Background
Vectors based mostly on plant viruses are essential instruments for purposeful genomics, mobile biology, plant genome engineering and molecular farming. We beforehand reported on the development of PepGFPvector based mostly on pepino mosaic virus (PepMV) which expressed GFP effectively and stably in vegetation of its experimental host Nicotiana benthamiana, however not in its pure host tomato. We have now ready a brand new set of PepMV-based vectors with improved stability which can be in a position to specific a variety of reporter genes, helpful for each N. benthamiana and tomato.
Outcomes
We first examined PepGFPm1 and PepGFPm2, two variants of PepGFPvectors had improved GFP expression ranges and stability in N. benthamiana however not in tomato vegetation. Subsequent, we changed GFP by DsRed or mCherry within the new vectors PepDsRed and PepmCherry, respectively; whereas PepmCherry behaved equally to PepGFPm2, PepDsRed expressed the reporter gene effectively additionally in tomato vegetation. We then used PepGFPm2 and PepDsRed to review the PepMV localization in each N. benthamiana and tomato cells. Utilizing confocal laser scanning microscopy (CLSM), we noticed attribute fluorescent our bodies in PepMV-infected cells; these our bodies had a cytoplasmic localization and appeared in shut proximity to the cell nucleus. Already at Three days post-agroinoculation there have been fluorescent our bodies in virtually each cell of agroinoculated tissues of each hosts, and all the time one physique per cell.
When markers for the endoplasmic reticulum or the Golgi equipment had been co-expressed with PepGFPm2 or PepDsRed, a reorganisation of those organelles was noticed, with photos suggesting that each are intimately associated however not the principle constituents of the PepMV our bodies. Altogether, this set of information steered that the PepMV our bodies are much like the potato virus X (PVX) “X-bodies”, which have been described because the PVX viral replication complexes (VRCs). To finish the set of PepMV-based vectors, we constructed a vector expressing the BAR herbicide resistance gene, helpful for large susceptibility screenings.
Conclusions
We have now considerably expanded the PepMV device field by producing a set of recent vectors with improved stability and effectivity in each N. benthamiana and tomato vegetation. By utilizing two of those vectors, we now have described attribute mobile our bodies induced by PepMV an infection; these our bodies are doubtless the PepMV VRCs.
A Artificial Plasmid Toolkit for Shewanella oneidensis MR-1.
Shewanella oneidensis MR-1 is a platform microorganism for understanding extracellular electron switch (EET) with a completely sequenced and annotated genome. Compared to different mannequin microorganisms equivalent to Escherichia coli, the obtainable plasmid elements (equivalent to promoters and replicons) are usually not adequate to conveniently and rapidly fine-tune the expression of a number of genes in S. oneidensis MR-1. Right here, we constructed and characterised a plasmid toolkit that comprises a set of expression vectors with a mixture of promoters, replicons, antibiotic resistance genes, and an RK2 origin of switch (oriT) cassette, during which every ingredient may be simply modified by mounted restriction enzyme websites. The expression cassette can also be suitable with BioBrick artificial biology requirements. Utilizing inexperienced fluorescent protein (GFP) as a reporter, we examined and quantified the energy of promoters.
The copy variety of totally different replicons was additionally measured by real-time quantitative PCR. We additional remodeled two suitable plasmids with totally different antibiotic resistance genes into the recombinant S. oneidensis MR-1, enabling management over the expression of two totally different fluorescent proteins. This plasmid toolkit was additional used for overexpression of the MtrCAB porin-c-type cytochrome complicated within the S. oneidensis ΔmtrA pressure. Tungsten trioxide (WO3) discount and microbial gasoline cell (MFC) assays revealed that the EET effectivity was improved most importantly when MtrCAB was expressed at a average stage, thus demonstrating the utility of the plasmid toolkit within the EET regulation in S. oneidensis. The plasmid toolkit developed on this examine is helpful for fast and handy fine-tuning of gene expression and enhances the power to genetically manipulate S. oneidensis MR-1.
Second generation of pepino mosaic virus vectors: improved stability in tomato and a wide range of reporter genes.
