
Adeno-associated virus (AAV) vectors are at present among the many mostly utilized for in vivo gene remedy approaches. The analysis of vectors throughout medical improvement requires the manufacturing of appreciable quantities of extremely pure and potent vectors. Right here, we set up a scalable course of for AAV manufacturing, utilizing orbitally shaken bioreactors and a completely characterised suspension-adapted cell line, HEKExpress. We carried out a proof-of-concept manufacturing of AAV2/eight and AAV2/9 vectors utilizing HEKExpress cells.
Moreover, we in contrast the manufacturing of AAV2/9 vectors utilizing this suspension cell line to classical protocols based mostly on adherent HEK293 cells to show bioequivalence in vitro and in vivo. Following upstream processing, we purified vectors by way of gradient centrifugation and immunoaffinity chromatography. The in vitro characterization revealed variations because of the purification methodology, in addition to the transfection protocol and the corresponding HEK293 cell line. The purification methodology and cell line used additionally affected in vivo transduction effectivity after bilateral injection of AAV2/9 vectors expressing a GFP reporter fused with a nuclear localization sign (AAV2/9-CBA-nlsGFP) into the striatum of grownup mice.
These outcomes present that AAV vectors deriving from suspension HEKExpress cells are bioequivalent and should exhibit larger efficiency than vectors produced with adherent HEK293 cells.
Lengthy-Vary, Border-Crossing, Horizontal Axon Radiations Are a Widespread Function of Rat Neocortical Areas That Differ in Cytoarchitecture.

Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells.
Simplified inducible system for Trypanosoma brucei.
T&A Cloning Vector |
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FYC002-20P | Yeastern Biotech | 1 vial | Ask for price |
T-Pro Stacking Buffer |
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JB03-C002 | T-Pro Biotechnology | 500ml/BT | EUR 182.4 |
T-Pro MycoClean spray |
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JT90-R002 | T-Pro Biotechnology | 500ml/BT | EUR 172.8 |
T&A Cloning Kits |
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FYC001-20P | Yeastern Biotech | 1 vial | Ask for price |
T-Pro EZ Gel Solution 8% |
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JB02-B008M | T-Pro Biotechnology | 500ml/BT | EUR 266.4 |
T-Pro EZ Gel Solution 8% |
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JB02-B008S | T-Pro Biotechnology | 100ml/BT | EUR 162 |
T-Pro EZ Gel Solution 10% |
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JB02-B010M | T-Pro Biotechnology | 500ml/BT | EUR 266.4 |
T-Pro EZ Gel Solution 10% |
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JB02-B010S | T-Pro Biotechnology | 100ml/BT | EUR 162 |
T-Pro EZ Gel Solution 12% |
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JB02-B012M | T-Pro Biotechnology | 500ml/BT | EUR 266.4 |
T-Pro EZ Gel Solution 12% |
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JB02-B012S | T-Pro Biotechnology | 100ml/BT | EUR 162 |
T-Pro EZ Gel Solution 15% |
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JB02-B015M | T-Pro Biotechnology | 500ml/BT | EUR 266.4 |
T-Pro EZ Gel Solution 15% |
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JB02-B015S | T-Pro Biotechnology | 100ml/BT | EUR 162 |
T-Pro Separating or Resolving Buffer |
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JB03-C001 | T-Pro Biotechnology | 500ml/BT | EUR 182.4 |
T-Pro BCA Protein Assay kit |
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JB04-D001 | T-Pro Biotechnology | 500assay/KIT | EUR 244.8 |
T-Pro Transfer Blotting buffer (10X) |
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JB08-H001 | T-Pro Biotechnology | 500ml/BT | EUR 162 |
T-Pro Western Blot Stripping Reagent |
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JB11-K002 | T-Pro Biotechnology | 500ml/BT | EUR 213.6 |
T-Pro Aqua EZ Clean (M) |
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JT90-R001M | T-Pro Biotechnology | 500ml/BT | EUR 398.4 |
T-Pro Aqua EZ Clean (S) |
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JT90-R001S | T-Pro Biotechnology | 100ml/BT | EUR 181.2 |
T-Pro EZ stain Gel solution |
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JT90-R005M | T-Pro Biotechnology | 500ml/BT | EUR 224.4 |
T-Pro EZ stain Gel solution |
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JT90-R005S | T-Pro Biotechnology | 100ml/BT | EUR 151.2 |
T-Pro P-Fect Transfection Reagent |
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JT97-N005M | T-Pro Biotechnology | 1.0ml/vial | EUR 266.4 |
