Adeno-associated virus (AAV) vectors are at present among the many mostly utilized for in vivo gene remedy approaches. The analysis of vectors throughout medical improvement requires the manufacturing of appreciable quantities of extremely pure and potent vectors. Right here, we set up a scalable course of for AAV manufacturing, utilizing orbitally shaken bioreactors and a completely characterised suspension-adapted cell line, HEKExpress. We carried out a proof-of-concept manufacturing of AAV2/eight and AAV2/9 vectors utilizing HEKExpress cells.
Moreover, we in contrast the manufacturing of AAV2/9 vectors utilizing this suspension cell line to classical protocols based mostly on adherent HEK293 cells to show bioequivalence in vitro and in vivo. Following upstream processing, we purified vectors by way of gradient centrifugation and immunoaffinity chromatography. The in vitro characterization revealed variations because of the purification methodology, in addition to the transfection protocol and the corresponding HEK293 cell line. The purification methodology and cell line used additionally affected in vivo transduction effectivity after bilateral injection of AAV2/9 vectors expressing a GFP reporter fused with a nuclear localization sign (AAV2/9-CBA-nlsGFP) into the striatum of grownup mice.
These outcomes present that AAV vectors deriving from suspension HEKExpress cells are bioequivalent and should exhibit larger efficiency than vectors produced with adherent HEK293 cells.
Lengthy-Vary, Border-Crossing, Horizontal Axon Radiations Are a Widespread Function of Rat Neocortical Areas That Differ in Cytoarchitecture.
Using wide-field optical imaging strategies supported by electrophysiological recordings, earlier research have demonstrated that stimulation of a spatially restricted space (level) within the sensory periphery leads to a big evoked neuronal exercise unfold in mammalian main cortices. In rats’ main cortices, such giant evoked spreads prolong diffusely in a number of instructions, cross cortical cytoarchitectural borders and might trespass into different unimodal sensory areas. These level spreads are supported by a spatially matching, diffuse set of long-range horizontal projections inside grey matter that stretch in a number of instructions and cross borders to interconnect totally different cortical areas.
This horizontal projection system is along with well-known area-to-area clustered projections to outlined targets by way of white matter. May related two-projection cortical programs even be present in cortical areas that differ of their cytoarchitectural construction? To deal with this query, an adeno-associated viral vector expressing inexperienced fluorescent protein (GFP) was injected as an anterograde tract tracer into granular somatosensory cortex (trunk space), dysgranular cortex (somatosensory dysgranular zone and extrastriate cortex) and agranular motor cortex (MCx). Regardless of the injection website the identical two projection programs had been discovered, and their quantification revealed an in depth similarity to findings in main sensory cortices. Following detailed reconstruction, the diffuse horizontal axon radiation was discovered to own quite a few varicosities and to incorporate brief, medium and lengthy axons, the latter extending as much as 5.2 mm.
These “proof of idea” findings recommend that the similarity of the 2 projection programs amongst totally different cortical areas may doubtlessly represent a canonical motif of neocortical group. Monitoring the habits of leukemic samples each in vitro and in vivo performs an more and more giant function in efforts to raised perceive the leukemogenic processes and the results of potential new therapies. Such work might be accelerated and made extra environment friendly by methodologies enabling the characterization of leukemia samples in multiplex assays. We not too long ago developed three units of lentiviral fluorescent genetic barcoding (FGB) vectors that create 26, 14, and 6 distinctive immunophenotyping-compatible shade codes from GFP-, yellow fluorescent protein (YFP)-, and monomeric kusabira orange 2 (mKO2)-derived fluorescent proteins.
These vectors permit for labeling and monitoring of particular person color-coded cell populations in combined samples by real-time stream cytometry. Utilizing the prototypical Hoxa9/Meis1 murine mannequin of acute myeloid leukemia, we describe the appliance of the 6xFGB vector system for assessing leukemic cell traits in multiplex assays.
Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells.
