Background
Vectors based mostly on plant viruses are essential instruments for purposeful genomics, mobile biology, plant genome engineering and molecular farming. We beforehand reported on the development of PepGFPvector based mostly on pepino mosaic virus (PepMV) which expressed GFP effectively and stably in vegetation of its experimental host Nicotiana benthamiana, however not in its pure host tomato. We have now ready a brand new set of PepMV-based vectors with improved stability which can be in a position to specific a variety of reporter genes, helpful for each N. benthamiana and tomato.
Outcomes
We first examined PepGFPm1 and PepGFPm2, two variants of PepGFPvectors had improved GFP expression ranges and stability in N. benthamiana however not in tomato vegetation. Subsequent, we changed GFP by DsRed or mCherry within the new vectors PepDsRed and PepmCherry, respectively; whereas PepmCherry behaved equally to PepGFPm2, PepDsRed expressed the reporter gene effectively additionally in tomato vegetation. We then used PepGFPm2 and PepDsRed to review the PepMV localization in each N. benthamiana and tomato cells. Utilizing confocal laser scanning microscopy (CLSM), we noticed attribute fluorescent our bodies in PepMV-infected cells; these our bodies had a cytoplasmic localization and appeared in shut proximity to the cell nucleus. Already at Three days post-agroinoculation there have been fluorescent our bodies in virtually each cell of agroinoculated tissues of each hosts, and all the time one physique per cell.
When markers for the endoplasmic reticulum or the Golgi equipment had been co-expressed with PepGFPm2 or PepDsRed, a reorganisation of those organelles was noticed, with photos suggesting that each are intimately associated however not the principle constituents of the PepMV our bodies. Altogether, this set of information steered that the PepMV our bodies are much like the potato virus X (PVX) “X-bodies”, which have been described because the PVX viral replication complexes (VRCs). To finish the set of PepMV-based vectors, we constructed a vector expressing the BAR herbicide resistance gene, helpful for large susceptibility screenings.
Conclusions
We have now considerably expanded the PepMV device field by producing a set of recent vectors with improved stability and effectivity in each N. benthamiana and tomato vegetation. By utilizing two of those vectors, we now have described attribute mobile our bodies induced by PepMV an infection; these our bodies are doubtless the PepMV VRCs.
A Artificial Plasmid Toolkit for Shewanella oneidensis MR-1.
Shewanella oneidensis MR-1 is a platform microorganism for understanding extracellular electron switch (EET) with a completely sequenced and annotated genome. Compared to different mannequin microorganisms equivalent to Escherichia coli, the obtainable plasmid elements (equivalent to promoters and replicons) are usually not adequate to conveniently and rapidly fine-tune the expression of a number of genes in S. oneidensis MR-1. Right here, we constructed and characterised a plasmid toolkit that comprises a set of expression vectors with a mixture of promoters, replicons, antibiotic resistance genes, and an RK2 origin of switch (oriT) cassette, during which every ingredient may be simply modified by mounted restriction enzyme websites. The expression cassette can also be suitable with BioBrick artificial biology requirements. Utilizing inexperienced fluorescent protein (GFP) as a reporter, we examined and quantified the energy of promoters.
The copy variety of totally different replicons was additionally measured by real-time quantitative PCR. We additional remodeled two suitable plasmids with totally different antibiotic resistance genes into the recombinant S. oneidensis MR-1, enabling management over the expression of two totally different fluorescent proteins. This plasmid toolkit was additional used for overexpression of the MtrCAB porin-c-type cytochrome complicated within the S. oneidensis ΔmtrA pressure. Tungsten trioxide (WO3) discount and microbial gasoline cell (MFC) assays revealed that the EET effectivity was improved most importantly when MtrCAB was expressed at a average stage, thus demonstrating the utility of the plasmid toolkit within the EET regulation in S. oneidensis. The plasmid toolkit developed on this examine is helpful for fast and handy fine-tuning of gene expression and enhances the power to genetically manipulate S. oneidensis MR-1.
