
Plant viruses will be engineered to hold sequences that direct silencing of goal host genes, expression of heterologous proteins, or modifying of host genes. A set of foxtail mosaic virus (FoMV) vectors was developed that can be utilized for transient gene expression and single information RNA supply for Cas9-mediated gene modifying in maize, Setaria viridis, and Nicotiana benthamiana.
This was achieved by duplicating the FoMV capsid protein subgenomic promoter, abolishing the pointless open studying body 5A, and inserting a cloning website containing distinctive restriction endonuclease cleavage websites instantly after the duplicated promoter. The modified FoMV vectors transiently expressed inexperienced fluorescent protein (GFP) and bialaphos resistance (BAR) protein in leaves of systemically contaminated maize seedlings. GFP was detected in epidermal and mesophyll cells by epifluorescence microscopy, and expression was confirmed by Western blot analyses. Crops contaminated with FoMV carrying the bar gene had been briefly shielded from a glufosinate herbicide, and expression was confirmed utilizing a speedy antibody-based BAR strip take a look at. Expression of those proteins was stabilized by nucleotide substitutions within the sequence of the duplicated promoter area.
Single information RNAs expressed from the duplicated promoter mediated edits within the N. benthamiana Phytoene desaturase gene, the S. viridis Carbonic anhydrase 2 gene, and the maize HKT1 gene encoding a potassium transporter. The effectivity of modifying was enhanced within the presence of synergistic viruses and a viral silencing suppressor. This work expands the utility of FoMV for virus-induced gene silencing (VIGS), virus-mediated overexpression (VOX), and virus-enabled gene modifying (VEdGE) in monocots.
A brand new toolkit for gene tagging in Candida albicans containing recyclable markers.
Gene manipulation and epitope tagging are important instruments for understanding the molecular perform of particular genes. The opportunistic human pathogen Candida albicans is a diploid fungus that makes use of a non-canonical genetic code. Since choice markers out there on this organism are scarce, a number of instruments primarily based on recyclable markers have been developed for gene disruption, such because the Clox system.
This technique depends on the Cre recombinase, which recycles choice markers flanked by loxP websites with excessive effectivity, facilitating single marker or multi-marker recycling. Nevertheless, PCR-based modules for epitope tagging, such the pFA-modules, primarily use restricted non-recyclable auxotrophic markers. To unravel this downside, now we have used a Gibson meeting technique to assemble a set of recent plasmids the place the auxotrophic markers of the pFA vectors had been swapped with 5 recyclable marker modules of the Clox system, enhancing the flexibility of the pFA plasmids. This new toolkit, named pFA-Clox, consists of 36 new vectors for gene disruption and epitope tagging (GFP, 3xGFP, mCherry, 3xHA, 5xmyc and TAP). These plasmids include the dominant NAT1 marker, in addition to URA3, HIS1 and ARG4 cassettes, thereby allowing useful evaluation of laboratory strains in addition to scientific isolates of C. albicans. In abstract, now we have tailored the Clox system to the pFA-backbone vectors.
Thus, the set of primers used for the amplification of beforehand printed pFA modules may also be utilized on this new pFA-Clox system. Subsequently, this new toolkit harbors some great benefits of each techniques, permitting accelerated gene modification methods that might cut back time and prices in pressure building for C. albicans. Antitumor immunotherapeutic methods characterize an particularly promising set of approaches with speedy translational potential contemplating the dismal scientific context of high-grade gliomas. Dendritic cells (DCs) are the physique’s {most professional} antigen-presenting cells, capable of recruit and activate T cells to stimulate an adaptive immune response. On this regard, particular loading of tumor-specific antigen onto dendritic cells doubtlessly represents one of the vital superior methods to attain efficient antitumor immunization.

Protein expression and gene editing in monocots using foxtail mosaic virus vectors.
Chlamydia trachomatis and Chlamydia muridarum spectinomycin resistant vectors and a transcriptional fluorescent reporter to observe conversion from replicative to infectious micro organism.