Bodily decoupling of XylS/Pm regulatory parts and conditional proteolysis allow exact management of gene expression in Pseudomonas putida.
Many of the gene expression techniques obtainable for Gram-negative micro organism are stricken by comparatively excessive ranges of basal (i.e. leaky) expression of the goal gene(s). This prevalence impacts the system dynamics, finally lowering the output and productiveness of engineered pathways and artificial circuits.
With the intention to circumvent this downside, we now have designed a novel expression system based mostly on the well-known XylS/Pm transcriptional regulator/promoter pair from the soil bacterium Pseudomonas putida mt-2, during which the important thing purposeful parts are bodily decoupled. By integrating the xylS gene into the chromosome of the platform pressure KT2440, whereas putting the Pm promoter right into a set of ordinary plasmid vectors, the inducibility of the system (i.e. the output distinction between the induced and uninduced state) improved as much as 170-fold. We additional mixed this modular system with an additional layer of post-translational management by the use of conditional proteolysis.
Blunt Cloning Kit |
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| 20-abx098059 | Abbexa |
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Blunt3 Cloning Kit |
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| 20-abx098061 | Abbexa |
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XG cloning plasmid |
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| CSB-CL026190HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the XG gene. |
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XK cloning plasmid |
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| CSB-CL026198HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the XK gene. |
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GK cloning plasmid |
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| CSB-CL300097HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GK gene. |
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IK cloning plasmid |
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| CSB-CL613381HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the IK gene. |
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C5 cloning plasmid |
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| CSB-CL003995HU1-10ug | Cusabio | 10ug | EUR 3324 |
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Description: A cloning plasmid for the C5 gene. |
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C5 cloning plasmid |
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| CSB-CL003995HU2-10ug | Cusabio | 10ug | EUR 3324 |
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Description: A cloning plasmid for the C5 gene. |
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C6 cloning plasmid |
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| CSB-CL004036HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the C6 gene. |
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C7 cloning plasmid |
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| CSB-CL004145HU-10ug | Cusabio | 10ug | EUR 669.6 |
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Description: A cloning plasmid for the C7 gene. |
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C9 cloning plasmid |
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| CSB-CL004259HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the C9 gene. |
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CS cloning plasmid |
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| CSB-CL006031HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CS gene. |
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CS cloning plasmid |
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| CSB-CL006031HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CS gene. |
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F2 cloning plasmid |
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| CSB-CL007923HU-10ug | Cusabio | 10ug | EUR 759.6 |
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Description: A cloning plasmid for the F2 gene. |
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F3 cloning plasmid |
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| CSB-CL007928HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the F3 gene. |
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F7 cloning plasmid |
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| CSB-CL007930HU-10ug | Cusabio | 10ug | EUR 579.6 |
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Description: A cloning plasmid for the F7 gene. |
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F8 cloning plasmid |
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| CSB-CL007932HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the F8 gene. |
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F9 cloning plasmid |
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| CSB-CL007936HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the F9 gene. |
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FH cloning plasmid |
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| CSB-CL008659HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the FH gene. |
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GC cloning plasmid |
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| CSB-CL009306HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GC gene. |
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PC cloning plasmid |
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| CSB-CL017511HU-10ug | Cusabio | 10ug | EUR 1502.4 |
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Description: A cloning plasmid for the PC gene. |
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HP cloning plasmid |
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| CSB-CL010691HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the HP gene. |
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HP cloning plasmid |
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| CSB-CL010691HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the HP gene. |
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MB cloning plasmid |
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| CSB-CL013529HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the MB gene. |
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MB cloning plasmid |
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| CSB-CL013529HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the MB gene. |
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HYI cloning plasmid |
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| CSB-CL719395HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the HYI gene. |
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HYI cloning plasmid |
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| CSB-CL719395HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the HYI gene. |
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SHE cloning plasmid |
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| CSB-CL730142HU-10ug | Cusabio | 10ug | EUR 632.4 |
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Description: A cloning plasmid for the SHE gene. |
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GK5 cloning plasmid |
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| CSB-CL744414HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GK5 gene. |
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EMB cloning plasmid |
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| CSB-CL754239HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the EMB gene. |
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IYD cloning plasmid |
