Double-labeled lentiviral vector containing CK8 interfering RNA

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gfpvector

Building of a GFP/Puro double-labeled lentiviral vector containing CK8 interfering RNA and its impact on cell apoptosis in vitro

OBJECTIVE
To assemble a GFP/Puro double-labeled lentiviral expression vector for CK8 silencing and assess the consequences of CK8 silencing on cell apoptosis.
METHODS
The siRNA sequences of CK8 have been inserted into the lentiviral expression vector GV248 and transfected into 293T cells with the packaging plasmids PMD and SPA. The lentivirus was collected at 24 and 36 h post-transfection. Circulate cytometry was used to detect the virus titer and the optimistic cells have been chosen with puromycin. The knockdown of CK8 was examined by Western blotting. The impact of CK8 down-regulation on cell apoptosis induced by cisplatin was detected with Annexin V/PI staining.
CONCLUSIONS
We efficiently constructed CK8 interference lentiviral vector and obtained a steady cell line with CK8 knock-down that was delicate to cisplatin-induced apoptosis
gfpvector

gfpvector

SPOCK3 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector
TL309139 5 µg/vial Ask for price

SPOCK2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector
TL309140 5 µg/vial Ask for price

SPOCK3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)
LV647761 1.0 ug DNA
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SPOCK3 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)
LV647765 1.0 ug DNA
EUR 818.4

SPOCK3 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)
LV647766 1.0 ug DNA
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SPOCK1 ORF Vector (Human) (pORF)
ORF009985 1.0 ug DNA
EUR 114

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SPOCK3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
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SPOCK1 Protein Vector (Human) (pPM-C-HA)
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Spock2 - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector
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Spock3 - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector
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SPOCK1 Protein Vector (Human) (pPB-C-His)
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SPOCK1 Protein Vector (Human) (pPB-N-His)
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Spock3 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector
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Spock2 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector
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SPOCK1 sgRNA CRISPR Lentivector set (Human)
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SPOCK1 Protein Vector (Mouse) (pPM-C-His)
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SPOCK1 Protein Vector (Mouse) (pPB-N-His)
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SPOCK1 Protein Vector (Mouse) (pPM-C-His)
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SPOCK1 Protein Vector (Mouse) (pPM-C-His)
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SPOCK1 Protein Vector (Mouse) (pPB-C-His)
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SPOCK1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector
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Human SPOCK1 siRNA
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SPOCK1 siRNA (Human)
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SPOCK1 ELISA Kit (Human) (OKBB01262)
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Lenti-ORF clone of SPOCK1 (mGFP-tagged)-Human sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1 (SPOCK1)
RC205296L4 10 µg Ask for price

Spock1 sgRNA CRISPR Lentivector set (Mouse)
K3875701 3 x 1.0 ug
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TRV-GFP: a modified Tobacco rattle virus vector for environment friendly and visualizable evaluation of gene operate.

Virus-induced gene silencing (VIGS) is a great tool for useful characterization of genes in crops. Sadly, the effectivity of an infection by Tobacco rattle virus (TRV) is comparatively low for some non-Solanaceae crops, that are economically necessary, comparable to rose (Rosa sp.).
Right here, to generate a simple traceable TRV vector, a inexperienced fluorescent protein (GFP) gene was tagged to the three’ terminus of the coat protein gene within the authentic TRV2 vector, and the silencing effectivity of the modified TRV-GFP vector was examined in a number of crops, together with Nicotiana benthamiana, Arabidopsis thaliana, rose, strawberry (Fragaria ananassa), and chrysanthemum (Dendranthema grandiflorum).
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When TRV-GFP was used to silence the endogenous phytoene desaturase (PDS) gene in rose cuttings and seedlings, the standard photobleached phenotype was noticed in 75-80% crops which have been recognized as GFP optimistic by UV lamp.
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Taken collectively, this work gives a visualizable and environment friendly instrument to foretell optimistic gene silencing crops, which is effective for analysis into gene operate in crops, particularly for non-Solanaceae plant

A Mason-Pfizer Monkey virus Gag-GFP fusion vector permits visualization of capsid transport in dwell cells and demonstrates a job for microtubules.