PUCM-T Cloning Vector (50ng/ul) |
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BS434 | Bio Basic | 5UG | EUR 185.28 |
pcDNA3 GFP LIC cloning vector (6D) |
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PVT10748 | Lifescience Market | 2 ug | EUR 361.2 |
pTG19-T PCR Cloning Vector, 20apps |
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TA010 | Vivantis | each | Ask for price |
pLenti-III-mir-GFP Cloning Vector |
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m016 | ABM | 500 ng | EUR 725 |
pET His6 MBP TEV LIC cloning vector (1M) |
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PVT10596 | Lifescience Market | 2 ug | EUR 319.2 |
pET His6 Sumo TEV LIC cloning vector (1S) |
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PVT14034 | Lifescience Market | 2 ug | EUR 718.8 |
pET His6 Sumo TEV LIC cloning vector (2S-T) |
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PVT14035 | Lifescience Market | 2 ug | EUR 718.8 |
pFastBac Dual His6 TEV LIC cloning vector(5B) Plasmid |
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PVT43314 | Nova Lifetech | 2ug | EUR 280 |
pFastBac His6 TEV cloning vector with BioBrick polycistronic |
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PVT29881 | Nova Lifetech | 2ug | EUR 280 |
pCDF1-MCS1 cDNA Cloning and Expression Vector |
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CD100A-1 | SBI | 10 ug | EUR 483 |
pCDH-CMV-MCS cDNA Cloning and Expression Vector |
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CD500B-1 | SBI | 10 ug | EUR 483 |
pCDH-EF1-MCS cDNA Cloning and Expression Vector |
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CD502A-1 | SBI | 10 ug | EUR 483 |
pCDH-CMV-MCS2 cDNA Cloning and Expression Vector |
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CD501A-1 | SBI | 10 ug | EUR 483 |
pSIH1-H1-Puro shRNA Cloning and Expression Vector |
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SI500A-1 | SBI | 10 ug | EUR 507 |
pSIH1-H1-H2Kk shRNA Cloning and Expression Vector |
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SI502A-1 | SBI | 10 ug | EUR 507 |
pAAVK-EF1α -MCS AAVanced Cloning and Expression Vector |
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AAV502A-1 | SBI | 10 ug | EUR 614 |
pSIH1-H1-copGFP shRNA Cloning and Expression Vector |
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SI501A-1 | SBI | 10 ug | EUR 507 |
Cumate-Inducible (CuO) EEV cloning and expression vector |
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EEV610A-1 | SBI | 10 ug | EUR 614 |
Green Kit. Baculovirus GFP vector. |
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K20 | AB Vector LLC | 1 Kit | EUR 695 |
Description: Protein expression |
ProGreen. Baculovirus GFP marker vector. |
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A1 | AB Vector LLC | 25 ul | EUR 420 |
Description: Protein expression |
pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector |
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CD811A-1 | SBI | 10 ug | EUR 579 |
pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector |
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CD810A-1 | SBI | 10 ug | EUR 579 |
Constitutive (CAGs promoter) EEV cloning and expression vector |
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EEV600A-1 | SBI | 10 ug | EUR 614 |
pCDH-MCS-T2A-copGFP-MSCV Cloning and Expression Vector |
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CD523A-1 | SBI | 10 ug | EUR 590 |
pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector |
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CD111B-1 | SBI | 10 ug | EUR 572 |
PB-CMV-MCS-EF1-GFP cDNA cloning and expression vector |
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PB511B-1 | SBI | 10 ug | EUR 638 |
PB-CMV-MCS-EF1-RFP cDNA cloning and expression vector |
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PB512B-1 | SBI | 10 ug | EUR 638 |
PB-CMV-MCS-EF1-Puro cDNA cloning and expression vector |
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PB510B-1 | SBI | 10 ug | EUR 638 |
PB-EF1-MCS-IRES-GFP cDNA cloning and expression vector |
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PB530A-2 | SBI | 10 ug | EUR 638 |
PB-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
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PB531A-2 | SBI | 10 ug | EUR 638 |
PB-EF1-MCS-IRES-Neo cDNA cloning and expression vector |
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PB533A-2 | SBI | 10 ug | EUR 638 |
pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector |
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CD512B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector |
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CD514B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
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CD510B-1 | SBI | 10 ug | EUR 572 |
pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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CD530A-2 | SBI | 10 ug | EUR 590 |
pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector |
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CD531A-2 | SBI | 10 ug | EUR 590 |
pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector |
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CD533A-2 | SBI | 10 ug | EUR 590 |
pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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CD630A-1 | SBI | 10 ug | EUR 590 |
pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector |
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CD711B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
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CD515B-1 | SBI | 10 ug | EUR 572 |
pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector |
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CD522A-1 | SBI | 10 ug | EUR 590 |
pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector |
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CD532A-2 | SBI | 10 ug | EUR 590 |
pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector |
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CD615B-1 | SBI | 10 ug | EUR 572 |
pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector |
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CD710B-1 | SBI | 10 ug | EUR 572 |
pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector |
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CD731B-1 | SBI | 10 ug | EUR 590 |
PB-CMV-MCS-EF1-RedPuro cDNA Cloning and Expression Vector |
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PB514B-2 | SBI | 10 ug | EUR 638 |
ProEasy. Vector for easy construction of recombinant baculoviruses. |
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A10S | AB Vector LLC | 25 ul | EUR 695 |
Description: Protein expression |
pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector |
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CD511B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector |
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CD524A-1 | SBI | 10 ug | EUR 590 |
PinPoint-HR attP Placement Vector – Empty MCS for cloning HR Arms |
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PIN400A-1 | SBI | 10ug | EUR 1190 |
PB-CMV-MCS-EF1-GreenPuro cDNA cloning and expression vector |
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PB513B-1 | SBI | 10 ug | EUR 638 |
PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector |
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PBSI505A-1 | SBI | 10 ug | EUR 638 |
PB-EF1-GreenPuro-H1-MCS shRNA cloning and expression vector |
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PBSI506A-1 | SBI | 10 ug | EUR 638 |
pAAVK-EF1α -MCS-T2A-EGFP AAVanced Cloning and Expression Vector |
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AAV526A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α -MCS-T2A-Puro AAVanced Cloning and Expression Vector |
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AAV527A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α –MCS-T2A-mRFP AAVanced Cloning and Expression Vector |
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AAV528A-1 | SBI | 10 ug | EUR 614 |
PB-MSCV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
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PB713B-1 | SBI | 10 ug | EUR 638 |
pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector |
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CD513B-1 | SBI | 10 ug | EUR 572 |
pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector |
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CD516B-2 | SBI | 10 ug | EUR 572 |
pAB-bee. Baculovirus plasmid vector for secreted and transmembrane proteins. |
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B3 | AB Vector LLC | 50 ul | EUR 495 |
Description: Protein expression |
pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector |
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CD713B-1 | SBI | 10 ug | EUR 572 |
pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector |
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CD813A-1 | SBI | 10 ug | EUR 579 |
pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector |
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CD822A-1 | SBI | 10 ug | EUR 579 |
pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector |
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CD823A-1 | SBI | 10 ug | EUR 579 |
pAAVK-EF1α-MCS1-CMV-MCS2 AAVanced Cloning and Expression Vector |
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AAV503A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α-MCS1-CMV-EGFP AAVanced Cloning and Expression Vector |
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AAV536A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α-MCS1-CMV-Puro AAVanced Cloning and Expression Vector |
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AAV537A-1 | SBI | 10 ug | EUR 614 |
pAAVK-EF1α-MCS1-CMV-mRFP AAVanced Cloning and Expression Vector |
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AAV538A-1 | SBI | 10 ug | EUR 614 |
pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector 10 µg |
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CD527A-1 | SBI | 10 ug | EUR 590 |