T-Pro Plasmid Mini Kit (100) |
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RB94-YPD100 | T-Pro Biotechnology | 100preps/Kit | EUR 193.2 |
T-Pro Plasmid Mini Kit (250) |
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RB94-YPD250 | T-Pro Biotechnology | 250preps/Kit | EUR 266.4 |
T-Pro Plasmid Midi Kit (20) |
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RB94-YPI020 | T-Pro Biotechnology | 20preps/Kit | EUR 255.6 |
T-Pro Plasmid Maxi Kit (10) |
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RB94-YPM010 | T-Pro Biotechnology | 10preps/Kit | EUR 266.4 |
T-Pro BCA Protein Assay Reagent A |
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JB04-D001A | T-Pro Biotechnology | 500ml/BT | EUR 204 |
T-Pro BCA Protein Assay Reagent B |
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JB04-D001B | T-Pro Biotechnology | 12ml/BT | EUR 140.4 |
T-Pro Bradford Protein Assay kit(1X) |
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JB04-D002 | T-Pro Biotechnology | 500ml/BT | EUR 193.2 |
T-Pro Fast Blocking Buffer (in PBS) |
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JK92-W001 | T-Pro Biotechnology | 500ml/BT | EUR 224.4 |
T-Pro Fast Blocking Buffer (in TBS) |
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JK92-W002 | T-Pro Biotechnology | 500ml/BT | EUR 224.4 |
T-Pro Genomic DNA Midi Kit (20) |
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RB94-NGM020 | T-Pro Biotechnology | 20preps/kit | EUR 266.4 |
T-Pro Genomic DNA Mini Kit (100) |
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RB94-NGS100 | T-Pro Biotechnology | 100preps/kit | EUR 255.6 |
Testosterone (T) ELISA Kit |
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DLR-T-Ge-48T | DL Develop | 48T | EUR 562.8 |
Description: A competitive inhibition quantitative ELISA assay kit for detection of Testosterone (T) in samples from serum, plasma, urine or other biological fluids. |
Testosterone (T) ELISA Kit |
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DLR-T-Ge-96T | DL Develop | 96T | EUR 729.6 |
Description: A competitive inhibition quantitative ELISA assay kit for detection of Testosterone (T) in samples from serum, plasma, urine or other biological fluids. |
T-Pro Laemmli (SDS sample) Reagent (reducing 4X) |
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JB06-F002 | T-Pro Biotechnology | 10ml/BT | EUR 151.2 |
T-Pro Tris-Glycine-SDS running buffer (10X) |
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JB07-G001 | T-Pro Biotechnology | 500ml/BT | EUR 162 |
T-Pro Tris-Glycine-Native running buffer (10X) |
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JB07-G002 | T-Pro Biotechnology | 500ml/BT | EUR 162 |
T-Pro Semi Dry Transfer Blotting buffer (10X) |
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JB08-H002 | T-Pro Biotechnology | 500ml/BT | EUR 172.8 |
T-Pro Protein Free Blocking Buffer (in PBS) |
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JK92-W003 | T-Pro Biotechnology | 500ml/BT | EUR 224.4 |
T-Pro Protein Free Blocking Buffer (in TBS) |
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JK92-W004 | T-Pro Biotechnology | 500ml/BT | EUR 224.4 |
T-Pro Nonliposomal Transfection Reagent I (NTR I) |
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JT97-N001M | T-Pro Biotechnology | 1.0ml/vial | EUR 224.4 |
T-Pro Nonliposomal Transfection Reagent II (NTR II) |
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JT97-N002M | T-Pro Biotechnology | 1.0ml/vial | EUR 244.8 |
T-Pro Nonliposomal Transfection Reagent III (NTR III) |
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JT97-N006M | T-Pro Biotechnology | 1.0ml/vial | EUR 266.4 |
T-Pro Endotoxin Removal Plasmid Midi kit (25) |
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RB94-EPI020 | T-Pro Biotechnology | 20preps/Kit | EUR 297.6 |
T-Pro Endotoxin Removal Plasmid Maxi kit (10) |
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RB94-EPM010 | T-Pro Biotechnology | 10preps/Kit | EUR 318 |
T-Pro Plant Genomic DNA Midi Kit (20) |
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RB94-PGM020 | T-Pro Biotechnology | 20preps/kit | EUR 266.4 |
T-Pro Plant Genomic DNA Mini Kit (100) |
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RB94-PGS100 | T-Pro Biotechnology | 100preps/kit | EUR 266.4 |
Locking T-Handle |
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M-CP-111-065-T | MiTeGen | 1 UNIT | EUR 55 |
Description: Locking T-Handle |
T-Pro Laemmli (SDS sample) Reagent (non-reducing 4X) |
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JB06-F003 | T-Pro Biotechnology | 10ml/BT | EUR 151.2 |
T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit) |
