Double-labeled lentiviral vector containing CK8 interfering RNA

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gfpvector

Building of a GFP/Puro double-labeled lentiviral vector containing CK8 interfering RNA and its impact on cell apoptosis in vitro

OBJECTIVE
To assemble a GFP/Puro double-labeled lentiviral expression vector for CK8 silencing and assess the consequences of CK8 silencing on cell apoptosis.
METHODS
The siRNA sequences of CK8 have been inserted into the lentiviral expression vector GV248 and transfected into 293T cells with the packaging plasmids PMD and SPA. The lentivirus was collected at 24 and 36 h post-transfection. Circulate cytometry was used to detect the virus titer and the optimistic cells have been chosen with puromycin. The knockdown of CK8 was examined by Western blotting. The impact of CK8 down-regulation on cell apoptosis induced by cisplatin was detected with Annexin V/PI staining.
CONCLUSIONS
We efficiently constructed CK8 interference lentiviral vector and obtained a steady cell line with CK8 knock-down that was delicate to cisplatin-induced apoptosis
gfpvector

gfpvector

SPOCK3 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector
TL309139 5 µg/vial Ask for price

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SPOCK3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)
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SPOCK3 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)
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SPOCK3 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)
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SPOCK1 ORF Vector (Human) (pORF)
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Lenti-ORF clone of SPOCK1 (mGFP-tagged)-Human sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1 (SPOCK1)
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TRV-GFP: a modified Tobacco rattle virus vector for environment friendly and visualizable evaluation of gene operate.

Virus-induced gene silencing (VIGS) is a great tool for useful characterization of genes in crops. Sadly, the effectivity of an infection by Tobacco rattle virus (TRV) is comparatively low for some non-Solanaceae crops, that are economically necessary, comparable to rose (Rosa sp.).
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A Mason-Pfizer Monkey virus Gag-GFP fusion vector permits visualization of capsid transport in dwell cells and demonstrates a job for microtubules.

Immature capsids of the Betaretrovirus, Mason-Pfizer Monkey virus (M-PMV), are assembled within the pericentriolar area of the cell, and are then transported to the plasma membrane for budding.
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In an effort to categorical this fusion protein within the context of an M-PMV proviral spine, it was essential to codon-optimize gag, optimize the Kozak sequence previous the initiating methionine, and mutate an inside methionine codon to 1 for alanine (M100A) to forestall inside initiation of translation.
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Titanium Cup Tip, 250 ml
0-120-0019 250 ml
EUR 1377
Description: includes Interface Washers

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Description: includes Interface Washers

Continuous Flow Chamber
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Description: includes Interface Washers

Interface Washers 1.5” Diameter, 5/Pkg
0-120-003 1.5'' Diameter
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13 mm Diameter Solid Titanium Extender Tip
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EUR 301

13 mm Diameter Tapped Titanium Extender Tip
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19 mm Diameter Solid Titanium Extender Tip
0-120-0034 19 mm
EUR 315

19 mm Diameter Tapped Titanium Extender Tip
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25 mm Diameter Solid Titanium Extender Tip
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25 mm Diameter Tapped Titanium Extender Tip
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KoldPod, 1.5ml Micro Tube
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EUR 152
Description: Thermo conductive tube pods

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KoldPod, 50ml Conical Tube
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Model 150 V/T Ultrasonic Homogenizer
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Description: Delivers up to 150 Watts of ultrasonic power to the Titanium Tip. The Timer and Duty Cycle function increase preciosion in sample processing processing.

Model 300 V/T Ultrasonic Homogenizer
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SONABOZ Sound Abating Chamber
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Model 3000 Ultrasonic Homogenizer
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Model 3000MP Ultrasonic Homogenizer
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Description: Delivers up to 300 Watts of ultrasonic power to the Titanium Tip with preciosion control from a microprocessor and a graphical user interface displayed on a large (145 mm) LCD display. The integrated Sound Abating Chamber reduces cavitational sound emitted during processing.

OMNICON® Zone Reader, 210-240V/50-60Hz
0-131-0002 210-240V/50-60Hz
EUR 35200
Description: Designed to Perform multi-plate Assays on round 90/100mm Petri Dishes. The integrated LED illumination system provides transmitted light for brightfield and darkfield illumination of transparent media.

OMNI-Noculator Peni Cylinder Filler, 210-240V/50-60Hz
0-134-0002 210-240V/50-60Hz
EUR 32200
Description: A robotic liquid handling system designed to dispense Peni Cylinders and fill Peni Cylinders with the corresponding antibiotic liquid sample.

Peni Cylinder Dispenser with Manual Hopper
0-144-0002 each
EUR 5406
Description: Dispenser can be configured to dispense 4 or 6 Peni Cylinders onto a petri dish.

Peni Cylinder Dispenser with Motorized Hopper, 100-240V/50-60Hz
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EUR 6254
Description: The motorized hopper can be configured to dispense 4 or 6 Peni Cylinders onto a petri Dispenser can be disassembled for disinfection.

Stainless Steel Peni Cylinder with Flat Face
0-144-0005 6mm I.D. x 8mm O.D. x 10mm Long
EUR 399
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Stainless Steel Peni Cylinder with Chamfered Face
0-144-0006 6mm I.D. x 8mm O.D. x 10mm Long
EUR 412
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Custom development of ELISAs for other species or antibody isotypes not listed in the catalog. Custom testing of samples for IgG/IgM/IgA or total (IgG+IgM+IgA)
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SARS-CoV-2 Indicator Cell Line for RNA Replication - GFP Reporter only
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Tetramethylrhodamine-A-Bungarotoxin, 10x50 ug
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Sulforhodamine 101-Alpha-Bungarotoxin, 500 ug
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Biotin-cGMP, 20x50 ug
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Cyanine 644-cAMP, 1 mg
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Fluorescein Methotrexate, Triammonium Salt, 1 mg
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