ProFold-PDI. Baculovirus chaperone vector for expression of cysteine-rich proteins. |
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A7 | AB Vector LLC | 25 ul | EUR 830 |
Description: Protein expression |
gRNA_Cloning Vector |
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PVTY00131 | Nova Lifetech | 2ug | EUR 280 |
ProFold-C2. Baculovirus chaperone vector for expression of cytoplasmic and nuclear proteins. |
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A3 | AB Vector LLC | 25 ul | EUR 830 |
Description: Protein expression |
ProFold-ER1. Baculovirus chaperone vector for expression of secreted and membrane proteins. |
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A4 | AB Vector LLC | 25 ul | EUR 830 |
Description: Protein expression |
ProFold-C1. Baculovirus chaperone vector for expression of cytoplasmic and nuclear proteins. |
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A2 | AB Vector LLC | 25 ul | EUR 830 |
Description: Protein expression |
pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector |
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CD550A-1 | SBI | 10 ug | EUR 563 |
PB-CuO-CMV-MCS-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector |
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PBQM800A-1 | SBI | 10 µg | EUR 921 |
PB-Cuo-MCS-IRES-GFP-EF1-CymR-Puro Inducible cDNA Cloning and Expression Vector |
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PBQM812A-1 | SBI | 10 ug | EUR 1023 |
Cloning |
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S201 | 101Bio | - | Ask for price |
SIRT7 cloning plasmid |
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CSB-CL885703HU-10ug | Cusabio | 10ug | EUR 536.4 |
Description: A cloning plasmid for the SIRT7 gene. |
SIRT4 cloning plasmid |
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CSB-CL897587HU-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the SIRT4 gene. |
SIRT2 cloning plasmid |
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CSB-CL812889HU-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the SIRT2 gene. |
SIRT1 cloning plasmid |
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CSB-CL822202HU-10ug | Cusabio | 10ug | EUR 451.2 |
Description: A cloning plasmid for the SIRT1 gene. |
SIRPA cloning plasmid |
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CSB-CL021334HU-10ug | Cusabio | 10ug | EUR 644.4 |
Description: A cloning plasmid for the SIRPA gene. |
SIRPD cloning plasmid |
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CSB-CL021337HU-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the SIRPD gene. |
SIRPG cloning plasmid |
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CSB-CL021338HU1-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the SIRPG gene. |
SIRPG cloning plasmid |
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CSB-CL021338HU2-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the SIRPG gene. |
SIRT6 cloning plasmid |
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CSB-CL854057HU1-10ug | Cusabio | 10ug | EUR 490.8 |
Description: A cloning plasmid for the SIRT6 gene. |
SIRT6 cloning plasmid |
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CSB-CL854057HU2-10ug | Cusabio | 10ug | EUR 462 |
Description: A cloning plasmid for the SIRT6 gene. |
SIRT6 cloning plasmid |
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CSB-CL854057HU3-10ug | Cusabio | 10ug | EUR 390 |
Description: A cloning plasmid for the SIRT6 gene. |
SIRPB1 cloning plasmid |
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CSB-CL021335HU-10ug | Cusabio | 10ug | EUR 314.4 |
Description: A cloning plasmid for the SIRPB1 gene. |
PB-Cuo-shMCS-IRES-GFP-EF1-CymR-Puro Inducible shRNA Cloning and Expression Vector |
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PBQMSH812A-1 | SBI | 10 ug | EUR 1023 |
C1 Kit. Baculovirus chaperone vectors for cytoplasmic and nuclear proteins. |
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K21 | AB Vector LLC | 1 Kit | EUR 995 |
Description: Protein expression |
C2 Kit. Baculovirus chaperone vectors for cytoplasmic and nuclear proteins. |
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K22 | AB Vector LLC | 1 Kit | EUR 995 |
Description: Protein expression |
ER1 Kit. Baculovirus chaperone vectors for expression of secreted and membrane proteins. |
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K23 | AB Vector LLC | 1 Kit | EUR 995 |
Description: Protein expression |
ER1-bee Kit. Baculovirus chaperone vectors for expression of secreted and membrane proteins. |
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K24 | AB Vector LLC | 1 Kit | EUR 995 |
Description: Protein expression |
gRNA- Cloning |
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PVT10957 | Lifescience Market | 2 ug | EUR 361.2 |
Cloning Optimizer |
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E004 | ABM | 200 μl | EUR 105 |
Description: Cloning Optimizer consists of a proprietary enzyme that can completely eliminate background colonies when cloning. Treatment of PCR amplified DNA with this innovative optimizer will remove the trace amounts of plasmid template DNA that remain after PCR. Cloning Optimizer eliminates non-linearized template plasmids that cause false-positive colonies. |