Second generation of pepino mosaic virus vectors: improved stability in tomato and a wide range of reporter genes.
Bodily decoupling of XylS/Pm regulatory parts and conditional proteolysis allow exact management of gene expression in Pseudomonas putida.
Many of the gene expression techniques obtainable for Gram-negative micro organism are stricken by comparatively excessive ranges of basal (i.e. leaky) expression of the goal gene(s). This prevalence impacts the system dynamics, finally lowering the output and productiveness of engineered pathways and artificial circuits.
With the intention to circumvent this downside, we now have designed a novel expression system based mostly on the well-known XylS/Pm transcriptional regulator/promoter pair from the soil bacterium Pseudomonas putida mt-2, during which the important thing purposeful parts are bodily decoupled. By integrating the xylS gene into the chromosome of the platform pressure KT2440, whereas putting the Pm promoter right into a set of ordinary plasmid vectors, the inducibility of the system (i.e. the output distinction between the induced and uninduced state) improved as much as 170-fold. We additional mixed this modular system with an additional layer of post-translational management by the use of conditional proteolysis.
Cloning Optimizer |
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| MBS4156417-5x02mL | MyBiosource | 5x0.2mL | EUR 795 |
T1 Cloning Kit |
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| abx098055-100l | Abbexa | 100 µl | EUR 737.5 |
T1 Cloning Kit |
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| abx098055-1ml | Abbexa | 1 ml | Ask for price |
T1 Cloning Kit |
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| abx098055-200l | Abbexa | 200 µl | EUR 950 |
T1 Cloning Kit |
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| 20-abx098055 | Abbexa |
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T3 Cloning Kit |
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| abx098057-100l | Abbexa | 100 µl | EUR 737.5 |
T3 Cloning Kit |
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| abx098057-1ml | Abbexa | 1 ml | Ask for price |
T3 Cloning Kit |
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| abx098057-200l | Abbexa | 200 µl | EUR 950 |
T3 Cloning Kit |
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| 20-abx098057 | Abbexa |
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PCR Cloning＜1kb |
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| GC0018 | GenCefe Co Ltd | ＜1kb | EUR 59 |
Blunt Cloning Kit |
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| abx098059-100l | Abbexa | 100 µl | EUR 737.5 |
Blunt Cloning Kit |
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| abx098059-1ml | Abbexa | 1 ml | Ask for price |
Blunt Cloning Kit |
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| abx098059-200l | Abbexa | 200 µl | EUR 950 |
Blunt Cloning Kit |
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| 20-abx098059 | Abbexa |
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Blunt3 Cloning Kit |
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| abx098061-100l | Abbexa | 100 µl | EUR 737.5 |
Blunt3 Cloning Kit |
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| abx098061-1ml | Abbexa | 1 ml | Ask for price |
Blunt3 Cloning Kit |
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| abx098061-200l | Abbexa | 200 µl | EUR 950 |
Blunt3 Cloning Kit |
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| 20-abx098061 | Abbexa |
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F2 cloning plasmid |
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| CSB-CL007923HU-10ug | Cusabio | 10ug | EUR 759.6 |
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Description: A cloning plasmid for the F2 gene. |
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F3 cloning plasmid |
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| CSB-CL007928HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the F3 gene. |
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F7 cloning plasmid |
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| CSB-CL007930HU-10ug | Cusabio | 10ug | EUR 579.6 |
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Description: A cloning plasmid for the F7 gene. |
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F8 cloning plasmid |
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| CSB-CL007932HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the F8 gene. |
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F9 cloning plasmid |
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| CSB-CL007936HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the F9 gene. |
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FH cloning plasmid |
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| CSB-CL008659HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the FH gene. |
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GC cloning plasmid |
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| CSB-CL009306HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GC gene. |
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HP cloning plasmid |
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| CSB-CL010691HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the HP gene. |
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HP cloning plasmid |
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| CSB-CL010691HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the HP gene. |
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PC cloning plasmid |
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| CSB-CL017511HU-10ug | Cusabio | 10ug | EUR 1502.4 |
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Description: A cloning plasmid for the PC gene. |
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MB cloning plasmid |
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| CSB-CL013529HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the MB gene. |
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MB cloning plasmid |
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| CSB-CL013529HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the MB gene. |
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C5 cloning plasmid |
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| CSB-CL003995HU1-10ug | Cusabio | 10ug | EUR 3324 |
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Description: A cloning plasmid for the C5 gene. |
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C5 cloning plasmid |
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| CSB-CL003995HU2-10ug | Cusabio | 10ug | EUR 3324 |
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Description: A cloning plasmid for the C5 gene. |
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C6 cloning plasmid |
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| CSB-CL004036HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the C6 gene. |
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C7 cloning plasmid |
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| CSB-CL004145HU-10ug | Cusabio | 10ug | EUR 669.6 |
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Description: A cloning plasmid for the C7 gene. |
