Adeno-associated virus (AAV) vectors are at present among the many mostly utilized for in vivo gene remedy approaches. The analysis of vectors throughout medical improvement requires the manufacturing of appreciable quantities of extremely pure and potent vectors. Right here, we set up a scalable course of for AAV manufacturing, utilizing orbitally shaken bioreactors and a completely characterised suspension-adapted cell line, HEKExpress. We carried out a proof-of-concept manufacturing of AAV2/eight and AAV2/9 vectors utilizing HEKExpress cells.
Moreover, we in contrast the manufacturing of AAV2/9 vectors utilizing this suspension cell line to classical protocols based mostly on adherent HEK293 cells to show bioequivalence in vitro and in vivo. Following upstream processing, we purified vectors by way of gradient centrifugation and immunoaffinity chromatography. The in vitro characterization revealed variations because of the purification methodology, in addition to the transfection protocol and the corresponding HEK293 cell line. The purification methodology and cell line used additionally affected in vivo transduction effectivity after bilateral injection of AAV2/9 vectors expressing a GFP reporter fused with a nuclear localization sign (AAV2/9-CBA-nlsGFP) into the striatum of grownup mice.
These outcomes present that AAV vectors deriving from suspension HEKExpress cells are bioequivalent and should exhibit larger efficiency than vectors produced with adherent HEK293 cells.
Lengthy-Vary, Border-Crossing, Horizontal Axon Radiations Are a Widespread Function of Rat Neocortical Areas That Differ in Cytoarchitecture.
Using wide-field optical imaging strategies supported by electrophysiological recordings, earlier research have demonstrated that stimulation of a spatially restricted space (level) within the sensory periphery leads to a big evoked neuronal exercise unfold in mammalian main cortices. In rats’ main cortices, such giant evoked spreads prolong diffusely in a number of instructions, cross cortical cytoarchitectural borders and might trespass into different unimodal sensory areas. These level spreads are supported by a spatially matching, diffuse set of long-range horizontal projections inside grey matter that stretch in a number of instructions and cross borders to interconnect totally different cortical areas.
This horizontal projection system is along with well-known area-to-area clustered projections to outlined targets by way of white matter. May related two-projection cortical programs even be present in cortical areas that differ of their cytoarchitectural construction? To deal with this query, an adeno-associated viral vector expressing inexperienced fluorescent protein (GFP) was injected as an anterograde tract tracer into granular somatosensory cortex (trunk space), dysgranular cortex (somatosensory dysgranular zone and extrastriate cortex) and agranular motor cortex (MCx). Regardless of the injection website the identical two projection programs had been discovered, and their quantification revealed an in depth similarity to findings in main sensory cortices. Following detailed reconstruction, the diffuse horizontal axon radiation was discovered to own quite a few varicosities and to incorporate brief, medium and lengthy axons, the latter extending as much as 5.2 mm.
These “proof of idea” findings recommend that the similarity of the 2 projection programs amongst totally different cortical areas may doubtlessly represent a canonical motif of neocortical group. Monitoring the habits of leukemic samples each in vitro and in vivo performs an more and more giant function in efforts to raised perceive the leukemogenic processes and the results of potential new therapies. Such work might be accelerated and made extra environment friendly by methodologies enabling the characterization of leukemia samples in multiplex assays. We not too long ago developed three units of lentiviral fluorescent genetic barcoding (FGB) vectors that create 26, 14, and 6 distinctive immunophenotyping-compatible shade codes from GFP-, yellow fluorescent protein (YFP)-, and monomeric kusabira orange 2 (mKO2)-derived fluorescent proteins.
These vectors permit for labeling and monitoring of particular person color-coded cell populations in combined samples by real-time stream cytometry. Utilizing the prototypical Hoxa9/Meis1 murine mannequin of acute myeloid leukemia, we describe the appliance of the 6xFGB vector system for assessing leukemic cell traits in multiplex assays.
Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells.
Simplified inducible system for Trypanosoma brucei.