Simplified inducible system for Trypanosoma brucei.
These days, most reverse genetics approaches in Trypanosoma brucei, a protozoan parasite of medical and veterinary significance, depend on pre-established cell strains. Consequently, inducible experimentation is diminished to some laboratory strains. Right here we described a brand new transgene expression system based mostly completely on endogenous transcription actions and a minimal set of regulatory elements that may simply been tailored to totally different strains. The pTbFIX vectors are designed to include the sequence of curiosity below the management of an inducible rRNA promoter together with a constitutive dicistronic unit encoding a nucleus focused tetracycline repressor and puromycin resistance genes in a tandem “head-to-tail” configuration. Upon doxycycline induction, the system helps regulatable GFP expression (170 to 400 fold) in each bloodstream and procyclic T. brucei kinds.
Moreover we’ve tailored the pTbFIX plasmid to carry out RNAi experimentation. Deadly phenotypes, together with α-tubulin and people akin to the enolase and clathrin heavy chain genes, had been efficiently recapitulated in procyclic and bloodstream parasites thus displaying the flexibility of this new device. we arrange CUG-expansion and Celf1 overexpression C2C12 cell fashions to mimic the myocyte differentiation defects of DM1, then transfected AdvmiR-206 into cell fashions, examined the extent of myogenic markers MyoD, MyoG, Mef2c, Celf1 by RT-PCRand Western Blotting, detected myotube formation by myosin heavy chain immunostaining. Consequence: 3′-UTR CUG-expansion results in myotube defects and impaired myoblasts differentiation.
 T&A Cloning Vector |
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| FYC002-20P | Yeastern Biotech | 1 vial | Ask for price |
 T&A Cloning Kits |
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| FYC001-20P | Yeastern Biotech | 1 vial | Ask for price |
 T-Pro Stacking Buffer |
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| JB03-C002 | T-Pro Biotechnology | 500ml/BT | EUR 152 |
 T-Pro MycoClean spray |
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| JT90-R002 | T-Pro Biotechnology | 500ml/BT | EUR 144 |
 T-Pro EZ Gel Solution 8% |
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| JB02-B008M | T-Pro Biotechnology | 500ml/BT | EUR 222 |
T-Pro EZ Gel Solution 8% |
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| JB02-B008S | T-Pro Biotechnology | 100ml/BT | EUR 135 |
 T-Pro EZ Gel Solution 10% |
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| JB02-B010M | T-Pro Biotechnology | 500ml/BT | EUR 222 |
T-Pro EZ Gel Solution 10% |
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| JB02-B010S | T-Pro Biotechnology | 100ml/BT | EUR 135 |
 T-Pro EZ Gel Solution 12% |
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| JB02-B012M | T-Pro Biotechnology | 500ml/BT | EUR 222 |
T-Pro EZ Gel Solution 12% |
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| JB02-B012S | T-Pro Biotechnology | 100ml/BT | EUR 135 |
 T-Pro EZ Gel Solution 15% |
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| JB02-B015M | T-Pro Biotechnology | 500ml/BT | EUR 222 |
T-Pro EZ Gel Solution 15% |
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| JB02-B015S | T-Pro Biotechnology | 100ml/BT | EUR 135 |
 T-Pro Separating or Resolving Buffer |
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| JB03-C001 | T-Pro Biotechnology | 500ml/BT | EUR 152 |
 T-Pro BCA Protein Assay kit |
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| JB04-D001 | T-Pro Biotechnology | 500assay/KIT | EUR 204 |
) T-Pro Transfer Blotting buffer (10X) |
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| JB08-H001 | T-Pro Biotechnology | 500ml/BT | EUR 135 |
 T-Pro Western Blot Stripping Reagent |
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| JB11-K002 | T-Pro Biotechnology | 500ml/BT | EUR 178 |
) T-Pro Aqua EZ Clean (M) |
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| JT90-R001M | T-Pro Biotechnology | 500ml/BT | EUR 332 |
) T-Pro Aqua EZ Clean (S) |
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| JT90-R001S | T-Pro Biotechnology | 100ml/BT | EUR 151 |
 T-Pro EZ stain Gel solution |
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| JT90-R005M | T-Pro Biotechnology | 500ml/BT | EUR 187 |
T-Pro EZ stain Gel solution |
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| JT90-R005S | T-Pro Biotechnology | 100ml/BT | EUR 126 |
 T-Pro P-Fect Transfection Reagent |
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| JT97-N005M | T-Pro Biotechnology | 1.0ml/vial | EUR 222 |
