Adeno-associated virus (AAV) vectors are at present among the many mostly utilized for in vivo gene remedy approaches. The analysis of vectors throughout medical improvement requires the manufacturing of appreciable quantities of extremely pure and potent vectors. Right here, we set up a scalable course of for AAV manufacturing, utilizing orbitally shaken bioreactors and a completely characterised suspension-adapted cell line, HEKExpress. We carried out a proof-of-concept manufacturing of AAV2/eight and AAV2/9 vectors utilizing HEKExpress cells.
Moreover, we in contrast the manufacturing of AAV2/9 vectors utilizing this suspension cell line to classical protocols based mostly on adherent HEK293 cells to show bioequivalence in vitro and in vivo. Following upstream processing, we purified vectors by way of gradient centrifugation and immunoaffinity chromatography. The in vitro characterization revealed variations because of the purification methodology, in addition to the transfection protocol and the corresponding HEK293 cell line. The purification methodology and cell line used additionally affected in vivo transduction effectivity after bilateral injection of AAV2/9 vectors expressing a GFP reporter fused with a nuclear localization sign (AAV2/9-CBA-nlsGFP) into the striatum of grownup mice.
These outcomes present that AAV vectors deriving from suspension HEKExpress cells are bioequivalent and should exhibit larger efficiency than vectors produced with adherent HEK293 cells.
Lengthy-Vary, Border-Crossing, Horizontal Axon Radiations Are a Widespread Function of Rat Neocortical Areas That Differ in Cytoarchitecture.
Simplified inducible system for Trypanosoma brucei.
T&A Cloning Vector |
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FYC002-20P | Yeastern Biotech | 1 vial | Ask for price |
pTG19-T PCR Cloning Vector, 20apps |
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TA010 | Vivantis | each | Ask for price |
pET His6 Sumo TEV LIC cloning vector (2S-T) |
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PVT14035 | Nova Lifetech | 2ug | EUR 480 |
siRNA Cloning Vector (pGB) |
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9500-20 | Biovision | each | EUR 352.8 |
dCas9 C-terminal Cloning Vector |
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K014 | ABM | 10 ug | EUR 545 |
Description: N/A |
pcDNA3 GFP LIC cloning vector (6D) |
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PVT10748 | Nova Lifetech | 2ug | EUR 215 |
pLenti-III-mir-GFP Cloning Vector |
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m016 | ABM | 500 ng | EUR 725 |
pET His6 MBP TEV LIC cloning vector (1M) |
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PVT10596 | Nova Lifetech | 2ug | EUR 182 |
pET His6 Sumo TEV LIC cloning vector (1S) |
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PVT14034 | Nova Lifetech | 2ug | EUR 480 |
pFastBac Dual His6 TEV LIC cloning vector(5B) Plasmid |
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PVT43314 | Nova Lifetech | 2ug | EUR 280 |
pFastBac His6 TEV cloning vector with BioBrick polycistronic |
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PVT29881 | Nova Lifetech | 2ug | EUR 280 |
pCDF1-MCS1 cDNA Cloning and Expression Vector |
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CD100A-1 | SBI | 10 ug | EUR 483 |
T&A Cloning Kits |
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FYC001-20P | Yeastern Biotech | 1 vial | Ask for price |
pCDH-CMV-MCS cDNA Cloning and Expression Vector |
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CD500B-1 | SBI | 10 ug | EUR 483 |
pCDH-EF1-MCS cDNA Cloning and Expression Vector |
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CD502A-1 | SBI | 10 ug | EUR 483 |
pCDH-CMV-MCS2 cDNA Cloning and Expression Vector |
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CD501A-1 | SBI | 10 ug | EUR 483 |
pSIH1-H1-Puro shRNA Cloning and Expression Vector |
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SI500A-1 | SBI | 10 ug | EUR 507 |
pSIH1-H1-H2Kk shRNA Cloning and Expression Vector |
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SI502A-1 | SBI | 10 ug | EUR 507 |
pAAVK-EF1α -MCS AAVanced Cloning and Expression Vector |
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AAV502A-1 | SBI | 10 ug | EUR 614 |
pSIH1-H1-copGFP shRNA Cloning and Expression Vector |
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SI501A-1 | SBI | 10 ug | EUR 507 |
Cumate-Inducible (CuO) EEV cloning and expression vector |
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EEV610A-1 | SBI | 10 ug | EUR 614 |
