Enhanced snoMEN Vectors Facilitate Establishment

gfpvector

Enhanced snoMEN Vectors Facilitate Institution of GFP-HIF-1α Protein Substitute Human Cell Traces.

The snoMEN (snoRNA Modulator of gene ExpressioN) vector expertise was developed from a human field C/D snoRNA, HBII-180C, which accommodates an inside sequence that may be manipulated to make it complementary to RNA targets, permitting knock-down of focused genes. Right here we’ve got screened further human nucleolar snoRNAs and assessed their software for gene particular knock-downs to enhance the effectivity of snoMEN vectors.
We establish and characterise a brand new snoMEN vector, termed 47snoMEN, that’s derived from field C/D snoRNA U47, demonstrating its use for knock-down of each endogenous mobile proteins and G/YFP-fusion proteins.
Utilizing multiplex 47snoMEM vectors that co-express a number of 47snoMEN in a single transcript, every of which may goal totally different websites in the identical mRNA, we doc >3-fold improve in knock-down effectivity when put next with the unique HBII-180C primarily based snoMEN.
The multiplex 47snoMEM vector allowed the development of human protein alternative cell traces with improved effectivity, together with the institution of novel GFP-HIF-1α alternative cells.
Quantitative mass spectrometry evaluation confirmed the improved effectivity and specificity of protein alternative utilizing the 47snoMEN-PR vectors. The 47snoMEN vectors develop the potential purposes for snoMEN expertise in gene expression research, goal validation and gene remedy
gfpvector

gfpvector

NACC2 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV697085 1.0 ug DNA
EUR 818.4

NACC2 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV697086 1.0 ug DNA
EUR 818.4

NACC1 Protein Vector (Human) (pPM-C-HA)

PV103488 500 ng
EUR 662.4

NACC1 Protein Vector (Human) (pPB-C-His)

PV103486 500 ng
EUR 662.4

NACC1 Protein Vector (Human) (pPB-N-His)

PV103487 500 ng
EUR 662.4

NACC1 Protein Vector (Human) (pPM-C-His)

PV103489 500 ng
EUR 662.4

NACC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV697082 1.0 ug DNA
EUR 818.4

NACC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV697083 1.0 ug DNA
EUR 888

NACC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV697084 1.0 ug DNA
EUR 888

Nacc1 ORF Vector (Rat) (pORF)

ORF071065 1.0 ug DNA
EUR 607.2

Nacc1 ORF Vector (Mouse) (pORF)

ORF050990 1.0 ug DNA
EUR 607.2

NACC1 sgRNA CRISPR Lentivector set (Human)

K1386401 3 x 1.0 ug
EUR 406.8

Nacc1/ Rat Nacc1 ELISA Kit

ELI-37475r 96 Tests
EUR 1063.2

NACC1

E8EM1709-73 100ul
EUR 275
Description: Available in various conjugation types.

NACC1 sgRNA CRISPR Lentivector (Human) (Target 1)

K1386402 1.0 ug DNA
EUR 184.8

NACC1 sgRNA CRISPR Lentivector (Human) (Target 2)

K1386403 1.0 ug DNA
EUR 184.8

NACC1 sgRNA CRISPR Lentivector (Human) (Target 3)

K1386404 1.0 ug DNA
EUR 184.8

NACC1 Protein Vector (Rat) (pPM-C-HA)

PV284260 500 ng
EUR 723.6

Human NACC1 shRNA Plasmid

20-abx964300
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

NACC1 Protein Vector (Rat) (pPB-C-His)

PV284258 500 ng
EUR 723.6

NACC1 Protein Vector (Rat) (pPB-N-His)

PV284259 500 ng
EUR 723.6

NACC1 Protein Vector (Rat) (pPM-C-His)

PV284261 500 ng
EUR 723.6

NACC1 Protein Vector (Mouse) (pPM-C-HA)

PV203960 500 ng
EUR 723.6

NACC1 Protein Vector (Mouse) (pPB-C-His)

PV203958 500 ng
EUR 723.6

NACC1 Protein Vector (Mouse) (pPB-N-His)

PV203959 500 ng
EUR 723.6

NACC1 Protein Vector (Mouse) (pPM-C-His)

PV203961 500 ng
EUR 723.6

NACC1 Recombinant Protein (Human)

