Cap analog and Potato virus A HC-Professional silencing suppressor enhance GFP transient expression utilizing an infectious virus vector in Nicotiana benthamiana.
Transient expression of proteins in crops has grow to be a option to facilitate recombinant protein manufacturing with its quick and simple utility. However, host defensive mechanisms have been reported to cut back the effectivity of transient expression in crops. Therefore, this examine was designed to guage the impact of cap analog and Potato virus A helper element proteinase (PVA HC-Professional) on inexperienced fluorescent protein (GFP) expression effectivity. N. benthamiana leaves have been inoculated with capped or un-capped RNA transcripts of a Turnip crinkle virus (TCV) assemble containing a inexperienced fluorescent protein reporter gene (TCV-sGFP) instead of its coat protein (CP) ORF. PVA HC-Professional as a viral suppressor of RNA silencing was infiltrated in trans by Agrobacterium tumefaciens, elevated the GFP foci diameter to 6 and much more cells in each capped and un capped therapies.
The expression degree of GFP in inoculated crops with TCV-sGFP transcript pre-infiltrated with PVA HC-Professional was 12.97-fold increased than the GFP accumulation degree in pre-infiltrated leaves with empty plasmid (EP) management.
Additionally, the yield of GFP in inoculated N. benthamiana crops with capped TCV-sGFP transcript pre-infiltrated with EP and PVA HC-Professional was 1.54 and 1.2-fold respectively, larger than the extent of GFP expressed with out cap analog utility at 5 days publish inoculation (dpi). As well as, the motion of TCV-sGFP was elevated in some cells of inoculated leaves with capped transcripts. Outcomes of this examine indicated that PVA HC-Professional and mRNA capping can enhance GFP expression and its cell to cell motion in N. benthamiana.
gfpvector
 ADIPOQ Antibody |
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| 31141-100ul | SAB | 100ul | EUR 252 |
ADIPOQ Antibody |
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| 31141-50ul | SAB | 50ul | EUR 187 |
 ADIPOQ antibody |
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| 38421-100ul | SAB | 100ul | EUR 252 |
ADIPOQ Antibody |
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| CSB-PA828343- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against ADIPOQ. Recognizes ADIPOQ from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
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ADIPOQ Antibody |
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| CSB-PA828343-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against ADIPOQ. Recognizes ADIPOQ from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
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ADIPOQ Antibody |
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| 1-CSB-PA000806 | Cusabio |
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Description: A polyclonal antibody against ADIPOQ. Recognizes ADIPOQ from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/10000 |
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ADIPOQ Antibody |
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| 1-CSB-PA000014 | Cusabio |
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Description: A polyclonal antibody against ADIPOQ. Recognizes ADIPOQ from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/5000 |
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 Adipoq Antibody |
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| 1-CSB-PA07946A0Rb | Cusabio |
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Description: A polyclonal antibody against Adipoq. Recognizes Adipoq from Mouse. This antibody is Unconjugated. Tested in the following application: ELISA |
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ADIPOQ Antibody |
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| 1-CSB-PA07956A0Rb | Cusabio |
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Description: A polyclonal antibody against ADIPOQ. Recognizes ADIPOQ from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200 |
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ADIPOQ Antibody |
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| 1-CSB-PA07957A0Rb | Cusabio |
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Description: A polyclonal antibody against ADIPOQ. Recognizes ADIPOQ from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200 |
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ADIPOQ Antibody |
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| 1-CSB-PA618019YA01HU | Cusabio |
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Description: A polyclonal antibody against ADIPOQ. Recognizes ADIPOQ from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200 |
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ADIPOQ Antibody |
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| 1-CSB-PA661101LA01BO | Cusabio |
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Description: A polyclonal antibody against ADIPOQ. Recognizes ADIPOQ from bovine. This antibody is Unconjugated. Tested in the following application: ELISA |
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ADIPOQ Antibody |
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| 1-CSB-PA661101YA01BO | Cusabio |
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Description: A polyclonal antibody against ADIPOQ. Recognizes ADIPOQ from Bovine. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:1000-1:5000 |
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ADIPOQ Antibody |
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| 1-CSB-PA938581 | Cusabio |
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Description: A polyclonal antibody against ADIPOQ. Recognizes ADIPOQ from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:1000-1:2000, WB:1:200-1:1000 |
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ADIPOQ Antibody |
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| DF7000 | Affbiotech | 200ul | EUR 304 |
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Description: ADIPOQ Antibody detects endogenous levels of total ADIPOQ. |
