Enhanced snoMEN Vectors Facilitate Establishment

gfpvector

Enhanced snoMEN Vectors Facilitate Institution of GFP-HIF-1α Protein Substitute Human Cell Traces.

The snoMEN (snoRNA Modulator of gene ExpressioN) vector expertise was developed from a human field C/D snoRNA, HBII-180C, which accommodates an inside sequence that may be manipulated to make it complementary to RNA targets, permitting knock-down of focused genes. Right here we’ve got screened further human nucleolar snoRNAs and assessed their software for gene particular knock-downs to enhance the effectivity of snoMEN vectors.
We establish and characterise a brand new snoMEN vector, termed 47snoMEN, that’s derived from field C/D snoRNA U47, demonstrating its use for knock-down of each endogenous mobile proteins and G/YFP-fusion proteins.
Utilizing multiplex 47snoMEM vectors that co-express a number of 47snoMEN in a single transcript, every of which may goal totally different websites in the identical mRNA, we doc >3-fold improve in knock-down effectivity when put next with the unique HBII-180C primarily based snoMEN.
The multiplex 47snoMEM vector allowed the development of human protein alternative cell traces with improved effectivity, together with the institution of novel GFP-HIF-1α alternative cells.
Quantitative mass spectrometry evaluation confirmed the improved effectivity and specificity of protein alternative utilizing the 47snoMEN-PR vectors. The 47snoMEN vectors develop the potential purposes for snoMEN expertise in gene expression research, goal validation and gene remedy
gfpvector

gfpvector

NACC1 Antibody

CSB-PA283286-
EUR 335
  • Form: liquid
  • Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibo
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Description: A polyclonal antibody against NACC1. Recognizes NACC1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

NACC1 Antibody

CSB-PA283286-100ul 100ul
EUR 316
  • Form: liquid
  • Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibo
  • Show more
Description: A polyclonal antibody against NACC1. Recognizes NACC1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

NACC1 Antibody

BF0187 200ul
EUR 376
Description: NACC1 antibody detects endogenous levels of total NACC1.

NACC1 siRNA

20-abx903454
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

NACC1 siRNA

20-abx925337
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

NACC1 siRNA

20-abx925338
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

Human NACC1 shRNA Plasmid

20-abx964300
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

NACC1 Recombinant Protein (Human)

RP077613 100 ug Ask for price

NACC1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV693127 1.0 ug DNA
EUR 682

NACC1 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV693131 1.0 ug DNA
EUR 682

NACC1 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV693132 1.0 ug DNA
EUR 682

Nacc1 ORF Vector (Rat) (pORF)

ORF071065 1.0 ug DNA
EUR 506

Nacc1 ORF Vector (Mouse) (pORF)

ORF050990 1.0 ug DNA
EUR 506

NACC1 Rabbit pAb

A11709-100ul 100 ul
EUR 308

NACC1 Rabbit pAb

A11709-200ul 200 ul
EUR 459

NACC1 Rabbit pAb

A11709-20ul 20 ul
EUR 183

NACC1 Rabbit pAb

A11709-50ul 50 ul
EUR 223

NACC1 Rabbit pAb

A11720-100ul 100 ul
EUR 308

NACC1 Rabbit pAb

A11720-200ul 200 ul
EUR 459

NACC1 Rabbit pAb

A11720-20ul 20 ul
EUR 183

NACC1 Rabbit pAb

A11720-50ul 50 ul
EUR 223

NACC1 Conjugated Antibody

C48470 100ul
EUR 397

NACC1 Blocking Peptide

BF0187-BP 1mg
EUR 195

anti-NACC1 (6H2)

LF-MA30500 100 ul
EUR 486
Description: Mouse Monoclonal to NACC1

Anti-NACC1 antibody

STJ113308 100 µl
EUR 277
Description: This gene encodes a member of the BTB/POZ protein family. BTB/POZ proteins are involved in several cellular processes including proliferation, apoptosis and transcription regulation. The encoded protein is a transcriptional repressor that plays a role in stem cell self-renewal and pluripotency maintenance. The encoded protein also suppresses transcription of the candidate tumor suppressor Gadd45GIP1, and expression of this gene may play a role in the progression of multiple types of cancer. A pseudogene of this gene is located on the short arm of chromosome 9.