Green Kit. Baculovirus GFP vector. |
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K20 | AB Vector LLC | 1 Kit | EUR 695 |
Description: Protein expression |
pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector |
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CD811A-1 | SBI | 10 ug | EUR 579 |
ProGreen. Baculovirus GFP marker vector. |
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A1 | AB Vector LLC | 25 ul | EUR 420 |
Description: Protein expression |
pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector |
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CD810A-1 | SBI | 10 ug | EUR 579 |
Constitutive (CAGs promoter) EEV cloning and expression vector |
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EEV600A-1 | SBI | 10 ug | EUR 614 |
pVL1393. General baculovirus plasmid vector. |
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B1 | AB Vector LLC | 50 ul | EUR 340 |
Description: Protein expression |
pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector |
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CD111B-1 | SBI | 10 ug | EUR 572 |
pCDH-MCS-T2A-copGFP-MSCV Cloning and Expression Vector |
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CD523A-1 | SBI | 10 ug | EUR 590 |
PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector |
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PB511B-1 | SBI | 10 ug | EUR 638 |
PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector |
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PB512B-1 | SBI | 10 ug | EUR 638 |
PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector |
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PB510B-1 | SBI | 10 ug | EUR 638 |
PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector |
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PB530A-2 | SBI | 10 ug | EUR 638 |
PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
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PB531A-2 | SBI | 10 ug | EUR 638 |
PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector |
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PB533A-2 | SBI | 10 ug | EUR 638 |
pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector |
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CD512B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector |
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CD514B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
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CD510B-1 | SBI | 10 ug | EUR 572 |
pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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CD530A-2 | SBI | 10 ug | EUR 590 |
pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
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CD531A-2 | SBI | 10 ug | EUR 590 |
pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector |
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CD533A-2 | SBI | 10 ug | EUR 590 |
pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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CD630A-1 | SBI | 10 ug | EUR 590 |
pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector |
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CD711B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
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CD515B-1 | SBI | 10 ug | EUR 572 |
pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector |
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CD522A-1 | SBI | 10 ug | EUR 590 |
pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector |
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CD532A-2 | SBI | 10 ug | EUR 590 |
pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
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CD615B-1 | SBI | 10 ug | EUR 572 |
pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
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CD710B-1 | SBI | 10 ug | EUR 572 |
pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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CD731B-1 | SBI | 10 ug | EUR 590 |
PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector |
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PB514B-2 | SBI | 10 ug | EUR 638 |
pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector |
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CD511B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector |
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CD524A-1 | SBI | 10 ug | EUR 590 |
ProEasy. Vector for easy construction of recombinant baculoviruses. |