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JT96-K002M | T-Pro Biotechnology | 250ml*2/Kit | EUR 244.8 |
T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit) |
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JT96-K002S | T-Pro Biotechnology | 100ml*2/Kit | EUR 182.4 |
T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit) |
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JT96-K004M | T-Pro Biotechnology | 250ml*2/Kit | EUR 286.8 |
T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit) |
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JT96-K004S | T-Pro Biotechnology | 100ml*2/Kit | EUR 204 |
T-Pro Gel/PCR DNA Purification Maxi Kit (10) |
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RB94-NEL010 | T-Pro Biotechnology | 10preps/Kit | EUR 193.2 |
T-Pro Gel/PCR DNA Purification Mini Kit (100) |
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RB94-NES100 | T-Pro Biotechnology | 100preps/Kit | EUR 193.2 |
T-Pro Gel/PCR DNA Purification Mini Kit (250) |
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RB94-NES250 | T-Pro Biotechnology | 250preps/Kit | EUR 266.4 |
General Testosterone (T) ELISA Kit |
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RD-T-Ge-48Tests | Reddot Biotech | 48 Tests | EUR 560.4 |
General Testosterone (T) ELISA Kit |
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RD-T-Ge-96Tests | Reddot Biotech | 96 Tests | EUR 775.2 |
General Testosterone (T) ELISA Kit |
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RDR-T-Ge-48Tests | Reddot Biotech | 48 Tests | EUR 585.6 |
General Testosterone (T) ELISA Kit |
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RDR-T-Ge-96Tests | Reddot Biotech | 96 Tests | EUR 811.2 |
T-Pro Washing buffer in PBS and Tween-20 (10X) |
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JB09-I003 | T-Pro Biotechnology | 500ml/BT | EUR 172.8 |
T-Pro Washing buffer in TBS and Tween-20 (10X) |
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JB09-I004 | T-Pro Biotechnology | 500ml/BT | EUR 172.8 |
T&A Cloning Kit + ECOS 101DH5a |
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FYC005-20P | Yeastern Biotech | 1 vial | Ask for price |
T-Pro Phosphate-Buffered Saline (PBS, 10X) for western blot washing |
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JB09-I001 | T-Pro Biotechnology | 500ml/BT | EUR 162 |
T-Pro Tris-Buffered Saline (TBS, 10X) for western blot washing |
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JB09-I002 | T-Pro Biotechnology | 500ml/BT | EUR 162 |
pET His6 Sumo TEV LIC cloning vector (2S-T) |
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PVT14035 | Lifescience Market | 2 ug | EUR 718.8 |
T&A Cloning Kit + ECOS 9-5JM109 |
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FYC006-20P | Yeastern Biotech | 1 vial | Ask for price |
Vector T Plasmid |
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PVT7016 | Lifescience Market | 2 ug | EUR 314.4 |
siRNA Cloning Vector (pGB) |
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9500-20 | Biovision | EUR 352.8 |
T ORF Vector (Rat) (pORF) |
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ORF077342 | ABM | 1.0 ug DNA | EUR 607.2 |
T ORF Vector (Mouse) (pORF) |
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ORF058986 | ABM | 1.0 ug DNA | EUR 607.2 |
T ORF Vector (Human) (pORF) |
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ORF033613 | ABM | 1.0 ug DNA | EUR 486 |
Transcription factor T (T) polyclonal antibody |
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ABP-PAB-11172 | Allele Biotech | 100 ug | Ask for price |
Transcription factor T (T) polyclonal antibody |
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ABP-PAB-11173 | Allele Biotech | 100 ug | Ask for price |
Immortalized Hamster Hepatocytes-SV40 T+t |
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T0071 | ABM | 1x106 cells / 1.0 ml | Ask for price |
Immortalized Monkey Hepatocytes-SV40 T+t |
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T0074 | ABM | 1x106 cells / 1.0 ml | Ask for price |
T. gondii |
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DAG1494 | Creative Diagnostics | 100 µg | EUR 774 |
T. gondii |
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DAG1495 | Creative Diagnostics | 100 µg | EUR 774 |
T. gondii |
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DAG1496 | Creative Diagnostics | 100 µg | EUR 774 |
T siRNA |
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20-abx938300 | Abbexa |
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T siRNA |
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20-abx938301 | Abbexa |