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| CSB-CL754272HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the IYD gene. |
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KLB cloning plasmid |
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| CSB-CL769815HU-10ug | Cusabio | 10ug | EUR 1346.4 |
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Description: A cloning plasmid for the KLB gene. |
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PXK cloning plasmid |
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| CSB-CL770363HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the PXK gene. |
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RD3 cloning plasmid |
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| CSB-CL800101HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the RD3 gene. |
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OAF cloning plasmid |
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| CSB-CL803119HU-10ug | Cusabio | 10ug | EUR 406.8 |
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Description: A cloning plasmid for the OAF gene. |
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NRM cloning plasmid |
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| CSB-CL816902HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the NRM gene. |
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MKX cloning plasmid |
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| CSB-CL818257HU-10ug | Cusabio | 10ug | EUR 487.2 |
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Description: A cloning plasmid for the MKX gene. |
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AEN cloning plasmid |
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| CSB-CL819883HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the AEN gene. |
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AEN cloning plasmid |
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| CSB-CL819883HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the AEN gene. |
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AEN cloning plasmid |
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| CSB-CL819883HU3-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the AEN gene. |
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PBK cloning plasmid |
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| CSB-CL822241HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the PBK gene. |
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TTL cloning plasmid |
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| CSB-CL822788HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the TTL gene. |
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RFK cloning plasmid |
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| CSB-CL836167HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the RFK gene. |
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ACD cloning plasmid |
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| CSB-CL839289HU-10ug | Cusabio | 10ug | EUR 680.4 |
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Description: A cloning plasmid for the ACD gene. |
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BAD cloning plasmid |
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| CSB-CL842172HU-10ug | Cusabio | 10ug | EUR 301.2 |
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Description: A cloning plasmid for the BAD gene. |
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HGD cloning plasmid |
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| CSB-CL842354HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the HGD gene. |
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QKI cloning plasmid |
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| CSB-CL842739HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the QKI gene. |
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BBX cloning plasmid |
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| CSB-CL845172HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the BBX gene. |
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FUK cloning plasmid |
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| CSB-CL847604HU-10ug | Cusabio | 10ug | EUR 1131.6 |
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Description: A cloning plasmid for the FUK gene. |
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TNF cloning plasmid |
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| CSB-CL023955HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the TNF gene. |
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TOX cloning plasmid |
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| CSB-CL024073HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the TOX gene. |
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TPO cloning plasmid |
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| CSB-CL024112HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the TPO gene. |
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TRH cloning plasmid |
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| CSB-CL024439HU-10ug | Cusabio | 10ug | EUR 376.8 |
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Description: A cloning plasmid for the TRH gene. |
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TRO cloning plasmid |
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| CSB-CL025069HU-10ug | Cusabio | 10ug | EUR 818.4 |
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Description: A cloning plasmid for the TRO gene. |
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TTK cloning plasmid |
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| CSB-CL025251HU1-10ug | Cusabio | 10ug | EUR 669.6 |
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Description: A cloning plasmid for the TTK gene. |
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TTK cloning plasmid |
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| CSB-CL025251HU2-10ug | Cusabio | 10ug | EUR 669.6 |
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Description: A cloning plasmid for the TTK gene. |
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TTR cloning plasmid |
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| CSB-CL025270HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the TTR gene. |
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TYR cloning plasmid |
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| CSB-CL025394HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the TYR gene. |
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UBD cloning plasmid |
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| CSB-CL025435HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the UBD gene. |
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UCN cloning plasmid |
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| CSB-CL025551HU-10ug | Cusabio | 10ug | EUR 258 |
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Description: A cloning plasmid for the UCN gene. |
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UNG cloning plasmid |
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| CSB-CL025641HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the UNG gene. |
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UTY cloning plasmid |
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| CSB-CL025775HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the UTY gene. |
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VCL cloning plasmid |
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| CSB-CL025812HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the VCL gene. |
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VCP cloning plasmid |
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| CSB-CL025813HU-10ug | Cusabio | 10ug | EUR 944.4 |