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A10S | AB Vector LLC | 25 ul | EUR 695 |
Description: Protein expression |
PinPoint-HR attP Placement Vector – Empty MCS for cloning HR Arms |
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PIN400A-1 | SBI | 10ug | EUR 1190 |
PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector |
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PBSI505A-1 | SBI | 10 ug | EUR 638 |
PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector |
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PBSI506A-1 | SBI | 10 ug | EUR 638 |
PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector |
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PB513B-1 | SBI | 10 ug | EUR 638 |
pAAVK-EF1α -MCS-T2A-EGFP AAVanced Cloning and Expression Vector |
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AAV526A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α -MCS-T2A-Puro AAVanced Cloning and Expression Vector |
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AAV527A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α –MCS-T2A-mRFP AAVanced Cloning and Expression Vector |
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AAV528A-1 | SBI | 10 ug | EUR 614 |
PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
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PB713B-1 | SBI | 10 ug | EUR 638 |
pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
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CD513B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector |
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CD516B-2 | SBI | 10 ug | EUR 572 |
pAcAB3. Baculovirus plasmid vector for expression of up to 3 proteins. |
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B2 | AB Vector LLC | 50 ul | EUR 420 |
Description: Protein expression |
pAAVK-EF1α-MCS1-CMV-MCS2 AAVanced Cloning and Expression Vector |
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AAV503A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α-MCS1-CMV-EGFP AAVanced Cloning and Expression Vector |
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AAV536A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α-MCS1-CMV-Puro AAVanced Cloning and Expression Vector |
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AAV537A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α-MCS1-CMV-mRFP AAVanced Cloning and Expression Vector |
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AAV538A-1 | SBI | 10 ug | EUR 614 |
pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector |
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CD713B-1 | SBI | 10 ug | EUR 572 |
pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector |
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CD813A-1 | SBI | 10 ug | EUR 579 |
pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector |
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CD822A-1 | SBI | 10 ug | EUR 579 |
pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector |
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CD823A-1 | SBI | 10 ug | EUR 579 |
pAB-bee. Baculovirus plasmid vector for secreted and transmembrane proteins. |
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B3 | AB Vector LLC | 50 ul | EUR 495 |
Description: Protein expression |
T&A Cloning Kit + ECOS 101DH5a |
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FYC005-20P | Yeastern Biotech | 1 vial | Ask for price |
pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 µg |
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CD527A-1 | SBI | 10 ug | EUR 590 |
ProFold-PDI. Baculovirus chaperone vector for expression of cysteine-rich proteins. |
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A7 | AB Vector LLC | 25 ul | EUR 830 |
Description: Protein expression |
gRNA_Cloning Vector |
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PVTY00131 | Nova Lifetech | 2ug | EUR 280 |
ProFold-C1. Baculovirus chaperone vector for expression of cytoplasmic and nuclear proteins. |
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A2 | AB Vector LLC | 25 ul | EUR 830 |
Description: Protein expression |
ProFold-C2. Baculovirus chaperone vector for expression of cytoplasmic and nuclear proteins. |
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A3 | AB Vector LLC | 25 ul | EUR 830 |
Description: Protein expression |
ProFold-ER1. Baculovirus chaperone vector for expression of secreted and membrane proteins. |
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A4 | AB Vector LLC | 25 ul | EUR 830 |
Description: Protein expression |