) T-Pro Plasmid Mini Kit (100) |
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| RB94-YPD100 | T-Pro Biotechnology | 100preps/Kit | EUR 161 |
) T-Pro Plasmid Mini Kit (250) |
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| RB94-YPD250 | T-Pro Biotechnology | 250preps/Kit | EUR 222 |
) T-Pro Plasmid Midi Kit (20) |
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| RB94-YPI020 | T-Pro Biotechnology | 20preps/Kit | EUR 213 |
) T-Pro Plasmid Maxi Kit (10) |
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| RB94-YPM010 | T-Pro Biotechnology | 10preps/Kit | EUR 222 |
 ELISA Kit) Testosterone (T) ELISA Kit |
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| DLR-T-Ge-48T | DL Develop | 48T | EUR 469 |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Testosterone (T) in samples from serum, plasma, urine or other biological fluids. |
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Testosterone (T) ELISA Kit |
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| DLR-T-Ge-96T | DL Develop | 96T | EUR 608 |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Testosterone (T) in samples from serum, plasma, urine or other biological fluids. |
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 T-Pro BCA Protein Assay Reagent A |
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| JB04-D001A | T-Pro Biotechnology | 500ml/BT | EUR 170 |
 T-Pro BCA Protein Assay Reagent B |
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| JB04-D001B | T-Pro Biotechnology | 12ml/BT | EUR 117 |
) T-Pro Bradford Protein Assay kit(1X) |
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| JB04-D002 | T-Pro Biotechnology | 500ml/BT | EUR 161 |
) T-Pro Fast Blocking Buffer (in PBS) |
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| JK92-W001 | T-Pro Biotechnology | 500ml/BT | EUR 187 |
) T-Pro Fast Blocking Buffer (in TBS) |
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| JK92-W002 | T-Pro Biotechnology | 500ml/BT | EUR 187 |
) T-Pro Genomic DNA Midi Kit (20) |
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| RB94-NGM020 | T-Pro Biotechnology | 20preps/kit | EUR 222 |
) T-Pro Genomic DNA Mini Kit (100) |
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| RB94-NGS100 | T-Pro Biotechnology | 100preps/kit | EUR 213 |
 ELISA Kit) General Testosterone (T) ELISA Kit |
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| RD-T-Ge-48Tests | Reddot Biotech | 48 Tests | EUR 467 |
General Testosterone (T) ELISA Kit |
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| RD-T-Ge-96Tests | Reddot Biotech | 96 Tests | EUR 646 |
General Testosterone (T) ELISA Kit |
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| RDR-T-Ge-48Tests | Reddot Biotech | 48 Tests | EUR 488 |
General Testosterone (T) ELISA Kit |
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| RDR-T-Ge-96Tests | Reddot Biotech | 96 Tests | EUR 676 |
 Reagent (reducing 4X)) T-Pro Laemmli (SDS sample) Reagent (reducing 4X) |
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| JB06-F002 | T-Pro Biotechnology | 10ml/BT | EUR 126 |
) T-Pro Tris-Glycine-SDS running buffer (10X) |
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| JB07-G001 | T-Pro Biotechnology | 500ml/BT | EUR 135 |
) T-Pro Tris-Glycine-Native running buffer (10X) |
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| JB07-G002 | T-Pro Biotechnology | 500ml/BT | EUR 135 |
) T-Pro Semi Dry Transfer Blotting buffer (10X) |
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| JB08-H002 | T-Pro Biotechnology | 500ml/BT | EUR 144 |
) T-Pro Protein Free Blocking Buffer (in PBS) |
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| JK92-W003 | T-Pro Biotechnology | 500ml/BT | EUR 187 |
) T-Pro Protein Free Blocking Buffer (in TBS) |
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| JK92-W004 | T-Pro Biotechnology | 500ml/BT | EUR 187 |
) T-Pro Nonliposomal Transfection Reagent I (NTR I) |
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| JT97-N001M | T-Pro Biotechnology | 1.0ml/vial | EUR 187 |
) T-Pro Nonliposomal Transfection Reagent II (NTR II) |
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| JT97-N002M | T-Pro Biotechnology | 1.0ml/vial | EUR 204 |
) T-Pro Nonliposomal Transfection Reagent III (NTR III) |
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| JT97-N006M | T-Pro Biotechnology | 1.0ml/vial | EUR 222 |
) T-Pro Endotoxin Removal Plasmid Midi kit (25) |
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| RB94-EPI020 | T-Pro Biotechnology | 20preps/Kit | EUR 248 |
) T-Pro Endotoxin Removal Plasmid Maxi kit (10) |