RP077613 100 ug Ask for price

NACC1 siRNA

20-abx903454
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

NACC1 siRNA

20-abx925337
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

NACC1 siRNA

20-abx925338
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

Human NACC1 Protein Lysate 20ug

IHUNACC1PLLY20UG each
EUR 213
Description: Human NACC1 Protein Lysate 20ug

NACC1 Antibody

BF0187 200ul
EUR 540

NACC1 Antibody

48470-100ul 100ul
EUR 399.6

NACC1 Antibody

48470-50ul 50ul
EUR 286.8

NACC1 Antibody

CSB-PA283286- each
EUR 402
Description: A polyclonal antibody against NACC1. Recognizes NACC1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

NACC1 Antibody

CSB-PA283286-100ul 100ul
EUR 379.2
Description: A polyclonal antibody against NACC1. Recognizes NACC1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

NACC1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K1386405 3 x 1.0 ug
EUR 451.2

Nacc1 sgRNA CRISPR Lentivector set (Rat)

K7051401 3 x 1.0 ug
EUR 406.8

Nacc1 sgRNA CRISPR Lentivector set (Mouse)

K4905601 3 x 1.0 ug
EUR 406.8

NACC1 Mouse mAb

E2220504 100ul
EUR 225
Description: Available in various conjugation types.

NACC1 Rabbit pAb

A11709-100ul 100 ul
EUR 369.6

NACC1 Rabbit pAb

A11709-200ul 200 ul
EUR 550.8

NACC1 Rabbit pAb

A11709-20ul 20 ul
EUR 219.6

NACC1 Rabbit pAb

A11709-50ul 50 ul
EUR 267.6

NACC1 Rabbit pAb

A11720-100ul 100 ul
EUR 369.6

NACC1 Rabbit pAb

A11720-200ul 200 ul
EUR 550.8

NACC1 Rabbit pAb

A11720-20ul 20 ul
EUR 219.6

NACC1 Rabbit pAb

A11720-50ul 50 ul
EUR 267.6

NACC1 Rabbit pAb

E2511720 100ul
EUR 225
Description: Available in various conjugation types.

NACC1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K1386406 1.0 ug DNA
EUR 200.4

NACC1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K1386407 1.0 ug DNA
EUR 200.4

NACC1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K1386408 1.0 ug DNA
EUR 200.4

NACC1 Blocking Peptide

BF0187-BP 1mg
EUR 234

Nacc1 sgRNA CRISPR Lentivector (Rat) (Target 1)

K7051402 1.0 ug DNA
EUR 184.8

Nacc1 sgRNA CRISPR Lentivector (Rat) (Target 2)

K7051403 1.0 ug DNA
EUR 184.8

Nacc1 sgRNA CRISPR Lentivector (Rat) (Target 3)

K7051404 1.0 ug DNA
EUR 184.8

NACC1 Conjugated Antibody

C48470 100ul
EUR 476.4

NACC1 Polyclonal Antibody

E911709 100ul
EUR 225
Description: Available in various conjugation types.

NACC1 Polyclonal Antibody

E911720 100ul
EUR 225
Description: Available in various conjugation types.

NACC1 Monoclonal Antibody

E10-30137a 100μg/100μl
EUR 225
Description: Available in various conjugation types.

Nacc1 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4905602 1.0 ug DNA
EUR 184.8

Nacc1 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4905603 1.0 ug DNA
EUR 184.8

Nacc1 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4905604 1.0 ug DNA
EUR 184.8

Rat NACC1 shRNA Plasmid

20-abx987779
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

NACC1 (Center) Rabbit pAb

E2617985 100ul
EUR 225
Description: Available in various conjugation types.

NACC1 Antibody / Nac1 / BTBD14B

V9183-100UG 100ug
EUR 349.3
Description: The BTB (broad-complex, tramtrack and bric a brac) domain, also known as the POZ (poxvirus and zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. BTBD14B (BTB/POZ domain-containing protein 14B), also known as NACC1 (nucleus accumbens associated 1), BEND8 or NAC1, is a 527 amino acid protein that localizes to both the nucleus and the cytoplasm and contains one BTB (POZ) domain. Existing as a homo-oligomer that interacts with HDAC3 and HDAC4, BTBD14B functions as a transcriptional repressor that influences the transcriptional activity of CRIF1 and is required for proteasome recruitment to the nucleus and cytoplasm in dendritic spines. BTBD14B is overexpressed in multiple carcinomas, suggesting a role in tumor development and metastasis.