T&A Cloning Kit + ECOS 9-5JM109 |
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FYC006-20P | Yeastern Biotech | 1 vial | Ask for price |
pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector |
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CD550A-1 | SBI | 10 ug | EUR 563 |
PB-CuO-CMV-MCS-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector |
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PBQM800A-1 | SBI | 10 µg | EUR 921 |
PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector |
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PBQM812A-1 | SBI | 10 ug | EUR 1023 |
Cloning |
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S201 | 101Bio | - | Ask for price |
T-Pro Stacking Buffer |
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JB03-C002 | T-Pro Biotechnology | 500ml/BT | EUR 182.4 |
T-Pro MycoClean spray |
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JT90-R002 | T-Pro Biotechnology | 500ml/BT | EUR 172.8 |
T-Pro Blocking Buffer |
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JK92-W005 | T-Pro Biotechnology | 500ml/BT | EUR 60 |
PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector |
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PBQMSH812A-1 | SBI | 10 ug | EUR 1023 |
C1 Kit. Baculovirus chaperone vectors for cytoplasmic and nuclear proteins. |
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K21 | AB Vector LLC | 1 Kit | EUR 995 |
Description: Protein expression |
C2 Kit. Baculovirus chaperone vectors for cytoplasmic and nuclear proteins. |
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K22 | AB Vector LLC | 1 Kit | EUR 995 |
Description: Protein expression |
ER1 Kit. Baculovirus chaperone vectors for expression of secreted and membrane proteins. |
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K23 | AB Vector LLC | 1 Kit | EUR 995 |
Description: Protein expression |
ER1-bee Kit. Baculovirus chaperone vectors for expression of secreted and membrane proteins. |
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K24 | AB Vector LLC | 1 Kit | EUR 995 |
Description: Protein expression |
T-Pro EZ Gel Solution 8% |
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JB02-B008M | T-Pro Biotechnology | 500ml/BT | EUR 266.4 |
T-Pro EZ Gel Solution 8% |
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JB02-B008S | T-Pro Biotechnology | 100ml/BT | EUR 162 |
T-Pro EZ Gel Solution 10% |
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JB02-B010M | T-Pro Biotechnology | 500ml/BT | EUR 266.4 |
T-Pro EZ Gel Solution 10% |
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JB02-B010S | T-Pro Biotechnology | 100ml/BT | EUR 162 |
T-Pro EZ Gel Solution 12% |
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JB02-B012M | T-Pro Biotechnology | 500ml/BT | EUR 266.4 |
T-Pro EZ Gel Solution 12% |
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JB02-B012S | T-Pro Biotechnology | 100ml/BT | EUR 162 |
T-Pro EZ Gel Solution 15% |
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JB02-B015M | T-Pro Biotechnology | 500ml/BT | EUR 266.4 |
T-Pro EZ Gel Solution 15% |
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JB02-B015S | T-Pro Biotechnology | 100ml/BT | EUR 162 |
T-Pro Separating or Resolving Buffer |
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JB03-C001 | T-Pro Biotechnology | 500ml/BT | EUR 182.4 |
T-Pro BCA Protein Assay kit |
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JB04-D001 | T-Pro Biotechnology | 500assay/KIT | EUR 244.8 |
T-Pro Transfer Blotting buffer (10X) |
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JB08-H001 | T-Pro Biotechnology | 500ml/BT | EUR 162 |
T-Pro Western Blot Stripping Reagent |
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JB11-K002 | T-Pro Biotechnology | 500ml/BT | EUR 213.6 |
T-Pro Aqua EZ Clean (M) |
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JT90-R001M | T-Pro Biotechnology | 500ml/BT | EUR 398.4 |
T-Pro Aqua EZ Clean (S) |
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JT90-R001S | T-Pro Biotechnology | 100ml/BT | EUR 181.2 |
T-Pro EZ stain Gel solution |
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JT90-R005M | T-Pro Biotechnology | 500ml/BT | EUR 224.4 |
T-Pro EZ stain Gel solution |
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JT90-R005S | T-Pro Biotechnology | 100ml/BT | EUR 151.2 |
T-Pro P-Fect Transfection Reagent |
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JT97-N005M | T-Pro Biotechnology | 1.0ml/vial | EUR 266.4 |
T-Pro Plasmid Mini Kit (100) |
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RB94-YPD100 | T-Pro Biotechnology | 100preps/Kit | EUR 193.2 |