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ADIPOQ Antibody |
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| 1-CSB-PA293186 | Cusabio |
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Description: A polyclonal antibody against ADIPOQ. Recognizes ADIPOQ from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:15-1:50 |
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ADIPOQ Antibody |
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| 1-CSB-PA149289 | Cusabio |
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Description: A polyclonal antibody against ADIPOQ. Recognizes ADIPOQ from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:1000-1:2000, WB:1:200-1:1000 |
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 ADIPOQ siRNA |
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| 20-abx906866 | Abbexa |
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ADIPOQ siRNA |
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| 20-abx906867 | Abbexa |
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ADIPOQ Antibody |
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| ABD7000 | Lifescience Market | 100 ug | EUR 438 |
ADIPOQ antibody |
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| PAab09814 | Lifescience Market | 100 ug | EUR 386 |
 (pORF)) ADIPOQ-AS1 ORF Vector (Human) (pORF) |
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| ORF015332 | ABM | 1.0 ug DNA | Ask for price |
 (CMV) (pLenti-GIII-CMV)) ADIPOQ Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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| LV700825 | ABM | 1.0 ug DNA | EUR 514 |
 (UbC) (pLenti-GIII-UbC)) ADIPOQ Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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| LV700829 | ABM | 1.0 ug DNA | EUR 514 |
 (EF1a) (pLenti-GIII-EF1a)) ADIPOQ Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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| LV700830 | ABM | 1.0 ug DNA | EUR 514 |
 Human ADIPOQ ELISA Kit |
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| DEIA215 | Creative Diagnostics | 96T | EUR 850 |
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Description: This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). It is developed for quantitative measurement of Human ADIPOQ in serum, plasma and other biological fluids. |
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Human ADIPOQ ELISA Kit |
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| ELA-E0605h | Lifescience Market | 96 Tests | EUR 824 |
 Human ADIPOQ shRNA Plasmid |
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| 20-abx956206 | Abbexa |
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Human ADIPOQ ELISA Kit |
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| STJ150096 | St John's Laboratory | 1 kit | EUR 412 |
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Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ADP/Acrp30 in human serum, plasma and other biological fluids |
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 (pORF)) Adipoq ORF Vector (Rat) (pORF) |
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| ORF063117 | ABM | 1.0 ug DNA | EUR 506 |
 (pORF)) Adipoq ORF Vector (Mouse) (pORF) |
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| ORF038176 | ABM | 1.0 ug DNA | EUR 95 |
 (pPB-His-MBP)) ADIPOQ Protein Vector (Human) (pPB-His-MBP) |
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| PV320122 | ABM | 500 ng | EUR 481 |
 (pPB-His-GST)) ADIPOQ Protein Vector (Human) (pPB-His-GST) |
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| PV320123 | ABM | 500 ng | EUR 481 |
 (pPB-C-His)) ADIPOQ Protein Vector (Human) (pPB-C-His) |
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| PV048649 | ABM | 500 ng | EUR 481 |
 (pPB-N-His)) ADIPOQ Protein Vector (Human) (pPB-N-His) |
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| PV048650 | ABM | 500 ng | EUR 481 |
 (pPM-C-HA)) ADIPOQ Protein Vector (Human) (pPM-C-HA) |
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| PV048651 | ABM | 500 ng | EUR 481 |
 (pPM-C-His)) ADIPOQ Protein Vector (Human) (pPM-C-His) |
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| PV048652 | ABM | 500 ng | EUR 481 |
) Bovine Adiponectin (ADIPOQ) |
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| 1-CSB-EP661101BO | Cusabio |
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Description: Recombinant Bovine Adiponectin(ADIPOQ) expressed in E.coli |
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) Mouse Adiponectin (Adipoq) |
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| 1-CSB-EP723362MO | Cusabio |
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Description: Recombinant Mouse Adiponectin(Adipoq) expressed in E.coli |
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Mouse Adiponectin (Adipoq) |
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| 1-CSB-RP079474m | Cusabio |
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Description: Recombinant Mouse Adiponectin(Adipoq),partial expressed in E.coli |
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 ADIPOQ Blocking Peptide |
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| DF7000-BP | Affbiotech | 1mg | EUR 195 |
Bovine Adiponectin (ADIPOQ) |
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| 1-CSB-YP661101BO | Cusabio |
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Description: Recombinant Bovine Adiponectin(ADIPOQ) expressed in Yeast |
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 Antibody) Adiponectin (ADIPOQ) Antibody |
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| abx147913-100ug | Abbexa | 100 ug | EUR 439 |
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Adiponectin (ADIPOQ) Antibody |
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| 20-abx141288 | Abbexa |
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Adiponectin (ADIPOQ) Antibody |
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| abx239837-100ug | Abbexa | 100 ug | EUR 481 |