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| RB94-EPM010 | T-Pro Biotechnology | 10preps/Kit | EUR 265 |
) T-Pro Plant Genomic DNA Midi Kit (20) |
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| RB94-PGM020 | T-Pro Biotechnology | 20preps/kit | EUR 222 |
) T-Pro Plant Genomic DNA Mini Kit (100) |
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| RB94-PGS100 | T-Pro Biotechnology | 100preps/kit | EUR 222 |
 T&A Cloning Kit + ECOS 101DH5a |
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| FYC005-20P | Yeastern Biotech | 1 vial | Ask for price |
 Reagent (non-reducing 4X)) T-Pro Laemmli (SDS sample) Reagent (non-reducing 4X) |
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| JB06-F003 | T-Pro Biotechnology | 10ml/BT | EUR 126 |
) T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit) |
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| JT96-K002M | T-Pro Biotechnology | 250ml*2/Kit | EUR 204 |
T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit) |
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| JT96-K002S | T-Pro Biotechnology | 100ml*2/Kit | EUR 152 |
) T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit) |
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| JT96-K004M | T-Pro Biotechnology | 250ml*2/Kit | EUR 239 |
T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit) |
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| JT96-K004S | T-Pro Biotechnology | 100ml*2/Kit | EUR 170 |
) T-Pro Gel/PCR DNA Purification Maxi Kit (10) |
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| RB94-NEL010 | T-Pro Biotechnology | 10preps/Kit | EUR 161 |
) T-Pro Gel/PCR DNA Purification Mini Kit (100) |
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| RB94-NES100 | T-Pro Biotechnology | 100preps/Kit | EUR 161 |
) T-Pro Gel/PCR DNA Purification Mini Kit (250) |
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| RB94-NES250 | T-Pro Biotechnology | 250preps/Kit | EUR 222 |
) pET His6 Sumo TEV LIC cloning vector (2S-T) |
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| PVT14035 | Lifescience Market | 2 ug | EUR 599 |
) T-Pro Washing buffer in PBS and Tween-20 (10X) |
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| JB09-I003 | T-Pro Biotechnology | 500ml/BT | EUR 144 |
) T-Pro Washing buffer in TBS and Tween-20 (10X) |
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| JB09-I004 | T-Pro Biotechnology | 500ml/BT | EUR 144 |
 for western blot washing) T-Pro Phosphate-Buffered Saline (PBS, 10X) for western blot washing |
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| JB09-I001 | T-Pro Biotechnology | 500ml/BT | EUR 135 |
 for western blot washing) T-Pro Tris-Buffered Saline (TBS, 10X) for western blot washing |
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| JB09-I002 | T-Pro Biotechnology | 500ml/BT | EUR 135 |
 T&A Cloning Kit + ECOS 9-5JM109 |
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| FYC006-20P | Yeastern Biotech | 1 vial | Ask for price |
 Vector T Plasmid |
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| PVT7016 | Lifescience Market | 2 ug | EUR 262 |
) siRNA Cloning Vector (pGB) |
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| 9500-20 | Biovision | EUR 294 | |
 (pORF)) T ORF Vector (Rat) (pORF) |
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| ORF077342 | ABM | 1.0 ug DNA | EUR 506 |
 (pORF)) T ORF Vector (Human) (pORF) |
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| ORF033613 | ABM | 1.0 ug DNA | EUR 405 |
 (pORF)) T ORF Vector (Mouse) (pORF) |
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| ORF058986 | ABM | 1.0 ug DNA | EUR 506 |
 dCas9 C-terminal Cloning Vector |
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| K014 | ABM | 10 ug | EUR 721 |
 Immortalized Hamster Hepatocytes-SV40 T+t |
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| T0071 | ABM | 1x106 cells / 1.0 ml | Ask for price |
 Immortalized Monkey Hepatocytes-SV40 T+t |
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| T0074 | ABM | 1x106 cells / 1.0 ml | Ask for price |
 polyclonal antibody) Transcription factor T (T) polyclonal antibody |
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| ABP-PAB-11172 | Allele Biotech | 100 ug | Ask for price |
Transcription factor T (T) polyclonal antibody |
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| ABP-PAB-11173 | Allele Biotech | 100 ug | Ask for price |
 T-5224 |
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| 9546-25 | Biovision | EUR 686 | |
T-5224 |
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| 9546-5 | Biovision | EUR 218 | |
 T Antibody |