NACC1 Antibody / Nac1 / BTBD14B

V9183-20UG 20ug
EUR 153.3
Description: The BTB (broad-complex, tramtrack and bric a brac) domain, also known as the POZ (poxvirus and zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. BTBD14B (BTB/POZ domain-containing protein 14B), also known as NACC1 (nucleus accumbens associated 1), BEND8 or NAC1, is a 527 amino acid protein that localizes to both the nucleus and the cytoplasm and contains one BTB (POZ) domain. Existing as a homo-oligomer that interacts with HDAC3 and HDAC4, BTBD14B functions as a transcriptional repressor that influences the transcriptional activity of CRIF1 and is required for proteasome recruitment to the nucleus and cytoplasm in dendritic spines. BTBD14B is overexpressed in multiple carcinomas, suggesting a role in tumor development and metastasis.

NACC1 Antibody / Nac1 / BTBD14B

V9183SAF-100UG 100ug
EUR 349.3
Description: The BTB (broad-complex, tramtrack and bric a brac) domain, also known as the POZ (poxvirus and zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. BTBD14B (BTB/POZ domain-containing protein 14B), also known as NACC1 (nucleus accumbens associated 1), BEND8 or NAC1, is a 527 amino acid protein that localizes to both the nucleus and the cytoplasm and contains one BTB (POZ) domain. Existing as a homo-oligomer that interacts with HDAC3 and HDAC4, BTBD14B functions as a transcriptional repressor that influences the transcriptional activity of CRIF1 and is required for proteasome recruitment to the nucleus and cytoplasm in dendritic spines. BTBD14B is overexpressed in multiple carcinomas, suggesting a role in tumor development and metastasis.

NACC1 Antibody / NAC1 / BTBD14B

RQ6781 100ug
EUR 356.15
Description: Nucleus accumbens-associated protein 1 is a protein that in humans is encoded by the NACC1 gene. This gene encodes a member of the BTB/POZ protein family. BTB/POZ proteins are involved in several cellular processes including proliferation, apoptosis and transcription regulation. The encoded protein is a transcriptional repressor that plays a role in stem cell self-renewal and pluripotency maintenance. The encoded protein also suppresses transcription of the candidate tumor suppressor Gadd45GIP1, and expression of this gene may play a role in the progression of multiple types of cancer. A pseudogene of this gene is located on the short arm of chromosome 9.

Mouse NACC1 shRNA Plasmid

20-abx975804
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

NACC1 Recombinant Protein (Rat)

RP213191 100 ug Ask for price

NACC1 Recombinant Protein (Mouse)

RP152966 100 ug Ask for price

Polyclonal NACC1 Antibody (Center)

APR04758G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human NACC1 (Center). This antibody is tested and proven to work in the following applications:

NACC1 (C-term) Rabbit pAb

E2618067 100ul
EUR 225
Description: Available in various conjugation types.

Nacc1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K7051405 3 x 1.0 ug
EUR 451.2

Nacc1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K4905605 3 x 1.0 ug
EUR 451.2

NACC2 ORF Vector (Human) (pORF)

ORF025873 1.0 ug DNA
EUR 486

Polyclonal NACC1 Antibody (C-term)

APR04778G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human NACC1 (C-term). This antibody is tested and proven to work in the following applications:

Human Nucleus accumbens- associated protein 1, NACC1 ELISA KIT

ELI-16141h 96 Tests
EUR 988.8

NACA3P Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV771609 1.0 ug DNA
EUR 1173.6

A replicating plasmid-based vector for GFP expression in Mycoplasma hyopneumoniae.

Mycoplasma hyopneumoniae (M. hyopneumoniae) causes porcine enzootic pneumonia (PEP) that considerably impacts the pig trade worldwide. Regardless of the provision of the entire genome sequence, research on the pathogenesis of this organism have been restricted as a result of lack of a genetic manipulation system.
Subsequently, the intention of the present examine was to generate a common GFP reporter vector primarily based on a replicating plasmid. Right here, we describe the feasibility of GFP reporter expression in M. hyopneumoniae (pressure 168L) managed by the p97 gene promoter of this mycoplasma. An expression plasmid (pMD18-TOgfp) containing the p97 gene promoter, and origin of replication (oriC) of M. hyopneumoniae, tetracycline resistant marker (tetM), and GFP was constructed and used to rework competent M. hyopneumoniae cells.
We noticed inexperienced fluorescence in M. hyopneumoniae transformants beneath fluorescence microscopy, which signifies that there was expression of the GFP reporter that was pushed by the p97 gene promoter.
Moreover, an electroporation methodology for M. hyopneumoniae with an effectivity of roughly 1 x 10(-6) transformants/μg plasmid DNA was optimized and is described herein. In conclusion, our information display the susceptibility of M. hyopneumoniae to genetic manipulation whereby overseas genes are expressed. This work might encourage the event of genetic instruments to govern the genome of M. hyopneumoniae for practical genomic analyses.

