A Novel Platform to Test In Vivo Single Gene Dependencies in t(8,21) and t(15,17) AML Confirms Zeb2 as Leukemia Target

A Novel Platform to Test In Vivo Single Gene Dependencies in t(8,21) and t(15,17) AML Confirms Zeb2 as Leukemia Target

The elevated utilization of high-throughput applied sciences in most cancers analysis, together with genetic and drug screens, generates massive units of candidate targets that should be functionally validated for his or her roles in tumor growth. Thus, dependable and strong in vivo mannequin methods are wanted to carry out reverse genetic experiments. Ideally, these fashions ought to enable for a conditional silencing of the goal and an unambiguous identification of engineered most cancers cells. Right here, we current a platform consisting of: (i) t(8;21) and t(15;17) pushed acute myeloid leukemia (AML) transgenic mice with constitutive expression of inexperienced fluorescent protein (GFP) and inducible expression of Cre recombinase, and (ii) REX, a modified pSico lentiviral vector for inducible shRNA expression and crimson fluorescent protein (RFP) as a range marker.

On this system, leukemic cells from transgenic mice are transduced with REX, circulate sorted, and transplanted into syngeneic hosts. Gene interference is induced in established tumors by tamoxifen remedy. Twin-color cell fluorescence guides the in vivo identification of shRNA interfered AML cells, monitoring engraftment and illness development. We examined the platform by inducing knockdown of Zeb2, a gene upregulated by AML1-ETO and PML-RARα oncogenes in pre-leukemic hematopoietic stem cell compartment, and noticed a big delay in leukemia onset. This proves the ability and utility of the platform and confirms Zeb2 contribution to the pathogenesis of AML. Transgenes are susceptible to progressive silencing as a result of their construction, copy quantity, and genomic location. In C. elegans, repressive mechanisms are significantly robust within the germline with nearly totally penetrant transgene silencing in easy extrachromosomal arrays and frequent silencing of single-copy transgene insertions.

A category of non-coding DNA, Periodic An/Tn Clusters (PATCs) can forestall transgene-silencing in repressive chromatin or from small interfering RNAs (piRNAs). Right here, we describe design guidelines (codon-optimization, intron and PATC inclusion, elevated temperature (25 °C), and vector spine elimination) for environment friendly germline expression from arrays in wildtype animals.

 

 

BDNF Overexpression Will increase Striatal D3 Receptor Degree at Striatal Neurons and Exacerbates D1-Receptor Agonist-Induced Dyskinesia

Background: We just lately confirmed that striatal overexpression of mind derived neurotrophic issue (BDNF) by adeno-associated viral (AAV) vector exacerbated L-DOPA-induced dyskinesia (LID) in 6-OHDA-lesioned rats. An intensive sprouting of striatal serotonergic terminals accompanied this impact, accounting for the elevated susceptibility to LID.
Goal: We set to research whether or not the BDNF impact was restricted to LID, or prolonged to dyskinesia induced by direct D1 receptor agonists.
Strategies: Unilaterally 6-OHDA-lesioned rats obtained a striatal injection of an AAV vector to induce BDNF overexpression. Eight weeks later, animals obtained every day remedies with a low dose of SKF82958 (0.02 mg/kg s.c.) and growth of dyskinesia was evaluated. On the finish of the experiment, D1 and D3 receptors expression ranges and D1 receptor-dependent signaling pathways have been measured within the striatum.
Outcomes: BDNF overexpression induced important worsening of dyskinesia induced by SKF82958 in comparison with the GFP group and elevated the expression of D3 receptor at striatal degree, even in absence of pharmacological remedy; in contrast, D1 receptor ranges weren’t affected. In BDNF-overexpressing striata, SKF82958 administration resulted in elevated ranges of D1-D3 receptors co-immunoprecipitation and elevated phosphorylation ranges of Thr34 DARPP-32 and ERK1/2.
Conclusion: Right here we offer proof for a purposeful hyperlink between BDNF, D3 receptors and D1-D3 receptor shut interplay within the augmented susceptibility to dyskinesia in 6-OHDA-lesioned rats. We propose that D1/D3 receptors interplay could also be instrumental in driving the molecular alterations underlying the looks of dyskinesia; its disruption could also be a therapeutic technique for treating dyskinesia in PD sufferers.
 A Novel Platform to Test In Vivo Single Gene Dependencies in t(8,21) and t(15,17) AML Confirms Zeb2 as Leukemia Target