Cloning Optimizer |
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MBS4156417-02mL | MyBiosource | 0.2mL | EUR 245 |
Cloning Optimizer |
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MBS4156417-5x02mL | MyBiosource | 5x0.2mL | EUR 795 |
T1 Cloning Kit |
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abx098055-100l | Abbexa | 100 µl | EUR 737.5 |
T1 Cloning Kit |
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abx098055-1ml | Abbexa | 1 ml | Ask for price |
T1 Cloning Kit |
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abx098055-200l | Abbexa | 200 µl | EUR 950 |
T1 Cloning Kit |
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20-abx098055 | Abbexa |
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T3 Cloning Kit |
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abx098057-100l | Abbexa | 100 µl | EUR 737.5 |
T3 Cloning Kit |
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abx098057-1ml | Abbexa | 1 ml | Ask for price |
T3 Cloning Kit |
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abx098057-200l | Abbexa | 200 µl | EUR 950 |
T3 Cloning Kit |
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20-abx098057 | Abbexa |
|
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PCR Cloning<1kb |
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GC0018 | GenCefe Co Ltd | <1kb | EUR 59 |
Blunt Cloning Kit |
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abx098059-100l | Abbexa | 100 µl | EUR 737.5 |
Blunt Cloning Kit |
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abx098059-1ml | Abbexa | 1 ml | Ask for price |
Blunt Cloning Kit |
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abx098059-200l | Abbexa | 200 µl | EUR 950 |
Blunt Cloning Kit |
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20-abx098059 | Abbexa |
|
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IK cloning plasmid |
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CSB-CL613381HU-10ug | Cusabio | 10ug | EUR 451.2 |
Description: A cloning plasmid for the IK gene. |
XG cloning plasmid |
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CSB-CL026190HU-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the XG gene. |
XK cloning plasmid |
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CSB-CL026198HU-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the XK gene. |
GK cloning plasmid |
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CSB-CL300097HU-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the GK gene. |
Blunt3 Cloning Kit |
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abx098061-100l | Abbexa | 100 µl | EUR 737.5 |
Blunt3 Cloning Kit |
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abx098061-1ml | Abbexa | 1 ml | Ask for price |
Blunt3 Cloning Kit |
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abx098061-200l | Abbexa | 200 µl | EUR 950 |
Blunt3 Cloning Kit |
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20-abx098061 | Abbexa |
|
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C5 cloning plasmid |
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CSB-CL003995HU1-10ug | Cusabio | 10ug | EUR 3324 |
Description: A cloning plasmid for the C5 gene. |
C5 cloning plasmid |
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CSB-CL003995HU2-10ug | Cusabio | 10ug | EUR 3324 |
Description: A cloning plasmid for the C5 gene. |
C6 cloning plasmid |
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CSB-CL004036HU-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the C6 gene. |
C7 cloning plasmid |
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CSB-CL004145HU-10ug | Cusabio | 10ug | EUR 669.6 |
Description: A cloning plasmid for the C7 gene. |
C9 cloning plasmid |
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CSB-CL004259HU-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the C9 gene. |
CS cloning plasmid |
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CSB-CL006031HU1-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the CS gene. |
CS cloning plasmid |
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CSB-CL006031HU2-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the CS gene. |
F2 cloning plasmid |
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CSB-CL007923HU-10ug | Cusabio | 10ug | EUR 759.6 |
Description: A cloning plasmid for the F2 gene. |
F3 cloning plasmid |
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CSB-CL007928HU-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the F3 gene. |
F7 cloning plasmid |
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CSB-CL007930HU-10ug | Cusabio | 10ug | EUR 579.6 |
Description: A cloning plasmid for the F7 gene. |
F8 cloning plasmid |
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CSB-CL007932HU-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the F8 gene. |
F9 cloning plasmid |
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CSB-CL007936HU-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the F9 gene. |