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T Antibody |
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48451-100ul | SAB | 100ul | EUR 399.6 |
T Antibody |
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48451-50ul | SAB | 50ul | EUR 286.8 |
Peptide T |
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5-01748 | CHI Scientific | 4 x 5mg | Ask for price |
DMT-T |
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5-02235 | CHI Scientific | 5g | Ask for price |
DMT-T |
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5-02236 | CHI Scientific | 25g | Ask for price |
Thioflavine T |
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20-abx184437 | Abbexa |
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T-5224 |
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9546-25 | Biovision | EUR 823.2 |
T-5224 |
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9546-5 | Biovision | EUR 261.6 |
T Antibody |
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BF0015 | Affbiotech | 200ul | EUR 540 |
T-5224 |
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B4664-10 | ApexBio | 10 mg | EUR 268.8 |
Description: T-5224 is a non-peptidic, small molecule and novel inhibitor of c-Fos/AP-1 [1].T-5224 has shown the specific inhibition in a c-Fos/AP-1promoter-luciferase assay, without affecting the levels of c-Fos family protein members themselves. |
T-5224 |
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B4664-100 | ApexBio | 100 mg | EUR 1104 |
Description: T-5224 is a non-peptidic, small molecule and novel inhibitor of c-Fos/AP-1 [1].T-5224 has shown the specific inhibition in a c-Fos/AP-1promoter-luciferase assay, without affecting the levels of c-Fos family protein members themselves. |
T-5224 |
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B4664-5 | ApexBio | 5 mg | EUR 199.2 |
Description: T-5224 is a non-peptidic, small molecule and novel inhibitor of c-Fos/AP-1 [1].T-5224 has shown the specific inhibition in a c-Fos/AP-1promoter-luciferase assay, without affecting the levels of c-Fos family protein members themselves. |
T-5224 |
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B4664-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 212.4 |
Description: T-5224 is a non-peptidic, small molecule and novel inhibitor of c-Fos/AP-1 [1].T-5224 has shown the specific inhibition in a c-Fos/AP-1promoter-luciferase assay, without affecting the levels of c-Fos family protein members themselves. |
T-5224 |
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B4664-50 | ApexBio | 50 mg | EUR 686.4 |
Description: T-5224 is a non-peptidic, small molecule and novel inhibitor of c-Fos/AP-1 [1].T-5224 has shown the specific inhibition in a c-Fos/AP-1promoter-luciferase assay, without affecting the levels of c-Fos family protein members themselves. |
T 5601640 |
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B4940-10 | ApexBio | 10 mg | EUR 286.8 |
T 5601640 |
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B4940-100 | ApexBio | 100 mg | EUR 1537.2 |
T 5601640 |
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B4940-25 | ApexBio | 25 mg | EUR 537.6 |
T 5601640 |
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B4940-5 | ApexBio | 5 mg | EUR 189.6 |
T 5601640 |
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B4940-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 201.6 |
T 5601640 |
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B4940-50 | ApexBio | 50 mg | EUR 907.2 |
Conantokin-T |
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B5378-.5 | ApexBio | 500 ug | EUR 673.2 |
T 705 |
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B1114-25 | ApexBio | 25 mg | EUR 448.8 |
Description: T 705(Favipiravir) is a RNA-directed RNA polymerase NS5B inhibitor. |
T 705 |
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B1114-5 | ApexBio | 5 mg | EUR 164.4 |
Description: T 705(Favipiravir) is a RNA-directed RNA polymerase NS5B inhibitor. |
T 705 |
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B1114-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 129.6 |
Description: T 705(Favipiravir) is a RNA-directed RNA polymerase NS5B inhibitor. |
T 98475 |
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B7090-1 | ApexBio | 1 mg | EUR 525.6 |
T 82 |
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HY-U00028 | MedChemExpress | 5mg | EUR 3415.2 |
T-1105 |
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HY-W015764 | MedChemExpress | 10mM/1mL | EUR 135.6 |
Chloramine-T |
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HY-B0959 | MedChemExpress | 1g | EUR 142.8 |
Thioflavin T |
