Background
Vectors based mostly on plant viruses are essential instruments for purposeful genomics, mobile biology, plant genome engineering and molecular farming. We beforehand reported on the development of PepGFPvector based mostly on pepino mosaic virus (PepMV) which expressed GFP effectively and stably in vegetation of its experimental host Nicotiana benthamiana, however not in its pure host tomato. We have now ready a brand new set of PepMV-based vectors with improved stability which can be in a position to specific a variety of reporter genes, helpful for each N. benthamiana and tomato.
Outcomes
We first examined PepGFPm1 and PepGFPm2, two variants of PepGFPvectors had improved GFP expression ranges and stability in N. benthamiana however not in tomato vegetation. Subsequent, we changed GFP by DsRed or mCherry within the new vectors PepDsRed and PepmCherry, respectively; whereas PepmCherry behaved equally to PepGFPm2, PepDsRed expressed the reporter gene effectively additionally in tomato vegetation. We then used PepGFPm2 and PepDsRed to review the PepMV localization in each N. benthamiana and tomato cells. Utilizing confocal laser scanning microscopy (CLSM), we noticed attribute fluorescent our bodies in PepMV-infected cells; these our bodies had a cytoplasmic localization and appeared in shut proximity to the cell nucleus. Already at Three days post-agroinoculation there have been fluorescent our bodies in virtually each cell of agroinoculated tissues of each hosts, and all the time one physique per cell.
When markers for the endoplasmic reticulum or the Golgi equipment had been co-expressed with PepGFPm2 or PepDsRed, a reorganisation of those organelles was noticed, with photos suggesting that each are intimately associated however not the principle constituents of the PepMV our bodies. Altogether, this set of information steered that the PepMV our bodies are much like the potato virus X (PVX) “X-bodies”, which have been described because the PVX viral replication complexes (VRCs). To finish the set of PepMV-based vectors, we constructed a vector expressing the BAR herbicide resistance gene, helpful for large susceptibility screenings.
Conclusions
We have now considerably expanded the PepMV device field by producing a set of recent vectors with improved stability and effectivity in each N. benthamiana and tomato vegetation. By utilizing two of those vectors, we now have described attribute mobile our bodies induced by PepMV an infection; these our bodies are doubtless the PepMV VRCs.
A Artificial Plasmid Toolkit for Shewanella oneidensis MR-1.
Shewanella oneidensis MR-1 is a platform microorganism for understanding extracellular electron switch (EET) with a completely sequenced and annotated genome. Compared to different mannequin microorganisms equivalent to Escherichia coli, the obtainable plasmid elements (equivalent to promoters and replicons) are usually not adequate to conveniently and rapidly fine-tune the expression of a number of genes in S. oneidensis MR-1. Right here, we constructed and characterised a plasmid toolkit that comprises a set of expression vectors with a mixture of promoters, replicons, antibiotic resistance genes, and an RK2 origin of switch (oriT) cassette, during which every ingredient may be simply modified by mounted restriction enzyme websites. The expression cassette can also be suitable with BioBrick artificial biology requirements. Utilizing inexperienced fluorescent protein (GFP) as a reporter, we examined and quantified the energy of promoters.
The copy variety of totally different replicons was additionally measured by real-time quantitative PCR. We additional remodeled two suitable plasmids with totally different antibiotic resistance genes into the recombinant S. oneidensis MR-1, enabling management over the expression of two totally different fluorescent proteins. This plasmid toolkit was additional used for overexpression of the MtrCAB porin-c-type cytochrome complicated within the S. oneidensis ΔmtrA pressure. Tungsten trioxide (WO3) discount and microbial gasoline cell (MFC) assays revealed that the EET effectivity was improved most importantly when MtrCAB was expressed at a average stage, thus demonstrating the utility of the plasmid toolkit within the EET regulation in S. oneidensis. The plasmid toolkit developed on this examine is helpful for fast and handy fine-tuning of gene expression and enhances the power to genetically manipulate S. oneidensis MR-1.