These days, most reverse genetics approaches in Trypanosoma brucei, a protozoan parasite of medical and veterinary significance, depend on pre-established cell strains. Consequently, inducible experimentation is diminished to some laboratory strains. Right here we described a brand new transgene expression system based mostly completely on endogenous transcription actions and a minimal set of regulatory elements that may simply been tailored to totally different strains. The pTbFIX vectors are designed to include the sequence of curiosity below the management of an inducible rRNA promoter together with a constitutive dicistronic unit encoding a nucleus focused tetracycline repressor and puromycin resistance genes in a tandem “head-to-tail” configuration. Upon doxycycline induction, the system helps regulatable GFP expression (170 to 400 fold) in each bloodstream and procyclic T. brucei kinds.
Moreover we’ve tailored the pTbFIX plasmid to carry out RNAi experimentation. Deadly phenotypes, together with α-tubulin and people akin to the enolase and clathrin heavy chain genes, had been efficiently recapitulated in procyclic and bloodstream parasites thus displaying the flexibility of this new device. we arrange CUG-expansion and Celf1 overexpression C2C12 cell fashions to mimic the myocyte differentiation defects of DM1, then transfected AdvmiR-206 into cell fashions, examined the extent of myogenic markers MyoD, MyoG, Mef2c, Celf1 by RT-PCRand Western Blotting, detected myotube formation by myosin heavy chain immunostaining. Consequence: 3′-UTR CUG-expansion results in myotube defects and impaired myoblasts differentiation.
T&A Cloning Vector |
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| FYC002-20P | Yeastern Biotech | 1 vial | Ask for price |
pTG19-T PCR Cloning Vector, 20apps |
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| TA010 | Vivantis | each | Ask for price |
pET His6 Sumo TEV LIC cloning vector (2S-T) |
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| PVT14035 | Lifescience Market | 2 ug | EUR 718.8 |
siRNA Cloning Vector (pGB) |
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| 9500-20 | Biovision | each | EUR 352.8 |
dCas9 C-terminal Cloning Vector |
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| K014 | ABM | 10 ug | EUR 545 |
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Description: N/A |
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pcDNA3 GFP LIC cloning vector (6D) |
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| PVT10748 | Lifescience Market | 2 ug | EUR 361.2 |
pLenti-III-mir-GFP Cloning Vector |
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| m016 | ABM | 500 ng | EUR 725 |
pET His6 MBP TEV LIC cloning vector (1M) |
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| PVT10596 | Lifescience Market | 2 ug | EUR 319.2 |
pET His6 Sumo TEV LIC cloning vector (1S) |
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| PVT14034 | Lifescience Market | 2 ug | EUR 718.8 |
pFastBac Dual His6 TEV LIC cloning vector(5B) Plasmid |
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| PVT43314 | Nova Lifetech | 2ug | EUR 280 |
pFastBac His6 TEV cloning vector with BioBrick polycistronic |
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| PVT29881 | Nova Lifetech | 2ug | EUR 280 |
pCDF1-MCS1 cDNA Cloning and Expression Vector |
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| CD100A-1 | SBI | 10 ug | EUR 483 |
pCDH-CMV-MCS cDNA Cloning and Expression Vector |
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| CD500B-1 | SBI | 10 ug | EUR 483 |
pCDH-EF1-MCS cDNA Cloning and Expression Vector |
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| CD502A-1 | SBI | 10 ug | EUR 483 |
T&A Cloning Kits |
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| FYC001-20P | Yeastern Biotech | 1 vial | Ask for price |
pCDH-CMV-MCS2 cDNA Cloning and Expression Vector |
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| CD501A-1 | SBI | 10 ug | EUR 483 |
pSIH1-H1-Puro shRNA Cloning and Expression Vector |
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| SI500A-1 | SBI | 10 ug | EUR 507 |
pSIH1-H1-H2Kk shRNA Cloning and Expression Vector |
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| SI502A-1 | SBI | 10 ug | EUR 507 |
pAAVK-EF1α -MCS AAVanced Cloning and Expression Vector |
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| AAV502A-1 | SBI | 10 ug | EUR 614 |
pSIH1-H1-copGFP shRNA Cloning and Expression Vector |
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| SI501A-1 | SBI | 10 ug | EUR 507 |
Cumate-Inducible (CuO) EEV cloning and expression vector |
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| EEV610A-1 | SBI | 10 ug | EUR 614 |
Green Kit. Baculovirus GFP vector. |
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| K20 | AB Vector LLC | 1 Kit | EUR 695 |
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Description: Protein expression |
