A set of GFP-based organelle marker strains mixed with DsRed-based gateway vectors for subcellular localization research in rice (Oryza sativa L.).
Within the post-genomic period, many helpful instruments have been developed to speed up the investigation of gene capabilities. Fluorescent proteins have been broadly used as protein tags for learning the subcellular localization of proteins in crops.
A number of fluorescent organelle marker strains have been generated in dicot crops; nonetheless, helpful and dependable fluorescent organelle marker strains are missing within the monocot mannequin rice.
Right here, we developed eight totally different GFP-based organelle markers in transgenic rice and created a set of DsRed-based gateway vectors for combining with the marker strains. Two mitochondrial-localized rice ascorbate peroxidase genes fused to DsRed and efficiently co-localized with mitochondrial-targeted marker strains verified the sensible use of this method. The co-localization of GFP-fusion marker strains and DsRed-fusion proteins present a handy platform for in vivo or in vitro evaluation of subcellular localization of rice proteins
gfpvector
 IGJ antibody |
|||
| 10R-6992 | Fitzgerald | 100 ul | EUR 691 |
|
Description: Mouse monoclonal IGJ antibody |
|||
IGJ antibody |
|||
| 10R-6994 | Fitzgerald | 100 ul | EUR 691 |
|
Description: Mouse monoclonal IGJ antibody |
|||
IGJ antibody |
|||
| 10R-6995 | Fitzgerald | 100 ul | EUR 691 |
|
Description: Mouse monoclonal IGJ antibody |
|||
IGJ antibody |
|||
| 10R-4454 | Fitzgerald | 100 ul | EUR 691 |
|
Description: Mouse monoclonal IGJ antibody |
|||
IGJ antibody |
|||
| 10R-4455 | Fitzgerald | 100 ul | EUR 726 |
|
Description: Mouse monoclonal IGJ antibody |
|||
IGJ antibody |
|||
| 10R-4456 | Fitzgerald | 100 ul | EUR 691 |
|
Description: Mouse monoclonal IGJ antibody |
|||
 anti-IGJ |
|||
| YF-PA12640 | Abfrontier | 50 ug | EUR 363 |
|
Description: Mouse polyclonal to IGJ |
|||
 Human IGJ ELISA Kit |
|||
| ELA-E0643h | Lifescience Market | 96 Tests | EUR 824 |
 IGJ ELISA KIT|Human |
|||
| EF000679 | Lifescience Market | 96 Tests | EUR 689 |
 Human IGJ shRNA Plasmid |
|||
| 20-abx952364 | Abbexa |
|
|
|
|||
) IGJ Recombinant Protein (Human) |
|||
| RP015793 | ABM | 100 ug | Ask for price |
 (pORF)) Igj ORF Vector (Mouse) (pORF) |
|||
| ORF047749 | ABM | 1.0 ug DNA | EUR 506 |
 IGJ cloning plasmid |
|||
| CSB-CL011337HU-10ug | Cusabio | 10ug | EUR 244 |
|
|||
|
Description: A cloning plasmid for the IGJ gene. |
|||
 anti- IGJ antibody |
|||
| FNab04187 | FN Test | 100µg | EUR 505.25 |
|
|||
|
Description: Antibody raised against IGJ |
|||
 Anti-IGJ antibody |
|||
| PAab04187 | Lifescience Market | 100 ug | EUR 355 |
 (pPB-C-His)) IGJ Protein Vector (Human) (pPB-C-His) |
|||
| PV021057 | ABM | 500 ng | EUR 329 |
 (pPB-N-His)) IGJ Protein Vector (Human) (pPB-N-His) |
|||
| PV021058 | ABM | 500 ng | EUR 329 |
 (pPM-C-HA)) IGJ Protein Vector (Human) (pPM-C-HA) |
|||
| PV021059 | ABM | 500 ng | EUR 329 |
 (pPM-C-His)) IGJ Protein Vector (Human) (pPM-C-His) |
|||
| PV021060 | ABM | 500 ng | EUR 329 |
) IGJ protein (His tag) |
|||
| 80R-2743 | Fitzgerald | 100 ug | EUR 322 |
|
Description: Purified recombinant IGJ protein (His tag) |
|||
 Mouse IGJ shRNA Plasmid |
|||
| 20-abx970998 | Abbexa |
|
|
|
|||
) IGJ Recombinant Protein (Mouse) |
|||
| RP143243 | ABM | 100 ug | Ask for price |
 Rabbit Anti-Human IGJ Monoclonal Antibody |
|||
| CABT-29847RH | Creative Diagnostics | 100 ul | EUR 840 |
 Antibody) Human Immunoglobulin J Chain (IGJ) Antibody |
|||
| 30165-05111 | AssayPro | 150 ug | EUR 261 |