Second generation of pepino mosaic virus vectors: improved stability in tomato and a wide range of reporter genes.
Bodily decoupling of XylS/Pm regulatory parts and conditional proteolysis allow exact management of gene expression in Pseudomonas putida.
Many of the gene expression techniques obtainable for Gram-negative micro organism are stricken by comparatively excessive ranges of basal (i.e. leaky) expression of the goal gene(s). This prevalence impacts the system dynamics, finally lowering the output and productiveness of engineered pathways and artificial circuits.
With the intention to circumvent this downside, we now have designed a novel expression system based mostly on the well-known XylS/Pm transcriptional regulator/promoter pair from the soil bacterium Pseudomonas putida mt-2, during which the important thing purposeful parts are bodily decoupled. By integrating the xylS gene into the chromosome of the platform pressure KT2440, whereas putting the Pm promoter right into a set of ordinary plasmid vectors, the inducibility of the system (i.e. the output distinction between the induced and uninduced state) improved as much as 170-fold. We additional mixed this modular system with an additional layer of post-translational management by the use of conditional proteolysis.
Cloning Optimizer |
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| MBS4156417-5x02mL | MyBiosource | 5x0.2mL | EUR 795 |
T1 Cloning Kit |
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| 20-abx098055 | Abbexa |
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T3 Cloning Kit |
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| 20-abx098057 | Abbexa |
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T1 Cloning Kit |
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| abx098055-100l | Abbexa | 100 µl | EUR 737.5 |
T1 Cloning Kit |
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| abx098055-1ml | Abbexa | 1 ml | Ask for price |
T1 Cloning Kit |
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| abx098055-200l | Abbexa | 200 µl | EUR 950 |
T3 Cloning Kit |
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| abx098057-100l | Abbexa | 100 µl | EUR 737.5 |
T3 Cloning Kit |
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| abx098057-1ml | Abbexa | 1 ml | Ask for price |
T3 Cloning Kit |
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| abx098057-200l | Abbexa | 200 µl | EUR 950 |
Blunt Cloning Kit |
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| 20-abx098059 | Abbexa |
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Blunt Cloning Kit |
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| abx098059-100l | Abbexa | 100 µl | EUR 737.5 |
Blunt Cloning Kit |
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| abx098059-1ml | Abbexa | 1 ml | Ask for price |
Blunt Cloning Kit |
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| abx098059-200l | Abbexa | 200 µl | EUR 950 |
Blunt3 Cloning Kit |
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| 20-abx098061 | Abbexa |
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C5 cloning plasmid |
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| CSB-CL003995HU1-10ug | Cusabio | 10ug | EUR 3324 |
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Description: A cloning plasmid for the C5 gene. |
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C5 cloning plasmid |
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| CSB-CL003995HU2-10ug | Cusabio | 10ug | EUR 3324 |
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Description: A cloning plasmid for the C5 gene. |
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C6 cloning plasmid |
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| CSB-CL004036HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the C6 gene. |
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C7 cloning plasmid |
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| CSB-CL004145HU-10ug | Cusabio | 10ug | EUR 669.6 |
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Description: A cloning plasmid for the C7 gene. |
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C9 cloning plasmid |
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| CSB-CL004259HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the C9 gene. |
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CS cloning plasmid |
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| CSB-CL006031HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CS gene. |
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CS cloning plasmid |
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| CSB-CL006031HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CS gene. |
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F2 cloning plasmid |
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| CSB-CL007923HU-10ug | Cusabio | 10ug | EUR 759.6 |
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Description: A cloning plasmid for the F2 gene. |
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F3 cloning plasmid |
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| CSB-CL007928HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the F3 gene. |
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F7 cloning plasmid |
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| CSB-CL007930HU-10ug | Cusabio | 10ug | EUR 579.6 |
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Description: A cloning plasmid for the F7 gene. |
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F8 cloning plasmid |
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| CSB-CL007932HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the F8 gene. |
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F9 cloning plasmid |
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| CSB-CL007936HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the F9 gene. |
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FH cloning plasmid |
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| CSB-CL008659HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the FH gene. |
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GC cloning plasmid |
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| CSB-CL009306HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GC gene. |
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HP cloning plasmid |
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| CSB-CL010691HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the HP gene. |
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HP cloning plasmid |
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| CSB-CL010691HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the HP gene. |
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MB cloning plasmid |
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| CSB-CL013529HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the MB gene. |
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MB cloning plasmid |
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| CSB-CL013529HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the MB gene. |
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PC cloning plasmid |