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Description: A cloning plasmid for the VCP gene. |
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VCY cloning plasmid |
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| CSB-CL025819HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the VCY gene. |
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VDR cloning plasmid |
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| CSB-CL025832HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the VDR gene. |
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VHL cloning plasmid |
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| CSB-CL025852HU-10ug | Cusabio | 10ug | EUR 306 |
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Description: A cloning plasmid for the VHL gene. |
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VIM cloning plasmid |
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| CSB-CL025857HU-10ug | Cusabio | 10ug | EUR 602.4 |
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Description: A cloning plasmid for the VIM gene. |
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VIP cloning plasmid |
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| CSB-CL025858HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the VIP gene. |
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VIT cloning plasmid |
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| CSB-CL025862HU-10ug | Cusabio | 10ug | EUR 795.6 |
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Description: A cloning plasmid for the VIT gene. |
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VTN cloning plasmid |
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| CSB-CL025944HU-10ug | Cusabio | 10ug | EUR 613.2 |
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Description: A cloning plasmid for the VTN gene. |
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VWF cloning plasmid |
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| CSB-CL025960HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the VWF gene. |
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WAS cloning plasmid |
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| CSB-CL025967HU-10ug | Cusabio | 10ug | EUR 638.4 |
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Description: A cloning plasmid for the WAS gene. |
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WRB cloning plasmid |
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| CSB-CL026148HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the WRB gene. |
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WT1 cloning plasmid |
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| CSB-CL026158HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the WT1 gene. |
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XPA cloning plasmid |
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| CSB-CL026216HU-10ug | Cusabio | 10ug | EUR 406.8 |
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Description: A cloning plasmid for the XPA gene. |
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YY1 cloning plasmid |
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| CSB-CL026297HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the YY1 gene. |
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ZFY cloning plasmid |
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| CSB-CL026468HU-10ug | Cusabio | 10ug | EUR 939.6 |
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Description: A cloning plasmid for the ZFY gene. |
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ZYX cloning plasmid |
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| CSB-CL027165HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the ZYX gene. |
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UBB cloning plasmid |
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| CSB-CL316022HU1-10ug | Cusabio | 10ug | EUR 363.6 |
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Description: A cloning plasmid for the UBB gene. |
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UBB cloning plasmid |
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| CSB-CL316022HU2-10ug | Cusabio | 10ug | EUR 363.6 |
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Description: A cloning plasmid for the UBB gene. |
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UBC cloning plasmid |
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| CSB-CL316735HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the UBC gene. |
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OCM cloning plasmid |
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| CSB-CL317613HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the OCM gene. |
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CD6 cloning plasmid |
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| CSB-CL326953HU-10ug | Cusabio | 10ug | EUR 805.2 |
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Description: A cloning plasmid for the CD6 gene. |
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AXL cloning plasmid |
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| CSB-CL326981HU-10ug | Cusabio | 10ug | EUR 1033.2 |
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Description: A cloning plasmid for the AXL gene. |
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ADK cloning plasmid |
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| CSB-CL346232HU-10ug | Cusabio | 10ug | EUR 481.2 |
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Description: A cloning plasmid for the ADK gene. |
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ITK cloning plasmid |
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| CSB-CL600145HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the ITK gene. |
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TEF cloning plasmid |
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| CSB-CL608815HU-10ug | Cusabio | 10ug | EUR 436.8 |
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Description: A cloning plasmid for the TEF gene. |
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SLA cloning plasmid |
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| CSB-CL613387HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the SLA gene. |
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OS9 cloning plasmid |
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| CSB-CL613402HU1-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the OS9 gene. |
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OS9 cloning plasmid |
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| CSB-CL613402HU2-10ug | Cusabio | 10ug | EUR 750 |
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Description: A cloning plasmid for the OS9 gene. |
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LBR cloning plasmid |
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| CSB-CL613520HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the LBR gene. |
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FXN cloning plasmid |
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| CSB-CL613687HU-10ug | Cusabio | 10ug | EUR 344.4 |
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Description: A cloning plasmid for the FXN gene. |
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MIA cloning plasmid |
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| CSB-CL613694HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the MIA gene. |
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FAP cloning plasmid |
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| CSB-CL614259HU-10ug | Cusabio | 10ug | EUR 568.8 |
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Description: A cloning plasmid for the FAP gene. |
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HLX cloning plasmid |
