
The elevated utilization of high-throughput applied sciences in most cancers analysis, together with genetic and drug screens, generates massive units of candidate targets that should be functionally validated for his or her roles in tumor growth. Thus, dependable and strong in vivo mannequin methods are wanted to carry out reverse genetic experiments. Ideally, these fashions ought to enable for a conditional silencing of the goal and an unambiguous identification of engineered most cancers cells. Right here, we current a platform consisting of: (i) t(8;21) and t(15;17) pushed acute myeloid leukemia (AML) transgenic mice with constitutive expression of inexperienced fluorescent protein (GFP) and inducible expression of Cre recombinase, and (ii) REX, a modified pSico lentiviral vector for inducible shRNA expression and crimson fluorescent protein (RFP) as a range marker.
On this system, leukemic cells from transgenic mice are transduced with REX, circulate sorted, and transplanted into syngeneic hosts. Gene interference is induced in established tumors by tamoxifen remedy. Twin-color cell fluorescence guides the in vivo identification of shRNA interfered AML cells, monitoring engraftment and illness development. We examined the platform by inducing knockdown of Zeb2, a gene upregulated by AML1-ETO and PML-RARα oncogenes in pre-leukemic hematopoietic stem cell compartment, and noticed a big delay in leukemia onset. This proves the ability and utility of the platform and confirms Zeb2 contribution to the pathogenesis of AML. Transgenes are susceptible to progressive silencing as a result of their construction, copy quantity, and genomic location. In C. elegans, repressive mechanisms are significantly robust within the germline with nearly totally penetrant transgene silencing in easy extrachromosomal arrays and frequent silencing of single-copy transgene insertions.
A category of non-coding DNA, Periodic An/Tn Clusters (PATCs) can forestall transgene-silencing in repressive chromatin or from small interfering RNAs (piRNAs). Right here, we describe design guidelines (codon-optimization, intron and PATC inclusion, elevated temperature (25 °C), and vector spine elimination) for environment friendly germline expression from arrays in wildtype animals.
BDNF Overexpression Will increase Striatal D3 Receptor Degree at Striatal Neurons and Exacerbates D1-Receptor Agonist-Induced Dyskinesia

A Novel Platform to Test In Vivo Single Gene Dependencies in t(8,21) and t(15,17) AML Confirms Zeb2 as Leukemia Target
Agrobacterium-Mediated Genetic Transformation of Wild Oryza Species Utilizing Immature Embryos
Express Cloning Checker Kit I: Yellow Solution |
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K5011200-2 | Biochain | 1 ml | EUR 130.8 |
Mut Express II Fast Mutagenesis Kit V2 |
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C214-01 | Vazyme | 10 rxn | EUR 198 |
Mut Express II Fast Mutagenesis Kit V2 |
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C214-02 | Vazyme | 25 rxn | EUR 283.2 |
Human Drug Detoxication II |
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HDTX-II | Real Time Primers | 1 set | EUR 774 |
Human Kinase Library II |
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HKIN-II | Real Time Primers | 1 set | EUR 540 |
Mouse Cytokine Primer Library II |
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MCA-II | Real Time Primers | 1 set | EUR 540 |
Human Cytokine Primer Library II |
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HCA-II | Real Time Primers | 1 set | EUR 540 |
Rat Cytokine Primer Library II |
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RCA-II | Real Time Primers | 1 set | EUR 657.6 |
Mouse DNA Repair Primer Library II |
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MDRL-II | Real Time Primers | 1 set | EUR 774 |
Human DNA Repair Primer Library II |
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HDRL-II | Real Time Primers | 1 set | EUR 657.6 |
Human Colon Cancer Primer Library II |
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HCCP-II | Real Time Primers | 1 set | EUR 657.6 |
Human Cancer Driver Gene II Primer Library |
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HCDG-II | Real Time Primers | 1 set | EUR 774 |
Human Interferon Type II Signaling Primer Library |
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HIFN-II | Real Time Primers | 1 set | EUR 657.6 |
PrecisionX Multiplex gRNA Cloning Kit |
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CAS9-GRNA-KIT | SBI | 10 rxn | EUR 534 |
Checker Plus pH Tester |
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HI98100 | Scientific Laboratory Supplies | EACH | EUR 55.75 |
Jade Express? Mix |
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M1109-500 | Biovision | EUR 529.2 |
Staph Express Disk |
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FSA1044 | Scientific Laboratory Supplies | PK100 | EUR 117.42 |
Staph Express Disk |
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FSA1046 | Scientific Laboratory Supplies | PK20 | EUR 40.47 |
Express CRISPR sgRNA Synthesis Kit |
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K1253-25 | Biovision | EUR 639.6 |