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ProGreen. Baculovirus GFP marker vector. |
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| A1 | AB Vector LLC | 25 ul | EUR 420 |
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Description: Protein expression |
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pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector |
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| CD811A-1 | SBI | 10 ug | EUR 579 |
pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector |
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| CD810A-1 | SBI | 10 ug | EUR 579 |
Constitutive (CAGs promoter) EEV cloning and expression vector |
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| EEV600A-1 | SBI | 10 ug | EUR 614 |
pCDH-MCS-T2A-copGFP-MSCV Cloning and Expression Vector |
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| CD523A-1 | SBI | 10 ug | EUR 590 |
pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector |
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| CD111B-1 | SBI | 10 ug | EUR 572 |
PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector |
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| PB511B-1 | SBI | 10 ug | EUR 638 |
PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector |
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| PB512B-1 | SBI | 10 ug | EUR 638 |
PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector |
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| PB510B-1 | SBI | 10 ug | EUR 638 |
PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector |
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| PB530A-2 | SBI | 10 ug | EUR 638 |
PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
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| PB531A-2 | SBI | 10 ug | EUR 638 |
PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector |
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| PB533A-2 | SBI | 10 ug | EUR 638 |
pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector |
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| CD512B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector |
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| CD514B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
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| CD510B-1 | SBI | 10 ug | EUR 572 |
pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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| CD530A-2 | SBI | 10 ug | EUR 590 |
pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
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| CD531A-2 | SBI | 10 ug | EUR 590 |
pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector |
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| CD533A-2 | SBI | 10 ug | EUR 590 |
pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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| CD630A-1 | SBI | 10 ug | EUR 590 |
pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector |
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| CD711B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
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| CD515B-1 | SBI | 10 ug | EUR 572 |
pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector |
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| CD522A-1 | SBI | 10 ug | EUR 590 |
pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector |
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| CD532A-2 | SBI | 10 ug | EUR 590 |
pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
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| CD615B-1 | SBI | 10 ug | EUR 572 |
pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
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| CD710B-1 | SBI | 10 ug | EUR 572 |
pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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| CD731B-1 | SBI | 10 ug | EUR 590 |
PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector |
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| PB514B-2 | SBI | 10 ug | EUR 638 |
ProEasy. Vector for easy construction of recombinant baculoviruses. |
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| A10S | AB Vector LLC | 25 ul | EUR 695 |
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Description: Protein expression |
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pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector |
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| CD511B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector |
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| CD524A-1 | SBI | 10 ug | EUR 590 |
PinPoint-HR attP Placement Vector – Empty MCS for cloning HR Arms |
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| PIN400A-1 | SBI | 10ug | EUR 1190 |
PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector |
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| PB513B-1 | SBI | 10 ug | EUR 638 |
PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector |
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| PBSI505A-1 | SBI | 10 ug | EUR 638 |
PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector |
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| PBSI506A-1 | SBI | 10 ug | EUR 638 |
pAAVK-EF1α -MCS-T2A-EGFP AAVanced Cloning and Expression Vector |
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| AAV526A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α -MCS-T2A-Puro AAVanced Cloning and Expression Vector |
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| AAV527A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α –MCS-T2A-mRFP AAVanced Cloning and Expression Vector |
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| AAV528A-1 | SBI | 10 ug | EUR 614 |
PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
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| PB713B-1 | SBI | 10 ug | EUR 638 |
pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
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| CD513B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector |
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| CD516B-2 | SBI | 10 ug | EUR 572 |
pAB-bee. Baculovirus plasmid vector for secreted and transmembrane proteins. |
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| B3 | AB Vector LLC | 50 ul | EUR 495 |
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Description: Protein expression |
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pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector |
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| CD713B-1 | SBI | 10 ug | EUR 572 |
pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector |
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| CD813A-1 | SBI | 10 ug | EUR 579 |
pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector |
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| CD822A-1 | SBI | 10 ug | EUR 579 |
pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector |
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| CD823A-1 | SBI | 10 ug | EUR 579 |
pAAVK-EF1α-MCS1-CMV-MCS2 AAVanced Cloning and Expression Vector |
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| AAV503A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α-MCS1-CMV-EGFP AAVanced Cloning and Expression Vector |
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| AAV536A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α-MCS1-CMV-Puro AAVanced Cloning and Expression Vector |
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| AAV537A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α-MCS1-CMV-mRFP AAVanced Cloning and Expression Vector |
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| AAV538A-1 | SBI | 10 ug | EUR 614 |
T&A Cloning Kit + ECOS 101DH5a |
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| FYC005-20P | Yeastern Biotech | 1 vial | Ask for price |
pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 µg |
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| CD527A-1 | SBI | 10 ug | EUR 590 |
ProFold-PDI. Baculovirus chaperone vector for expression of cysteine-rich proteins. |
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| A7 | AB Vector LLC | 25 ul | EUR 830 |
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Description: Protein expression |
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gRNA_Cloning Vector |
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| PVTY00131 | Nova Lifetech | 2ug | EUR 280 |
ProFold-C1. Baculovirus chaperone vector for expression of cytoplasmic and nuclear proteins. |
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| A2 | AB Vector LLC | 25 ul | EUR 830 |
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Description: Protein expression |
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ProFold-C2. Baculovirus chaperone vector for expression of cytoplasmic and nuclear proteins. |
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| A3 | AB Vector LLC | 25 ul | EUR 830 |
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Description: Protein expression |
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ProFold-ER1. Baculovirus chaperone vector for expression of secreted and membrane proteins. |
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| A4 | AB Vector LLC | 25 ul | EUR 830 |
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Description: Protein expression |
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T&A Cloning Kit + ECOS 9-5JM109 |
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| FYC006-20P | Yeastern Biotech | 1 vial | Ask for price |
pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector |
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| CD550A-1 | SBI | 10 ug | EUR 563 |
PB-CuO-CMV-MCS-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector |
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| PBQM800A-1 | SBI | 10 µg | EUR 921 |
PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector |
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| PBQM812A-1 | SBI | 10 ug | EUR 1023 |
Cloning |
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| S201 | 101Bio | - | Ask for price |
PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector |
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| PBQMSH812A-1 | SBI | 10 ug | EUR 1023 |
T-Pro MycoClean spray |
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| JT90-R002 | T-Pro Biotechnology | 500ml/BT | EUR 60 |
T-Pro Stacking Buffer |
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| JB03-C002 | T-Pro Biotechnology | 500ml/BT | EUR 40 |
T-Pro Blocking Buffer |
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| JK92-W005 | T-Pro Biotechnology | 500ml/BT | EUR 60 |
C1 Kit. Baculovirus chaperone vectors for cytoplasmic and nuclear proteins. |
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| K21 | AB Vector LLC | 1 Kit | EUR 995 |
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Description: Protein expression |
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C2 Kit. Baculovirus chaperone vectors for cytoplasmic and nuclear proteins. |
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| K22 | AB Vector LLC | 1 Kit | EUR 995 |
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Description: Protein expression |
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ER1 Kit. Baculovirus chaperone vectors for expression of secreted and membrane proteins. |
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| K23 | AB Vector LLC | 1 Kit | EUR 995 |
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Description: Protein expression |
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ER1-bee Kit. Baculovirus chaperone vectors for expression of secreted and membrane proteins. |
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| K24 | AB Vector LLC | 1 Kit | EUR 995 |
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Description: Protein expression |
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T-Pro RIPA Lysis buffer (1X) |
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| JT89-L001M | T-Pro Biotechnology | 500ml/BT | EUR 60 |
T-Pro RIPA Lysis buffer (5X) |
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| JT89-L005S | T-Pro Biotechnology | 100ml/BT | EUR 60 |
T-Pro Aqua EZ Clean (M) |
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| JT90-R001M | T-Pro Biotechnology | 500ml/BT | EUR 200 |
T-Pro Aqua EZ Clean (S) |
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| JT90-R001S | T-Pro Biotechnology | 100ml/BT | EUR 60 |
T-Pro EZ stain Gel solution |
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| JT90-R005S | T-Pro Biotechnology | 100ml/BT | EUR 151.2 |
T-Pro Ponceau S stain solution |
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| JT90-R006M | T-Pro Biotechnology | 500ml/BT | EUR 60 |
T-Pro LumiFast Plus ECL Kit |
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| JT96-K002M | T-Pro Biotechnology | 250ml*2/Kit | EUR 120 |
T-Pro LumiFast Plus ECL Kit |
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| JT96-K002S | T-Pro Biotechnology | 100ml*2/Kit | EUR 60 |
T-Pro P-Fect Transfection Reagent |
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| JT97-N005M | T-Pro Biotechnology | 1.0ml/vial | EUR 266.4 |
T-Pro EZ Gel Solution 6% |
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| JB02-B006M | T-Pro Biotechnology | 500ml/BT | EUR 120 |
T-Pro EZ Gel Solution 6% |
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| JB02-B006S | T-Pro Biotechnology | 100ml/BT | EUR 30 |
T-Pro EZ Gel Solution 8% |
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| JB02-B008M | T-Pro Biotechnology | 500ml/BT | EUR 120 |
T-Pro EZ Gel Solution 8% |
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| JB02-B008S | T-Pro Biotechnology | 100ml/BT | EUR 30 |
T-Pro EZ Gel Solution 10% |
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| JB02-B010M | T-Pro Biotechnology | 500ml/BT | EUR 120 |
T-Pro EZ Gel Solution 10% |
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| JB02-B010S | T-Pro Biotechnology | 100ml/BT | EUR 30 |
T-Pro EZ Gel Solution 12% |
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| JB02-B012M | T-Pro Biotechnology | 500ml/BT | EUR 120 |
T-Pro EZ Gel Solution 12% |
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| JB02-B012S | T-Pro Biotechnology | 100ml/BT | EUR 30 |
T-Pro EZ Gel Solution 15% |
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| JB02-B015M | T-Pro Biotechnology | 500ml/BT | EUR 120 |
T-Pro EZ Gel Solution 15% |
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| JB02-B015S | T-Pro Biotechnology | 100ml/BT | EUR 30 |
T-Pro Separating or Resolving Buffer |
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| JB03-C001 | T-Pro Biotechnology | 500ml/BT | EUR 40 |
T-Pro BCA Protein Assay kit |