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C9 cloning plasmid |
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| CSB-CL004259HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the C9 gene. |
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CS cloning plasmid |
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| CSB-CL006031HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CS gene. |
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CS cloning plasmid |
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| CSB-CL006031HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CS gene. |
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XG cloning plasmid |
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| CSB-CL026190HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the XG gene. |
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XK cloning plasmid |
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| CSB-CL026198HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the XK gene. |
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GK cloning plasmid |
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| CSB-CL300097HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GK gene. |
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IK cloning plasmid |
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| CSB-CL613381HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the IK gene. |
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SHE cloning plasmid |
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| CSB-CL730142HU-10ug | Cusabio | 10ug | EUR 632.4 |
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Description: A cloning plasmid for the SHE gene. |
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GK5 cloning plasmid |
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| CSB-CL744414HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GK5 gene. |
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EMB cloning plasmid |
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| CSB-CL754239HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the EMB gene. |
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IYD cloning plasmid |
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| CSB-CL754272HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the IYD gene. |
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KLB cloning plasmid |
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| CSB-CL769815HU-10ug | Cusabio | 10ug | EUR 1346.4 |
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Description: A cloning plasmid for the KLB gene. |
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PXK cloning plasmid |
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| CSB-CL770363HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the PXK gene. |
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RD3 cloning plasmid |
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| CSB-CL800101HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the RD3 gene. |
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OAF cloning plasmid |
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| CSB-CL803119HU-10ug | Cusabio | 10ug | EUR 406.8 |
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Description: A cloning plasmid for the OAF gene. |
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NRM cloning plasmid |
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| CSB-CL816902HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the NRM gene. |
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MKX cloning plasmid |
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| CSB-CL818257HU-10ug | Cusabio | 10ug | EUR 487.2 |
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Description: A cloning plasmid for the MKX gene. |
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AEN cloning plasmid |
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| CSB-CL819883HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the AEN gene. |
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AEN cloning plasmid |
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| CSB-CL819883HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the AEN gene. |
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AEN cloning plasmid |
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| CSB-CL819883HU3-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the AEN gene. |
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DAP cloning plasmid |
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| CSB-CL006496HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the DAP gene. |
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DBH cloning plasmid |
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| CSB-CL006518HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the DBH gene. |
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DBI cloning plasmid |
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| CSB-CL006519HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the DBI gene. |
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DCD cloning plasmid |
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| CSB-CL006537HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the DCD gene. |
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DCK cloning plasmid |
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| CSB-CL006547HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the DCK gene. |
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DCN cloning plasmid |
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| CSB-CL006554HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the DCN gene. |
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DCT cloning plasmid |
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| CSB-CL006562HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the DCT gene. |
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DCX cloning plasmid |
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| CSB-CL006576HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the DCX gene. |
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DDC cloning plasmid |
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| CSB-CL006583HU-10ug | Cusabio | 10ug | EUR 616.8 |
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Description: A cloning plasmid for the DDC gene. |
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DDO cloning plasmid |
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| CSB-CL006593HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the DDO gene. |
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DEK cloning plasmid |
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| CSB-CL006710HU-10ug | Cusabio | 10ug | EUR 511.2 |
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Description: A cloning plasmid for the DEK gene. |
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DES cloning plasmid |
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| CSB-CL006735HU-10ug | Cusabio | 10ug | EUR 607.2 |
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Description: A cloning plasmid for the DES gene. |
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DMD cloning plasmid |