SLS Lab Basics pH Checker |
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PHM9500 | Scientific Laboratory Supplies | EACH | EUR 55.29 |
ClonExpress II One Step Cloning Kit |
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C112-01 | Vazyme | 25 rxn | EUR 202.8 |
ClonExpress II One Step Cloning Kit |
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C112-02 | Vazyme | 50 rxn | EUR 259.2 |
ToxOut? Express Endofree Plasmid Midi Kit |
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K1335-10 | Biovision | EUR 444 |
ToxOut? Express Endofree Plasmid Midi Kit |
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K1335-2 | Biovision | EUR 280.8 |
ToxOut? Express Endofree Plasmid Midi Kit |
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K1335-25 | Biovision | EUR 836.4 |
ToxOut? Express Endofree Plasmid Maxi Kit |
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K1336-10 | Biovision | EUR 574.8 |
ToxOut? Express Endofree Plasmid Maxi Kit |
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K1336-2 | Biovision | EUR 314.4 |
ToxOut? Express Endofree Plasmid Maxi Kit |
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K1336-25 | Biovision | EUR 1032 |
Express Plasmid Midiprep Kit (25 min) |
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K1323-10 | Biovision | EUR 411.6 |
Express Plasmid Midiprep Kit (25 min) |
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K1323-2 | Biovision | EUR 314.4 |
Express Plasmid Midiprep Kit (25 min) |
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K1323-25 | Biovision | EUR 561.6 |
Express Plasmid Maxiprep Kit (25 min) |
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K1324-10 | Biovision | EUR 510 |
Express Plasmid Maxiprep Kit (25 min) |
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K1324-2 | Biovision | EUR 339.6 |
Express Plasmid Maxiprep Kit (25 min) |
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K1324-25 | Biovision | EUR 732 |
Jade Express? Mix-iCycler |
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M1110-500 | Biovision | EUR 529.2 |
Jade Express? Mix-ROX |
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M1112-500 | Biovision | EUR 529.2 |
pIRES2- DsRed- Express Plasmid |
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PVT1229 | Lifescience Market | 2 ug | EUR 289.2 |
pDsRed- Express- C1 Plasmid |
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PVT1236 | Lifescience Market | 2 ug | EUR 319.2 |
MILLIPAK EXPRESS 40 FILTER |
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MPGP04001SIG | Scientific Laboratory Supplies | EACH | EUR 194.94 |
Staph Express Count Plate |
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FSA1040 | Scientific Laboratory Supplies | PK500 | EUR 673.74 |
Staph Express Count Plate |
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FSA1042 | Scientific Laboratory Supplies | PK50 | EUR 94.62 |
Mut Express MultiS Fast Mutagenesis Kit V2 |
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C215-01 | Vazyme | 10 rxn | EUR 297.6 |
Mut Express MultiS Fast Mutagenesis Kit V2 |
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C215-02 | Vazyme | 25 rxn | EUR 490.8 |
Star 1kb DNA Ladder Express |
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BT10701 | Bioatlas | 500µl | EUR 96 |
Description: High purity buffer for various PCR applications. |
Jade Express? Mix-Low ROX |
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M1111-500 | Biovision | EUR 529.2 |
Cryo Express Dry Shipper (CX100) |
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TW-CX100 | MiTeGen | 1 SHIPPER | EUR 1380 |
Description: Cryo Express Dry Shipper (CX100) |
Frit Kit |
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FRIT-KIT | Next Advance | 1each | EUR 148.8 |
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
RapidSeq High Yield Directional mRNA Sample Prep Kit - With Aligner 13-24 |
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KS073012-II | Biochain | 1 Kit | EUR 1370.4 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
RapidSeq High Yield Small RNA Sample Prep Kit - With Aligner 13-24 |
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KS074012-II | Biochain | 1 Kit | EUR 1225.2 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti rabbit secondary antibody |
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K3171120-II | Biochain | 1200 cm2 | EUR 295.2 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti rabbit secondary antibody |
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K3171250-II | Biochain | 2500 cm2 | EUR 372 |
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
Column Packing Kit |
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PACK-KIT | Next Advance | 1pack | EUR 1242 |
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
PCR Mycoplasma Detection Kit |
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M034-Kit | TOKU-E | Kit | EUR 319.2 |
Star 100 bp DNA Ladder Express |
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BA01302 | Bioatlas | 500µl | EUR 96 |
Description: High purity buffer for various PCR applications. |
BrightGreen Express 2X qPCR MasterMix-iCycler |