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| JB04-D001 | T-Pro Biotechnology | 500assay/KIT | EUR 120 |
T-Pro Transfer Blotting buffer (10X) |
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| JB08-H001 | T-Pro Biotechnology | 500ml/BT | EUR 40 |
T-Pro Western Blot Stripping Reagent |
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| JB11-K002 | T-Pro Biotechnology | 500ml/BT | EUR 60 |
T-Pro Cell Counting Kit (CCK8) |
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| JK95-C008L | T-Pro Biotechnology | 5ml*20/BT | EUR 1000 |
T-Pro Cell Counting Kit (CCK8) |
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| JK95-C008M | T-Pro Biotechnology | 5ml*2/BT | EUR 120 |
T-Pro Cell Counting Kit (CCK8) |
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| JK95-C008S | T-Pro Biotechnology | 1ml*5/BT | EUR 80 |
T-Pro Plasmid Mini Kit (100) |
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| RB94-YPD100 | T-Pro Biotechnology | 100preps/Kit | EUR 193.2 |
T-Pro Plasmid Mini Kit (250) |
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| RB94-YPD250 | T-Pro Biotechnology | 250preps/Kit | EUR 266.4 |
T-Pro Plasmid Midi Kit (20) |
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| RB94-YPI020 | T-Pro Biotechnology | 20preps/Kit | EUR 255.6 |
T-Pro Plasmid Maxi Kit (10) |
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| RB94-YPM010 | T-Pro Biotechnology | 10preps/Kit | EUR 266.4 |
gRNA- Cloning |
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| PVT10957 | Lifescience Market | 2 ug | EUR 361.2 |
T-Pro EZ Protein Gel stain solution |
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| JT90-R005M | T-Pro Biotechnology | 500ml/BT | EUR 60 |
T-Pro BCA Protein Assay Reagent A |
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| JB04-D001A | T-Pro Biotechnology | 500ml/BT | EUR 100 |
T-Pro BCA Protein Assay Reagent B |
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| JB04-D001B | T-Pro Biotechnology | 12ml/BT | EUR 20 |
T-Pro Bradford Protein Assay kit (1X) |
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| JB04-D002 | T-Pro Biotechnology | 500ml/BT | EUR 30 |
T-Pro Bradford Protein Assay kit (5X) |
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| JB04-D003 | T-Pro Biotechnology | 500ml/BT | EUR 120 |
T-Pro MOPS/SDS running buffer (20X) |
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| JB07-G003 | T-Pro Biotechnology | 500ml/BT | EUR 80 |
T-Pro HQ Transfer Blotting buffer (20X) |
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| JB08-H003 | T-Pro Biotechnology | 500ml/BT | EUR 80 |
T-Pro Western Blot Stripping Reagent (EX) |
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| JB11-K005 | T-Pro Biotechnology | 500ml/BT | EUR 60 |
T-Pro MOPS buffer (1M, pH 6.5) |
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| JB12-S001 | T-Pro Biotechnology | 500ml/BT | EUR 60 |
T-Pro MOPS buffer (1M, pH 7.0) |
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| JB12-S002 | T-Pro Biotechnology | 500ml/BT | EUR 60 |
T-Pro MOPS buffer (1M, pH 7.5) |
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| JB12-S003 | T-Pro Biotechnology | 500ml/BT | EUR 60 |
T-Pro Fast Blocking Buffer (in PBS) |
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| JK92-W001 | T-Pro Biotechnology | 500ml/BT | EUR 60 |
T-Pro Fast Blocking Buffer (in TBS) |
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| JK92-W002 | T-Pro Biotechnology | 500ml/BT | EUR 60 |
T-Pro Genomic DNA Midi Kit (20) |
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| RB94-NGM020 | T-Pro Biotechnology | 20preps/kit | EUR 266.4 |
T-Pro Genomic DNA Mini Kit (100) |
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| RB94-NGS100 | T-Pro Biotechnology | 100preps/kit | EUR 255.6 |
Cloning Optimizer |
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| E004 | ABM | 200 μl | EUR 105 |
|
Description: Cloning Optimizer consists of a proprietary enzyme that can completely eliminate background colonies when cloning. Treatment of PCR amplified DNA with this innovative optimizer will remove the trace amounts of plasmid template DNA that remain after PCR. Cloning Optimizer eliminates non-linearized template plasmids that cause false-positive colonies. |
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Cloning Optimizer |
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| MBS4156417-02mL | MyBiosource | 0.2mL | EUR 245 |
Cloning Optimizer |
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| MBS4156417-5x02mL | MyBiosource | 5x0.2mL | EUR 795 |
T-Pro LumiDura Chemiluminescence Detection Kit (ECL Kit) |
|||
| JT96-K006M | T-Pro Biotechnology | 250ml*2/Kit | EUR 200 |
Overexpression of Celf1 inhibits myoblast differentiation and impairs differentiation. Knockdown of Celf1 partially rescues differentiation defects of myoblasts harboring CUG-expansion. miR-206 incompletely reverses myoblast differentiation inhibition induced by CUG-expansion and partially recuses myoblast differentiation defects induced by Celf1 overexpression.