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| CSB-CL017511HU-10ug | Cusabio | 10ug | EUR 1502.4 |
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Description: A cloning plasmid for the PC gene. |
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IK cloning plasmid |
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| CSB-CL613381HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the IK gene. |
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XG cloning plasmid |
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| CSB-CL026190HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the XG gene. |
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XK cloning plasmid |
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| CSB-CL026198HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the XK gene. |
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GK cloning plasmid |
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| CSB-CL300097HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the GK gene. |
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Blunt3 Cloning Kit |
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| abx098061-100l | Abbexa | 100 µl | EUR 737.5 |
Blunt3 Cloning Kit |
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| abx098061-1ml | Abbexa | 1 ml | Ask for price |
Blunt3 Cloning Kit |
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| abx098061-200l | Abbexa | 200 µl | EUR 950 |
FUZ cloning plasmid |
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| CSB-CL883593HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the FUZ gene. |
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FUZ cloning plasmid |
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| CSB-CL883593HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the FUZ gene. |
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LY9 cloning plasmid |
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| CSB-CL884511HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the LY9 gene. |
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LY9 cloning plasmid |
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| CSB-CL884511HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the LY9 gene. |
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LY9 cloning plasmid |
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| CSB-CL884511HU3-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the LY9 gene. |
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EVL cloning plasmid |
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| CSB-CL887052HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the EVL gene. |
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EVL cloning plasmid |
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| CSB-CL887052HU2-10ug | Cusabio | 10ug | EUR 552 |
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Description: A cloning plasmid for the EVL gene. |
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DTL cloning plasmid |
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| CSB-CL889160HU1-10ug | Cusabio | 10ug | EUR 568.8 |
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Description: A cloning plasmid for the DTL gene. |
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DTL cloning plasmid |
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| CSB-CL889160HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the DTL gene. |
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NLK cloning plasmid |
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| CSB-CL890657HU-10ug | Cusabio | 10ug | EUR 652.8 |
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Description: A cloning plasmid for the NLK gene. |
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TES cloning plasmid |
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| CSB-CL890679HU-10ug | Cusabio | 10ug | EUR 556.8 |
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Description: A cloning plasmid for the TES gene. |
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HN1 cloning plasmid |
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| CSB-CL890732HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the HN1 gene. |
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DSE cloning plasmid |
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| CSB-CL890925HU-10ug | Cusabio | 10ug | EUR 669.6 |
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Description: A cloning plasmid for the DSE gene. |
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ICK cloning plasmid |
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| CSB-CL891800HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the ICK gene. |
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GNE cloning plasmid |
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| CSB-CL897458HU-10ug | Cusabio | 10ug | EUR 860.4 |
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Description: A cloning plasmid for the GNE gene. |
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UXT cloning plasmid |
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| CSB-CL880062HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the UXT gene. |
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FTO cloning plasmid |
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| CSB-CL880154HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the FTO gene. |
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FTO cloning plasmid |
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| CSB-CL880154HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the FTO gene. |
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AK3 cloning plasmid |
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| CSB-CL883438HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the AK3 gene. |
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EMB cloning plasmid |
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| CSB-CL754239HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the EMB gene. |
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IYD cloning plasmid |
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| CSB-CL754272HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the IYD gene. |
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CD6 cloning plasmid |
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| CSB-CL326953HU-10ug | Cusabio | 10ug | EUR 805.2 |
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Description: A cloning plasmid for the CD6 gene. |
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AXL cloning plasmid |
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| CSB-CL326981HU-10ug | Cusabio | 10ug | EUR 1033.2 |
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Description: A cloning plasmid for the AXL gene. |
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ADK cloning plasmid |
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| CSB-CL346232HU-10ug | Cusabio | 10ug | EUR 481.2 |
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Description: A cloning plasmid for the ADK gene. |
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ABO cloning plasmid |
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| CSB-CL001110HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the ABO gene. |