A Novel Platform to Test In Vivo Single Gene Dependencies in t(8,21) and t(15,17) AML Confirms Zeb2 as Leukemia Target

Agrobacterium-Mediated Genetic Transformation of Wild Oryza Species Utilizing Immature Embryos

Genetic transformation is likely one of the most essential applied sciences for revealing or modulating gene perform. It’s used extensively in each purposeful genomics and molecular breeding of rice. Calls for on its use in wild Oryza species is rising due to their excessive genetic range. Given the difficulties in genetic crosses between distantly associated species, genetic transformation provides a option to alter or switch genetic traits in wild rice accessions. Nevertheless, transformation of untamed Oryza accessions by typical strategies utilizing calli induced from scutellum tissue of embryos in mature seeds usually fails.
Right here, we report strategies utilizing immature embryos for the genetic transformation of a broad vary of Oryza species. First, we investigated the power of callus induction and regeneration from immature embryos of 192 accessions in 20 species below a number of tradition situations. We regenerated vegetation from immature embryos of 90 accessions in 16 species. Subsequent, we optimized the situations of Agrobacterium an infection utilizing a vector carrying the GFP gene pushed by the maize ubiquitin promoter. GFP alerts have been noticed in 51 accessions in 11 species. We analyzed the expansion and seed set of transgenic vegetation of O. barthii, O. glumaepatula, O. rufipogon, and O. brachyantha. The vegetation grew to maturity and set seeds usually. Southern blot analyses utilizing DNA from T0 vegetation confirmed that every one GFP vegetation have been derived from impartial transformation occasions.

Express Cloning Checker Kit I: Yellow Solution

K5011200-2 1 ml
EUR 130.8

Mut Express II Fast Mutagenesis Kit V2

C214-01 10 rxn
EUR 198

Mut Express II Fast Mutagenesis Kit V2

C214-02 25 rxn
EUR 283.2

Human Drug Detoxication II

HDTX-II 1 set
EUR 774

Human Kinase Library II

HKIN-II 1 set
EUR 540

Mouse Cytokine Primer Library II

MCA-II 1 set
EUR 540

Human Cytokine Primer Library II

HCA-II 1 set
EUR 540

Rat Cytokine Primer Library II

RCA-II 1 set
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Mouse DNA Repair Primer Library II

MDRL-II 1 set
EUR 774

Human DNA Repair Primer Library II

HDRL-II 1 set
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Human Colon Cancer Primer Library II

HCCP-II 1 set
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Human Cancer Driver Gene II Primer Library

HCDG-II 1 set
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Human Interferon Type II Signaling Primer Library

HIFN-II 1 set
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PrecisionX Multiplex gRNA Cloning Kit

CAS9-GRNA-KIT 10 rxn
EUR 534

Checker Plus pH Tester

HI98100 EACH
EUR 55.75

Jade Express? Mix

M1109-500
EUR 529.2

Staph Express Disk

FSA1044 PK100
EUR 117.42

Staph Express Disk

FSA1046 PK20
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Express CRISPR sgRNA Synthesis Kit

K1253-25
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SLS Lab Basics pH Checker

PHM9500 EACH
EUR 55.29

ClonExpress II One Step Cloning Kit

C112-01 25 rxn
EUR 202.8

ClonExpress II One Step Cloning Kit

C112-02 50 rxn
EUR 259.2

ToxOut? Express Endofree Plasmid Midi Kit

K1335-10
EUR 444

ToxOut? Express Endofree Plasmid Midi Kit

K1335-2
EUR 280.8

ToxOut? Express Endofree Plasmid Midi Kit

K1335-25
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ToxOut? Express Endofree Plasmid Maxi Kit

K1336-10
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ToxOut? Express Endofree Plasmid Maxi Kit

K1336-2
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ToxOut? Express Endofree Plasmid Maxi Kit