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| CSB-CL006963HU-10ug | Cusabio | 10ug | EUR 772.8 |
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Description: A cloning plasmid for the DMD gene. |
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DR1 cloning plasmid |
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| CSB-CL007175HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the DR1 gene. |
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DYM cloning plasmid |
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| CSB-CL007291HU-10ug | Cusabio | 10ug | EUR 568.8 |
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Description: A cloning plasmid for the DYM gene. |
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ECD cloning plasmid |
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| CSB-CL007370HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the ECD gene. |
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EED cloning plasmid |
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| CSB-CL007408HU-10ug | Cusabio | 10ug | EUR 602.4 |
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Description: A cloning plasmid for the EED gene. |
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EFS cloning plasmid |
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| CSB-CL007470HU-10ug | Cusabio | 10ug | EUR 603.6 |
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Description: A cloning plasmid for the EFS gene. |
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EGF cloning plasmid |
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| CSB-CL007473HU-10ug | Cusabio | 10ug | EUR 1536 |
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Description: A cloning plasmid for the EGF gene. |
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EHF cloning plasmid |
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| CSB-CL007494HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the EHF gene. |
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ELL cloning plasmid |
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| CSB-CL007608HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the ELL gene. |
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ELN cloning plasmid |
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| CSB-CL007617HU-10ug | Cusabio | 10ug | EUR 795.6 |
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Description: A cloning plasmid for the ELN gene. |
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EMD cloning plasmid |
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| CSB-CL007633HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the EMD gene. |
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ENG cloning plasmid |
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| CSB-CL007667HU-10ug | Cusabio | 10ug | EUR 795.6 |
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Description: A cloning plasmid for the ENG gene. |
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ERF cloning plasmid |
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| CSB-CL007780HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the ERF gene. |
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ERG cloning plasmid |
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| CSB-CL007781HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the ERG gene. |
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ERH cloning plasmid |
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| CSB-CL007785HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the ERH gene. |
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ESD cloning plasmid |
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| CSB-CL007823HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the ESD gene. |
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EZR cloning plasmid |
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| CSB-CL007914HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the EZR gene. |
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F10 cloning plasmid |
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| CSB-CL007915HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the F10 gene. |
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F11 cloning plasmid |
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| CSB-CL007916HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the F11 gene. |
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F12 cloning plasmid |
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| CSB-CL007918HU-10ug | Cusabio | 10ug | EUR 435.6 |
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Description: A cloning plasmid for the F12 gene. |
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F2R cloning plasmid |
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| CSB-CL007924HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the F2R gene. |
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FAH cloning plasmid |
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| CSB-CL007965HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the FAH gene. |
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FAS cloning plasmid |
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| CSB-CL008433HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the FAS gene. |
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FES cloning plasmid |
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| CSB-CL008597HU-10ug | Cusabio | 10ug | EUR 568.8 |
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Description: A cloning plasmid for the FES gene. |
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FGA cloning plasmid |
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| CSB-CL008607HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the FGA gene. |
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FGA cloning plasmid |
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| CSB-CL008607HU2-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the FGA gene. |
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FGB cloning plasmid |
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| CSB-CL008608HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the FGB gene. |
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FGG cloning plasmid |
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| CSB-CL008651HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the FGG gene. |
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FN1 cloning plasmid |
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| CSB-CL008759HU-10ug | Cusabio | 10ug | EUR 3324 |
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Description: A cloning plasmid for the FN1 gene. |
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FOS cloning plasmid |
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| CSB-CL008790HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the FOS gene. |
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FRK cloning plasmid |
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| CSB-CL008988HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the FRK gene. |
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FTL cloning plasmid |
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| CSB-CL009049HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the FTL gene. |