Immature capsids of the Betaretrovirus, Mason-Pfizer Monkey virus (M-PMV), are assembled within the pericentriolar area of the cell, and are then transported to the plasma membrane for budding.
Though a number of research, using mutagenesis, biochemistry, and immunofluorescence, have outlined the position of some viral and host cells elements concerned in these processes, they’ve the drawback of inhabitants evaluation, relatively than analyzing particular person capsid motion in actual time.
On this examine, we created an M-PMV vector wherein the improved inexperienced fluorescent protein, eGFP, was fused to the carboxyl-terminus of the M-PMV Gag polyprotein, to create a Gag-GFP fusion that might be visualized in dwell cells.
In an effort to categorical this fusion protein within the context of an M-PMV proviral spine, it was essential to codon-optimize gag, optimize the Kozak sequence previous the initiating methionine, and mutate an inside methionine codon to 1 for alanine (M100A) to forestall inside initiation of translation.
Co-expression of this pSARM-Gag-GFP-M100A vector with a WT M-PMV provirus resulted in environment friendly meeting and launch of capsids.
Outcomes from fixed-cell immunofluorescence and pulse-chase analyses of untamed sort and mutant Gag-GFP constructs demonstrated comparable intracellular localization and launch of capsids to untagged counterparts. Actual-time, live-cell visualization and evaluation of the GFP-tagged capsids supplied robust proof for a job for microtubules within the intracellular transport of M-PMV capsids. Thus, this M-PMV Gag-GFP vector is a great tool for figuring out novel virus-cell interactions concerned in intracellular M-PMV capsid transport in a dynamic, real-time system

Flat Replaceable Tips 25 mm Diameter Flat Titanium Tip
0-120-0018 25 mm
EUR 100
Description: use with corresponding Tapped Tips and Tapped Extender Tips

Titanium Cup Tip, 250 ml
0-120-0019 250 ml
EUR 1377
Description: includes Interface Washers

Microtube Tray, 8 Position (for 250 ml Cup Tip)
0-120-0021 each
EUR 113
Description: includes Interface Washers

Continuous Flow Chamber
0-120-0026 each
EUR 774
Description: includes Interface Washers

Interface Washers 1.5” Diameter, 5/Pkg
0-120-003 1.5'' Diameter
EUR 31

13 mm Diameter Solid Titanium Extender Tip
0-120-0032 13 mm
EUR 301

13 mm Diameter Tapped Titanium Extender Tip
0-120-0033 13 mm
EUR 362

19 mm Diameter Solid Titanium Extender Tip
0-120-0034 19 mm
EUR 315

19 mm Diameter Tapped Titanium Extender Tip
0-120-0035 19 mm
EUR 375

25 mm Diameter Solid Titanium Extender Tip
0-120-0036 25 mm
EUR 328

25 mm Diameter Tapped Titanium Extender Tip
0-120-0037 25 mm
EUR 389

KoldPod, 1.5ml Micro Tube
0-120-0038 1.5 ml
EUR 152
Description: Thermo conductive tube pods

KoldPod, 15ml Conical Tube
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EUR 275
Description: Thermo conductive tube pods

KoldPod, 50ml Conical Tube
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Description: Thermo conductive tube pods

Model 150 V/T Ultrasonic Homogenizer
0-121-0002 210-240V/50-60Hz
EUR 2920
Description: Delivers up to 150 Watts of ultrasonic power to the Titanium Tip. The Timer and Duty Cycle function increase preciosion in sample processing processing.

Model 300 V/T Ultrasonic Homogenizer
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EUR 3520
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip. The Timer and Duty Cycle function increase preciosion in sample.

SONABOZ Sound Abating Chamber
0-125-0001 each
EUR 1020
Description: Reduces cavitational sound emitted during processing.

Model 3000 Ultrasonic Homogenizer
0-127-0002 210-240V/50-60Hz
EUR 4120
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip and includes an intergrated Sound Abating Chmaber to reduce cavitational sound emitted during processing. The Timer and Duty Cycle function increase preciosion in sample.

Model 3000MP Ultrasonic Homogenizer
0-128-0002 210-240V/50-60Hz
EUR 4720
Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip with preciosion control from a microprocessor and a graphical user interface displayed on a large (145 mm) LCD display. The integrated Sound Abating Chamber reduces cavitational sound emitted during processing.