K1336-25
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Express Plasmid Midiprep Kit (25 min)

K1323-10
EUR 411.6

Express Plasmid Midiprep Kit (25 min)

K1323-2
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Express Plasmid Midiprep Kit (25 min)

K1323-25
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Express Plasmid Maxiprep Kit (25 min)

K1324-10
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Express Plasmid Maxiprep Kit (25 min)

K1324-2
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Express Plasmid Maxiprep Kit (25 min)

K1324-25
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Jade Express? Mix-iCycler

M1110-500
EUR 529.2

Jade Express? Mix-ROX

M1112-500
EUR 529.2

pIRES2- DsRed- Express Plasmid

PVT1229 2 ug
EUR 289.2

pDsRed- Express- C1 Plasmid

PVT1236 2 ug
EUR 319.2

MILLIPAK EXPRESS 40 FILTER

MPGP04001SIG EACH
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Staph Express Count Plate

FSA1040 PK500
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Staph Express Count Plate

FSA1042 PK50
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Mut Express MultiS Fast Mutagenesis Kit V2

C215-01 10 rxn
EUR 297.6

Mut Express MultiS Fast Mutagenesis Kit V2

C215-02 25 rxn
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Star 1kb DNA Ladder Express

BT10701 500µl
EUR 96
Description: High purity buffer for various PCR applications.

Jade Express? Mix-Low ROX

M1111-500
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Cryo Express Dry Shipper (CX100)

TW-CX100 1 SHIPPER
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Description: Cryo Express Dry Shipper (CX100)

Frit Kit

FRIT-KIT 1each
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Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

RapidSeq High Yield Directional mRNA Sample Prep Kit - With Aligner 13-24

KS073012-II 1 Kit
EUR 1370.4
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

RapidSeq High Yield Small RNA Sample Prep Kit - With Aligner 13-24

KS074012-II 1 Kit
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Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti rabbit secondary antibody

K3171120-II 1200 cm2
EUR 295.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti rabbit secondary antibody

K3171250-II 2500 cm2
EUR 372
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Column Packing Kit

PACK-KIT 1pack
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Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit

M034-Kit Kit
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Star 100 bp DNA Ladder Express

BA01302 500µl
EUR 96
Description: High purity buffer for various PCR applications.

BrightGreen Express 2X qPCR MasterMix-iCycler

MasterMix-EC 4 x 1.25 ml for 500 reactions (20 ul)
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BrightGreen Express 2X qPCR MasterMix-ROX

MasterMix-ER 4 x 1.25 ml for 500 reactions (20 ul)
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Millipak Express 20 Filter (1/box)

MPGP02001 EACH
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Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
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Pesticide Kit Containing All 10 Multi-Compound Mixes

SPXPR-KIT 1ML
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CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
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CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
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MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
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MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
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Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
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Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
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T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
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Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
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Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT 1 Kit
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Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
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Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
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Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
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Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
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Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
EUR 1358.4

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 183.6

Claritas PPT Grade Minerals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXMT-KIT 30ML
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PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
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Claritas PPT Grade Heavy Metals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXHM-KIT 30ML
EUR 245.1

Aligner 13-24

KS072012-II 1 Kit
EUR 494.4
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

BrightGreen Express 2X qPCR MasterMix-Low ROX

MasterMix-EL 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

BrightGreen Express 2X qPCR MasterMix-No Dye

MasterMix-ES 4 x 1.25 ml for 500 reactions (20 ul)
EUR 168

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 5929.2

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
EUR 2558.4

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT 1 kit
EUR 2558.4

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
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Hanna Checker pH Tester HI 98103 with 0.1 pH Resolution

PHM4000 EACH
EUR 61.56

vWF Acty. Kit

ABP-ACT-KIT 12 x 8 microwells
EUR 513.6

vWF Ant. Kit

ABP-TOT-KIT 12 x 8 microwells
EUR 472.8

hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9)

CAS620A-KIT 1 kit
EUR 2582.4
Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression)

PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN300A-KIT 1 Kit
EUR 3357.6

PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN400A-KIT 1 Kit
EUR 3357.6

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN410A-KIT 1 Kit
EUR 5202

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)

