Double-labeled lentiviral vector containing CK8 interfering RNA

gfpvector

Building of a GFP/Puro double-labeled lentiviral vector containing CK8 interfering RNA and its impact on cell apoptosis in vitro

OBJECTIVE
To assemble a GFP/Puro double-labeled lentiviral expression vector for CK8 silencing and assess the consequences of CK8 silencing on cell apoptosis.
METHODS
The siRNA sequences of CK8 have been inserted into the lentiviral expression vector GV248 and transfected into 293T cells with the packaging plasmids PMD and SPA. The lentivirus was collected at 24 and 36 h post-transfection. Circulate cytometry was used to detect the virus titer and the optimistic cells have been chosen with puromycin. The knockdown of CK8 was examined by Western blotting. The impact of CK8 down-regulation on cell apoptosis induced by cisplatin was detected with Annexin V/PI staining.
CONCLUSIONS
We efficiently constructed CK8 interference lentiviral vector and obtained a steady cell line with CK8 knock-down that was delicate to cisplatin-induced apoptosis
gfpvector

gfpvector

anti-SPOCK1

YF-PA14763 50 ug
EUR 363
Description: Mouse polyclonal to SPOCK1

SPOCK1 ELISA KIT|Human

EF003197 96 Tests
EUR 689

Human SPOCK1 shRNA Plasmid

20-abx954565
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

SPOCK1 Recombinant Protein (Human)

RP029953 100 ug Ask for price

Spock1 ORF Vector (Mouse) (pORF)

ORF058387 1.0 ug DNA
EUR 506

Spock1 ORF Vector (Mouse) (pORF)

ORF058388 1.0 ug DNA
EUR 506

Spock1 ORF Vector (Mouse) (pORF)

ORF058389 1.0 ug DNA
EUR 506

Spock1 ORF Vector (Mouse) (pORF)

ORF058390 1.0 ug DNA
EUR 506

Spock1 ORF Vector (Mouse) (pORF)

ORF058391 1.0 ug DNA
EUR 506

Anti-SPOCK1 antibody

PAab08186 100 ug
EUR 386

SPOCK1 Protein Vector (Human) (pPB-C-His)

PV039937 500 ng
EUR 329

SPOCK1 Protein Vector (Human) (pPB-N-His)

PV039938 500 ng
EUR 329

SPOCK1 Protein Vector (Human) (pPM-C-HA)

PV039939 500 ng
EUR 329

SPOCK1 Protein Vector (Human) (pPM-C-His)

PV039940 500 ng
EUR 329

SPOCK1 ELISA Kit (Human) (OKBB01262)

OKBB01262 96 Wells
EUR 505
Description: Description of target: Testican-1 is a protein that in humans is encoded by the SPOCK1 gene. It is mapped to 5q31.2. This gene encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be related to protease inhibition. ;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml

SPOCK1 Antibody, HRP conjugated

1-CSB-PA730785LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against SPOCK1. Recognizes SPOCK1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

SPOCK1 Antibody, FITC conjugated

1-CSB-PA730785LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against SPOCK1. Recognizes SPOCK1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

SPOCK1 Antibody, Biotin conjugated

1-CSB-PA730785LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against SPOCK1. Recognizes SPOCK1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

SPOCK1 Recombinant Protein (Mouse)

RP175157 100 ug Ask for price

SPOCK1 Recombinant Protein (Mouse)

RP175160 100 ug Ask for price

SPOCK1 Recombinant Protein (Mouse)

RP175163 100 ug Ask for price

SPOCK1 Recombinant Protein (Mouse)

RP175166 100 ug Ask for price

SPOCK1 Recombinant Protein (Mouse)

RP175169 100 ug Ask for price

Human Testican- 1, SPOCK1 ELISA KIT

ELI-41909h 96 Tests
EUR 824

SPOCK1 sgRNA CRISPR Lentivector set (Human)

K2278001 3 x 1.0 ug
EUR 339

Recombinant Human Testican 1/SPOCK1 Protein

RP01051 5 μg
EUR 136

SPOCK1 Protein Vector (Mouse) (pPB-C-His)

PV233546 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPB-N-His)

PV233547 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPM-C-HA)

PV233548 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPM-C-His)

PV233549 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPB-C-His)

PV233550 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPB-N-His)

PV233551 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPM-C-HA)

PV233552 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPM-C-His)