Anti-NACC1 antibody

STJ113847 100 µl
EUR 277
Description: This gene encodes a member of the BTB/POZ protein family. BTB/POZ proteins are involved in several cellular processes including proliferation, apoptosis and transcription regulation. The encoded protein is a transcriptional repressor that plays a role in stem cell self-renewal and pluripotency maintenance. The encoded protein also suppresses transcription of the candidate tumor suppressor Gadd45GIP1, and expression of this gene may play a role in the progression of multiple types of cancer. A pseudogene of this gene is located on the short arm of chromosome 9.

NACC1 Protein Vector (Human) (pPB-C-His)

PV103486 500 ng
EUR 552

NACC1 Protein Vector (Human) (pPB-N-His)

PV103487 500 ng
EUR 552

NACC1 Protein Vector (Human) (pPM-C-HA)

PV103488 500 ng
EUR 552

NACC1 Protein Vector (Human) (pPM-C-His)

PV103489 500 ng
EUR 552

Polyclonal NACC1 Antibody (Center)

APR04758G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human NACC1 (Center). This antibody is tested and proven to work in the following applications:

Rat NACC1 shRNA Plasmid

20-abx987779
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Mouse NACC1 shRNA Plasmid

20-abx975804
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

NACC1 Recombinant Protein (Mouse)

RP152966 100 ug Ask for price

NACC1 Recombinant Protein (Rat)

RP213191 100 ug Ask for price

NACC1 sgRNA CRISPR Lentivector set (Human)

K1386401 3 x 1.0 ug
EUR 339

NACC1 Protein Vector (Mouse) (pPB-C-His)

PV203958 500 ng
EUR 603

NACC1 Protein Vector (Mouse) (pPB-N-His)

PV203959 500 ng
EUR 603

NACC1 Protein Vector (Mouse) (pPM-C-HA)

PV203960 500 ng
EUR 603

NACC1 Protein Vector (Mouse) (pPM-C-His)

PV203961 500 ng
EUR 603

NACC1 Protein Vector (Rat) (pPB-C-His)

PV284258 500 ng
EUR 603

NACC1 Protein Vector (Rat) (pPB-N-His)

PV284259 500 ng
EUR 603

NACC1 Protein Vector (Rat) (pPM-C-HA)

PV284260 500 ng
EUR 603

NACC1 Protein Vector (Rat) (pPM-C-His)

PV284261 500 ng
EUR 603

NACC1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV693128 1.0 ug DNA
EUR 682

NACC1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV693129 1.0 ug DNA
EUR 740

NACC1 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV693130 1.0 ug DNA
EUR 740

Polyclonal NACC1 Antibody (C-term)

APR04778G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human NACC1 (C-term). This antibody is tested and proven to work in the following applications:

NACC1 sgRNA CRISPR Lentivector (Human) (Target 1)

K1386402 1.0 ug DNA
EUR 154

NACC1 sgRNA CRISPR Lentivector (Human) (Target 2)

K1386403 1.0 ug DNA
EUR 154

NACC1 sgRNA CRISPR Lentivector (Human) (Target 3)

K1386404 1.0 ug DNA
EUR 154

Nucleus Accumbens Associated 1 (NACC1) Antibody

20-abx126214
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

Nucleus Accumbens Associated 1 (NACC1) Antibody

abx011231-100ul 100 ul
EUR 411
  • Shipped within 5-10 working days.

Nucleus Accumbens Associated 1 (NACC1) Antibody

abx330473-100ul 100 ul
EUR 425
  • Shipped within 5-10 working days.

Nacc1 sgRNA CRISPR Lentivector set (Mouse)

K4905601 3 x 1.0 ug
EUR 339

Nacc1 sgRNA CRISPR Lentivector set (Rat)

K7051401 3 x 1.0 ug
EUR 339

Human Nucleus accumbens- associated protein 1, NACC1 ELISA KIT

ELI-16141h 96 Tests
EUR 824

Nacc1 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4905602 1.0 ug DNA
EUR 154

Nacc1 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4905603 1.0 ug DNA
EUR 154

Nacc1 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4905604 1.0 ug DNA
EUR 154

Nacc1 sgRNA CRISPR Lentivector (Rat) (Target 1)

K7051402 1.0 ug DNA
EUR 154

Nacc1 sgRNA CRISPR Lentivector (Rat) (Target 2)

K7051403 1.0 ug DNA
EUR 154

Nacc1 sgRNA CRISPR Lentivector (Rat) (Target 3)