PIN412A-KIT 1 Kit
EUR 5202

Filtration System 47mm Complete Assembly

5809/KIT EACH
EUR 427.69

Angiotensin II (Ang II) ELISA Kit

abx511338-96tests 96 tests
EUR 801.6

mRNAExpress mRNA Synthesis kit (5 reactions)

MR-KIT-1 5 reactions
EUR 1382.4

42 Component Terpene Kit in Methanol

CAN-TERP-KIT 1ML
EUR 287.28

Canadian Pesticide Kit Containing All 6 Mixes

CAN-CAN-KIT 1ML
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AxioCam Erc5s rev 2, w/Accessory Kit

ZV-Erc5sAC-Kit 1 KIT
EUR 1611
Description: AxioCam Erc5s rev 2, w/Accessory Kit- connection cable Y-USB 2.0.- HDMI cable to DVI-D- SD card- IR remote control

Cryo Express Dry Shipper with replaceable absorbent Material (CXR100)

TW-CXR100 1 SHIPPER
EUR 1380
Description: Cryo Express Dry Shipper with replaceable absorbent Material (CXR100)

T1 Cloning Kit

20-abx098055
  • EUR 594.00
  • EUR 794.40
  • 20 rxns
  • 60 rxns

T3 Cloning Kit

20-abx098057
  • EUR 594.00
  • EUR 794.40
  • 20 rxns
  • 60 rxns

Blunt Cloning Kit

20-abx098059
  • EUR 594.00
  • EUR 794.40
  • 20 rxns
  • 60 rxns

Blunt3 Cloning Kit

20-abx098061
  • EUR 594.00
  • EUR 794.40
  • 20 rxns
  • 60 rxns

General Ang-II/ Angiotensin II ELISA Kit

E0086Ge 1 Kit
EUR 675.6

Human ANG II(Angiotensin II) ELISA Kit

EH2627 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

Human MHC II/RLA II ELISA Kit

EHM0285 96Tests
EUR 625.2

Porcine MHC II/RLA II ELISA Kit

EPM0285 96Tests
EUR 625.2

Rat ANG II (Angiotensin II) ELISA Kit

ER1637 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml

Rabbit ANG II(Angiotensin II) ELISA Kit

ERB0006 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rabbit;Sensitivity: 18.75pg/ml

Mouse MHC II/RLA II ELISA Kit

EMM0285 96Tests
EUR 625.2

Rat MHC II/RLA II ELISA Kit

ERM0285 96Tests
EUR 625.2

Rabbit MHC II/RLA II ELISA Kit

ERTM0285 96Tests
EUR 625.2

Rabbit Angiotensin II (Ang II) ELISA Kit

20-abx155034
  • EUR 8684.40
  • EUR 4626.00
  • EUR 1074.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Angiotensin II (Ang II) ELISA Kit

20-abx155198
  • EUR 8684.40
  • EUR 4626.00
  • EUR 1074.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Chicken Angiotensin II (Ang II) ELISA Kit

20-abx150002
  • EUR 8684.40
  • EUR 4626.00
  • EUR 1074.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Cow Angiotensin II (Ang II) ELISA Kit

20-abx150070
  • EUR 9174.00
  • EUR 4888.80
  • EUR 1130.40
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Horse Angiotensin II (Ang II) ELISA Kit

20-abx150439
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Angiotensin II (Ang II) ELISA Kit

20-abx150654
  • EUR 7970.40
  • EUR 4250.40
  • EUR 990.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Angiotensin II (Ang II) ELISA Kit

20-abx153637
  • EUR 7970.40
  • EUR 4250.40
  • EUR 990.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Angiotensin II (Ang II) ELISA Kit

20-abx052349
  • EUR 8684.40
  • EUR 4626.00
  • EUR 1074.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Angiotensin II (ANG II) CLIA Kit

abx195117-96tests 96 tests
EUR 990
We confirmed that the T-DNAs have been transmitted to the following technology via the segregation of GFP alerts within the T1 technology. These outcomes present that many Oryza species could be remodeled by utilizing modified immature-embryo strategies. It will speed up the usage of wild Oryza accessions in molecular genetic analyses and molecular breeding.

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