) IGJ sgRNA CRISPR Lentivector set (Human) |
|||
| K1049501 | ABM | 3 x 1.0 ug | EUR 339 |
 Rabbit Anti-Human IGJ Polyclonal Antibody |
|||
| CPBT-36211RH | Creative Diagnostics | 100ug | EUR 840 |
 IGJ Immunoglobulin J Human Recombinant Protein |
|||
| PROTP01591 | BosterBio | Regular: 20ug | EUR 317 |
|
Description: IGJ Human Recombinant produced in E. coli is a single polypeptide chain containing 160 amino acids (23-159) and having a molecular mass of 18kDa. IGJ is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
|||
 (pPB-C-His)) IGJ Protein Vector (Mouse) (pPB-C-His) |
|||
| PV190994 | ABM | 500 ng | EUR 603 |
 (pPB-N-His)) IGJ Protein Vector (Mouse) (pPB-N-His) |
|||
| PV190995 | ABM | 500 ng | EUR 603 |
 (pPM-C-HA)) IGJ Protein Vector (Mouse) (pPM-C-HA) |
|||
| PV190996 | ABM | 500 ng | EUR 603 |
 (pPM-C-His)) IGJ Protein Vector (Mouse) (pPM-C-His) |
|||
| PV190997 | ABM | 500 ng | EUR 603 |
 Antibody) Immunoglobulin J Chain (IGJ) Antibody |
|||
| abx234187-100ug | Abbexa | 100 ug | EUR 481 |
|
|||
) Mouse Immunoglobulin J chain (Igj) |
|||
| 1-CSB-YP011337MO | Cusabio |
|
|
|
|||
|
Description: Recombinant Mouse Immunoglobulin J chain(Igj) expressed in Yeast |
|||
 ELISA Kit) Human IGJ(Immunoglobulin J chain) ELISA Kit |
|||
| EH0989 | FN Test | 96T | EUR 567.6 |
|
|||
|
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml |
|||
 Human Immunoglobulin J chain, IGJ ELISA KIT |
|||
| ELI-02275h | Lifescience Market | 96 Tests | EUR 824 |
 (Target 1)) IGJ sgRNA CRISPR Lentivector (Human) (Target 1) |
|||
| K1049502 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 2)) IGJ sgRNA CRISPR Lentivector (Human) (Target 2) |
|||
| K1049503 | ABM | 1.0 ug DNA | EUR 154 |
 (Target 3)) IGJ sgRNA CRISPR Lentivector (Human) (Target 3) |
|||
| K1049504 | ABM | 1.0 ug DNA | EUR 154 |
 ELISA kit) Human Immunoglobulin J chain(IGJ) ELISA kit |
|||
| CSB-E09237h-24T | Cusabio | 1 plate of 24 wells | EUR 165 |
|
|||
|
Description: Quantitativesandwich ELISA kit for measuring Human Immunoglobulin J chain (IGJ) in samples from serum, plasma, saliva, urine, tear, breastmilk. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
|||
Human Immunoglobulin J chain(IGJ) ELISA kit |
|||
| 1-CSB-E09237h | Cusabio |
|
|
|
|||
|
Description: Quantitativesandwich ELISA kit for measuring Human Immunoglobulin J chain(IGJ) in samples from serum, plasma, saliva, urine, tear, breastmilk. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
|||
 Recombinant Human IGJ Protein, His, E.coli-1mg |
|||
| QP12387-1mg | EnQuireBio | 1mg | EUR 2757 |
 Recombinant Human IGJ Protein, His, E.coli-20ug |
|||
| QP12387-20ug | EnQuireBio | 20ug | EUR 201 |
 Recombinant Human IGJ Protein, His, E.coli-5ug |
|||
| QP12387-5ug | EnQuireBio | 5ug | EUR 155 |
) Igj sgRNA CRISPR Lentivector set (Mouse) |
|||
| K3363801 | ABM | 3 x 1.0 ug | EUR 339 |
 AssayMax ELISA Kit) Human Immunoglobulin J Chain (IGJ) AssayMax ELISA Kit |
|||
| EI1701-1 | AssayPro | 96 Well Plate | EUR 417 |
 ELISA Kit) Human Immunoglobulin J chain / IGJ (JCHAIN) ELISA Kit |
|||
| abx253943-96tests | Abbexa | 96 tests | EUR 739 |
|
|||
 Antibody (Biotin Conjugate)) Human Immunoglobulin J Chain (IGJ) Antibody (Biotin Conjugate) |
|||
| 30165-05121 | AssayPro | 150 ug | EUR 369 |
) Rabbit Anti-Human IGJ Polyclonal Antibody(Pre-diluted) |
|||
| CPBT-36213RH | Creative Diagnostics | 7ml | EUR 991 |
 (CMV) (pLenti-GIII-CMV)) RNU6V Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV746825 | ABM | 1.0 ug DNA | Ask for price |