PV233553 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPB-C-His)

PV233554 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPB-N-His)

PV233555 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPM-C-HA)

PV233556 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPM-C-His)

PV233557 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPB-C-His)

PV233558 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPB-N-His)

PV233559 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPM-C-HA)

PV233560 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPM-C-His)

PV233561 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPB-C-His)

PV233562 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPB-N-His)

PV233563 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPM-C-HA)

PV233564 500 ng
EUR 603

SPOCK1 Protein Vector (Mouse) (pPM-C-His)

PV233565 500 ng
EUR 603

Polyclonal SPOCK1 Antibody(N-term)

APR13512G 0.1ml
EUR 528
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human SPOCK1 (N-term). This antibody is tested and proven to work in the following applications:

Human Testican 1 / TIC1 (SPOCK1) ELISA Kit

20-abx258185
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

Human SPOCK1/Testican-1 PicoKine ELISA Kit

EK1848 96 wells
EUR 425
Description: For quantitative detection of human SPOCK1/Testican-1 in cell culture supernates, serum and plasma (heparin, EDTA).

Human Testican 1 / TIC1 (SPOCK1) CLIA Kit

20-abx496043
  • EUR 8569.00
  • EUR 4560.00
  • EUR 1052.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Please enquire.

SPOCK1 sgRNA CRISPR Lentivector (Human) (Target 1)

K2278002 1.0 ug DNA
EUR 154

SPOCK1 sgRNA CRISPR Lentivector (Human) (Target 2)

K2278003 1.0 ug DNA
EUR 154

SPOCK1 sgRNA CRISPR Lentivector (Human) (Target 3)

K2278004 1.0 ug DNA
EUR 154

ELISA kit for Human Testican-1 (SPOCK1)

KTE60335-48T 48T
EUR 332
  • TIC1 encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be relat
  • Show more
Description: Quantitative sandwich ELISA for measuring Human Testican-1 (SPOCK1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Testican-1 (SPOCK1)

KTE60335-5platesof96wells 5 plates of 96 wells
EUR 2115
  • TIC1 encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be relat
  • Show more
Description: Quantitative sandwich ELISA for measuring Human Testican-1 (SPOCK1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Testican-1 (SPOCK1)

KTE60335-96T 96T
EUR 539
  • TIC1 encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be relat
  • Show more
Description: Quantitative sandwich ELISA for measuring Human Testican-1 (SPOCK1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Mouse Testican- 1, Spock1 ELISA KIT

ELI-41910m 96 Tests
EUR 865

Spock1 sgRNA CRISPR Lentivector set (Mouse)

K3875701 3 x 1.0 ug
EUR 339

Spock1 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3875702 1.0 ug DNA
EUR 154

Spock1 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3875703 1.0 ug DNA
EUR 154

Spock1 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3875704 1.0 ug DNA
EUR 154

ELISA kit for Mouse Testican-1 (SPOCK1)

KTE70235-48T 48T
EUR 332
  • TIC1 encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be relat
  • Show more
Description: Quantitative sandwich ELISA for measuring Mouse Testican-1 (SPOCK1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Testican-1 (SPOCK1)

KTE70235-5platesof96wells 5 plates of 96 wells
EUR 2115
  • TIC1 encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be relat
  • Show more
Description: Quantitative sandwich ELISA for measuring Mouse Testican-1 (SPOCK1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Testican-1 (SPOCK1)

KTE70235-96T 96T
EUR 539
  • TIC1 encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be relat
  • Show more
Description: Quantitative sandwich ELISA for measuring Mouse Testican-1 (SPOCK1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Spock1 3'UTR Luciferase Stable Cell Line

TU119621 1.0 ml Ask for price

Spock1 3'UTR GFP Stable Cell Line

TU169621 1.0 ml Ask for price

Spock1 3'UTR Luciferase Stable Cell Line

TU221107 1.0 ml Ask for price

SPOCK1 3'UTR GFP Stable Cell Line

TU074515 1.0 ml
EUR 2333

Spock1 3'UTR GFP Stable Cell Line

TU271107 1.0 ml Ask for price

SPOCK1 3'UTR Luciferase Stable Cell Line

TU024515 1.0 ml
EUR 2333

SPOCK1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K2278005 3 x 1.0 ug
EUR 376

SPOCK1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K2278006 1.0 ug DNA
EUR 167

SPOCK1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K2278007 1.0 ug DNA
EUR 167

SPOCK1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K2278008 1.0 ug DNA
EUR 167

Spock1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K3875705 3 x 1.0 ug
EUR 376

Human SPARC/Osteonectin, Cwcv And Kazal Like Domains Proteoglycan 1 (SPOCK1) ELISA Kit

abx383426-96tests 96 tests
EUR 911
  • Shipped within 5-12 working days.

