Triglycerides (TG) are the main component of body fat in humans and animals, as well as vegetable fat. TGs are found as a natural component in the human blood circulatory system. TG is made up of one glycerol molecule and 3 fatty acid molecules. Cholesterol metabolism generates fatty acids and glycerol, both of which can serve as substrates in metabolic pathways to produce energy.
Excess TG can remain in the body in the form of fat deposits and that can lead to obesity. Elevated triglyceride levels also increase the risk of heart disease, stroke, and type 2 diabetes. BioVision’s EZScreen Triglyceride Quantitation Kit provides an easy and sensitive assay for measuring TG concentration in a variety of samples. In the test, TGs are converted to free fatty acids and glycerol.
The released glycerol is then oxidized to generate a product that reacts with a probe to generate a color that can be detected by a spectrophotometer at λ = 590 nm. The method is quantitative, fast, simple, sensitive, and designed for high-performance formats. The kit provides a convenient means of detecting 0.5 to 5 mM triglycerides in biological samples.
Abnormal high blood lactate concentration has been linked to many major diseases such as diabetes and lactate acidosis. Elevated blood lactate concentration is also associated with shock, post-cardiac arrest, tissue ischemia, thiamine deficiency, liver disorders, mitochondrial diseases, and many more. Therefore, the detection and quantification of lactate in blood is a diagnostic key for several diseases. The BioVision EZScreen Lactate Colorimetric Assay Kit uses a mixture of enzymes that specifically reacts with L-lactate.
D-lactate does not react with the enzyme mixture. The enzymatic reaction with L-lactate produces a product that generates a color (OD = 590 nm) when it reacts with the lactate probe. The product formed is directly proportional to the amount of L-lactate present in the sample. The method is quantitative, fast, simple, sensitive, and designed for high-performance formats. The kit provides a convenient means of detecting 0.5 to 5 mM lactate in biological samples such as blood, bloodstream, cells, culture media, fermentation media, etc. in 384 HTP format