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HY-D0218 | MedChemExpress | 10mM/1mL | EUR 135.6 |
Peptide T |
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HY-P0272 | MedChemExpress | 5mg | EUR 362.4 |
T-00127_HEV1 |
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HY-108313 | MedChemExpress | 1mg | EUR 224.4 |
T-448 |
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HY-122635A | MedChemExpress | 1mg | EUR 817.2 |
T-5224 |
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HY-12270 | MedChemExpress | 100mg | EUR 1282.8 |
T-Peptide |
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H-8126.0500 | Bachem | 0.5mg | EUR 352.8 |
Description: Sum Formula: C92H171N45O18; CAS# [2022956-62-1] net |
T-Peptide |
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H-8126.1000 | Bachem | 1.0mg | EUR 572.4 |
Description: Sum Formula: C92H171N45O18; CAS# [2022956-62-1] net |
T-26c |
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HY-100518 | MedChemExpress | 10mM/1mL | EUR 241.2 |
Esculentoside T |
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TB0765-0050 | ChemNorm | 10mg | EUR 325.2 |
Gomisin T |
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TBZ0382 | ChemNorm | unit | Ask for price |
dCas9 C-terminal Cloning Vector |
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K014 | ABM | 10 ug | EUR 865.2 |
HLA-T ORF Vector (Human) (pORF) |
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ORF020809 | ABM | 1.0 ug DNA | Ask for price |
Mouse Troponin T(Tn-T)ELISA Kit       |
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GA-E0198MS-48T | GenAsia Biotech | 48T | EUR 403.2 |
Mouse Troponin T(Tn-T)ELISA Kit       |
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GA-E0198MS-96T | GenAsia Biotech | 96T | EUR 640.8 |
Rat Troponin T(Tn-T)ELISA Kit |
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GA-E0314RT-48T | GenAsia Biotech | 48T | EUR 380.4 |
Rat Troponin T(Tn-T)ELISA Kit |
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GA-E0314RT-96T | GenAsia Biotech | 96T | EUR 595.2 |
Human Troponin T (Tn-T) CLIA Kit |
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20-abx493117 | Abbexa |
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Rabbit Troponin T (Tn-T) ELISA Kit |
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abx257093-96tests | Abbexa | 96 tests | EUR 990 |
Human Troponin T,Tn-T ELISA Kit |
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201-12-1255 | SunredBio | 96 tests | EUR 528 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Recombinant human T-box transcription factor T |
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P1534 | FN Test | 100ug | Ask for price |
Description: Recombinant protein for human T-box transcription factor T |
Tn-T/ Rat Tn- T ELISA Kit |
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ELA-E1820r | Lifescience Market | 96 Tests | EUR 1063.2 |
Human Troponin T(Tn-T)ELISA Kit |
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GA-E1271HM-48T | GenAsia Biotech | 48T | EUR 346.8 |
Human Troponin T(Tn-T)ELISA Kit |
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GA-E1271HM-96T | GenAsia Biotech | 96T | EUR 559.2 |
Rat Troponin T(Tn-T)ELISA Kit |
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QY-E11338 | Qayee Biotechnology | 96T | EUR 433.2 |
Mouse Troponin T(Tn-T)ELISA Kit |
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QY-E21529 | Qayee Biotechnology | 96T | EUR 433.2 |
Human Troponin T(Tn-T)ELISA Kit |
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QY-E03068 | Qayee Biotechnology | 96T | EUR 433.2 |
Portex T Connector T 9mm o d |
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CON7202 | Scientific Laboratory Supplies | PK10 | EUR 34.2 |
Portex T Connector T 11mm o d |
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CON7204 | Scientific Laboratory Supplies | PK10 | EUR 43.32 |
Exosome precipitation from Plasma + Thrombin |
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EPStep-T | ImmunoStep | 5 ml | EUR 403.2 |
Live-or-Dye UV/448 Fixable Viability Staining Kit, Trial Size (50 assays) |
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32002-T | Biotium | 1kit | EUR 144 |
Description: Minimum order quantity: 1 unit of 1kit |
Live-or-Dye 405/452 Fixable Viability Staining Kit, Trial Size (50 assays) |
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32003-T | Biotium | 1kit | EUR 144 |
Description: Minimum order quantity: 1 unit of 1kit |
Live-or-Dye 488/515 Fixable Viability Staining Kit, Trial Size (50 assays) |
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32004-T | Biotium | 1kit | EUR 144 |
Description: Minimum order quantity: 1 unit of 1kit |