The elevated utilization of high-throughput applied sciences in most cancers analysis, together with genetic and drug screens, generates massive units of candidate targets that should be functionally validated for his or her roles in tumor growth. Thus, dependable and strong in vivo mannequin methods are wanted to carry out reverse genetic experiments. Ideally, these fashions ought to enable for a conditional silencing of the goal and an unambiguous identification of engineered most cancers cells. Right here, we current a platform consisting of: (i) t(8;21) and t(15;17) pushed acute myeloid leukemia (AML) transgenic mice with constitutive expression of inexperienced fluorescent protein (GFP) and inducible expression of Cre recombinase, and (ii) REX, a modified pSico lentiviral vector for inducible shRNA expression and crimson fluorescent protein (RFP) as a range marker.
On this system, leukemic cells from transgenic mice are transduced with REX, circulate sorted, and transplanted into syngeneic hosts. Gene interference is induced in established tumors by tamoxifen remedy. Twin-color cell fluorescence guides the in vivo identification of shRNA interfered AML cells, monitoring engraftment and illness development. We examined the platform by inducing knockdown of Zeb2, a gene upregulated by AML1-ETO and PML-RARα oncogenes in pre-leukemic hematopoietic stem cell compartment, and noticed a big delay in leukemia onset. This proves the ability and utility of the platform and confirms Zeb2 contribution to the pathogenesis of AML. Transgenes are susceptible to progressive silencing as a result of their construction, copy quantity, and genomic location. In C. elegans, repressive mechanisms are significantly robust within the germline with nearly totally penetrant transgene silencing in easy extrachromosomal arrays and frequent silencing of single-copy transgene insertions.
A category of non-coding DNA, Periodic An/Tn Clusters (PATCs) can forestall transgene-silencing in repressive chromatin or from small interfering RNAs (piRNAs). Right here, we describe design guidelines (codon-optimization, intron and PATC inclusion, elevated temperature (25 °C), and vector spine elimination) for environment friendly germline expression from arrays in wildtype animals.
BDNF Overexpression Will increase Striatal D3 Receptor Degree at Striatal Neurons and Exacerbates D1-Receptor Agonist-Induced Dyskinesia
Background: We just lately confirmed that striatal overexpression of mind derived neurotrophic issue (BDNF) by adeno-associated viral (AAV) vector exacerbated L-DOPA-induced dyskinesia (LID) in 6-OHDA-lesioned rats. An intensive sprouting of striatal serotonergic terminals accompanied this impact, accounting for the elevated susceptibility to LID.
Goal: We set to research whether or not the BDNF impact was restricted to LID, or prolonged to dyskinesia induced by direct D1 receptor agonists.
Strategies: Unilaterally 6-OHDA-lesioned rats obtained a striatal injection of an AAV vector to induce BDNF overexpression. Eight weeks later, animals obtained every day remedies with a low dose of SKF82958 (0.02 mg/kg s.c.) and growth of dyskinesia was evaluated. On the finish of the experiment, D1 and D3 receptors expression ranges and D1 receptor-dependent signaling pathways have been measured within the striatum.
Outcomes: BDNF overexpression induced important worsening of dyskinesia induced by SKF82958 in comparison with the GFP group and elevated the expression of D3 receptor at striatal degree, even in absence of pharmacological remedy; in contrast, D1 receptor ranges weren’t affected. In BDNF-overexpressing striata, SKF82958 administration resulted in elevated ranges of D1-D3 receptors co-immunoprecipitation and elevated phosphorylation ranges of Thr34 DARPP-32 and ERK1/2.
Conclusion: Right here we offer proof for a purposeful hyperlink between BDNF, D3 receptors and D1-D3 receptor shut interplay within the augmented susceptibility to dyskinesia in 6-OHDA-lesioned rats. We propose that D1/D3 receptors interplay could also be instrumental in driving the molecular alterations underlying the looks of dyskinesia; its disruption could also be a therapeutic technique for treating dyskinesia in PD sufferers.
A Novel Platform to Test In Vivo Single Gene Dependencies in t(8,21) and t(15,17) AML Confirms Zeb2 as Leukemia Target
Agrobacterium-Mediated Genetic Transformation of Wild Oryza Species Utilizing Immature Embryos
Genetic transformation is likely one of the most essential applied sciences for revealing or modulating gene perform. It’s used extensively in each purposeful genomics and molecular breeding of rice. Calls for on its use in wild Oryza species is rising due to their excessive genetic range. Given the difficulties in genetic crosses between distantly associated species, genetic transformation provides a option to alter or switch genetic traits in wild rice accessions. Nevertheless, transformation of untamed Oryza accessions by typical strategies utilizing calli induced from scutellum tissue of embryos in mature seeds usually fails.