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ABO cloning plasmid |
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| CSB-CL001110HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the ABO gene. |
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ADA cloning plasmid |
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| CSB-CL001268HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the ADA gene. |
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ADC cloning plasmid |
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| CSB-CL001330HU-10ug | Cusabio | 10ug | EUR 596.4 |
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Description: A cloning plasmid for the ADC gene. |
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AFM cloning plasmid |
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| CSB-CL001419HU-10ug | Cusabio | 10ug | EUR 736.8 |
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Description: A cloning plasmid for the AFM gene. |
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AFP cloning plasmid |
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| CSB-CL001421HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the AFP gene. |
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AGA cloning plasmid |
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| CSB-CL001423HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the AGA gene. |
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AGT cloning plasmid |
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| CSB-CL001463HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the AGT gene. |
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AHR cloning plasmid |
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| CSB-CL001481HU-10ug | Cusabio | 10ug | EUR 986.4 |
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Description: A cloning plasmid for the AHR gene. |
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AK1 cloning plasmid |
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| CSB-CL001508HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the AK1 gene. |
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AK2 cloning plasmid |
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| CSB-CL001509HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the AK2 gene. |
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AK7 cloning plasmid |
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| CSB-CL001517HU-10ug | Cusabio | 10ug | EUR 793.2 |
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Description: A cloning plasmid for the AK7 gene. |
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ALB cloning plasmid |
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| CSB-CL001561HU1-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the ALB gene. |
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ALB cloning plasmid |
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| CSB-CL001561HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the ALB gene. |
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AMT cloning plasmid |
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| CSB-CL001684HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the AMT gene. |
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ANG cloning plasmid |
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| CSB-CL001703HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the ANG gene. |
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ANG cloning plasmid |
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| CSB-CL001703HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the ANG gene. |
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APP cloning plasmid |
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| CSB-CL001950HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the APP gene. |
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ARC cloning plasmid |
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| CSB-CL001981HU-10ug | Cusabio | 10ug | EUR 531.6 |
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Description: A cloning plasmid for the ARC gene. |
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ASL cloning plasmid |
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| CSB-CL002213HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the ASL gene. |
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AVP cloning plasmid |
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| CSB-CL002466HU-10ug | Cusabio | 10ug | EUR 297.6 |
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Description: A cloning plasmid for the AVP gene. |
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B2M cloning plasmid |
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| CSB-CL002486HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the B2M gene. |
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BGN cloning plasmid |
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| CSB-CL002683HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the BGN gene. |
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BID cloning plasmid |
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| CSB-CL002698HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the BID gene. |
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BID cloning plasmid |
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| CSB-CL002698HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the BID gene. |
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BLK cloning plasmid |
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| CSB-CL002714HU-10ug | Cusabio | 10ug | EUR 642 |
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Description: A cloning plasmid for the BLK gene. |
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BMX cloning plasmid |
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| CSB-CL002761HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the BMX gene. |
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BPI cloning plasmid |
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| CSB-CL002783HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the BPI gene. |
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BSG cloning plasmid |
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| CSB-CL002831HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the BSG gene. |
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BTC cloning plasmid |
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| CSB-CL002853HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the BTC gene. |
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BTD cloning plasmid |
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| CSB-CL002854HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the BTD gene. |
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C1R cloning plasmid |
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| CSB-CL003655HU-10ug | Cusabio | 10ug | EUR 568.8 |
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Description: A cloning plasmid for the C1R gene. |
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C8A cloning plasmid |