Human adipose derived mesenchymal stromal cells transduced with GFP lentiviral vectors: evaluation of immunophenotype and differentiation capability in vitro.

Adipose derived mesenchymal stromal/stem cells (ASCs) are a heterogeneous inhabitants characterised by (a) their potential to stick to plastic; (b) immunophenotypic expression of sure cell floor markers, whereas missing others; and (c) the capability to distinguish into lineages of mesodermal origin together with osteocytes, chondrocytes and adipocytes. The long-term objective is to make the most of these cells for medical translation into cell-based therapies. Nonetheless, preclinical security and efficacy have to be demonstrated in animal fashions.
ASCs will also be utilized as organic autos for vector-based gene supply techniques, since they’re believed to dwelling to websites of irritation and an infection in vivo. These elements motivated the event of a labelling system for ASCs utilizing lentiviral vector-based inexperienced fluorescent protein (GFP) transduction. Human ASCs had been transduced with GFP-expressing lentiviral vectors. A titration examine decided the viral titer required to transduce the utmost variety of ASCs.
The impact of the transduced GFP lentiviral vector on ASC immunophenotypic expression of floor markers in addition to their potential to distinguish into osteocytes and adipocytes had been assessed in vitro.
Transduction effectivity in ASC cultures of roughly 80 % was noticed with an MOI of ~118. No important immunophenotypic variations had been noticed between transduced and non-transduced cells and each cell sorts efficiently differentiated into adipocytes and osteocytes in vitro. We obtained >80 % transduction of ASCs utilizing GFP lentiviral vectors.
Transduced ASCs maintained plastic adherence, demonstrated ASC immunophenotype and the flexibility to distinguish into cells of the mesodermal lineage. This GFP-ASC transduction approach provides a possible monitoring system for future pre-clinical research.

100G Acrylamide

NAT1014 100G
EUR 120

500G Acrylamide

NAT1016 500G
EUR 261.6

1KG Acrylamide

NAT1018 1KG
EUR 412.8

25G AquaPor LE

NAT1020 25G
EUR 132

100G AquaPor LE GTAC

NAT1022 100G
EUR 250.8

500G AquaPor LE GTAC

NAT1024 500G
EUR 878.4

25G AquaPor ES

NAT1026 25G
EUR 152.4

100G AquaPor ES

NAT1028 100G
EUR 350.4

25G AquaPor LM

NAT1030 25G
EUR 288

100G AquaPor LM

NAT1032 100G
EUR 860.4

25G AquaPor HR

NAT1034 25G
EUR 236.4

100G AquaPor HR

NAT1036 100G
EUR 592.8

25G AquaPor 3:1

NAT1038 25G
EUR 250.8

100G AquaPor 3:1

NAT1040 100G
EUR 570

25G Bis (Methylene Bis-Acrylamide)

NAT1042 25G
EUR 100.8

25G DATD Ultrapure

NAT1044 25G
EUR 168

250G Glycine

NAT1046 250G
EUR 94.8

1KG Glycine Ultrapure

NAT1048 1KG
EUR 128.4

250G Tris

NAT1050 250G
EUR 102

1KG Tris Ultrapure

NAT1052 1KG
EUR 177.6

100G Tricine

NAT1054 100G
EUR 120

100G Ion Exchange Resin (mixed bed)

NAT1056 100G
EUR 96

25G Riboflavin

NAT1058 25G
EUR 96

25ML TEMED

NAT1060 25ML
EUR 99.6

25G Ammonium Persulfate

NAT1062 25G
EUR 96

100G Ammonium Persulfate

NAT1064 100G
EUR 118.8

1G Dithiothreitol (DTT)

NAT1066 1G
EUR 103.2

5G Dithiothreitol (DTT)