Right here, we report strategies utilizing immature embryos for the genetic transformation of a broad vary of Oryza species. First, we investigated the power of callus induction and regeneration from immature embryos of 192 accessions in 20 species below a number of tradition situations. We regenerated vegetation from immature embryos of 90 accessions in 16 species. Subsequent, we optimized the situations of Agrobacterium an infection utilizing a vector carrying the GFP gene pushed by the maize ubiquitin promoter. GFP alerts have been noticed in 51 accessions in 11 species. We analyzed the expansion and seed set of transgenic vegetation of O. barthii, O. glumaepatula, O. rufipogon, and O. brachyantha. The vegetation grew to maturity and set seeds usually. Southern blot analyses utilizing DNA from T0 vegetation confirmed that every one GFP vegetation have been derived from impartial transformation occasions.
Express Cloning Checker Kit I: Yellow Solution |
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| K5011200-2 | Biochain | 1 ml | EUR 54.95 |
Checker Plus pH Tester - EACH |
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| HI98100 | Scientific Laboratory Supplies | EACH | EUR 81 |
SLS Lab Basics pH Checker - EACH |
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| PHM9500 | Scientific Laboratory Supplies | EACH | EUR 74.91 |
pCDF1-MCS1 cDNA Cloning and Expression Vector |
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| CD100A-1 | SBI | 10 ug | EUR 483 |
pCDH-CMV-MCS cDNA Cloning and Expression Vector |
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| CD500B-1 | SBI | 10 ug | EUR 483 |
pCDH-EF1-MCS cDNA Cloning and Expression Vector |
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| CD502A-1 | SBI | 10 ug | EUR 483 |
pCDH-CMV-MCS2 cDNA Cloning and Expression Vector |
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| CD501A-1 | SBI | 10 ug | EUR 483 |
pSIH1-H1-Puro shRNA Cloning and Expression Vector |
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| SI500A-1 | SBI | 10 ug | EUR 507 |
pSIH1-H1-H2Kk shRNA Cloning and Expression Vector |
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| SI502A-1 | SBI | 10 ug | EUR 507 |
Hanna Checker pH Tester HI 98103 with 0.1 pH Resolution - EACH |
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| PHM4000 | Scientific Laboratory Supplies | EACH | EUR 87.75 |
pAAVK-EF1α -MCS AAVanced Cloning and Expression Vector |
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| AAV502A-1 | SBI | 10 ug | EUR 614 |
pSIH1-H1-copGFP shRNA Cloning and Expression Vector |
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| SI501A-1 | SBI | 10 ug | EUR 507 |
pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector |
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| CD811A-1 | SBI | 10 ug | EUR 579 |
Cumate-Inducible (CuO) EEV cloning and expression vector |
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| EEV610A-1 | SBI | 10 ug | EUR 614 |
pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector |
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| CD810A-1 | SBI | 10 ug | EUR 579 |
Constitutive (CAGs promoter) EEV cloning and expression vector |
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| EEV600A-1 | SBI | 10 ug | EUR 614 |
PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector |
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| PB511B-1 | SBI | 10 ug | EUR 638 |
PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector |
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| PB512B-1 | SBI | 10 ug | EUR 638 |
PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector |
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| PB510B-1 | SBI | 10 ug | EUR 638 |
PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector |
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| PB530A-2 | SBI | 10 ug | EUR 638 |
PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
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| PB531A-2 | SBI | 10 ug | EUR 638 |
PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector |
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| PB533A-2 | SBI | 10 ug | EUR 638 |
pCDH-MCS-T2A-copGFP-MSCV Cloning and Expression Vector |
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| CD523A-1 | SBI | 10 ug | EUR 590 |
pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector |
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| CD111B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector |
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| CD512B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector |
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| CD514B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
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| CD510B-1 | SBI | 10 ug | EUR 572 |
pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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| CD530A-2 | SBI | 10 ug | EUR 590 |
pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
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| CD531A-2 | SBI | 10 ug | EUR 590 |
pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector |
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| CD533A-2 | SBI | 10 ug | EUR 590 |
pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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| CD630A-1 | SBI | 10 ug | EUR 590 |
pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector |
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| CD711B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