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| CSB-CL614810HU-10ug | Cusabio | 10ug | EUR 624 |
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Description: A cloning plasmid for the HLX gene. |
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MMD cloning plasmid |
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| CSB-CL614894HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the MMD gene. |
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CA9 cloning plasmid |
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| CSB-CL614990HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CA9 gene. |
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BIK cloning plasmid |
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| CSB-CL615677HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the BIK gene. |
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NNT cloning plasmid |
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| CSB-CL615685HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the NNT gene. |
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NNT cloning plasmid |
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| CSB-CL615685HU2-10ug | Cusabio | 10ug | EUR 1396.8 |
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Description: A cloning plasmid for the NNT gene. |
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NMI cloning plasmid |
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| CSB-CL618768HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the NMI gene. |
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NMI cloning plasmid |
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| CSB-CL618768HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the NMI gene. |
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ATM cloning plasmid |
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| CSB-CL618770HU1-10ug | Cusabio | 10ug | EUR 246 |
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Description: A cloning plasmid for the ATM gene. |
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ATM cloning plasmid |
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| CSB-CL618770HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the ATM gene. |
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MOG cloning plasmid |
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| CSB-CL619083HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the MOG gene. |
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ILK cloning plasmid |
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| CSB-CL619764HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the ILK gene. |
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AUH cloning plasmid |
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| CSB-CL621698HU-10ug | Cusabio | 10ug | EUR 445.2 |
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Description: A cloning plasmid for the AUH gene. |
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IHH cloning plasmid |
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| CSB-CL622779HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the IHH gene. |
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RLF cloning plasmid |
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| CSB-CL623786HU-10ug | Cusabio | 10ug | EUR 3324 |
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Description: A cloning plasmid for the RLF gene. |
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TDG cloning plasmid |
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| CSB-CL623816HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the TDG gene. |
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C1D cloning plasmid |
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| CSB-CL623834HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the C1D gene. |
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C1D cloning plasmid |
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| CSB-CL623834HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the C1D gene. |
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EBP cloning plasmid |
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| CSB-CL624016HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the EBP gene. |
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HFE cloning plasmid |
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| CSB-CL653744HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the HFE gene. |
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DAK cloning plasmid |
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| CSB-CL659468HU-10ug | Cusabio | 10ug | EUR 712.8 |
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Description: A cloning plasmid for the DAK gene. |
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DUT cloning plasmid |
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| CSB-CL662062HU1-10ug | Cusabio | 10ug | EUR 297.6 |
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Description: A cloning plasmid for the DUT gene. |
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DUT cloning plasmid |
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| CSB-CL662062HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the DUT gene. |
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SP6 cloning plasmid |
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| CSB-CL668784HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the SP6 gene. |
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LBH cloning plasmid |
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| CSB-CL700659HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the LBH gene. |
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LBH cloning plasmid |
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| CSB-CL700659HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the LBH gene. |
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AGK cloning plasmid |
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| CSB-CL706634HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the AGK gene. |
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AGK cloning plasmid |
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| CSB-CL706634HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the AGK gene. |
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ABO cloning plasmid |
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| CSB-CL001110HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the ABO gene. |
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ABO cloning plasmid |
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| CSB-CL001110HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the ABO gene. |
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ADA cloning plasmid |
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| CSB-CL001268HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the ADA gene. |
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ADC cloning plasmid |
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| CSB-CL001330HU-10ug | Cusabio | 10ug | EUR 596.4 |
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Description: A cloning plasmid for the ADC gene. |
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On this setup, the goal gene is tagged with an artificial motif dictating protein degradation. When the system options had been characterised utilizing the monomeric superfolder GFP as a mannequin protein, the basal ranges of fluorescence had been introduced right down to zero (i.e. under the restrict of detection). In all, these novel expression techniques represent another device to altogether suppress leaky gene expression, and they are often simply tailored to different vector codecs and plugged-in into totally different Gram-negative bacterial species on the person’s will.