NAT1068 5G
EUR 183.6

50ML 2-Mercaptoethanol

NAT1070 50ML
EUR 112.8

100G SDS Ultrapure

NAT1072 100G
EUR 111.6

1KG SDS Ultrapure

NAT1074 1KG
EUR 457.2

250G Urea

NAT1076 250G
EUR 98.4

1KG Urea

NAT1078 1KG
EUR 138

450ML Glycerol Ultrapure

NAT1080 450ML
EUR 132

200ML Tween 20 Ultrapure

NAT1082 200ML
EUR 132

1L Tween 20

NAT1084 1L
EUR 169.2

200ML Formamide

NAT1086 200ML
EUR 111.6

500ML Boric Acid

NAT1088 500ML
EUR 103.2

100G EDTA

NAT1090 100G
EUR 94.8

500G EDTA

NAT1092 500G
EUR 177.6

GelDry Film 11 X 12 cm

NAT1094 PK50
EUR 100.8

25ML Formamide CE

NAT1096 25ML Ask for price

25ML Glass Bond

NAT1098 25ML
EUR 158.4

450ML Glass Free

NAT1100 450ML
EUR 196.8

GelDry Film 22.5 X 22.5 cm

NAT1102 PK50
EUR 130.8

1L DEPC Treated Water

NAT1104 1L
EUR 120

Sterling Rapid Silver Stain Kit

NAT1106 1KIT
EUR 265.2

1L ProtoBlue Safe

NAT1108 1L
EUR 159.6

4L ProtoBlue Safe

NAT1110 4L
EUR 282

1L ProtoStain Blue

NAT1112 1L
EUR 164.4

25ML Nuclistain

NAT1114 25ML
EUR 128.4

450ML AcrylaGel 30%

NAT1116 450ML
EUR 128.4

1L AcrylaGel 30%

NAT1118 1L
EUR 177.6

450ML Bis-AcrylaGel 2%

NAT1120 450ML
EUR 98.4

1L Bis-AcrylaGel 2%

NAT1122 1L
EUR 111.6

450ML UreaGel 29:1 Concentrate

NAT1124 450ML
EUR 133.2

1L UreaGel 29:1 Concentrate

NAT1126 1L
EUR 183.6

UreaGel 29:1 System 1L Kit

NAT1128 EACH
EUR 171.6

UreaGel 29:1 System 2.2L Kit

NAT1130 EACH
EUR 253.2

450ML SequaGel UreaGel Concentrate

NAT1132 450ML
EUR 126

1L SequaGel UreaGel Concentrate

NAT1134 1L
EUR 160.8

SequaGel Sequencing System 1L Kit

NAT1136 1KIT
EUR 171.6

SequaGel Sequencing System 2.2L Kit

NAT1138 EACH
EUR 253.2

100ML SequaGel Buffer

NAT1140 100ML
EUR 80.4

200ML SequaGel UreaGel Buffer

NAT1142 200ML
EUR 84

450ML SequaGel UreaGel-6

NAT1144 450ML
EUR 115.2

1L SequaGel UreaGel-6

NAT1146 1L
EUR 151.2

450ML SequaGel UreaGel-8

NAT1148 450ML
EUR 115.2

1L SequaGel UreaGel-8

NAT1150 1L
EUR 151.2

450ML SequaGel UreaGel Diluent

NAT1152 450ML
EUR 90

1L SequaGel UreaGel Diluent

NAT1154 1L
EUR 103.2

90ML SequaGel Complete Buffer

NAT1156 90ML
EUR 79.2

200ML SequaGel UreaGel Complete Buffer

NAT1158 200ML
EUR 85.2

450ML SequaGel XR

NAT1160 450ML
EUR 126

1L SequaGel XR

NAT1162 1L
EUR 165.6

100ML SequaGel XR Concentrate

NAT1164 100ML
EUR 128.4

450ML SequaGel XR Concentrate

NAT1166 450ML
EUR 361.2

200ML SequaGel MD Monomer Solution

NAT1168 200ML
EUR 220.8

SequaGel MD SSCP Kit

NAT1170 1KIT
EUR 253.2

SequaGel MD Heteroduplex Kit

NAT1172 EACH
EUR 265.2

1.2ML SequaGel MD SSCP Stop Solution

NAT1174 1.2ML
EUR 85.2

450ML AccuGel 19:1 (30%)

NAT1176 450ML
EUR 126

1L AccuGel 19:1 (30%)

NAT1178 1L
EUR 165.6

450ML AccuGel 19:1 (40%)

NAT1180 450ML
EUR 130.8

1L AccuGel 19:1 (40%)

NAT1182 1L
EUR 168

450ML AccuGel 29:1 (30%)

NAT1184 450ML
EUR 126

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