SPARC/Osteonectin, Cwcv And Kazal Like Domains Proteoglycan 1 (SPOCK1) Antibody

abx145667-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.

SPARC/Osteonectin, Cwcv And Kazal Like Domains Proteoglycan 1 (SPOCK1) Antibody

abx238186-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.

SPARC/Osteonectin, Cwcv And Kazal Like Domains Proteoglycan 1 (SPOCK1) Antibody

20-abx318007
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Spock1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K3875706 1.0 ug DNA
EUR 167

TRV-GFP: a modified Tobacco rattle virus vector for environment friendly and visualizable evaluation of gene operate.

Virus-induced gene silencing (VIGS) is a great tool for useful characterization of genes in crops. Sadly, the effectivity of an infection by Tobacco rattle virus (TRV) is comparatively low for some non-Solanaceae crops, that are economically necessary, comparable to rose (Rosa sp.).
Right here, to generate a simple traceable TRV vector, a inexperienced fluorescent protein (GFP) gene was tagged to the three’ terminus of the coat protein gene within the authentic TRV2 vector, and the silencing effectivity of the modified TRV-GFP vector was examined in a number of crops, together with Nicotiana benthamiana, Arabidopsis thaliana, rose, strawberry (Fragaria ananassa), and chrysanthemum (Dendranthema grandiflorum).
The outcomes confirmed that the effectivity of an infection by TRV-GFP was equal to that of the unique TRV vector in every examined plant. Unfold of the modified TRV virus was simple to observe by utilizing fluorescent microscopy and a hand-held UV lamp.
When TRV-GFP was used to silence the endogenous phytoene desaturase (PDS) gene in rose cuttings and seedlings, the standard photobleached phenotype was noticed in 75-80% crops which have been recognized as GFP optimistic by UV lamp.
As well as, the abundance of GFP protein, which represented the focus of TRV virus, was proved to correlate negatively with the extent of the PDS gene, suggesting that GFP might be used as an indicator of the diploma of silencing of a goal gene.
Taken collectively, this work gives a visualizable and environment friendly instrument to foretell optimistic gene silencing crops, which is effective for analysis into gene operate in crops, particularly for non-Solanaceae plant

A Mason-Pfizer Monkey virus Gag-GFP fusion vector permits visualization of capsid transport in dwell cells and demonstrates a job for microtubules.

Immature capsids of the Betaretrovirus, Mason-Pfizer Monkey virus (M-PMV), are assembled within the pericentriolar area of the cell, and are then transported to the plasma membrane for budding.
Though a number of research, using mutagenesis, biochemistry, and immunofluorescence, have outlined the position of some viral and host cells elements concerned in these processes, they’ve the drawback of inhabitants evaluation, relatively than analyzing particular person capsid motion in actual time.
On this examine, we created an M-PMV vector wherein the improved inexperienced fluorescent protein, eGFP, was fused to the carboxyl-terminus of the M-PMV Gag polyprotein, to create a Gag-GFP fusion that might be visualized in dwell cells.
In an effort to categorical this fusion protein within the context of an M-PMV proviral spine, it was essential to codon-optimize gag, optimize the Kozak sequence previous the initiating methionine, and mutate an inside methionine codon to 1 for alanine (M100A) to forestall inside initiation of translation.
Co-expression of this pSARM-Gag-GFP-M100A vector with a WT M-PMV provirus resulted in environment friendly meeting and launch of capsids.
Outcomes from fixed-cell immunofluorescence and pulse-chase analyses of untamed sort and mutant Gag-GFP constructs demonstrated comparable intracellular localization and launch of capsids to untagged counterparts. Actual-time, live-cell visualization and evaluation of the GFP-tagged capsids supplied robust proof for a job for microtubules within the intracellular transport of M-PMV capsids. Thus, this M-PMV Gag-GFP vector is a great tool for figuring out novel virus-cell interactions concerned in intracellular M-PMV capsid transport in a dynamic, real-time system
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