 (UbC) (pLenti-GIII-UbC)) RNU6V Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV746829 | ABM | 1.0 ug DNA | Ask for price |
 (EF1a) (pLenti-GIII-EF1a)) RNU6V Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
|||
| LV746830 | ABM | 1.0 ug DNA | Ask for price |
 (CMV) (pLenti-GIII-CMV)) RNY1P1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV746849 | ABM | 1.0 ug DNA | Ask for price |
 (UbC) (pLenti-GIII-UbC)) RNY1P1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV746853 | ABM | 1.0 ug DNA | Ask for price |
 (EF1a) (pLenti-GIII-EF1a)) RNY1P1 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
|||
| LV746854 | ABM | 1.0 ug DNA | Ask for price |
 (CMV) (pLenti-GIII-CMV)) RNY1P2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV746855 | ABM | 1.0 ug DNA | Ask for price |
 (UbC) (pLenti-GIII-UbC)) RNY1P2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV746859 | ABM | 1.0 ug DNA | Ask for price |
 (EF1a) (pLenti-GIII-EF1a)) RNY1P2 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
|||
| LV746860 | ABM | 1.0 ug DNA | Ask for price |
 (CMV) (pLenti-GIII-CMV)) RNY1P3 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV746861 | ABM | 1.0 ug DNA | Ask for price |
 (UbC) (pLenti-GIII-UbC)) RNY1P3 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV746865 | ABM | 1.0 ug DNA | Ask for price |
 (EF1a) (pLenti-GIII-EF1a)) RNY1P3 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
|||
| LV746866 | ABM | 1.0 ug DNA | Ask for price |
 (CMV) (pLenti-GIII-CMV)) RNY1P4 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV746867 | ABM | 1.0 ug DNA | Ask for price |
 (UbC) (pLenti-GIII-UbC)) RNY1P4 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV746871 | ABM | 1.0 ug DNA | Ask for price |
 (EF1a) (pLenti-GIII-EF1a)) RNY1P4 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
|||
| LV746872 | ABM | 1.0 ug DNA | Ask for price |
 (CMV) (pLenti-GIII-CMV)) RNY1P5 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV746873 | ABM | 1.0 ug DNA | Ask for price |
 (UbC) (pLenti-GIII-UbC)) RNY1P5 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV746877 | ABM | 1.0 ug DNA | Ask for price |
 (EF1a) (pLenti-GIII-EF1a)) RNY1P5 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
|||
| LV746878 | ABM | 1.0 ug DNA | Ask for price |
 (CMV) (pLenti-GIII-CMV)) RNY1P6 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV746879 | ABM | 1.0 ug DNA | Ask for price |
 (UbC) (pLenti-GIII-UbC)) RNY1P6 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV746883 | ABM | 1.0 ug DNA | Ask for price |
 (EF1a) (pLenti-GIII-EF1a)) RNY1P6 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
|||
| LV746884 | ABM | 1.0 ug DNA | Ask for price |
 (CMV) (pLenti-GIII-CMV)) RNY1P7 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV746885 | ABM | 1.0 ug DNA | Ask for price |
 (UbC) (pLenti-GIII-UbC)) RNY1P7 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV746889 | ABM | 1.0 ug DNA | Ask for price |
 (EF1a) (pLenti-GIII-EF1a)) RNY1P7 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
|||
| LV746890 | ABM | 1.0 ug DNA | Ask for price |
 (CMV) (pLenti-GIII-CMV)) RNY1P8 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV746891 | ABM | 1.0 ug DNA | Ask for price |
 (UbC) (pLenti-GIII-UbC)) RNY1P8 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV746895 | ABM | 1.0 ug DNA | Ask for price |
 (EF1a) (pLenti-GIII-EF1a)) RNY1P8 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
|||
| LV746896 | ABM | 1.0 ug DNA | Ask for price |
 (CMV) (pLenti-GIII-CMV)) RNY3P1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV746897 | ABM | 1.0 ug DNA | Ask for price |