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| CD515B-1 | SBI | 10 ug | EUR 572 |
pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector |
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| CD522A-1 | SBI | 10 ug | EUR 590 |
pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector |
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| CD532A-2 | SBI | 10 ug | EUR 590 |
pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
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| CD615B-1 | SBI | 10 ug | EUR 572 |
pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
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| CD710B-1 | SBI | 10 ug | EUR 572 |
pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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| CD731B-1 | SBI | 10 ug | EUR 590 |
PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector |
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| PB514B-2 | SBI | 10 ug | EUR 638 |
pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector |
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| CD110B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector |
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| CD511B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector |
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| CD524A-1 | SBI | 10 ug | EUR 590 |
PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector |
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| PB513B-1 | SBI | 10 ug | EUR 638 |
PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector |
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| PBSI505A-1 | SBI | 10 ug | EUR 638 |
PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector |
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| PBSI506A-1 | SBI | 10 ug | EUR 638 |
pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector |
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| CD516B-2 | SBI | 10 ug | EUR 572 |
pAAVK-EF1α -MCS-T2A-EGFP AAVanced Cloning and Expression Vector |
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| AAV526A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α -MCS-T2A-Puro AAVanced Cloning and Expression Vector |
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| AAV527A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α –MCS-T2A-mRFP AAVanced Cloning and Expression Vector |
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| AAV528A-1 | SBI | 10 ug | EUR 614 |
PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
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| PB713B-1 | SBI | 10 ug | EUR 638 |
pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector |
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| CD713B-1 | SBI | 10 ug | EUR 572 |
pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector |
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| CD813A-1 | SBI | 10 ug | EUR 579 |
pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector |
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| CD822A-1 | SBI | 10 ug | EUR 579 |
pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector |
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| CD823A-1 | SBI | 10 ug | EUR 579 |
pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 µg |
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| CD527A-1 | SBI | 10 ug | EUR 590 |
pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
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| CD513B-1 | SBI | 10 ug | EUR 572 |
pAAVK-EF1α-MCS1-CMV-MCS2 AAVanced Cloning and Expression Vector |
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| AAV503A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α-MCS1-CMV-EGFP AAVanced Cloning and Expression Vector |
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| AAV536A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α-MCS1-CMV-Puro AAVanced Cloning and Expression Vector |
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| AAV537A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α-MCS1-CMV-mRFP AAVanced Cloning and Expression Vector |
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| AAV538A-1 | SBI | 10 ug | EUR 614 |
Mut Express II Fast Mutagenesis Kit V2 |
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| C214-01 | Vazyme | 10 rxn | EUR 40.58 |
Mut Express II Fast Mutagenesis Kit V2 |
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| C214-01-10rxns | Vazyme | 10 rxns | EUR 42.02 |
Mut Express II Fast Mutagenesis Kit V2 |
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| C214-02 | Vazyme | 25 rxn | EUR 85.5 |
Mut Express II Fast Mutagenesis Kit V2 |
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| C214-02-25rxns | Vazyme | 25 rxns | EUR 88.54 |
XStamp cloning and expression lentivector MSCV-Leader-MCS-C1C2-EF1-Puro |
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| XSTP710PA-1 | SBI | 10 ug | EUR 730 |
pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector |
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| CD550A-1 | SBI | 10 ug | EUR 563 |
Novel Express qPCR System - ROX II |
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| MBS402063-100Reactions | MyBiosource | 100Reactions | EUR 220 |
Novel Express qPCR System - ROX II |
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| MBS402063-20Reactions | MyBiosource | 20Reactions | EUR 170 |
PB-CuO-CMV-MCS-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector |
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| PBQM800A-1 | SBI | 10 µg | EUR 921 |
PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector |
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| PBQM812A-1 | SBI | 10 ug | EUR 1023 |
PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector |
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| PBQMSH812A-1 | SBI | 10 ug | EUR 1023 |
Jade Express? Mix |
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| M1109-500 | Biovision | each | EUR 529.2 |
Express Endofree Plasmid Maxi Kit |
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| EP-011 | Creative BioMart | 2 preparations | EUR 439.2 |
Express Endofree Plasmid Midi Kit |
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| EP-012 | Creative BioMart | 10 preparations | EUR 519.2 |
Express CRISPR sgRNA Synthesis Kit |
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| K1253-25 | Biovision | each | EUR 639.6 |
Express Plasmid Midiprep Kit (25 min) |
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| K1323-10 | Biovision | each | EUR 411.6 |
Express Plasmid Midiprep Kit (25 min) |
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| K1323-2 | Biovision | each | EUR 314.4 |
Express Plasmid Midiprep Kit (25 min) |
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| K1323-25 | Biovision | each | EUR 561.6 |
Express Plasmid Maxiprep Kit (25 min) |
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| K1324-10 | Biovision | each | EUR 510 |
Express Plasmid Maxiprep Kit (25 min) |
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| K1324-2 | Biovision | each | EUR 339.6 |
Express Plasmid Maxiprep Kit (25 min) |
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| K1324-25 | Biovision | each | EUR 732 |
Jade Express? Mix-ROX |
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| M1112-500 | Biovision | each | EUR 529.2 |
ToxOut? Express Endofree Plasmid Midi Kit |
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| K1335-10 | Biovision | each | EUR 444 |
ToxOut? Express Endofree Plasmid Midi Kit |
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| K1335-2 | Biovision | each | EUR 280.8 |
ToxOut? Express Endofree Plasmid Midi Kit |
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| K1335-25 | Biovision | each | EUR 836.4 |
ToxOut? Express Endofree Plasmid Maxi Kit |
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| K1336-10 | Biovision | each | EUR 574.8 |
ToxOut? Express Endofree Plasmid Maxi Kit |
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| K1336-2 | Biovision | each | EUR 314.4 |
ToxOut? Express Endofree Plasmid Maxi Kit |
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| K1336-25 | Biovision | each | EUR 1032 |
Staph Express Disk - PK20 |
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| FSA1046 | Scientific Laboratory Supplies | PK20 | EUR 56.7 |
Cryo Express Dry Shipper |
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| CC-CX100 | Jena Bioscience GmbH | 1pc. | EUR 1737.06 |
Cryo Express Dry Shipper |
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| CC-CXR100 | Jena Bioscience GmbH | 1pc. | EUR 1851.95 |
Staph Express Disk - PK100 |
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| FSA1044 | Scientific Laboratory Supplies | PK100 | EUR 151.29 |
pDsRed- Express- C1 Plasmid |
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| PVT1236 | Lifescience Market | 2 ug | EUR 319.2 |
Jade Express? Mix-iCycler |
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| M1110-500 | Biovision | each | EUR 529.2 |
ClonExpress II One Step Cloning Kit |
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| C112-00-5rxns | Vazyme | 5 rxns | EUR 1.09 |
ClonExpress II One Step Cloning Kit |
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| C112-01 | Vazyme | 25 rxn | EUR 108.6 |
ClonExpress II One Step Cloning Kit |
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| C112-01-25rxns | Vazyme | 25 rxns | EUR 112.46 |
ClonExpress II One Step Cloning Kit |
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| C112-02 | Vazyme | 50 rxn | EUR 181 |
ClonExpress II One Step Cloning Kit |
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| C112-02-50rxns | Vazyme | 50 rxns | EUR 187.43 |
pIRES2- DsRed- Express Plasmid |
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| PVT1229 | Lifescience Market | 2 ug | EUR 289.2 |
Opti-Protein Express Marker |
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| E36G494 | EnoGene | 500 μl/100 loads | EUR 57.14 |
Opti-Protein Express Marker |
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| MBS8580110-05mL | MyBiosource | 0.5mL | EUR 155 |
Opti-Protein Express Marker |
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| MBS8580110-5x05mL | MyBiosource | 5x0.5mL | EUR 545 |
Mut Express MultiS Fast Mutagenesis Kit V2 |
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| C215-01 | Vazyme | 10 rxn | EUR 92.66 |
Mut Express MultiS Fast Mutagenesis Kit V2 |
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| C215-01-10rxns | Vazyme | 10 rxns | EUR 95.95 |
Mut Express MultiS Fast Mutagenesis Kit V2 |
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| C215-02 | Vazyme | 25 rxn | EUR 194 |
Mut Express MultiS Fast Mutagenesis Kit V2 |
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| C215-02-25rxns | Vazyme | 25 rxns | EUR 200.89 |
Jade Express? Mix-Low ROX |
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| M1111-500 | Biovision | each | EUR 529.2 |
Star 1kb DNA Ladder Express |
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| BT10701 | Bioatlas | 500µl | EUR 96 |
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Description: High purity buffer for various PCR applications. |