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MasterMix-EC | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
BrightGreen Express 2X qPCR MasterMix-ROX |
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MasterMix-ER | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
Millipak Express 20 Filter (1/box) |
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MPGP02001 | Scientific Laboratory Supplies | EACH | EUR 208.85 |
Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
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CAS400A-KIT | SBI | 1 kit (10 rxn) | EUR 1332 |
Pesticide Kit Containing All 10 Multi-Compound Mixes |
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SPXPR-KIT | Scientific Laboratory Supplies | 1ML | EUR 1923.18 |
CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV100PA-KIT | SBI | 1 Kit | EUR 1358.4 |
CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV105PA-KIT | SBI | 1 Kit | EUR 1358.4 |
MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV120PA-KIT | SBI | 1 Kit | EUR 1358.4 |
MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV125PA-KIT | SBI | 1 Kit | EUR 1358.4 |
Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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CAS700A-KIT | SBI | 10 rxn | EUR 1358.4 |
Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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CAS720A-KIT | SBI | 10 rxn | EUR 1358.4 |
Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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CAS740A-KIT | SBI | 10 rxn | EUR 1358.4 |
T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
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CAS510A-KIT | SBI | 1 Kit | EUR 966 |
Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV200PA-KIT | SBI | 1 Kit | EUR 1358.4 |
Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV205PA-KIT | SBI | 1 Kit | EUR 1358.4 |
Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV220PA-KIT | SBI | 1 Kit | EUR 1358.4 |
Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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CASLV225PA-KIT | SBI | 1 Kit | EUR 1358.4 |
Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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CAS750A-KIT | SBI | 10 rxn | EUR 1358.4 |
Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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CAS770A-KIT | SBI | 10 rxn | EUR 1358.4 |
Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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CAS790A-KIT | SBI | 10 rxn | EUR 1358.4 |
Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] |
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CAS9LIG-KIT | SBI | 1 Kit | EUR 183.6 |
Claritas PPT Grade Minerals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL |
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SPXMT-KIT | Scientific Laboratory Supplies | 30ML | EUR 237.12 |
PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
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PIN320A-KIT | SBI | 1 Kit | EUR 5929.2 |
Claritas PPT Grade Heavy Metals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL |
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SPXHM-KIT | Scientific Laboratory Supplies | 30ML | EUR 245.1 |
Aligner 13-24 |
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KS072012-II | Biochain | 1 Kit | EUR 494.4 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
BrightGreen Express 2X qPCR MasterMix-Low ROX |
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MasterMix-EL | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
BrightGreen Express 2X qPCR MasterMix-No Dye |
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MasterMix-ES | ABM | 4 x 1.25 ml for 500 reactions (20 ul) | EUR 168 |
PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
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PIN340iPS-KIT | SBI | 1 Kit | EUR 5929.2 |
AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
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GE620A-KIT | SBI | 1 kit | EUR 2558.4 |
AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
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GE622A-KIT | SBI | 1 kit | EUR 2558.4 |
AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
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GE624A-KIT | SBI | 1 kit | EUR 2558.4 |
Hanna Checker pH Tester HI 98103 with 0.1 pH Resolution |
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PHM4000 | Scientific Laboratory Supplies | EACH | EUR 61.56 |
vWF Acty. Kit |
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ABP-ACT-KIT | Abpcorp | 12 x 8 microwells | EUR 513.6 |
vWF Ant. Kit |
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ABP-TOT-KIT | Abpcorp | 12 x 8 microwells | EUR 472.8 |
hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9) |
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CAS620A-KIT | SBI | 1 kit | EUR 2582.4 |
Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression) |
PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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PIN300A-KIT | SBI | 1 Kit | EUR 3357.6 |
PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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PIN400A-KIT | SBI | 1 Kit | EUR 3357.6 |
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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PIN410A-KIT | SBI | 1 Kit | EUR 5202 |
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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PIN412A-KIT | SBI | 1 Kit | EUR 5202 |
Filtration System 47mm Complete Assembly |
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5809/KIT | Scientific Laboratory Supplies | EACH | EUR 427.69 |
Angiotensin II (Ang II) ELISA Kit |
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abx511338-96tests | Abbexa | 96 tests | EUR 801.6 |
mRNAExpress mRNA Synthesis kit (5 reactions) |
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MR-KIT-1 | SBI | 5 reactions | EUR 1382.4 |
42 Component Terpene Kit in Methanol |
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CAN-TERP-KIT | Scientific Laboratory Supplies | 1ML | EUR 287.28 |
Canadian Pesticide Kit Containing All 6 Mixes |
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CAN-CAN-KIT | Scientific Laboratory Supplies | 1ML | EUR 758.1 |
AxioCam Erc5s rev 2, w/Accessory Kit |
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ZV-Erc5sAC-Kit | MiTeGen | 1 KIT | EUR 1611 |
Description: AxioCam Erc5s rev 2, w/Accessory Kit- connection cable Y-USB 2.0.- HDMI cable to DVI-D- SD card- IR remote control |
Cryo Express Dry Shipper with replaceable absorbent Material (CXR100) |
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TW-CXR100 | MiTeGen | 1 SHIPPER | EUR 1380 |
Description: Cryo Express Dry Shipper with replaceable absorbent Material (CXR100) |
T1 Cloning Kit |
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20-abx098055 | Abbexa |
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T3 Cloning Kit |
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20-abx098057 | Abbexa |
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Blunt Cloning Kit |
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20-abx098059 | Abbexa |
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Blunt3 Cloning Kit |
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20-abx098061 | Abbexa |
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General Ang-II/ Angiotensin II ELISA Kit |
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E0086Ge | Sunlong | 1 Kit | EUR 675.6 |
Human ANG II(Angiotensin II) ELISA Kit |
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EH2627 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
Human MHC II/RLA II ELISA Kit |
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EHM0285 | Abclonal | 96Tests | EUR 625.2 |
Porcine MHC II/RLA II ELISA Kit |
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EPM0285 | Abclonal | 96Tests | EUR 625.2 |
Rat ANG II (Angiotensin II) ELISA Kit |
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ER1637 | FN Test | 96T | EUR 681.12 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml |
Rabbit ANG II(Angiotensin II) ELISA Kit |
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ERB0006 | FN Test | 96T | EUR 681.12 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rabbit;Sensitivity: 18.75pg/ml |
Mouse MHC II/RLA II ELISA Kit |
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EMM0285 | Abclonal | 96Tests | EUR 625.2 |
Rat MHC II/RLA II ELISA Kit |
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ERM0285 | Abclonal | 96Tests | EUR 625.2 |
Rabbit MHC II/RLA II ELISA Kit |
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ERTM0285 | Abclonal | 96Tests | EUR 625.2 |
Rabbit Angiotensin II (Ang II) ELISA Kit |
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20-abx155034 | Abbexa |
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Rat Angiotensin II (Ang II) ELISA Kit |
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20-abx155198 | Abbexa |
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Chicken Angiotensin II (Ang II) ELISA Kit |
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20-abx150002 | Abbexa |
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Cow Angiotensin II (Ang II) ELISA Kit |
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20-abx150070 | Abbexa |
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Horse Angiotensin II (Ang II) ELISA Kit |
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20-abx150439 | Abbexa |
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Human Angiotensin II (Ang II) ELISA Kit |
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20-abx150654 | Abbexa |
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Mouse Angiotensin II (Ang II) ELISA Kit |
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20-abx153637 | Abbexa |
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Rat Angiotensin II (Ang II) ELISA Kit |
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20-abx052349 | Abbexa |
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Human Angiotensin II (ANG II) CLIA Kit |
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abx195117-96tests | Abbexa | 96 tests | EUR 990 |