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| CSB-CL004194HU1-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the C8A gene. |
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C8A cloning plasmid |
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| CSB-CL004194HU2-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the C8A gene. |
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C8B cloning plasmid |
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| CSB-CL004195HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the C8B gene. |
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C8G cloning plasmid |
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| CSB-CL004196HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the C8G gene. |
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CA1 cloning plasmid |
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| CSB-CL004364HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CA1 gene. |
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CA2 cloning plasmid |
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| CSB-CL004370HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CA2 gene. |
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CA3 cloning plasmid |
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| CSB-CL004371HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CA3 gene. |
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CA4 cloning plasmid |
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| CSB-CL004372HU-10ug | Cusabio | 10ug | EUR 447.6 |
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Description: A cloning plasmid for the CA4 gene. |
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CA6 cloning plasmid |
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| CSB-CL004377HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CA6 gene. |
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CA7 cloning plasmid |
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| CSB-CL004378HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CA7 gene. |
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CA8 cloning plasmid |
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| CSB-CL004379HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CA8 gene. |
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CA8 cloning plasmid |
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| CSB-CL004379HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CA8 gene. |
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CA8 cloning plasmid |
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| CSB-CL004379HU3-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CA8 gene. |
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CAT cloning plasmid |
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| CSB-CL004563HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the CAT gene. |
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CBS cloning plasmid |
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| CSB-CL004589HU1-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the CBS gene. |
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CBS cloning plasmid |
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| CSB-CL004589HU2-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the CBS gene. |
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CBS cloning plasmid |
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| CSB-CL004589HU3-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the CBS gene. |
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CCK cloning plasmid |
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| CSB-CL004771HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CCK gene. |
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CD2 cloning plasmid |
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| CSB-CL004894HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CD2 gene. |
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CD4 cloning plasmid |
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| CSB-CL004935HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CD4 gene. |
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CD7 cloning plasmid |
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| CSB-CL004953HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CD7 gene. |
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CD9 cloning plasmid |
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| CSB-CL004969HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CD9 gene. |
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CDA cloning plasmid |
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| CSB-CL004976HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CDA gene. |
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CFB cloning plasmid |
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| CSB-CL005268HU-10ug | Cusabio | 10ug | EUR 568.8 |
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Description: A cloning plasmid for the CFB gene. |
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CFD cloning plasmid |
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| CSB-CL005271HU-10ug | Cusabio | 10ug | EUR 386.4 |
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Description: A cloning plasmid for the CFD gene. |
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CFH cloning plasmid |
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| CSB-CL005273HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CFH gene. |
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CFI cloning plasmid |
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| CSB-CL005279HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CFI gene. |
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CFP cloning plasmid |
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| CSB-CL005291HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CFP gene. |
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CGA cloning plasmid |
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| CSB-CL005293HU1-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CGA gene. |
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CGA cloning plasmid |
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| CSB-CL005293HU2-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CGA gene. |
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CGB cloning plasmid |
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| CSB-CL005294HU-10ug | Cusabio | 10ug | EUR 279.6 |
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Description: A cloning plasmid for the CGB gene. |
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On this setup, the goal gene is tagged with an artificial motif dictating protein degradation. When the system options had been characterised utilizing the monomeric superfolder GFP as a mannequin protein, the basal ranges of fluorescence had been introduced right down to zero (i.e. under the restrict of detection). In all, these novel expression techniques represent another device to altogether suppress leaky gene expression, and they are often simply tailored to different vector codecs and plugged-in into totally different Gram-negative bacterial species on the person’s will.