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Staph Express Count Plate - PK50 |
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| FSA1042 | Scientific Laboratory Supplies | PK50 | EUR 132.3 |
Cryo Express Dry Shipper (CX100) |
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| TW-CX100 | MiTeGen | 1 SHIPPER | EUR 1265 |
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Description: Cryo Express Dry Shipper (CX100) |
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Express Coomassie Stain G-250 |
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| 40340020-1 | Bio-WORLD | 1 L | EUR 122.35 |
Express Coomassie Stain G-250 |
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| 40340020-2 | Bio-WORLD | 500 mL | EUR 83.47 |
Express Coomassie Stain, R-250 |
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| 40340024-1 | Bio-WORLD | 100 mL | EUR 64.28 |
Staph Express Count Plate - PK500 |
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| FSA1040 | Scientific Laboratory Supplies | PK500 | EUR 931.5 |
MILLIPAK EXPRESS 40 FILTER - EACH |
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| MPGP04001SIG | Scientific Laboratory Supplies | EACH | EUR 293.76 |
Star 100 bp DNA Ladder Express |
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| BA01302 | Bioatlas | 500µl | EUR 96 |
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Description: High purity buffer for various PCR applications. |
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Brilliant Sodium Express - No wash sodium assay kit |
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| 9000-100 | ION Biosciences | 100 Plate | EUR 3079.55 |
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Description: No wash sodium assay, 10 plates. Total assay solution for plate-based, high-throughput measurements of intracellular sodium flux. Also endpoint assay compatible.Excitation: 525 nm | Emission: 545 nmION’s Brilliant Sodium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes in intracellular Na⁺ mediated through a wide-variety of plasma membrane and intracellular sodium channels and transporters. In multi-well, plate-based formats, the Brilliant Sodium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Na⁺. ION’s Brilliant Sodium Assay provides all the reagents necessary for use as a wash or no-wash assay with adherent or non-adherent cells. The optional use of a probenecid solution and an extracellular background masking solution (TRS) offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Na⁺ indicators (e.g. Chinese Hamster Ovary, CHO cells) and when performing assays in complete, serum-containing cell culture medium is desired.ION’s Brilliant Sodium Assay is compatible with fluorescence microscopes, flow cytometers, and plate readers capable of detecting fluorescein or more optimally, yellow fluorescent protein (YFP). |
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Brilliant Calcium Express - No wash calcium assay kit |
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| 10000-100 | ION Biosciences | 100 Plate | EUR 2608.65 |
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Description: No wash calcium assay, 100 plates. Total assay solution for plate-based, high-throughput measurements of intracellular calcium flux. Excitation: 490 nm | Emission: 515 nmION Biosciences Brilliant Calcium Assay is a total assay solution for multi-well plate-based, high-throughput measurements of changes intracellular Ca2+ mediated through a wide-variety of plasma membrane and intracellular calcium channels and transporters. The ION Biosciences Brilliant Calcium Assay is also useful for investigating numerous effectors of ion channels and transporters including G protein-coupled receptors, lipid kinases and protein kinases. In multi-well, plate-based formats, the Brilliant Calcium Assay can be used to discover and characterize the effects of many tens-of-thousands of compounds and environmental factors on effectors of intracellular Ca2+. For the last 25 years, fluorescence-based measures of Ca2+ flux have brought about the discovery of small-molecule modulators of a host of Ion channels, transporters, GPCRS and other targets of interest for both drug discovery and basic research. ION Brilliant Calcium Express provides all the reagents necessary for use as a washed or no-wash assay with adherent or non-adherent cells. The optional use of a probenecid solution and an extracellular background masking solution offers the ultimate in compatibility for cells types which are difficult to load with fluorescent Ca2+ indicators (e.g. Chinese Hamster Ovary, CHO cells) and when performing assays in complete, serum-containing cell culture medium is desired. |
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Novel Express qPCR System-ROX I |
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| MBS402062-100Reactions | MyBiosource | 100Reactions | EUR 220 |
Novel Express qPCR System-ROX I |
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| MBS402062-20Reactions | MyBiosource | 20Reactions | EUR 170 |
Novel Express qPCR System - ROX Free |
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| MBS402064-100Reactions | MyBiosource | 100Reactions | EUR 220 |
We confirmed that the T-DNAs have been transmitted to the following technology via the segregation of GFP alerts within the T1 technology. These outcomes present that many Oryza species could be remodeled by utilizing modified immature-embryo strategies. It will speed up the usage of wild Oryza accessions in molecular genetic analyses and molecular breeding.