OMNICON® Zone Reader, 210-240V/50-60Hz
0-131-0002 210-240V/50-60Hz
EUR 35200
Description: Designed to Perform multi-plate Assays on round 90/100mm Petri Dishes. The integrated LED illumination system provides transmitted light for brightfield and darkfield illumination of transparent media.

OMNI-Noculator Peni Cylinder Filler, 210-240V/50-60Hz
0-134-0002 210-240V/50-60Hz
EUR 32200
Description: A robotic liquid handling system designed to dispense Peni Cylinders and fill Peni Cylinders with the corresponding antibiotic liquid sample.

Peni Cylinder Dispenser with Manual Hopper
0-144-0002 each
EUR 5406
Description: Dispenser can be configured to dispense 4 or 6 Peni Cylinders onto a petri dish.

Peni Cylinder Dispenser with Motorized Hopper, 100-240V/50-60Hz
0-144-0003 100-240V/50-60Hz
EUR 6254
Description: The motorized hopper can be configured to dispense 4 or 6 Peni Cylinders onto a petri Dispenser can be disassembled for disinfection.

Stainless Steel Peni Cylinder with Flat Face
0-144-0005 6mm I.D. x 8mm O.D. x 10mm Long
EUR 399
Description: sold in packages of 100 pieces

Stainless Steel Peni Cylinder with Chamfered Face
0-144-0006 6mm I.D. x 8mm O.D. x 10mm Long
EUR 412
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Custom development of ELISAs for other species or antibody isotypes not listed in the catalog. Custom testing of samples for IgG/IgM/IgA or total (IgG+IgM+IgA)
000-CUS Custom
EUR 602

Alpha-Bungarotoxin, CF®405S, 500 ug
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SARS-CoV-2 Indicator Cell Line for RNA Replication - GFP Reporter only
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Description: SARS-CoV-2 GFP reporter cell line using HEK293T (ACE2/TMPRSS2) cells

Alpha-Bungarotoxin, 1 mg
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Alpha-Bungarotoxin, 1 mg
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Fluorescein-Alpha-Bungarotoxin, 500 ug
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Fluorescein-Alpha-Bungarotoxin, 500 ug
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Tetramethylrhodamine-Alpha-Bungarotoxin, 500 ug
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Tetramethylrhodamine-Alpha-Bungarotoxin, 500 ug
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Fluorescein-alpha-bungarotoxin, 10x50ug
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Tetramethylrhodamine-A-Bungarotoxin, 10x50 ug
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Tetramethylrhodamine-A-Bungarotoxin, 10x50 ug
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Sulforhodamine 101-Alpha-Bungarotoxin, 500 ug
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Sulforhodamine 101-Alpha-Bungarotoxin, 500 ug
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Sulforhodamine 101-Alpha-Bungarotoxin, 50 ug
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Sulforhodamine 101-Alpha-Bungarotoxin, 50 ug
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Alpha-Bungarotoxin, CF®555, 500 ug
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  • Ask for price
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Alpha-Bungarotoxin, CF®555, 500 ug
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Alpha-Bungarotoxin, CF®555 100ug
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Acrylamide, Chemzymes Ultra Pure®
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Description: 79-06-1

Acrylamide, Chemzymes Ultra Pure®
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Biotin-cAMP, 1 mg
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Description: N/A

Biotin-cAMP, 1 mg
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Biotin-cAMP, 50 ug
00020-1-1 EA
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Biotin-cGMP, 1 mg
00021 1MG
EUR 331
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Biotin-cGMP, 1 mg
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Biotin-cGMP, 20x50 ug
00021-1-1 EA
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Cyanine 644-cAMP, 1 mg
00022 1MG
EUR 496
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Cyanine 644-cAMP, 1 mg
00022-1 20ST
EUR 496
Description: N/A

Cyanine 644-cAMP, 20x50 ug
00022-1-1 EA
EUR 647

Fluorescein Methotrexate, Triammonium Salt, 1 mg
00023 1MG
EUR 285
Description: N/A

Fluorescein Methotrexate, Triammonium Salt, 1 mg
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EUR 285

Staurosporine
00025 100uG
EUR 100
Description: N/A

Staurosporine
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EUR 100

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00026 500uG
EUR 527
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Alpha-Bungarotoxin, CF®543, 500 ug
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Alpha-Bungarotoxin, CF®543, 100 ug
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