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 ADIPOQ Conjugated Antibody |
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| C31141 | SAB | 100ul | EUR 397 |
ADIPOQ Conjugated Antibody |
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| C38421 | SAB | 100ul | EUR 397 |
Adiponectin (ADIPOQ) Antibody |
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| abx224369-100ug | Abbexa | 100 ug | EUR 411 |
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Adiponectin (ADIPOQ) Antibody |
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| abx224470-100ug | Abbexa | 100 ug | EUR 411 |
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Adiponectin (ADIPOQ) Antibody |
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| abx230170-100ug | Abbexa | 100 ug | EUR 481 |
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 ADIPOQ cloning plasmid |
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| CSB-CL618019HU-10ug | Cusabio | 10ug | EUR 233 |
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Description: A cloning plasmid for the ADIPOQ gene. |
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 Adipoq Polyclonal Antibody |
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| A56646 | EpiGentek | 100 µg | EUR 570.55 |
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Description: kits suitable for this type of research |
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 ADIPOQ Polyclonal Antibody |
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| A53605 | EpiGentek | 100 µg | EUR 570.55 |
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Description: Ask the seller for details |
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ADIPOQ Polyclonal Antibody |
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| A54069 | EpiGentek | 100 µg | EUR 570.55 |
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Description: kits suitable for this type of research |
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ADIPOQ Polyclonal Antibody |
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| A54866 | EpiGentek | 100 µg | EUR 570.55 |
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Description: Ask the seller for details |
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ADIPOQ Polyclonal Antibody |
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| A55046 | EpiGentek | 100 µg | EUR 570.55 |
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Description: The best epigenetics products |
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ADIPOQ Polyclonal Antibody |
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| A55747 | EpiGentek | 100 µg | EUR 570.55 |
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Description: The best epigenetics products |
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 ADIPOQ Rabbit pAb |
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| A2543-100ul | Abclonal | 100 ul | EUR 308 |
ADIPOQ Rabbit pAb |
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| A2543-200ul | Abclonal | 200 ul | EUR 459 |
ADIPOQ Rabbit pAb |
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| A2543-20ul | Abclonal | 20 ul | EUR 183 |
ADIPOQ Rabbit pAb |
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| A2543-50ul | Abclonal | 50 ul | EUR 223 |
Adiponectin (ADIPOQ) Antibody |
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| 20-abx001981 | Abbexa |
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 anti- ADIPOQ antibody |
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| FNab09814 | FN Test | 100µg | EUR 548.75 |
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Description: Antibody raised against ADIPOQ |
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anti- ADIPOQ antibody |
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| FNab00170 | FN Test | 100µg | EUR 505.25 |
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Description: Antibody raised against ADIPOQ |
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anti- ADIPOQ antibody |
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| LSMab09814 | Lifescience Market | 100 ug | EUR 386 |
 Anti-ADIPOQ antibody |
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| STJ400002 | St John's Laboratory | 1 mg | EUR 446 |
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Description: Adiponectin is a protein hormone that modulates a number of metabolic processes that may lead to type 2 diabetes, obesity and atherosclerosis. It has been studied as a prognostic and diagnostic marker of diabetes and cardiovascular diseases. |
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Anti-ADIPOQ antibody |
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| STJ400003 | St John's Laboratory | 1 mg | EUR 446 |
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Description: Adiponectin is a protein hormone that modulates a number of metabolic processes that may lead to type 2 diabetes, obesity and atherosclerosis. It has been studied as a prognostic and diagnostic marker of diabetes and cardiovascular diseases. |
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Anti-ADIPOQ antibody |
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| STJ400004 | St John's Laboratory | 1 mg | EUR 446 |
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Description: Adiponectin is a protein hormone that modulates a number of metabolic processes that may lead to type 2 diabetes, obesity and atherosclerosis. It has been studied as a prognostic and diagnostic marker of diabetes and cardiovascular diseases. |
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Anti-ADIPOQ antibody |
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| STJ22521 | St John's Laboratory | 100 µl | EUR 277 |
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Description: This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
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 Human ADIPOQ/ Adiponectin ELISA Kit |
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| E0067Hu | Sunlong | 1 Kit | EUR 537 |
 ELISA Kit) Human Adiponectin (ADIPOQ) ELISA Kit |