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FTL cloning plasmid |
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| CSB-CL009049HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the FTL gene. |
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FTL cloning plasmid |
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| CSB-CL009049HU3-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the FTL gene. |
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FTL cloning plasmid |
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| CSB-CL009049HU4-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the FTL gene. |
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FTL cloning plasmid |
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| CSB-CL009049HU5-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the FTL gene. |
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FTL cloning plasmid |
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| CSB-CL009049HU6-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the FTL gene. |
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FUS cloning plasmid |
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| CSB-CL009069HU-10ug | Cusabio | 10ug | EUR 662.4 |
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Description: A cloning plasmid for the FUS gene. |
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FYB cloning plasmid |
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| CSB-CL009099HU-10ug | Cusabio | 10ug | EUR 968.4 |
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Description: A cloning plasmid for the FYB gene. |
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FYN cloning plasmid |
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| CSB-CL009101HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the FYN gene. |
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GAK cloning plasmid |
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| CSB-CL009190HU-10ug | Cusabio | 10ug | EUR 552 |
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Description: A cloning plasmid for the GAK gene. |
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GAL cloning plasmid |
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| CSB-CL009191HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GAL gene. |
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GAN cloning plasmid |
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| CSB-CL009228HU-10ug | Cusabio | 10ug | EUR 734.4 |
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Description: A cloning plasmid for the GAN gene. |
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GCA cloning plasmid |
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| CSB-CL009307HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GCA gene. |
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GCG cloning plasmid |
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| CSB-CL009315HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GCG gene. |
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GCK cloning plasmid |
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| CSB-CL009319HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GCK gene. |
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GDA cloning plasmid |
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| CSB-CL009337HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GDA gene. |
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GEM cloning plasmid |
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| CSB-CL009362HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GEM gene. |
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GGN cloning plasmid |
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| CSB-CL009390HU-10ug | Cusabio | 10ug | EUR 789.6 |
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Description: A cloning plasmid for the GGN gene. |
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GH2 cloning plasmid |
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| CSB-CL009408HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GH2 gene. |
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GIF cloning plasmid |
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| CSB-CL009417HU-10ug | Cusabio | 10ug | EUR 553.2 |
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Description: A cloning plasmid for the GIF gene. |
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GIP cloning plasmid |
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| CSB-CL009434HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GIP gene. |
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GIP cloning plasmid |
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| CSB-CL009434HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GIP gene. |
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GK2 cloning plasmid |
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| CSB-CL009466HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the GK2 gene. |
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GLA cloning plasmid |
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| CSB-CL009474HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GLA gene. |
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GNS cloning plasmid |
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| CSB-CL009639HU-10ug | Cusabio | 10ug | EUR 688.8 |
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Description: A cloning plasmid for the GNS gene. |
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GP2 cloning plasmid |
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| CSB-CL009687HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GP2 gene. |
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GP9 cloning plasmid |
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| CSB-CL009690HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GP9 gene. |
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GRN cloning plasmid |
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| CSB-CL009939HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GRN gene. |
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GRN cloning plasmid |
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| CSB-CL009939HU2-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the GRN gene. |
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GRP cloning plasmid |
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| CSB-CL009940HU-10ug | Cusabio | 10ug | EUR 280.8 |
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Description: A cloning plasmid for the GRP gene. |
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On this setup, the goal gene is tagged with an artificial motif dictating protein degradation. When the system options had been characterised utilizing the monomeric superfolder GFP as a mannequin protein, the basal ranges of fluorescence had been introduced right down to zero (i.e. under the restrict of detection). In all, these novel expression techniques represent another device to altogether suppress leaky gene expression, and they are often simply tailored to different vector codecs and plugged-in into totally different Gram-negative bacterial species on the person’s will.