 |
Staph Express Disk - PK20 |
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FSA1046 | Scientific Laboratory Supplies | PK20 | EUR 56.7 |
Staph Express Disk - PK100 |
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FSA1044 | Scientific Laboratory Supplies | PK100 | EUR 151.29 |
Cryo Express Dry Shipper |
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CC-CX100 | Jena Bioscience GmbH | 1pc. | EUR 1787.1 |
Cryo Express Dry Shipper |
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CC-CXR100 | Jena Bioscience GmbH | 1pc. | EUR 1905.3 |
pDsRed- Express- C1 Plasmid |
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PVT1236 | Lifescience Market | 2 ug | EUR 319.2 |
PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector |
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PBQMSH812A-1 | SBI | 10 ug | EUR 1023 |
Jade Express? Mix-iCycler |
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M1110-500 | Biovision | each | EUR 529.2 |
pIRES2- DsRed- Express Plasmid |
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PVT1229 | Lifescience Market | 2 ug | EUR 289.2 |
Opti-Protein Express Marker |
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E36G494 | EnoGene | 500 μl/100 loads | EUR 57.14 |
Mut Express MultiS Fast Mutagenesis Kit V2 |
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C215-01 | Vazyme | 10 rxn | EUR 92.66 |
Mut Express MultiS Fast Mutagenesis Kit V2 |
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C215-02 | Vazyme | 25 rxn | EUR 194 |
Jade Express? Mix-Low ROX |
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M1111-500 | Biovision | each | EUR 529.2 |
Star 1kb DNA Ladder Express |
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BT10701 | Bioatlas | 500µl | EUR 96 |
Description: High purity buffer for various PCR applications. |
Staph Express Count Plate - PK50 |
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FSA1042 | Scientific Laboratory Supplies | PK50 | EUR 132.3 |
Cryo Express Dry Shipper (CX100) |
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TW-CX100 | MiTeGen | 1 SHIPPER | EUR 1265 |
Description: Cryo Express Dry Shipper (CX100) |
Staph Express Count Plate - PK500 |
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FSA1040 | Scientific Laboratory Supplies | PK500 | EUR 931.5 |
Express Coomassie Stain G-250 |
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40340020-1 | Glycomatrix | 1 L | EUR 99.76 |
Express Coomassie Stain G-250 |
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40340020-2 | Glycomatrix | 500 mL | EUR 68.06 |
Express Coomassie Stain, R-250 |
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40340024-1 | Glycomatrix | 100 mL | EUR 52.41 |
MILLIPAK EXPRESS 40 FILTER - EACH |
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MPGP04001SIG | Scientific Laboratory Supplies | EACH | EUR 293.76 |
Star 100 bp DNA Ladder Express |
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BA01302 | Bioatlas | 500µl | EUR 96 |
Description: High purity buffer for various PCR applications. |
ClonExpress II One Step Cloning Kit |
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C112-01 | Vazyme | 25 rxn | EUR 108.6 |
ClonExpress II One Step Cloning Kit |
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C112-02 | Vazyme | 50 rxn | EUR 181 |
BrightGreen Express 2X qPCR MasterMix-ROX |
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MasterMix-ER | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
Millipak Express 20 Filter (1/box) - EACH |
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MPGP02001 | Scientific Laboratory Supplies | EACH | EUR 314.32 |
BrightGreen Express 2X qPCR MasterMix-iCycler |
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MasterMix-EC | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
Cryo Express Dry Shipper & Alu Shipping Case |
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CC-CX100ASC | Jena Bioscience GmbH | 1 + 1pc. | EUR 2434.6 |
BrightGreen Express 2X qPCR MasterMix-No Dye |
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MasterMix-ES | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
BrightGreen Express 2X qPCR MasterMix-Low ROX |
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MasterMix-EL | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
T1 Cloning Kit |
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20-abx098055 | Abbexa |
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T3 Cloning Kit |
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20-abx098057 | Abbexa |
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T1 Cloning Kit |
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abx098055-100l | Abbexa | 100 µl | EUR 737.5 |
T1 Cloning Kit |
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abx098055-1ml | Abbexa | 1 ml | Ask for price |
T1 Cloning Kit |
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abx098055-200l | Abbexa | 200 µl | EUR 950 |
T3 Cloning Kit |
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abx098057-100l | Abbexa | 100 µl | EUR 737.5 |
T3 Cloning Kit |
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abx098057-1ml | Abbexa | 1 ml | Ask for price |
T3 Cloning Kit |
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abx098057-200l | Abbexa | 200 µl | EUR 950 |
Blunt Cloning Kit |
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20-abx098059 | Abbexa |
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Blunt Cloning Kit |
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abx098059-100l | Abbexa | 100 µl | EUR 737.5 |
Blunt Cloning Kit |
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abx098059-1ml | Abbexa | 1 ml | Ask for price |
Blunt Cloning Kit |
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abx098059-200l | Abbexa | 200 µl | EUR 950 |