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| abx050005-96tests | Abbexa | 96 tests | EUR 668 |
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Human Adiponectin (ADIPOQ) ELISA Kit |
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| abx051753-96tests | Abbexa | 96 tests | EUR 668 |
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Human Adiponectin (ADIPOQ) ELISA Kit |
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| 20-abx150569 | Abbexa |
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Human Adiponectin (ADIPOQ) ELISA Kit |
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| abx251970-96tests | Abbexa | 96 tests | EUR 637 |
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 Human Adiponectin, ADIPOQ ELISA KIT |
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| ELI-02179h | Lifescience Market | 96 Tests | EUR 824 |
Human Adiponectin (ADIPOQ) ELISA Kit |
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| abx570358-96tests | Abbexa | 96 tests | EUR 637 |
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 Protein (OVA)) Human Adiponectin (ADIPOQ) Protein (OVA) |
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| 20-abx651034 | Abbexa |
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 (OKAN04663)) ADIPOQ ELISA Kit (Human) (OKAN04663) |
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| OKAN04663 | Aviva Systems Biology | 96 Wells | EUR 792 |
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Description: Description of target: This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 7 pg/mL |
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 (OKBB01509)) ADIPOQ ELISA Kit (Human) (OKBB01509) |
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| OKBB01509 | Aviva Systems Biology | 96 Wells | EUR 648 |
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Description: Description of target: Adiponectin(ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226(19-244) amino acids with the molecular mass of 36KDa after glycosylation.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: <60pg/ml |
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 (OKCD00248)) ADIPOQ ELISA Kit (Human) (OKCD00248) |
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| OKCD00248 | Aviva Systems Biology | 96 Wells | EUR 675 |
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Description: Description of target: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.1 Publication
<p>Manually curated information for which there is published experimental evidence.</p>
<p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.9"The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity."_x005F_x005F_x000D_Yamauchi T., Kamon J., Waki H., Terauchi Y., Kubota N., Hara K., Mori Y., Ide T., Murakami K., Tsuboyama-Kasaoka N., Ezaki O., Akanuma Y., Gavrilova O., Vinson C., Reitman M.L., Kagechika H., Shudo K., Yoda M. , Nakano Y., Tobe K., Nagai R., Kimura S., Tomita M., Froguel P., Kadowaki T._x005F_x005F_x000D_Nat. Med. 7:941-946(2001) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.069 ng/mL |
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Environment friendly manufacturing of recombinant adeno-associated viral vector, serotype DJ/8, carrying the GFP gene.
The aim of this examine was to determine an environment friendly technique for the preparation of an adeno-associated viral (AAV), serotype DJ/8, carrying the GFP gene (AAV-DJ/8-GFP). We in contrast the yields of AAV-DJ/Eight vector, which have been produced by three completely different mixture strategies, consisting of two plasmid DNA transfection strategies (lipofectamine and calcium phosphate co-precipitation; CaPi) and two virus DNA purification strategies (iodixanol and cesium chloride; CsCl). The outcomes confirmed that the very best yield of AAV-DJ/8-GFP vector was completed with the mixture technique of lipofectamine transfection and iodixanol purification.
The viral protein expression ranges and the transduction efficacy in HEK293 and CHO cells weren’t completely different amongst 4 completely different mixture strategies for AAV-DJ/8-GFP vectors. We confirmed that the AAV-DJ/8-GFP vector might transduce to human and murine hepatocyte-derived cell traces. These outcomes present that AAV-DJ/8-GFP, purified by the mixture of lipofectamine and iodixanol, produces an environment friendly yield with out altering the traits of protein expression and AAV gene transduction
A CGMMV genome-replicon vector with partial sequences of coat protein gene effectively expresses GFP in Nicotiana benthamiana.
A extremely infectious clone of Cucumber inexperienced mottle mosaic virus (CGMMV), a cucurbit-infecting tobamovirus was utilized for designing of gene expression vectors. Two variations of vector have been examined for his or her efficacy in expressing the inexperienced fluorescent protein (GFP) in Nicotiana benthamiana. When the GFP gene was inserted on the cease codon of coat protein (CP) gene of the CGMMV genome with none read-through codon, systemic expression of GFP, in addition to virion formation and systemic signs expression have been obtained in N. benthamiana.
The qRT-PCR evaluation confirmed 23 fold enhance of GFP over actin at 10days publish inoculation (dpi), which elevated to 45 fold at 14dpi and thereafter the GFP expression was considerably declined. Additional, we present that when the a lot of the CP sequence is deleted retaining solely the primary 105 nucleotides, the shortened vector containing GFP in body of authentic CP open studying body (ORF) resulted in 234 fold enhance of GFP expression over actin at 5dpi in N. benthamiana with out the formation of virions and illness signs. Our examine demonstrated {that a} easy manipulation of CP gene within the CGMMV genome whereas preserving the translational body of CP resulted in growing a virus-free, fast and environment friendly international protein expression system within the plant. The CGMMV primarily based vectors developed on this examine could also be probably helpful for the manufacturing of edible vaccines in cucurbits
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