T-Pro Plasmid Mini Kit (250) |
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RB94-YPD250 | T-Pro Biotechnology | 250preps/Kit | EUR 266.4 |
T-Pro Plasmid Midi Kit (20) |
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RB94-YPI020 | T-Pro Biotechnology | 20preps/Kit | EUR 255.6 |
T-Pro Plasmid Maxi Kit (10) |
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RB94-YPM010 | T-Pro Biotechnology | 10preps/Kit | EUR 266.4 |
T-Pro EZ Gel Solution 6% |
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JB02-B006M | T-Pro Biotechnology | 500ml/BT | EUR 120 |
T-Pro EZ Gel Solution 6% |
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JB02-B006S | T-Pro Biotechnology | 100ml/BT | EUR 30 |
T-Pro Cell Counting Kit (CCK8) |
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JK95-C008L | T-Pro Biotechnology | 5ml*20/BT | EUR 1000 |
T-Pro Cell Counting Kit (CCK8) |
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JK95-C008M | T-Pro Biotechnology | 5ml*2/BT | EUR 120 |
T-Pro Cell Counting Kit (CCK8) |
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JK95-C008S | T-Pro Biotechnology | 1ml*5/BT | EUR 80 |
T-Pro RIPA Lysis buffer (1X) |
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JT89-L001M | T-Pro Biotechnology | 500ml/BT | EUR 60 |
T-Pro RIPA Lysis buffer (5X) |
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JT89-L005S | T-Pro Biotechnology | 100ml/BT | EUR 60 |
T-Pro Ponceau S stain solution |
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JT90-R006M | T-Pro Biotechnology | 500ml/BT | EUR 60 |
gRNA- Cloning |
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PVT10957 | Nova Lifetech | 2ug | EUR 215 |
T-Pro BCA Protein Assay Reagent A |
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JB04-D001A | T-Pro Biotechnology | 500ml/BT | EUR 204 |
T-Pro BCA Protein Assay Reagent B |
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JB04-D001B | T-Pro Biotechnology | 12ml/BT | EUR 140.4 |
T-Pro Bradford Protein Assay kit(1X) |
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JB04-D002 | T-Pro Biotechnology | 500ml/BT | EUR 193.2 |
T-Pro Fast Blocking Buffer (in PBS) |
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JK92-W001 | T-Pro Biotechnology | 500ml/BT | EUR 224.4 |
T-Pro Fast Blocking Buffer (in TBS) |
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JK92-W002 | T-Pro Biotechnology | 500ml/BT | EUR 224.4 |
T-Pro Genomic DNA Midi Kit (20) |
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RB94-NGM020 | T-Pro Biotechnology | 20preps/kit | EUR 266.4 |
T-Pro Genomic DNA Mini Kit (100) |
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RB94-NGS100 | T-Pro Biotechnology | 100preps/kit | EUR 255.6 |
T-Pro Bradford Protein Assay kit (5X) |
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JB04-D003 | T-Pro Biotechnology | 500ml/BT | EUR 120 |
T-Pro MOPS/SDS running buffer (20X) |
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JB07-G003 | T-Pro Biotechnology | 500ml/BT | EUR 80 |
T-Pro HQ Transfer Blotting buffer (20X) |
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JB08-H003 | T-Pro Biotechnology | 500ml/BT | EUR 80 |
T-Pro Western Blot Stripping Reagent (EX) |
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JB11-K005 | T-Pro Biotechnology | 500ml/BT | EUR 60 |
T-Pro MOPS buffer (1M, pH 6.5) |
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JB12-S001 | T-Pro Biotechnology | 500ml/BT | EUR 60 |
T-Pro MOPS buffer (1M, pH 7.0) |
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JB12-S002 | T-Pro Biotechnology | 500ml/BT | EUR 60 |
T-Pro MOPS buffer (1M, pH 7.5) |
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JB12-S003 | T-Pro Biotechnology | 500ml/BT | EUR 60 |
Cloning Optimizer |
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E004 | ABM | 200 μl | EUR 105 |
Description: Cloning Optimizer consists of a proprietary enzyme that can completely eliminate background colonies when cloning. Treatment of PCR amplified DNA with this innovative optimizer will remove the trace amounts of plasmid template DNA that remain after PCR. Cloning Optimizer eliminates non-linearized template plasmids that cause false-positive colonies. |
Cloning Optimizer |
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MBS4156417-02mL | MyBiosource | 0.2mL | EUR 245 |
Cloning Optimizer |
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MBS4156417-5x02mL | MyBiosource | 5x0.2mL | EUR 795 |
T-Pro Laemmli (SDS sample) Reagent (reducing 4X) |
|||
JB06-F002 | T-Pro Biotechnology | 10ml/BT | EUR 151.2 |