K7051404 1.0 ug DNA
EUR 154

Nacc1 3'UTR Luciferase Stable Cell Line

TU113804 1.0 ml Ask for price

Nacc1 3'UTR GFP Stable Cell Line

TU163804 1.0 ml Ask for price

Nacc1 3'UTR Luciferase Stable Cell Line

TU213713 1.0 ml Ask for price

Nacc1 3'UTR GFP Stable Cell Line

TU263713 1.0 ml Ask for price

NACC1 3'UTR GFP Stable Cell Line

TU065180 1.0 ml
EUR 1521

NACC1 3'UTR Luciferase Stable Cell Line

TU015180 1.0 ml
EUR 1521

NACC1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K1386405 3 x 1.0 ug
EUR 376

NACC1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K1386406 1.0 ug DNA
EUR 167

NACC1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K1386407 1.0 ug DNA
EUR 167

NACC1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K1386408 1.0 ug DNA
EUR 167

Mouse Nucleus accumbens- associated protein 1, Nacc1 ELISA KIT

ELI-38383m 96 Tests
EUR 865

Nacc1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K4905605 3 x 1.0 ug
EUR 376

Nacc1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K7051405 3 x 1.0 ug
EUR 376

Nacc1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K4905606 1.0 ug DNA
EUR 167

Nacc1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K4905607 1.0 ug DNA
EUR 167

Nacc1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K4905608 1.0 ug DNA
EUR 167

Nacc1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K7051406 1.0 ug DNA
EUR 167

A replicating plasmid-based vector for GFP expression in Mycoplasma hyopneumoniae.

Mycoplasma hyopneumoniae (M. hyopneumoniae) causes porcine enzootic pneumonia (PEP) that considerably impacts the pig trade worldwide. Regardless of the provision of the entire genome sequence, research on the pathogenesis of this organism have been restricted as a result of lack of a genetic manipulation system.
Subsequently, the intention of the present examine was to generate a common GFP reporter vector primarily based on a replicating plasmid. Right here, we describe the feasibility of GFP reporter expression in M. hyopneumoniae (pressure 168L) managed by the p97 gene promoter of this mycoplasma. An expression plasmid (pMD18-TOgfp) containing the p97 gene promoter, and origin of replication (oriC) of M. hyopneumoniae, tetracycline resistant marker (tetM), and GFP was constructed and used to rework competent M. hyopneumoniae cells.
We noticed inexperienced fluorescence in M. hyopneumoniae transformants beneath fluorescence microscopy, which signifies that there was expression of the GFP reporter that was pushed by the p97 gene promoter.
Moreover, an electroporation methodology for M. hyopneumoniae with an effectivity of roughly 1 x 10(-6) transformants/μg plasmid DNA was optimized and is described herein. In conclusion, our information display the susceptibility of M. hyopneumoniae to genetic manipulation whereby overseas genes are expressed. This work might encourage the event of genetic instruments to govern the genome of M. hyopneumoniae for practical genomic analyses.

Human adipose derived mesenchymal stromal cells transduced with GFP lentiviral vectors: evaluation of immunophenotype and differentiation capability in vitro.

Adipose derived mesenchymal stromal/stem cells (ASCs) are a heterogeneous inhabitants characterised by (a) their potential to stick to plastic; (b) immunophenotypic expression of sure cell floor markers, whereas missing others; and (c) the capability to distinguish into lineages of mesodermal origin together with osteocytes, chondrocytes and adipocytes. The long-term objective is to make the most of these cells for medical translation into cell-based therapies. Nonetheless, preclinical security and efficacy have to be demonstrated in animal fashions.
ASCs will also be utilized as organic autos for vector-based gene supply techniques, since they’re believed to dwelling to websites of irritation and an infection in vivo. These elements motivated the event of a labelling system for ASCs utilizing lentiviral vector-based inexperienced fluorescent protein (GFP) transduction. Human ASCs had been transduced with GFP-expressing lentiviral vectors. A titration examine decided the viral titer required to transduce the utmost variety of ASCs.
The impact of the transduced GFP lentiviral vector on ASC immunophenotypic expression of floor markers in addition to their potential to distinguish into osteocytes and adipocytes had been assessed in vitro.
Transduction effectivity in ASC cultures of roughly 80 % was noticed with an MOI of ~118. No important immunophenotypic variations had been noticed between transduced and non-transduced cells and each cell sorts efficiently differentiated into adipocytes and osteocytes in vitro. We obtained >80 % transduction of ASCs utilizing GFP lentiviral vectors.
Transduced ASCs maintained plastic adherence, demonstrated ASC immunophenotype and the flexibility to distinguish into cells of the mesodermal lineage. This GFP-ASC transduction approach provides a possible monitoring system for future pre-clinical research.
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