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| BF0015 | Affbiotech | 200ul | EUR 376 |
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Description: T antibody detects endogenous levels of total T. |
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 Esculentoside T |
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| TB0765-0050 | ChemNorm | 10mg | EUR 271 |
 Gomisin T |
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| TBZ0382 | ChemNorm | unit | Ask for price |
 Peptide T |
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| 5-01748 | CHI Scientific | 4 x 5mg | Ask for price |
 DMT-T |
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| 5-02235 | CHI Scientific | 5g | Ask for price |
DMT-T |
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| 5-02236 | CHI Scientific | 25g | Ask for price |
 T siRNA |
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| 20-abx938300 | Abbexa |
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T siRNA |
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| 20-abx938301 | Abbexa |
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 Thioflavine T |
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| 20-abx184437 | Abbexa |
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 T-00127_HEV1 |
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| HY-108313 | MedChemExpress | 1mg | EUR 187 |
 T-448 |
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| HY-122635A | MedChemExpress | 1mg | EUR 681 |
T-5224 |
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| HY-12270 | MedChemExpress | 100mg | EUR 1069 |
 T-26c |
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| HY-100518 | MedChemExpress | 10mM/1mL | EUR 201 |
 T-1105 |
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| HY-W015764 | MedChemExpress | 10mM/1mL | EUR 113 |
 T 82 |
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| HY-U00028 | MedChemExpress | 5mg | EUR 2846 |
Peptide T |
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| HY-P0272 | MedChemExpress | 5mg | EUR 302 |
 Chloramine-T |
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| HY-B0959 | MedChemExpress | 1g | EUR 119 |
 Thioflavin T |
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| HY-D0218 | MedChemExpress | 10mM/1mL | EUR 113 |
 T. gondii |
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| DAG1494 | Creative Diagnostics | 100 µg | EUR 645 |
T. gondii |
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| DAG1495 | Creative Diagnostics | 100 µg | EUR 645 |
T. gondii |
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| DAG1496 | Creative Diagnostics | 100 µg | EUR 645 |
T Antibody |
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| 48451-100ul | SAB | 100ul | EUR 333 |
T Antibody |
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| 48451-50ul | SAB | 50ul | EUR 239 |
T-5224 |
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| B4664-10 | ApexBio | 10 mg | EUR 224 |
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Description: T-5224 is a non-peptidic, small molecule and novel inhibitor of c-Fos/AP-1 [1].T-5224 has shown the specific inhibition in a c-Fos/AP-1promoter-luciferase assay, without affecting the levels of c-Fos family protein members themselves. |
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T-5224 |
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| B4664-100 | ApexBio | 100 mg | EUR 920 |
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Description: T-5224 is a non-peptidic, small molecule and novel inhibitor of c-Fos/AP-1 [1].T-5224 has shown the specific inhibition in a c-Fos/AP-1promoter-luciferase assay, without affecting the levels of c-Fos family protein members themselves. |
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T-5224 |
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| B4664-5 | ApexBio | 5 mg | EUR 166 |
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Description: T-5224 is a non-peptidic, small molecule and novel inhibitor of c-Fos/AP-1 [1].T-5224 has shown the specific inhibition in a c-Fos/AP-1promoter-luciferase assay, without affecting the levels of c-Fos family protein members themselves. |
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T-5224 |
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| B4664-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 177 |
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Description: T-5224 is a non-peptidic, small molecule and novel inhibitor of c-Fos/AP-1 [1].T-5224 has shown the specific inhibition in a c-Fos/AP-1promoter-luciferase assay, without affecting the levels of c-Fos family protein members themselves. |