 (UbC) (pLenti-GIII-UbC)) RNY3P1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV746901 | ABM | 1.0 ug DNA | Ask for price |
 (EF1a) (pLenti-GIII-EF1a)) RNY3P1 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
|||
| LV746902 | ABM | 1.0 ug DNA | Ask for price |
 (CMV) (pLenti-GIII-CMV)) RNY3P2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
|||
| LV746903 | ABM | 1.0 ug DNA | Ask for price |
 (UbC) (pLenti-GIII-UbC)) RNY3P2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
|||
| LV746907 | ABM | 1.0 ug DNA | Ask for price |
Temporal characterization of protein manufacturing ranges from baculovirus vectors coding for GFP and RFP genes beneath non-conventional promoter management.
The convenience of use and flexibility of the Baculovirus Expression Vector System (BEVS) has made it some of the broadly used techniques for recombinant protein manufacturing Nevertheless, co-expression techniques presently in use primarily make use of the very sturdy very late p10 and polyhedron (polh) promoters to drive expression of overseas genes, which doesn’t present a lot scope for tailoring expression ratios inside the cell.
This work demonstrates using totally different Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) promoters to manage the timing and expression of two simply traceable fluorescent proteins, the improved inexperienced fluorescent protein (eGFP), and a crimson fluorescent protein (DsRed2) in a BEVS co-expression system.
Our outcomes present that gene expression ranges can simply be managed utilizing this technique, and likewise that modulating the expression stage of 1 protein can affect the extent of expression of the opposite protein inside the system, thus confirming the idea of genes “competing” for restricted mobile assets. Plots of “expression ratios” of the 2 mannequin genes over time have been obtained, and could also be utilized in future work to tightly management timing and ranges of overseas gene expression in an insect cell co-expression system.
A novel binary T-vector with the GFP reporter gene for promoter characterization.
A number of methods have been developed to clone PCR fragments into desired vectors. Nevertheless, most of commercially accessible T-vectors aren’t binary vectors and can’t be straight used for Agrobacterium-mediated plant genetic transformation.
On this research, a novel binary T-vector was constructed by integrating two AhdI restriction websites into the spine vector pCAMBIA 1300. The T-vector additionally incorporates a GFP reporter gene and thus, can be utilized to investigate promoter exercise by monitoring the reporter gene. Alternatively, identification and characterization of varied promoters not solely profit the practical annotation of their genes but in addition present different candidates for use to drive attention-grabbing genes for plant genetic enchancment by transgenesis.
Greater than 1,000 putative pollen-specific rice genes have been recognized in a genome-wide stage. Amongst them, 67 extremely expressed genes have been additional characterised. One of many pollen-specific genes LOC_Os10g35930 was additional surveyed in its expression patterns with extra particulars by quantitative real-time reverse-transcription PCR (qRT-PCR) evaluation.
Lastly, its promoter exercise was additional investigated by analyzing transgenic rice crops carrying the promoter::GFP cassette, which was constructed from the newly developed T-vector. The reporter GFP gene expression in these transgenic crops confirmed that the promoter was lively solely in mature however not in germinated pollens.
Product not found