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T-5224 |
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| B4664-50 | ApexBio | 50 mg | EUR 572 |
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Description: T-5224 is a non-peptidic, small molecule and novel inhibitor of c-Fos/AP-1 [1].T-5224 has shown the specific inhibition in a c-Fos/AP-1promoter-luciferase assay, without affecting the levels of c-Fos family protein members themselves. |
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 T 5601640 |
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| B4940-10 | ApexBio | 10 mg | EUR 239 |
T 5601640 |
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| B4940-100 | ApexBio | 100 mg | EUR 1281 |
T 5601640 |
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| B4940-25 | ApexBio | 25 mg | EUR 448 |
T 5601640 |
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| B4940-5 | ApexBio | 5 mg | EUR 158 |
T 5601640 |
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| B4940-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 168 |
T 5601640 |
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| B4940-50 | ApexBio | 50 mg | EUR 756 |
 Conantokin-T |
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| B5378-.5 | ApexBio | 500 ug | EUR 561 |
 T 705 |
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| B1114-25 | ApexBio | 25 mg | EUR 374 |
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Description: T 705(Favipiravir) is a RNA-directed RNA polymerase NS5B inhibitor. |
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T 705 |
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| B1114-5 | ApexBio | 5 mg | EUR 137 |
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Description: T 705(Favipiravir) is a RNA-directed RNA polymerase NS5B inhibitor. |
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T 705 |
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| B1114-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 108 |
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Description: T 705(Favipiravir) is a RNA-directed RNA polymerase NS5B inhibitor. |
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 T 98475 |
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| B7090-1 | ApexBio | 1 mg | EUR 438 |
 T-Peptide |
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| H-8126.0500 | Bachem | 0.5mg | EUR 294 |
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Description: Sum Formula: C92H171N45O18; CAS# [2022956-62-1] net |
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T-Peptide |
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| H-8126.1000 | Bachem | 1.0mg | EUR 477 |
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Description: Sum Formula: C92H171N45O18; CAS# [2022956-62-1] net |
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 RapidSeq High Yield Directional mRNA Sample Prep Kit, Trial version enough for 2 rxn. Customer select aligners |
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| KS073012-T | Biochain | 1 Kit | EUR 291 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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 RapidSeq High Yield Small RNA Sample Prep Kit, Trial version enough for 2 rxn. Customer select aligners |
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| KS074012-T | Biochain | 1 Kit | EUR 291 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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) DIRECTPCR LYSIS REAGENT (TAIL) |
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| 101-T | Viagen | 50 ml | EUR 141 |
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Description: Up to 250 tails (50ml) |
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DIRECTPCR LYSIS REAGENT (TAIL) |
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| 102-T | Viagen | 100ml | EUR 207 |
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Description: Up to 500 tails (50ml) |
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) TrueBlack IF Background Suppressor System (Permeabilizing) |
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| 23012-T | Biotium | 20AS | EUR 93 |
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Description: Minimum order quantity: 1 unit of 20AS |
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 TrueBlack WB Blocking Buffer Kit |
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| 23013-T | Biotium | 1kit | EUR 125 |
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Description: Minimum order quantity: 1 unit of 1kit |
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 WarmStart Enzyme Modification Kit, 0.5 mg Reaction |
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| 29053-T | Biotium | 0.5mg | EUR 266 |
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Description: Minimum order quantity: 1 unit of 0.5mg |
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 HotStart Enzyme Modification Kit, 0.5 mg Reaction |
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| 29054-T | Biotium | 0.5mg | EUR 266 |
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Description: Minimum order quantity: 1 unit of 0.5mg |
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) JC-1 Mitochondrial Membrane Potential Detection Kit (25 assays) |
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| 30001-T | Biotium | 1kit | EUR 127 |
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Description: Minimum order quantity: 1 unit of 1kit |
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) Viability/Cytotoxity Assay Kit for Animal Live & Dead Cell (150 assays) |
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| 30002-T | Biotium | 1KIT | EUR 183 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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) ATP-Glo Bioluminometric Cell Viability Assay Kit (50 assays) |
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| 30020-T | Biotium | 1KIT | EUR 85 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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(4 mL)) Steady-Luc Firefly HTS Assay Kit (trial size)(4 mL) |
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| 30028-T | Biotium | 1KIT | EUR 94 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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 NucView 488 CASPASE 3 ASSAY KIT, TRIAL SIZE |
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| 30029-T | Biotium | 1ST | EUR 152 |
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Description: Minimum order quantity: 1 unit of 1ST |
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 ViaFluor 405 SE Cell Proliferation Kit |
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| 30068-T | Biotium | 1KIT | EUR 121 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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 AccuOrangeâ„¢ Protein Quantitation Kit, 200 assays |
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| 30071-T | Biotium | 1KIT | EUR 101 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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 Firefly & Renilla Luciferase Single Tube Assay Kit |
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| 30081-T | Biotium | 50AS | EUR 155 |
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Description: Minimum order quantity: 1 unit of 50AS |
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 Renilla Luciferase Assay Kit 2.0 |
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| 30082-T | Biotium | 50AS | EUR 114 |
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Description: Minimum order quantity: 1 unit of 50AS |
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 Firefly Luciferase Assay Kit 2.0 |
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| 30085-T | Biotium | 50AS | EUR 109 |
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Description: Minimum order quantity: 1 unit of 50AS |
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) ViaFluor 488 SE Cell Proliferation Kit, Trial Size (1 vial, 100 assays) |
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| 30086-T | Biotium | 1KIT | EUR 104 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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 CellBrite Fix 555, Trial Size |
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| 30088-T | Biotium | 1KIT | EUR 195 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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 CellBrite Fix 640, Trial Size |
|||
| 30089-T | Biotium | 1KIT | EUR 195 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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 CellBriteâ„¢ Fix 488, Trial Size |
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| 30090-T | Biotium | 1KIT | EUR 222 |
|
Description: Minimum order quantity: 1 unit of 1KIT |
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Overexpression of Celf1 inhibits myoblast differentiation and impairs differentiation. Knockdown of Celf1 partially rescues differentiation defects of myoblasts harboring CUG-expansion. miR-206 incompletely reverses myoblast differentiation inhibition induced by CUG-expansion and partially recuses myoblast differentiation defects induced by Celf1 overexpression.